Restriction map for
pFN21AB6678
(Circular) (Six-base) MAPSORT of: pFN21AB6678.seq Check: 9082 from: 1 to: 6013
pFN21AB6678 6013 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3823 278
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3356 1937
Size: 1419 4594
Acc65I G'GTAC_C
Cuts at: 3334 3334
Size: 6013
AclI AA'CG_TT
Cuts at: 1563 4140 4513 1563
Size: 2577 373 3063
Fragments arranged by size:
3063 2577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2059 2071 2342 3143 4076 5460 5728 1632
Size: 427 12 271 801 933 1384 268 1917
Fragments arranged by size:
1917 1384 933 801 427 271 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5816
AflIII A'CryG_T
Cuts at: 1203 3006 4912 5889 1203
Size: 1803 1906 977 1327
Fragments arranged by size:
1906 1803 1327 977
AgeI A'CCGG_T
Cuts at: 4844 4844
Size: 6013
AhdI GACnn_n'nnGTC
Cuts at: 4742 4742
Size: 6013
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6013
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2955 2987 2955
Size: 32 5981
AlwNI CAG_nnn'CTG
Cuts at: 2191 2426 3756 5328 2191
Size: 235 1330 1572 2876
Fragments arranged by size:
2876 1572 1330 235
ApaI G_GGCC'C
Cuts at: 2054 2054
Size: 6013
ApaLI G'TGCA_C
Cuts at: 4070 5226 4070
Size: 1156 4857
ApoI r'AATT_y
Cuts at: 1493 3322 3548 3700 5809 1493
Size: 1829 226 152 2109 1697
Fragments arranged by size:
2109 1829 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4567 160
Size: 872 3535 1606
Fragments arranged by size:
3535 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2053 2098 3338 1870
Size: 73 104 6 45 1240 4545
Fragments arranged by size:
4545 1240 104 73 45 6
BamHI G'GATC_C
Cuts at: 2512 3343 3710 2512
Size: 831 367 4815
Fragments arranged by size:
4815 831 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3334 4789 618
Size: 325 217 627 1547 1455 1842
Fragments arranged by size:
1842 1547 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 2105 3332 1391
Size: 663 51 1227 4072
Fragments arranged by size:
4072 1227 663 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2348 928
Size: 971 449 4593
Fragments arranged by size:
4593 971 449
BbvCI CC'TCA_GC
Cuts at: 2391 2391
Size: 6013
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4204 4238 1903
Size: 34 2267 34 3678
Fragments arranged by size:
3678 2267 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3905 5115 3905
Size: 1210 4803
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 4624 136
Size: 107 122 71 631 605 367 2585 1525
Fragments arranged by size:
2585 1525 631 605 367 122 107 71
BglII A'GATC_T
Cuts at: 6008 6008
Size: 6013
BlpI GC'TnA_GC
Cuts at: 3430 3430
Size: 6013
Bme1580I G_kGCm'C
Cuts at: 1790 2054 4074 5230 1790
Size: 264 2020 1156 2573
Fragments arranged by size:
2573 2020 1156 264
BmgBI CAC'GTC
Cuts at: 1318 3092 1318
Size: 1774 4239
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3200 3744 4702
Size: 416 53 83 399 24 1783 544 958
Cuts at: 4702 442
Size: 1753
Fragments arranged by size:
1783 1753 958 544 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2089 4673 1239
Size: 850 2584 2579
Fragments arranged by size:
2584 2579 850
Bpu10I CC'TnA_GC
Cuts at: 2391 2391
Size: 6013
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4138 5003 5301 4138
Size: 865 298 4850
Fragments arranged by size:
4850 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3032 3111 4676 882
Size: 617 1533 79 1565 2219
Fragments arranged by size:
2219 1565 1533 617 79
BsaAI yAC'GTr
Cuts at: 493 1204 3804 493
Size: 711 2600 2702
Fragments arranged by size:
2702 2600 711
BsaBI GATnn'nnATC
Cuts at: 2435 6007 2435
Size: 3572 2441
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2114 2716 3820 4202
Size: 53 83 186 936 581 602 1104 382
Cuts at: 4202 4837 275
Size: 635 1451
Fragments arranged by size:
1451 1104 936 635 602 581 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4446 4844 5118 5265 5676 4446
Size: 398 274 147 411 4783
Fragments arranged by size:
4783 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2087 2117 2955 2985 1178
Size: 30 165 30 684 30 838 30 4206
Fragments arranged by size:
4206 838 684 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2976 1186
Size: 1790 4223
BseYI C'CCAG_C
Cuts at: 2923 3272 5216 5712 2923
Size: 349 1944 496 3224
Fragments arranged by size:
3224 1944 496 349
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3003 1163
Size: 573 1267 4173
Fragments arranged by size:
4173 1267 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3463 4224 5252 1540
Size: 275 186 1462 761 1028 2301
Fragments arranged by size:
2301 1462 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4074 4159 5230 4074
Size: 85 1071 4857
Fragments arranged by size:
4857 1071 85
BsmI GAATG_Cn'
Cuts at: 2215 2857 3524 3617 2215
Size: 642 667 93 4611
Fragments arranged by size:
4611 667 642 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2105 3332 4074 4159 5230 1391
Size: 399 264 51 1227 742 85 1071 2174
Fragments arranged by size:
2174 1227 1071 742 399 264 85 51
BspEI T'CCGG_A
Cuts at: 5676 5676
Size: 6013
BspHI T'CATG_A
Cuts at: 3902 5632 3902
Size: 1730 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3368 844
Size: 2524 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2173 3900 1394
Size: 429 350 1727 3507
Fragments arranged by size:
3507 1727 429 350
BsrDI GCAATG_nn'
Cuts at: 65 1248 2680 2862 4508 4682 65
Size: 1183 1432 182 1646 174 1396
Fragments arranged by size:
1646 1432 1396 1183 182 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 2069 4657 4844 1700
Size: 110 232 27 2588 187 2869
Fragments arranged by size:
2869 2588 232 187 110 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6013
BssHII G'CGCG_C
Cuts at: 1925 2062 2153 1925
Size: 137 91 5785
Fragments arranged by size:
5785 137 91
BssSI C'ACGA_G
Cuts at: 3030 3248 4073 5085 3030
Size: 218 825 1012 3958
Fragments arranged by size:
3958 1012 825 218
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2251 794
Size: 1457 4556
BstBI TT'CG_AA
Cuts at: 4817 4817
Size: 6013
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6013
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6013
BstYI r'GATC_y
Cuts at: 1972 2512 3343 3710 4095 4112 5564 5650 5662
Size: 540 831 367 385 17 1452 86 12
Cuts at: 5662 6008 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 831 540 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5461
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5274
Fragments arranged by size:
5274 683 56
BtsI GCAGTG_nn'
Cuts at: 3057 3313 3529 4323 4343 3057
Size: 256 216 794 20 4727
Fragments arranged by size:
4727 794 256 216 20
ClaI AT'CG_AT
Cuts at: 1554 2551 1554
Size: 997 5016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2781 2816 529
Size: 35 2217 35 3726
Fragments arranged by size:
3726 2217 35 35
DraI TTT'AAA
Cuts at: 3317 3317
Size: 6013
DraIII CAC_nnn'GTG
Cuts at: 2833 2833
Size: 6013
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3266 5020 809
Size: 507 1950 1754 1802
Fragments arranged by size:
1950 1802 1754 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 3460 4349
Size: 54 1425 50 275 18 210 1420 889
Cuts at: 4349 5978 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1420 889 275 210 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3460 1537
Size: 275 1648 4090
Fragments arranged by size:
4090 1648 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3943 1353
Size: 2590 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4600 4974 5120 387
Size: 1064 138 3011 374 146 1280
Fragments arranged by size:
3011 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2071 2089 2342 3143 4076 4673
Size: 393 427 12 18 253 801 933 597
Cuts at: 4673 5460 5728 1239
Size: 787 268 1524
Fragments arranged by size:
1524 933 801 787 597 427 393 268
253 18 12
EcoRI G'AATT_C
Cuts at: 3322 3700 3322
Size: 378 5635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2518 2518
Size: 6013
FseI GG_CCGG'CC
Cuts at: 2046 2046
Size: 6013
FspI TGC'GCA
Cuts at: 4519 4519
Size: 6013
HaeII r_GCGC'y
Cuts at: 1678 5160 1678
Size: 3482 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2186 2218 4654
Size: 32 135 32 515 32 67 32 2436
Cuts at: 4654 4686 4728 4760 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2436 515 135 67 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 3357 3603 669
Size: 988 281 1245 174 246 3079
Fragments arranged by size:
3079 1245 988 281 246 174
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6013
HpaI GTT'AAC
Cuts at: 3603 3603
Size: 6013
KpnI G_GTAC'C
Cuts at: 3338 3338
Size: 6013
MfeI C'AATT_G
Cuts at: 2327 3612 2327
Size: 1285 4728
MluI A'CGCG_T
Cuts at: 5889 5889
Size: 6013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5127 5311 1305
Size: 3822 184 2007
Fragments arranged by size:
3822 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4191
Fragments arranged by size:
4191 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2454 3971 4330 4489 518
Size: 648 1288 1517 359 159 2042
Fragments arranged by size:
2042 1517 1288 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3429 4106 5254 5499 1606
Size: 1823 677 1148 245 2120
Fragments arranged by size:
2120 1823 1148 677 245
NaeI GCC'GGC
Cuts at: 2044 2071 2044
Size: 27 5986
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5461
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6013
NgoMIV G'CCGG_C
Cuts at: 2042 2069 2042
Size: 27 5986
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6013
NotI GC'GGCC_GC
Cuts at: 3460 3460
Size: 6013
NspI r_CATG'y
Cuts at: 1132 1229 3010 3371 4916 1132
Size: 97 1781 361 1545 2229
Fragments arranged by size:
2229 1781 1545 361 97
PasI CC'CwG_GG
Cuts at: 1348 2476 1348
Size: 1128 4885
PciI A'CATG_T
Cuts at: 3006 4912 3006
Size: 1906 4107
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6013
PfoI T'CCnGG_A
Cuts at: 2190 2190
Size: 6013
PmeI GTTT'AAAC
Cuts at: 3317 3317
Size: 6013
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2955 2987 4082 4114 5595 5627 2955
Size: 32 1095 32 1481 32 3341
Fragments arranged by size:
3341 1481 1095 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6013
PsiI TTA'TAA
Cuts at: 3583 3583
Size: 6013
PspOMI G'GGCC_C
Cuts at: 2050 2050
Size: 6013
PstI C_TGCA'G
Cuts at: 830 3365 830
Size: 2535 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6013
SalI G'TCGA_C
Cuts at: 1936 3355 1936
Size: 1419 4594
SbfI CC_TGCA'GG
Cuts at: 3365 3365
Size: 6013
ScaI AGT'ACT
Cuts at: 2540 4261 5973 2540
Size: 1721 1712 2580
Fragments arranged by size:
2580 1721 1712
SfcI C'TryA_G
Cuts at: 826 1045 3361 4496 5177 5368 826
Size: 219 2316 1135 681 191 1471
Fragments arranged by size:
2316 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6013
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6013
SmaI CCC'GGG
Cuts at: 1872 2049 2055 3340 1872
Size: 177 6 1285 4545
Fragments arranged by size:
4545 1285 177 6
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4117 5018 5280 820
Size: 197 926 2174 901 262 1553
Fragments arranged by size:
2174 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3804 493
Size: 3311 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6013
SphI G_CATG'C
Cuts at: 1132 3371 1132
Size: 2239 3774
SrfI GCCC'GGGC
Cuts at: 2049 2049
Size: 6013
SspI AAT'ATT
Cuts at: 5 52 3937 5
Size: 47 3885 2081
Fragments arranged by size:
3885 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6013
StyI C'CwwG_G
Cuts at: 513 1065 2037 3112 3452 513
Size: 552 972 1075 340 3074
Fragments arranged by size:
3074 1075 972 552 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2973 4040 4057 4210 4395 4840 325
Size: 1255 1393 1067 17 153 185 445 1498
Fragments arranged by size:
1498 1393 1255 1067 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2538 2677 4259
Size: 274 80 33 51 1443 561 139 1582
Cuts at: 4259 5971 96
Size: 1712 138
Fragments arranged by size:
1712 1582 1443 561 274 139 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3093 3125 5973 6005 3093
Size: 32 2848 32 3101
Fragments arranged by size:
3101 2848 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6013
XbaI T'CTAG_A
Cuts at: 3349 3349
Size: 6013
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6013
XmaI C'CCGG_G
Cuts at: 1870 2047 2053 3338 1870
Size: 177 6 1285 4545
Fragments arranged by size:
4545 1285 177 6
XmnI GAAnn'nnTTC
Cuts at: 2186 4142 2186
Size: 1956 4057
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3821 276
Size: 53 83 186 936 2287 2468
Fragments arranged by size:
2468 2287 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV
FspAI KasI NarI NruI NsiI PacI PmlI PpuMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SwaI XcmI