Restriction map for
pFN21AB6670
(Circular) (Six-base) MAPSORT of: pFN21AB6670.seq Check: 7085 from: 1 to: 6067
pFN21AB6670 6067 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3410 1937
Size: 1473 4594
Acc65I G'GTAC_C
Cuts at: 3102 3388 3102
Size: 286 5781
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2444 2710 2915 2930 3226 4130 5514 5782
Size: 812 266 205 15 296 904 1384 268
Cuts at: 5782 1632
Size: 1917
Fragments arranged by size:
1917 1384 904 812 296 268 266 205
15
AfeI AGC'GCT
Cuts at: 2975 2975
Size: 6067
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 2601 2903 4966 5943 1203
Size: 1398 302 2063 977 1327
Fragments arranged by size:
2063 1398 1327 977 302
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 2904 4796 2904
Size: 1892 4175
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 2374 2812 3136 3157 3810 5382 2374
Size: 438 324 21 653 1572 3059
Fragments arranged by size:
3059 1572 653 438 324 21
ApaLI G'TGCA_C
Cuts at: 4124 5280 4124
Size: 1156 4911
ApoI r'AATT_y
Cuts at: 1493 3376 3602 3754 5863 1493
Size: 1883 226 152 2109 1697
Fragments arranged by size:
2109 1883 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 2879 3392 1870
Size: 73 936 513 4545
Fragments arranged by size:
4545 936 513 73
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2220 2540 2889 3102 3388
Size: 325 217 627 433 320 349 213 286
Cuts at: 3388 4843 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 433 349 325 320 286
217 213
BanII G_rGCy'C
Cuts at: 1391 2307 3386 1391
Size: 916 1079 4072
Fragments arranged by size:
4072 1079 916
BbeI G_GCGC'C
Cuts at: 2893 2893
Size: 6067
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2903 928
Size: 971 1004 4092
Fragments arranged by size:
4092 1004 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4258 4292 1903
Size: 34 2321 34 3678
Fragments arranged by size:
3678 2321 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2884 4678 136
Size: 107 122 71 631 605 1212 1794 1525
Fragments arranged by size:
1794 1525 1212 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6062 6062
Size: 6067
BlpI GC'TnA_GC
Cuts at: 3484 3484
Size: 6067
Bme1580I G_kGCm'C
Cuts at: 1790 2108 2463 4128 5284 1790
Size: 318 355 1665 1156 2573
Fragments arranged by size:
2573 1665 1156 355 318
BmgBI CAC'GTC
Cuts at: 1318 2029 2269 3061 1318
Size: 711 240 792 4324
Fragments arranged by size:
4324 792 711 240
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2350 3798 4756
Size: 416 53 83 399 24 933 1448 958
Cuts at: 4756 442
Size: 1753
Fragments arranged by size:
1753 1448 958 933 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2995 2308
Size: 687 5380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 2612 2920 3011 3272 3347 4727 1239
Size: 1064 309 308 91 261 75 1380 2579
Fragments arranged by size:
2579 1380 1064 309 308 261 91 75
Bpu10I CC'TnA_GC
Cuts at: 2636 2636
Size: 6067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2348 3146 3161 3215 4192 5057 5355 2348
Size: 798 15 54 977 865 298 3060
Fragments arranged by size:
3060 977 865 798 298 54 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2296 2657 4730 882
Size: 617 797 361 2073 2219
Fragments arranged by size:
2219 2073 797 617 361
BsaAI yAC'GTr
Cuts at: 493 1204 3858 493
Size: 711 2654 2702
Fragments arranged by size:
2702 2654 711
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2890 3874 4256 4891
Size: 53 83 186 936 1357 984 382 635
Cuts at: 4891 275
Size: 1451
Fragments arranged by size:
1451 1357 984 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4500 4898 5172 5319 5730 4500
Size: 398 274 147 411 4837
Fragments arranged by size:
4837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 2599 2629 3086
Size: 30 165 30 912 30 254 30 457
Cuts at: 3086 3116 1178
Size: 30 4129
Fragments arranged by size:
4129 912 457 254 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2069 2195 2732 3008 3026 3101 3125 3209
Size: 883 126 537 276 18 75 24 84
Cuts at: 3209 1186
Size: 4044
Fragments arranged by size:
4044 883 537 276 126 84 75 24
18
BseYI C'CCAG_C
Cuts at: 2059 2584 3214 5270 5766 2059
Size: 525 630 2056 496 2360
Fragments arranged by size:
2360 2056 630 525 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 2378 3326 1163
Size: 573 489 153 948 3904
Fragments arranged by size:
3904 948 573 489 153
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2082 2872 3006 3517 4278 5306
Size: 275 186 81 790 134 511 761 1028
Cuts at: 5306 1540
Size: 2301
Fragments arranged by size:
2301 1028 790 761 511 275 186 134
81
BsiHKAI G_wGCw'C
Cuts at: 2307 4128 4213 5284 2307
Size: 1821 85 1071 3090
Fragments arranged by size:
3090 1821 1071 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
BsmBI CGTCTCn'nnnn_
Cuts at: 2669 2669
Size: 6067
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 2307 2463 3386 4128 4213 5284
Size: 399 318 199 156 923 742 85 1071
Cuts at: 5284 1391
Size: 2174
Fragments arranged by size:
2174 1071 923 742 399 318 199 156
85
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 3956 5686 3956
Size: 1730 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2400 3144 3422 844
Size: 1556 744 278 3489
Fragments arranged by size:
3489 1556 744 278
BsrBI CCG'CTC
Cuts at: 1394 1823 2191 2640 2884 3954 1394
Size: 429 368 449 244 1070 3507
Fragments arranged by size:
3507 1070 449 429 368 244
BsrDI GCAATG_nn'
Cuts at: 65 1248 2799 4562 4736 65
Size: 1183 1551 1763 174 1396
Fragments arranged by size:
1763 1551 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2094 2160 2763 3039 4711 4898 1700
Size: 110 284 66 603 276 1672 187 2869
Fragments arranged by size:
2869 1672 603 284 276 187 110 66
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6067
BssHII G'CGCG_C
Cuts at: 1925 3207 3319 1925
Size: 1282 112 4673
Fragments arranged by size:
4673 1282 112
BssSI C'ACGA_G
Cuts at: 3053 3314 4127 5139 3053
Size: 261 813 1012 3981
Fragments arranged by size:
3981 1012 813 261
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3157 794
Size: 2363 3704
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6067
BstXI CCAn_nnnn'nTGG
Cuts at: 2743 2989 2743
Size: 246 5821
BstYI r'GATC_y
Cuts at: 1972 2214 2370 2779 3397 3764 4149 4166 5618
Size: 242 156 409 618 367 385 17 1452
Cuts at: 5618 5704 5716 6062 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 618 409 385 367 346 242
156 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5515
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2392 2479 487
Size: 683 56 1166 87 4075
Fragments arranged by size:
4075 1166 683 87 56
BtsI GCAGTG_nn'
Cuts at: 3583 4377 4397 3583
Size: 794 20 5253
Fragments arranged by size:
5253 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5074 809
Size: 507 3758 1802
Fragments arranged by size:
3758 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2068 2745 2869
Size: 54 1425 50 275 18 238 677 124
Cuts at: 2869 3003 3072 3273 3514 4403 6032 8
Size: 134 69 201 241 889 1629 43
Fragments arranged by size:
1629 1425 889 677 275 241 238 201
134 124 69 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2869 3003 3514 1537
Size: 275 1057 134 511 4090
Fragments arranged by size:
4090 1057 511 275 134
EarI CTCTTCn'nnn_
Cuts at: 1353 3997 1353
Size: 2644 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2336 2612 2860 4654 5028 5174
Size: 1064 138 747 276 248 1794 374 146
Cuts at: 5174 387
Size: 1280
Fragments arranged by size:
1794 1280 1064 747 374 276 248 146
138
EcoICRI GAG'CTC
Cuts at: 2305 2305
Size: 6067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 2444 2612 2710 2915 2920 2930
Size: 393 671 141 168 98 205 5 10
Cuts at: 2930 3011 3226 3272 3347 4130 4727 5514 5782
Size: 81 215 46 75 783 597 787 268
Cuts at: 5782 1239
Size: 1524
Fragments arranged by size:
1524 787 783 671 597 393 268 215
205 168 141 98 81 75 46 10
5
EcoNI CCTnn'n_nnAGG
Cuts at: 3128 3329 3128
Size: 201 5866
EcoO109I rG'GnC_Cy
Cuts at: 3016 3133 3310 3016
Size: 117 177 5773
Fragments arranged by size:
5773 177 117
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2551 3256 2551
Size: 705 5362
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 2146 2893 2977 3071 3176 3238 3272 5214
Size: 468 747 84 94 105 62 34 1942
Cuts at: 5214 1678
Size: 2531
Fragments arranged by size:
2531 1942 747 468 105 94 84 62
34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2890 2922 4708
Size: 32 135 32 743 32 543 32 1786
Cuts at: 4708 4740 4782 4814 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1786 743 543 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KasI G'GCGC_C
Cuts at: 2889 2889
Size: 6067
KpnI G_GTAC'C
Cuts at: 3106 3392 3106
Size: 286 5781
MfeI C'AATT_G
Cuts at: 3666 3666
Size: 6067
MluI A'CGCG_T
Cuts at: 2903 5943 2903
Size: 3040 3027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5181 5365 1305
Size: 3876 184 2007
Fragments arranged by size:
3876 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2747 3275 10
Size: 54 1425 343 915 528 2802
Fragments arranged by size:
2802 1425 915 528 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4025 4384 4543 518
Size: 648 2859 359 159 2042
Fragments arranged by size:
2859 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2067 2584 2868 3225 3483 4160 5308 5553
Size: 461 517 284 357 258 677 1148 245
Cuts at: 5553 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 517 461 357 284 258
245
NaeI GCC'GGC
Cuts at: 2096 2162 2765 3041 2096
Size: 66 603 276 5122
Fragments arranged by size:
5122 603 276 66
NarI GG'CG_CC
Cuts at: 2890 2890
Size: 6067
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5515
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6067
NgoMIV G'CCGG_C
Cuts at: 2094 2160 2763 3039 2094
Size: 66 603 276 5122
Fragments arranged by size:
5122 603 276 66
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 2869 3003 3514 2869
Size: 134 511 5422
Fragments arranged by size:
5422 511 134
NspI r_CATG'y
Cuts at: 1132 1229 2605 3425 4970 1132
Size: 97 1376 820 1545 2229
Fragments arranged by size:
2229 1545 1376 820 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6067
PciI A'CATG_T
Cuts at: 2601 4966 2601
Size: 2365 3702
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2812 1108
Size: 1704 4363
PfoI T'CCnGG_A
Cuts at: 2280 2373 2822 2280
Size: 93 449 5525
Fragments arranged by size:
5525 449 93
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 5649 5681 4136
Size: 32 1481 32 4522
Fragments arranged by size:
4522 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3133 3310 3133
Size: 177 5890
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PstI C_TGCA'G
Cuts at: 830 3033 3345 3419 830
Size: 2203 312 74 3478
Fragments arranged by size:
3478 2203 312 74
PvuI CG_AT'CG
Cuts at: 2001 2082 2001
Size: 81 5986
SacI G_AGCT'C
Cuts at: 2307 2307
Size: 6067
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 2723 4315 6027 2723
Size: 1592 1712 2763
Fragments arranged by size:
2763 1712 1592
SfcI C'TryA_G
Cuts at: 826 1045 3029 3341 3415 4550 5231 5422 826
Size: 219 1984 312 74 1135 681 191 1471
Fragments arranged by size:
1984 1471 1135 681 312 219 191 74
SfoI GGC'GCC
Cuts at: 2891 2891
Size: 6067
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2363 3161 3176 3230 4171 5072
Size: 197 926 420 798 15 54 941 901
Cuts at: 5072 5334 820
Size: 262 1553
Fragments arranged by size:
1553 941 926 901 798 420 262 197
54 15
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 3098 1517
Size: 1581 4486
StyI C'CwwG_G
Cuts at: 513 1065 2748 2982 3201 3506 513
Size: 552 1683 234 219 305 3074
Fragments arranged by size:
3074 1683 552 305 234 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2369 3182 4094 4111 4264 4449 4894
Size: 1255 789 813 912 17 153 185 445
Cuts at: 4894 325
Size: 1498
Fragments arranged by size:
1498 1255 912 813 789 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2721 2916 4313
Size: 274 80 33 51 1443 744 195 1397
Cuts at: 4313 6025 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1397 744 274 195 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2599 2631 2839 2871 6027 6059 2599
Size: 32 208 32 3156 32 2607
Fragments arranged by size:
3156 2607 208 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2270 1316
Size: 954 5113
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 2522 2534 4196 2522
Size: 12 1662 4393
Fragments arranged by size:
4393 1662 12
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI