Restriction map for
pFN21AB6667
(Circular) (Six-base) MAPSORT of: pFN21AB6667.seq Check: 7318 from: 1 to: 5980
pFN21AB6667 5980 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3790 278
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3323 1937
Size: 1386 4594
Acc65I G'GTAC_C
Cuts at: 3301 3301
Size: 5980
AclI AA'CG_TT
Cuts at: 1563 4107 4480 1563
Size: 2544 373 3063
Fragments arranged by size:
3063 2544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2355 2880 4043 5427 5695 1632
Size: 723 525 1163 1384 268 1917
Fragments arranged by size:
1917 1384 1163 723 525 268
AfeI AGC'GCT
Cuts at: 2580 2580
Size: 5980
AflII C'TTAA_G
Cuts at: 820 1017 2491 820
Size: 197 1474 4309
Fragments arranged by size:
4309 1474 197
AflIII A'CryG_T
Cuts at: 1203 4879 5856 1203
Size: 3676 977 1327
Fragments arranged by size:
3676 1327 977
AgeI A'CCGG_T
Cuts at: 4811 4811
Size: 5980
AhdI GACnn_n'nnGTC
Cuts at: 4709 4709
Size: 5980
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5980
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2411 1184
Size: 1227 4753
AlwNI CAG_nnn'CTG
Cuts at: 2516 3723 5295 2516
Size: 1207 1572 3201
Fragments arranged by size:
3201 1572 1207
ApaLI G'TGCA_C
Cuts at: 2619 4037 5193 2619
Size: 1418 1156 3406
Fragments arranged by size:
3406 1418 1156
ApoI r'AATT_y
Cuts at: 1493 2200 2447 2696 2807 2871 3194 3289 3515
Size: 707 247 249 111 64 323 95 226
Cuts at: 3515 3667 5776 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 707 323 249 247 226 152
111 95 64
AseI AT'TA_AT
Cuts at: 160 1032 4534 160
Size: 872 3502 1606
Fragments arranged by size:
3502 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
AvaI C'yCGr_G
Cuts at: 1870 1943 3076 3183 3305 1870
Size: 73 1133 107 122 4545
Fragments arranged by size:
4545 1133 122 107 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2941 2974 2941
Size: 33 5947
BamHI G'GATC_C
Cuts at: 3310 3677 3310
Size: 367 5613
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3301 4756 618
Size: 325 217 627 1514 1455 1842
Fragments arranged by size:
1842 1514 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3299 1391
Size: 1908 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2250 2344 2429 2676 928
Size: 971 351 94 85 247 4232
Fragments arranged by size:
4232 971 351 247 94 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3164 3198 4171 4205 1903
Size: 34 1227 34 973 34 3678
Fragments arranged by size:
3678 1227 973 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3872 5082 3872
Size: 1210 4770
BclI T'GATC_A
Cuts at: 1548 2278 1548
Size: 730 5250
BfrBI ATG'CAT
Cuts at: 2064 2463 3011 2064
Size: 399 548 5033
Fragments arranged by size:
5033 548 399
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4591 136
Size: 107 122 71 631 605 2919 1525
Fragments arranged by size:
2919 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5975 5975
Size: 5980
BlpI GC'TnA_GC
Cuts at: 3397 3397
Size: 5980
Bme1580I G_kGCm'C
Cuts at: 1790 2623 4041 5197 1790
Size: 833 1418 1156 2573
Fragments arranged by size:
2573 1418 1156 833
BmgBI CAC'GTC
Cuts at: 1318 2784 1318
Size: 1466 4514
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4669 442
Size: 416 53 83 399 24 2294 958 1753
Fragments arranged by size:
2294 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2471 1056
Size: 1415 4565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2736 3018 4640 1239
Size: 1497 282 1622 2579
Fragments arranged by size:
2579 1622 1497 282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2193 4105 4970 5268 2193
Size: 1912 865 298 2905
Fragments arranged by size:
2905 1912 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2752 2994 4643 882
Size: 617 1253 242 1649 2219
Fragments arranged by size:
2219 1649 1253 617 242
BsaAI yAC'GTr
Cuts at: 493 1204 2129 3771 493
Size: 711 925 1642 2702
Fragments arranged by size:
2702 1642 925 711
BsaBI GATnn'nnATC
Cuts at: 5974 5974
Size: 5980
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3787 4169 4804 275
Size: 53 83 186 936 2254 382 635 1451
Fragments arranged by size:
2254 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4413 4811 5085 5232 5643 4413
Size: 398 274 147 411 4750
Fragments arranged by size:
4750 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3264 3294 1178
Size: 30 165 30 1861 30 3864
Fragments arranged by size:
3864 1861 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5980
BseYI C'CCAG_C
Cuts at: 5183 5679 5183
Size: 496 5484
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2677 1163
Size: 573 941 4466
Fragments arranged by size:
4466 941 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3430 4191 5219 1540
Size: 275 186 1429 761 1028 2301
Fragments arranged by size:
2301 1429 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2537 2623 4041 4126 5197 2537
Size: 86 1418 85 1071 3320
Fragments arranged by size:
3320 1418 1071 86 85
BsmI GAATG_Cn'
Cuts at: 3491 3584 3491
Size: 93 5887
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2537 2623 3299 4041 4126 5197 1391
Size: 399 747 86 676 742 85 1071 2174
Fragments arranged by size:
2174 1071 747 742 676 399 86 85
BspEI T'CCGG_A
Cuts at: 5643 5643
Size: 5980
BspHI T'CATG_A
Cuts at: 3869 5599 3869
Size: 1730 4250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3335 844
Size: 2491 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 1394
Size: 429 2044 3507
Fragments arranged by size:
3507 2044 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4475 4649 65
Size: 1183 3227 174 1396
Fragments arranged by size:
3227 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4624 4811 1700
Size: 110 2814 187 2869
Fragments arranged by size:
2869 2814 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5980
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5980
BssSI C'ACGA_G
Cuts at: 4040 5052 4040
Size: 1012 4968
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5980
BstBI TT'CG_AA
Cuts at: 4784 4784
Size: 5980
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5980
BstYI r'GATC_y
Cuts at: 1972 2434 3310 3677 4062 4079 5531 5617 5629
Size: 462 876 367 385 17 1452 86 12
Cuts at: 5629 5975 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 876 462 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2592 2592
Size: 5980
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5241
Fragments arranged by size:
5241 683 56
BtsI GCAGTG_nn'
Cuts at: 2514 2799 3496 4290 4310 2514
Size: 285 697 794 20 4184
Fragments arranged by size:
4184 794 697 285 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5980
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5945
DraI TTT'AAA
Cuts at: 3284 3284
Size: 5980
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4987 809
Size: 507 3671 1802
Fragments arranged by size:
3671 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3427 4316 5945
Size: 54 1425 50 275 18 1597 889 1629
Cuts at: 5945 8
Size: 43
Fragments arranged by size:
1629 1597 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3427 1537
Size: 275 1615 4090
Fragments arranged by size:
4090 1615 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3910 1353
Size: 2557 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4567 4941 5087 387
Size: 1064 138 2978 374 146 1280
Fragments arranged by size:
2978 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2355 2736 2880 3018 4043 4640 5427
Size: 393 723 381 144 138 1025 597 787
Cuts at: 5427 5695 1239
Size: 268 1524
Fragments arranged by size:
1524 1025 787 723 597 393 381 268
144 138
EcoRI G'AATT_C
Cuts at: 2200 3194 3289 3667 2200
Size: 994 95 378 4513
Fragments arranged by size:
4513 994 378 95
FspI TGC'GCA
Cuts at: 4486 4486
Size: 5980
HaeII r_GCGC'y
Cuts at: 1678 2582 5127 1678
Size: 904 2545 2531
Fragments arranged by size:
2545 2531 904
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2263 2295 2714 2746 2751
Size: 32 135 32 691 32 419 32 5
Cuts at: 2751 2783 3125 3157 4621 4653 4695 4727 1373
Size: 32 342 32 1464 32 42 32 2626
Fragments arranged by size:
2626 1464 691 419 342 135 42 32
32 32 32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3324 3570 669
Size: 988 281 1386 246 3079
Fragments arranged by size:
3079 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5980
HpaI GTT'AAC
Cuts at: 3570 3570
Size: 5980
KpnI G_GTAC'C
Cuts at: 3305 3305
Size: 5980
MfeI C'AATT_G
Cuts at: 3579 3579
Size: 5980
MluI A'CGCG_T
Cuts at: 5856 5856
Size: 5980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5094 5278 1305
Size: 3789 184 2007
Fragments arranged by size:
3789 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4158
Fragments arranged by size:
4158 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3267 3938 4297 4456 518
Size: 648 2101 671 359 159 2042
Fragments arranged by size:
2101 2042 671 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2146 3396 4073 5221 5466 1606
Size: 540 1250 677 1148 245 2120
Fragments arranged by size:
2120 1250 1148 677 540 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4489
Fragments arranged by size:
4489 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5980
NheI G'CTAG_C
Cuts at: 1052 2467 1052
Size: 1415 4565
NotI GC'GGCC_GC
Cuts at: 3427 3427
Size: 5980
NsiI A_TGCA'T
Cuts at: 2066 2465 3013 2066
Size: 399 548 5033
Fragments arranged by size:
5033 548 399
NspI r_CATG'y
Cuts at: 1132 1229 2064 2467 3338 4883 1132
Size: 97 835 403 871 1545 2229
Fragments arranged by size:
2229 1545 871 835 403 97
PasI CC'CwG_GG
Cuts at: 1348 2834 1348
Size: 1486 4494
PciI A'CATG_T
Cuts at: 4879 4879
Size: 5980
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5980
PmeI GTTT'AAAC
Cuts at: 3284 3284
Size: 5980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 5562 5594 4049
Size: 32 1481 32 4435
Fragments arranged by size:
4435 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5980
PsiI TTA'TAA
Cuts at: 3550 3550
Size: 5980
PspXI vC'TCGA_Gb
Cuts at: 3183 3183
Size: 5980
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3162 3194 3162
Size: 32 5948
PstI C_TGCA'G
Cuts at: 830 2083 3332 830
Size: 1253 1249 3478
Fragments arranged by size:
3478 1253 1249
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5980
PvuII CAG'CTG
Cuts at: 2146 2146
Size: 5980
SalI G'TCGA_C
Cuts at: 1936 3322 1936
Size: 1386 4594
SbfI CC_TGCA'GG
Cuts at: 3332 3332
Size: 5980
ScaI AGT'ACT
Cuts at: 4228 5940 4228
Size: 1712 4268
SfcI C'TryA_G
Cuts at: 826 1045 2079 3328 4463 5144 5335 826
Size: 219 1034 1249 1135 681 191 1471
Fragments arranged by size:
1471 1249 1135 1034 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5980
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5980
SmaI CCC'GGG
Cuts at: 1872 3078 3307 1872
Size: 1206 229 4545
Fragments arranged by size:
4545 1206 229
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2208 2491 3183 4084 4985 5247
Size: 197 926 265 283 692 901 901 262
Cuts at: 5247 820
Size: 1553
Fragments arranged by size:
1553 926 901 901 692 283 265 262
197
SnaBI TAC'GTA
Cuts at: 493 3771 493
Size: 3278 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5980
SphI G_CATG'C
Cuts at: 1132 2064 2467 3338 1132
Size: 932 403 871 3774
Fragments arranged by size:
3774 932 871 403
SspI AAT'ATT
Cuts at: 5 52 3904 5
Size: 47 3852 2081
Fragments arranged by size:
3852 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5980
StyI C'CwwG_G
Cuts at: 513 1065 2004 3419 513
Size: 552 939 1415 3074
Fragments arranged by size:
3074 1415 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4007 4024 4177 4362 4807 325
Size: 1255 2427 17 153 185 445 1498
Fragments arranged by size:
2427 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4226 5938 96
Size: 274 80 33 51 1443 2249 1712 138
Fragments arranged by size:
2249 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5940 5972 5940
Size: 32 5948
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5980
XbaI T'CTAG_A
Cuts at: 3316 3316
Size: 5980
XhoI C'TCGA_G
Cuts at: 1943 3183 1943
Size: 1240 4740
XmaI C'CCGG_G
Cuts at: 1870 3076 3305 1870
Size: 1206 229 4545
Fragments arranged by size:
4545 1206 229
XmnI GAAnn'nnTTC
Cuts at: 2426 2448 4109 2426
Size: 22 1661 4297
Fragments arranged by size:
4297 1661 22
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3788 276
Size: 53 83 186 936 2254 2468
Fragments arranged by size:
2468 2254 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI