Restriction map for
pFN21AB6679
(Circular) (Six-base) MAPSORT of: pFN21AB6679.seq Check: 8063 from: 1 to: 5986
pFN21AB6679 5986 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3796 278
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3329 1937
Size: 1392 4594
Acc65I G'GTAC_C
Cuts at: 3307 3307
Size: 5986
AclI AA'CG_TT
Cuts at: 1563 4113 4486 1563
Size: 2550 373 3063
Fragments arranged by size:
3063 2550 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2510 4049 5433 5701 1632
Size: 878 1539 1384 268 1917
Fragments arranged by size:
1917 1539 1384 878 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5789
AflIII A'CryG_T
Cuts at: 1203 3123 4885 5862 1203
Size: 1920 1762 977 1327
Fragments arranged by size:
1920 1762 1327 977
AgeI A'CCGG_T
Cuts at: 4817 4817
Size: 5986
AhdI GACnn_n'nnGTC
Cuts at: 4715 4715
Size: 5986
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5986
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5986
AlwNI CAG_nnn'CTG
Cuts at: 2191 3729 5301 2191
Size: 1538 1572 2876
Fragments arranged by size:
2876 1572 1538
ApaLI G'TGCA_C
Cuts at: 4043 5199 4043
Size: 1156 4830
ApoI r'AATT_y
Cuts at: 1493 2422 2808 3200 3295 3521 3673 5782 1493
Size: 929 386 392 95 226 152 2109 1697
Fragments arranged by size:
2109 1697 929 392 386 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 4540 160
Size: 872 3508 1606
Fragments arranged by size:
3508 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
AvaI C'yCGr_G
Cuts at: 1870 1943 2099 3311 1870
Size: 73 156 1212 4545
Fragments arranged by size:
4545 1212 156 73
BamHI G'GATC_C
Cuts at: 3316 3683 3316
Size: 367 5619
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2361 3307 4762 618
Size: 325 217 627 574 946 1455 1842
Fragments arranged by size:
1842 1455 946 627 574 325 217
BanII G_rGCy'C
Cuts at: 1391 2100 2129 2274 2908 3305 1391
Size: 709 29 145 634 397 4072
Fragments arranged by size:
4072 709 634 397 145 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2042 928
Size: 971 143 4872
Fragments arranged by size:
4872 971 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4177 4211 1903
Size: 34 2240 34 3678
Fragments arranged by size:
3678 2240 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3878 5088 3878
Size: 1210 4776
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5986
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2866 2976 4597
Size: 107 122 71 631 605 1194 110 1621
Cuts at: 4597 136
Size: 1525
Fragments arranged by size:
1621 1525 1194 631 605 122 110 107
71
BglII A'GATC_T
Cuts at: 5981 5981
Size: 5986
BlpI GC'TnA_GC
Cuts at: 3403 3403
Size: 5986
Bme1580I G_kGCm'C
Cuts at: 1790 4047 5203 1790
Size: 2257 1156 2573
Fragments arranged by size:
2573 2257 1156
BmgBI CAC'GTC
Cuts at: 1318 3124 1318
Size: 1806 4180
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2493 2953 3717
Size: 416 53 83 399 24 1076 460 764
Cuts at: 3717 4675 442
Size: 958 1753
Fragments arranged by size:
1753 1076 958 764 460 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5986
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2927
Size: 5986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2114 2258 2490 3277 4646 1239
Size: 869 6 144 232 787 1369 2579
Fragments arranged by size:
2579 1369 869 787 232 144 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4111 4976 5274 4111
Size: 865 298 4823
Fragments arranged by size:
4823 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4649 882
Size: 617 3150 2219
Fragments arranged by size:
3150 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3777 493
Size: 711 2573 2702
Fragments arranged by size:
2702 2573 711
BsaBI GATnn'nnATC
Cuts at: 5980 5980
Size: 5986
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3793 4175 4810 275
Size: 53 83 186 936 2260 382 635 1451
Fragments arranged by size:
2260 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2129 2307 4419 4817 5091 5238 5649 2129
Size: 178 2112 398 274 147 411 2466
Fragments arranged by size:
2466 2112 411 398 274 178 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2065 2095 2652 2682 1178
Size: 30 165 30 662 30 557 30 4482
Fragments arranged by size:
4482 662 557 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2086 2518 1186
Size: 900 432 4654
Fragments arranged by size:
4654 900 432
BseYI C'CCAG_C
Cuts at: 2236 2375 5189 5685 2236
Size: 139 2814 496 2537
Fragments arranged by size:
2814 2537 496 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5413
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3120 3436 4197 5225 1540
Size: 275 186 1119 316 761 1028 2301
Fragments arranged by size:
2301 1119 1028 761 316 275 186
BsiHKAI G_wGCw'C
Cuts at: 4047 4132 5203 4047
Size: 85 1071 4830
Fragments arranged by size:
4830 1071 85
BsmI GAATG_Cn'
Cuts at: 2727 3497 3590 2727
Size: 770 93 5123
Fragments arranged by size:
5123 770 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2129 2274 2908 3305 4047 4132
Size: 399 310 29 145 634 397 742 85
Cuts at: 4132 5203 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 634 399 397 310 145
85 29
BspEI T'CCGG_A
Cuts at: 5649 5649
Size: 5986
BspHI T'CATG_A
Cuts at: 3875 5605 3875
Size: 1730 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2826 3341 844
Size: 1982 515 3489
Fragments arranged by size:
3489 1982 515
BsrBI CCG'CTC
Cuts at: 1394 1823 3873 1394
Size: 429 2050 3507
Fragments arranged by size:
3507 2050 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3240 4481 4655 65
Size: 1183 1992 1241 174 1396
Fragments arranged by size:
1992 1396 1241 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4630 4817 1700
Size: 110 2820 187 2869
Fragments arranged by size:
2869 2820 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5986
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5986
BssSI C'ACGA_G
Cuts at: 4046 5058 4046
Size: 1012 4974
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5986
BstBI TT'CG_AA
Cuts at: 4790 4790
Size: 5986
BstEII G'GTnAC_C
Cuts at: 1184 3239 1184
Size: 2055 3931
BstXI CCAn_nnnn'nTGG
Cuts at: 2383 2383
Size: 5986
BstYI r'GATC_y
Cuts at: 1972 2187 2937 3076 3316 3683 4068 4085 5537
Size: 215 750 139 240 367 385 17 1452
Cuts at: 5537 5623 5635 5981 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 750 385 367 346 240 215
139 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2382 2919 3210 513
Size: 552 939 378 537 291 3289
Fragments arranged by size:
3289 939 552 537 378 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5247
Fragments arranged by size:
5247 683 56
BtsI GCAGTG_nn'
Cuts at: 3502 4296 4316 3502
Size: 794 20 5172
Fragments arranged by size:
5172 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5986
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5951
DraI TTT'AAA
Cuts at: 2563 3290 2563
Size: 727 5259
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4993 809
Size: 507 3677 1802
Fragments arranged by size:
3677 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2252 2778 3433
Size: 54 1425 50 275 18 422 526 655
Cuts at: 3433 4322 5951 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 655 526 422 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3433 1537
Size: 275 1621 4090
Fragments arranged by size:
4090 1621 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2924 3916 1353
Size: 1571 992 3423
Fragments arranged by size:
3423 1571 992
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4573 4947 5093 387
Size: 1064 138 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2114 2258 2490 2510 3277 4049
Size: 393 476 6 144 232 20 767 772
Cuts at: 4049 4646 5433 5701 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 772 767 597 476 393 268
232 144 20 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2335 2822 2335
Size: 487 5499
EcoO109I rG'GnC_Cy
Cuts at: 2217 2815 2217
Size: 598 5388
EcoRI G'AATT_C
Cuts at: 3200 3295 3673 3200
Size: 95 378 5513
Fragments arranged by size:
5513 378 95
EcoRV GAT'ATC
Cuts at: 2410 2410
Size: 5986
FspI TGC'GCA
Cuts at: 4492 4492
Size: 5986
HaeII r_GCGC'y
Cuts at: 1678 5133 1678
Size: 3455 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2065 2097 2463 2495 3122
Size: 32 135 32 493 32 366 32 627
Cuts at: 3122 3139 3154 3158 3171 3190 4627 4659 4701
Size: 17 15 4 13 19 1437 32 42
Cuts at: 4701 4733 1373
Size: 32 2626
Fragments arranged by size:
2626 1437 627 493 366 135 42 32
32 32 32 32 32 19 17 15
13 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2878 3330 3576 669
Size: 988 281 940 452 246 3079
Fragments arranged by size:
3079 988 940 452 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5986
HpaI GTT'AAC
Cuts at: 3576 3576
Size: 5986
KpnI G_GTAC'C
Cuts at: 3311 3311
Size: 5986
MfeI C'AATT_G
Cuts at: 3585 3585
Size: 5986
MluI A'CGCG_T
Cuts at: 5862 5862
Size: 5986
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5100 5284 1305
Size: 3795 184 2007
Fragments arranged by size:
3795 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2254 2780 10
Size: 54 1425 343 422 526 3216
Fragments arranged by size:
3216 1425 526 422 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3059 3944 4303 4462 518
Size: 648 1893 885 359 159 2042
Fragments arranged by size:
2042 1893 885 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3402 4079 5227 5472 1606
Size: 1796 677 1148 245 2120
Fragments arranged by size:
2120 1796 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3210 513
Size: 552 939 1206 3289
Fragments arranged by size:
3289 1206 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5986
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5986
NotI GC'GGCC_GC
Cuts at: 3433 3433
Size: 5986
NspI r_CATG'y
Cuts at: 1132 1229 2747 3344 4889 1132
Size: 97 1518 597 1545 2229
Fragments arranged by size:
2229 1545 1518 597 97
PasI CC'CwG_GG
Cuts at: 1348 2974 1348
Size: 1626 4360
PciI A'CATG_T
Cuts at: 4885 4885
Size: 5986
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5986
PmeI GTTT'AAAC
Cuts at: 3290 3290
Size: 5986
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4055 4087 5568 5600 4055
Size: 32 1481 32 4441
Fragments arranged by size:
4441 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2217 2815 2217
Size: 598 5388
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5986
PsiI TTA'TAA
Cuts at: 3556 3556
Size: 5986
PstI C_TGCA'G
Cuts at: 830 3338 830
Size: 2508 3478
PvuI CG_AT'CG
Cuts at: 2001 3120 2001
Size: 1119 4867
SalI G'TCGA_C
Cuts at: 1936 3328 1936
Size: 1392 4594
SapI GCTCTTCn'nnn_
Cuts at: 2924 2924
Size: 5986
SbfI CC_TGCA'GG
Cuts at: 3338 3338
Size: 5986
ScaI AGT'ACT
Cuts at: 4234 5946 4234
Size: 1712 4274
SfcI C'TryA_G
Cuts at: 826 1045 3334 4469 5150 5341 826
Size: 219 2289 1135 681 191 1471
Fragments arranged by size:
2289 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5986
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5986
SmaI CCC'GGG
Cuts at: 1872 3313 1872
Size: 1441 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4090 4991 5253 820
Size: 197 926 2147 901 262 1553
Fragments arranged by size:
2147 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3777 493
Size: 3284 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5986
SphI G_CATG'C
Cuts at: 1132 3344 1132
Size: 2212 3774
SspI AAT'ATT
Cuts at: 5 52 3910 5
Size: 47 3858 2081
Fragments arranged by size:
3858 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5986
StyI C'CwwG_G
Cuts at: 513 1065 2004 2352 3210 3425 513
Size: 552 939 348 858 215 3074
Fragments arranged by size:
3074 939 858 552 348 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2155 2379 3034 4013 4030 4183 4368
Size: 1255 575 224 655 979 17 153 185
Cuts at: 4368 4813 325
Size: 445 1498
Fragments arranged by size:
1498 1255 979 655 575 445 224 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4232 5944 96
Size: 274 80 33 51 1443 2255 1712 138
Fragments arranged by size:
2255 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2652 2684 5946 5978 2652
Size: 32 3262 32 2660
Fragments arranged by size:
3262 2660 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5986
XbaI T'CTAG_A
Cuts at: 3322 3322
Size: 5986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2766 2766
Size: 5986
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5986
XmaI C'CCGG_G
Cuts at: 1870 3311 1870
Size: 1441 4545
XmnI GAAnn'nnTTC
Cuts at: 4115 4115
Size: 5986
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3794 276
Size: 53 83 186 936 2260 2468
Fragments arranged by size:
2468 2260 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI