Restriction map for
pFN21AB6683
(Circular) (Six-base) MAPSORT of: pFN21AB6683.seq Check: 866 from: 1 to: 5986
pFN21AB6683 5986 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3065 3065
Size: 5986
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3290 4049 5433 5701 1632
Size: 1658 759 1384 268 1917
Fragments arranged by size:
1917 1658 1384 759 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 4885 5862 1203
Size: 3682 977 1327
Fragments arranged by size:
3682 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 3729 5301 3729
Size: 1572 4414
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 2113 2511 2758 2861 3295 3521 3673 5782
Size: 620 398 247 103 434 226 152 2109
Cuts at: 5782 1493
Size: 1697
Fragments arranged by size:
2109 1697 620 434 398 247 226 152
103
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 2168 3311 1870
Size: 73 225 1143 4545
Fragments arranged by size:
4545 1143 225 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 3307 4762 618
Size: 325 217 627 291 1229 1455 1842
Fragments arranged by size:
1842 1455 1229 627 325 291 217
BanII G_rGCy'C
Cuts at: 1391 3305 1391
Size: 1914 4072
BbeI G_GCGC'C
Cuts at: 2082 2082
Size: 5986
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5015
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BfrBI ATG'CAT
Cuts at: 2355 2406 2355
Size: 51 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2020 4597 136
Size: 107 122 71 631 605 348 2577 1525
Fragments arranged by size:
2577 1525 631 605 348 122 107 71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 3403 3403
Size: 5986
Bme1580I G_kGCm'C
Cuts at: 1790 4047 5203 1790
Size: 2257 1156 2573
Fragments arranged by size:
2573 2257 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2299 3717 4675
Size: 416 53 83 399 24 882 1418 958
Cuts at: 4675 442
Size: 1753
Fragments arranged by size:
1753 1418 958 882 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3004 4646 1239
Size: 1765 1642 2579
Fragments arranged by size:
2579 1765 1642
Bpu10I CC'TnA_GC
Cuts at: 2420 2420
Size: 5986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3098 4111 4976 5274 3098
Size: 1013 865 298 3810
Fragments arranged by size:
3810 1013 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3168 4649 882
Size: 617 1669 1481 2219
Fragments arranged by size:
2219 1669 1481 617
BsaAI yAC'GTr
Cuts at: 493 1204 3777 493
Size: 711 2573 2702
Fragments arranged by size:
2702 2573 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 3793 4175 4810
Size: 53 83 186 936 546 1714 382 635
Cuts at: 4810 275
Size: 1451
Fragments arranged by size:
1714 1451 936 635 546 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2242 4419 4817 5091 5238 5649 2242
Size: 2177 398 274 147 411 2579
Fragments arranged by size:
2579 2177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5761
Fragments arranged by size:
5761 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5986
BseYI C'CCAG_C
Cuts at: 5189 5685 5189
Size: 496 5490
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2125 3179 1163
Size: 573 389 1054 3970
Fragments arranged by size:
3970 1054 573 389
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3436 4197 5225 1540
Size: 275 186 1435 761 1028 2301
Fragments arranged by size:
2301 1435 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4047 4132 5203 4047
Size: 85 1071 4830
Fragments arranged by size:
4830 1071 85
BsmI GAATG_Cn'
Cuts at: 3497 3590 3497
Size: 93 5893
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3305 4047 4132 5203 1391
Size: 399 1515 742 85 1071 2174
Fragments arranged by size:
2174 1515 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2242 5649 2242
Size: 3407 2579
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3065 3341 844
Size: 2221 276 3489
Fragments arranged by size:
3489 2221 276
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2747 4481 4655 65
Size: 1183 1499 1734 174 1396
Fragments arranged by size:
1734 1499 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2054 4630 4817 1700
Size: 110 204 40 2576 187 2869
Fragments arranged by size:
2869 2576 204 187 110 40
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5986
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5986
BstXI CCAn_nnnn'nTGG
Cuts at: 2552 2552
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 3316 3683 4068 4085 5537 5623 5635 5981
Size: 1344 367 385 17 1452 86 12 346
Cuts at: 5981 1972
Size: 1977
Fragments arranged by size:
1977 1452 1344 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4495
Fragments arranged by size:
4495 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 3502 4296 4316 3502
Size: 794 20 5172
Fragments arranged by size:
5172 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 3160 3290 3160
Size: 130 5856
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2012 3433 4322
Size: 54 1425 50 275 18 182 1421 889
Cuts at: 4322 5951 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1421 889 275 182 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3916 1353
Size: 2563 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3004 3290 4049 4646 5433 5701 1239
Size: 393 1372 286 759 597 787 268 1524
Fragments arranged by size:
1524 1372 787 759 597 393 286 268
EcoO109I rG'GnC_Cy
Cuts at: 2301 2301
Size: 5986
EcoRI G'AATT_C
Cuts at: 3295 3673 3295
Size: 378 5608
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 2042 2082 5133 1678
Size: 364 40 3051 2531
Fragments arranged by size:
3051 2531 364 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2146 2178 2272 2304 2366
Size: 32 135 32 574 32 94 32 62
Cuts at: 2366 2398 2870 2902 4627 4659 4701 4733 1373
Size: 32 472 32 1725 32 42 32 2626
Fragments arranged by size:
2626 1725 574 472 135 94 62 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3330 3576 669
Size: 988 281 1392 246 3079
Fragments arranged by size:
3079 1392 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KasI G'GCGC_C
Cuts at: 2078 2078
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5100 5284 1305
Size: 3795 184 2007
Fragments arranged by size:
3795 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4164
Fragments arranged by size:
4164 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2520 2724 3044 3944 4303 4462 518
Size: 648 1354 204 320 900 359 159 2042
Fragments arranged by size:
2042 1354 900 648 359 320 204 159
MspA1I CmG'CkG
Cuts at: 1606 3402 4079 5227 5472 1606
Size: 1796 677 1148 245 2120
Fragments arranged by size:
2120 1796 1148 677 245
NaeI GCC'GGC
Cuts at: 2016 2056 2016
Size: 40 5946
NarI GG'CG_CC
Cuts at: 2079 2079
Size: 5986
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4495
Fragments arranged by size:
4495 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NgoMIV G'CCGG_C
Cuts at: 2014 2054 2014
Size: 40 5946
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NsiI A_TGCA'T
Cuts at: 2357 2408 2357
Size: 51 5935
NspI r_CATG'y
Cuts at: 1132 1229 3344 4889 1132
Size: 97 2115 1545 2229
Fragments arranged by size:
2229 2115 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5986
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2617 1108
Size: 902 607 4477
Fragments arranged by size:
4477 902 607
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2301 2301
Size: 5986
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PstI C_TGCA'G
Cuts at: 830 3338 830
Size: 2508 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5986
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SanDI GG'GwC_CC
Cuts at: 2301 2301
Size: 5986
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 3090 4234 5946 3090
Size: 1144 1712 3130
Fragments arranged by size:
3130 1712 1144
SfcI C'TryA_G
Cuts at: 826 1045 3249 3334 4469 5150 5341 826
Size: 219 2204 85 1135 681 191 1471
Fragments arranged by size:
2204 1471 1135 681 219 191 85
SfoI GGC'GCC
Cuts at: 2080 2080
Size: 5986
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 2054 1700
Size: 354 5632
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3113 4090 4991 5253 820
Size: 197 926 1170 977 901 262 1553
Fragments arranged by size:
1553 1170 977 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5986
StyI C'CwwG_G
Cuts at: 513 1065 2004 3231 3425 513
Size: 552 939 1227 194 3074
Fragments arranged by size:
3074 1227 939 552 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4013 4030 4183 4368 4813 325
Size: 1255 2433 17 153 185 445 1498
Fragments arranged by size:
2433 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3088 4232 5944
Size: 274 80 33 51 1443 1111 1144 1712
Cuts at: 5944 96
Size: 138
Fragments arranged by size:
1712 1443 1144 1111 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5946 5978 5946
Size: 32 5954
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 2511 4115 2511
Size: 1604 4382
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI PacI PfoI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SapI SexAI
SfiI SrfI SwaI XcmI