Restriction map for
pFN21AB6685
(Circular) (Six-base) MAPSORT of: pFN21AB6685.seq Check: 640 from: 1 to: 5986
pFN21AB6685 5986 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2619 3796 278
Size: 53 83 186 936 1083 1177 2468
Fragments arranged by size:
2468 1177 1083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3270 3329 1937
Size: 1333 59 4594
Fragments arranged by size:
4594 1333 59
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2471 4049 5433 5701 1632
Size: 839 1578 1384 268 1917
Fragments arranged by size:
1917 1578 1384 839 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 2416 4885 5862 1203
Size: 1213 2469 977 1327
Fragments arranged by size:
2469 1327 1213 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 2122 3729 5301 2122
Size: 1607 1572 2807
Fragments arranged by size:
2807 1607 1572
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 2465 2929 3161 3295 3521 3673 5782 1493
Size: 972 464 232 134 226 152 2109 1697
Fragments arranged by size:
2109 1697 972 464 232 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 2215 2574 3311 1870
Size: 73 272 359 737 4545
Fragments arranged by size:
4545 737 359 272 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2731 3125 3307 4762 618
Size: 325 217 627 944 394 182 1455 1842
Fragments arranged by size:
1842 1455 944 627 394 325 217 182
BanII G_rGCy'C
Cuts at: 1391 2141 2607 3305 1391
Size: 750 466 698 4072
Fragments arranged by size:
4072 750 698 466
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5015
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3250 3284 4177 4211 1903
Size: 34 50 34 1229 34 893 34 3678
Fragments arranged by size:
3678 1229 893 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4597 136
Size: 107 122 71 631 605 2925 1525
Fragments arranged by size:
2925 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 2243 3403 2243
Size: 1160 4826
Bme1580I G_kGCm'C
Cuts at: 1790 2537 2736 4047 5203 1790
Size: 747 199 1311 1156 2573
Fragments arranged by size:
2573 1311 1156 747 199
BmgBI CAC'GTC
Cuts at: 1318 2560 3112 1318
Size: 1242 552 4192
Fragments arranged by size:
4192 1242 552
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3717 4675 442
Size: 416 53 83 399 24 2300 958 1753
Fragments arranged by size:
2300 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2871 3095 4646 1239
Size: 905 727 224 1551 2579
Fragments arranged by size:
2579 1551 905 727 224
Bpu10I CC'TnA_GC
Cuts at: 2438 2438
Size: 5986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2135 2330 2591 2665 4111 4976 5274 2135
Size: 195 261 74 1446 865 298 2847
Fragments arranged by size:
2847 1446 865 298 261 195 74
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2204 4649 882
Size: 617 705 2445 2219
Fragments arranged by size:
2445 2219 705 617
BsaAI yAC'GTr
Cuts at: 493 1204 3777 493
Size: 711 2573 2702
Fragments arranged by size:
2702 2573 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2616 3793 4175 4810
Size: 53 83 186 936 1083 1177 382 635
Cuts at: 4810 275
Size: 1451
Fragments arranged by size:
1451 1177 1083 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4419 4817 5091 5238 5649 4419
Size: 398 274 147 411 4756
Fragments arranged by size:
4756 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5761
Fragments arranged by size:
5761 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2018 1186
Size: 832 5154
BseYI C'CCAG_C
Cuts at: 2144 3010 5189 5685 2144
Size: 866 2179 496 2445
Fragments arranged by size:
2445 2179 866 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 2363 1163
Size: 573 318 309 4786
Fragments arranged by size:
4786 573 318 309
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3436 4197 5225 1540
Size: 275 186 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2431 2607 4047 4132 5203 2431
Size: 176 1440 85 1071 3214
Fragments arranged by size:
3214 1440 1071 176 85
BsmI GAATG_Cn'
Cuts at: 3497 3590 3497
Size: 93 5893
BsmBI CGTCTCn'nnnn_
Cuts at: 2814 2814
Size: 5986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2431 2537 2607 2736 3305 4047
Size: 399 351 290 106 70 129 569 742
Cuts at: 4047 4132 5203 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 569 399 351 290 129
106 85 70
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 2773 3875 5605 2773
Size: 1102 1730 3154
Fragments arranged by size:
3154 1730 1102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2031 3341 844
Size: 1187 1310 3489
Fragments arranged by size:
3489 1310 1187
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4481 4655 65
Size: 1183 3233 174 1396
Fragments arranged by size:
3233 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3204 4630 4817 1700
Size: 110 1394 1426 187 2869
Fragments arranged by size:
2869 1426 1394 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2377 794
Size: 1583 4403
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2304 3022 3316 3683 4068 4085 5537 5623
Size: 332 718 294 367 385 17 1452 86
Cuts at: 5623 5635 5981 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 718 385 367 346 332 294
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2401 2481 513
Size: 552 1336 80 4018
Fragments arranged by size:
4018 1336 552 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 2431 3502 4296 4316 2431
Size: 1071 794 20 4101
Fragments arranged by size:
4101 1071 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3290 3290
Size: 5986
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2398 2484
Size: 54 1425 50 275 18 232 336 86
Cuts at: 2484 3433 4322 5951 8
Size: 949 889 1629 43
Fragments arranged by size:
1629 1425 949 889 336 275 232 86
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2510 2868 2918 3916 1353
Size: 1157 358 50 998 3423
Fragments arranged by size:
3423 1157 998 358 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2605 2605
Size: 5986
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2471 2871 3095 4049 4646 5433
Size: 393 512 327 400 224 954 597 787
Cuts at: 5433 5701 1239
Size: 268 1524
Fragments arranged by size:
1524 954 787 597 512 400 393 327
268 224
EcoNI CCTnn'n_nnAGG
Cuts at: 2850 2850
Size: 5986
EcoO109I rG'GnC_Cy
Cuts at: 2280 2314 2280
Size: 34 5952
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2245 2635 3121 2245
Size: 390 486 5110
Fragments arranged by size:
5110 486 390
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 5133 1678
Size: 3455 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3014 3046 4627 4659 4701
Size: 32 135 32 1442 32 1581 32 42
Cuts at: 4701 4733 1373
Size: 32 2626
Fragments arranged by size:
2626 1581 1442 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3271 3330 3576 669
Size: 988 281 1333 59 246 3079
Fragments arranged by size:
3079 1333 988 281 246 59
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 2416 5862 2416
Size: 3446 2540
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2020 5100 5284 1305
Size: 715 3080 184 2007
Fragments arranged by size:
3080 2007 715 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2064 10
Size: 54 1425 343 232 3932
Fragments arranged by size:
3932 1425 343 232 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2085 3944 4303 4462 518
Size: 648 919 1859 359 159 2042
Fragments arranged by size:
2042 1859 919 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2242 3402 4079 5227 5472 1606
Size: 462 174 1160 677 1148 245 2120
Fragments arranged by size:
2120 1160 1148 677 462 245 174
NcoI C'CATG_G
Cuts at: 513 1065 2401 2481 513
Size: 552 1336 80 4018
Fragments arranged by size:
4018 1336 552 80
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NruI TCG'CGA
Cuts at: 3155 3155
Size: 5986
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 3344 4889 1132
Size: 97 2115 1545 2229
Fragments arranged by size:
2229 2115 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2275 1348
Size: 927 5059
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2407 1108
Size: 1299 4687
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PspXI vC'TCGA_Gb
Cuts at: 2574 2574
Size: 5986
PstI C_TGCA'G
Cuts at: 830 2748 3139 3338 830
Size: 1918 391 199 3478
Fragments arranged by size:
3478 1918 391 199
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
PvuII CAG'CTG
Cuts at: 2068 2068
Size: 5986
SacI G_AGCT'C
Cuts at: 2607 2607
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3269 3328 1936
Size: 1333 59 4594
Fragments arranged by size:
4594 1333 59
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 2744 3135 3334 4469 5150 5341 826
Size: 219 1699 391 199 1135 681 191 1471
Fragments arranged by size:
1699 1471 1135 681 391 219 199 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 2217 3313 1872
Size: 345 1096 4545
Fragments arranged by size:
4545 1096 345
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2150 2309 2574 2606 2680 4090
Size: 197 926 207 159 265 32 74 1410
Cuts at: 4090 4991 5253 820
Size: 901 262 1553
Fragments arranged by size:
1553 1410 926 901 265 262 207 197
159 74 32
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5986
StyI C'CwwG_G
Cuts at: 513 1065 2401 2481 2851 3105 3425 513
Size: 552 1336 80 370 254 320 3074
Fragments arranged by size:
3074 1336 552 370 320 254 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3254 4013 4030 4183 4368 4813 325
Size: 1255 1674 759 17 153 185 445 1498
Fragments arranged by size:
1674 1498 1255 759 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4232 5944 96
Size: 274 80 33 51 1443 2255 1712 138
Fragments arranged by size:
2255 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5946 5978 5946
Size: 32 5954
Tth111I GACn'n_nGTC
Cuts at: 1316 2558 1316
Size: 1242 4744
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2272 3109 2272
Size: 837 5149
XhoI C'TCGA_G
Cuts at: 1943 2574 1943
Size: 631 5355
XmaI C'CCGG_G
Cuts at: 1870 2215 3311 1870
Size: 345 1096 4545
Fragments arranged by size:
4545 1096 345
XmnI GAAnn'nnTTC
Cuts at: 2669 2877 4115 2669
Size: 208 1238 4540
Fragments arranged by size:
4540 1238 208
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2617 3794 276
Size: 53 83 186 936 1083 1177 2468
Fragments arranged by size:
2468 1177 1083 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI
PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI