Restriction map for
pFN21AB6666
(Circular) (Six-base) MAPSORT of: pFN21AB6666.seq Check: 3158 from: 1 to: 6064
pFN21AB6666 6064 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2171 2190 2622 3407 1937
Size: 234 19 432 785 4594
Fragments arranged by size:
4594 785 432 234 19
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2910 4127 5511 5779 1632
Size: 1278 1217 1384 268 1917
Fragments arranged by size:
1917 1384 1278 1217 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 2072 4963 5940 1203
Size: 869 2891 977 1327
Fragments arranged by size:
2891 1327 977 869
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 4793 4793
Size: 6064
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 3024 3807 5379 3024
Size: 783 1572 3709
Fragments arranged by size:
3709 1572 783
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 2029 2756 3373 3599 3751 5860 1493
Size: 536 727 617 226 152 2109 1697
Fragments arranged by size:
2109 1697 727 617 536 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 3389 1870
Size: 73 1446 4545
Fragments arranged by size:
4545 1446 73
BamHI G'GATC_C
Cuts at: 3394 3761 3394
Size: 367 5697
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3385 4840 618
Size: 325 217 627 1598 1455 1842
Fragments arranged by size:
1842 1598 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3383 1391
Size: 1992 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2220 2841 928
Size: 971 321 621 4151
Fragments arranged by size:
4151 971 621 321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4255 4289 1903
Size: 34 2318 34 3678
Fragments arranged by size:
3678 2318 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3956 5166 3956
Size: 1210 4854
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6064
BfrBI ATG'CAT
Cuts at: 2816 2816
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4675 136
Size: 107 122 71 631 605 3003 1525
Fragments arranged by size:
3003 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 3481 3481
Size: 6064
Bme1580I G_kGCm'C
Cuts at: 1790 4125 5281 1790
Size: 2335 1156 2573
Fragments arranged by size:
2573 2335 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2538 3795 4753
Size: 416 53 83 399 24 1121 1257 958
Cuts at: 4753 442
Size: 1753
Fragments arranged by size:
1753 1257 1121 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2961 4724 1239
Size: 1722 1763 2579
Fragments arranged by size:
2579 1763 1722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2380 4189 5054 5352 2380
Size: 1809 865 298 3092
Fragments arranged by size:
3092 1809 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4727 882
Size: 617 3228 2219
Fragments arranged by size:
3228 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3855 493
Size: 711 2651 2702
Fragments arranged by size:
2702 2651 711
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3871 4253 4888 275
Size: 53 83 186 936 2338 382 635 1451
Fragments arranged by size:
2338 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4497 4895 5169 5316 5727 4497
Size: 398 274 147 411 4834
Fragments arranged by size:
4834 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5839
Fragments arranged by size:
5839 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6064
BseYI C'CCAG_C
Cuts at: 2469 2764 5267 5763 2469
Size: 295 2503 496 2770
Fragments arranged by size:
2770 2503 496 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3318 4125 4210 5281 3318
Size: 807 85 1071 4101
Fragments arranged by size:
4101 1071 807 85
BsmI GAATG_Cn'
Cuts at: 3575 3668 3575
Size: 93 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3318 3383 4125 4210 5281 1391
Size: 399 1528 65 742 85 1071 2174
Fragments arranged by size:
2174 1528 1071 742 399 85 65
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 3953 5683 3953
Size: 1730 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3419 844
Size: 2575 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2574 3951 1394
Size: 429 751 1377 3507
Fragments arranged by size:
3507 1377 751 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2154 2547 4559 4733 65
Size: 1183 906 393 2012 174 1396
Fragments arranged by size:
2012 1396 1183 906 393 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4708 4895 1700
Size: 110 2898 187 2869
Fragments arranged by size:
2898 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 4124 5136 4124
Size: 1012 5052
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2087 2867 794
Size: 1293 780 3991
Fragments arranged by size:
3991 1293 780
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstXI CCAn_nnnn'nTGG
Cuts at: 3116 3116
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 2197 3074 3394 3761 4146 4163 5615 5701
Size: 225 877 320 367 385 17 1452 86
Cuts at: 5701 5713 6059 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 877 385 367 346 320 225
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3351 3351
Size: 6064
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5512
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2985 487
Size: 683 56 1759 3566
Fragments arranged by size:
3566 1759 683 56
BtsI GCAGTG_nn'
Cuts at: 2689 3580 4374 4394 2689
Size: 891 794 20 4359
Fragments arranged by size:
4359 891 794 20
ClaI AT'CG_AT
Cuts at: 1554 2378 2634 1554
Size: 824 256 4984
Fragments arranged by size:
4984 824 256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3106 3511 4400
Size: 54 1425 50 275 18 1276 405 889
Cuts at: 4400 6029 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1276 889 405 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2071 2663 3994 1353
Size: 718 592 1331 3423
Fragments arranged by size:
3423 1331 718 592
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4651 5025 5171 387
Size: 1064 138 3062 374 146 1280
Fragments arranged by size:
3062 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2910 2961 4127 4724 5511 5779 1239
Size: 393 1278 51 1166 597 787 268 1524
Fragments arranged by size:
1524 1278 1166 787 597 393 268 51
EcoO109I rG'GnC_Cy
Cuts at: 2885 2885
Size: 6064
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
EcoRV GAT'ATC
Cuts at: 2524 2524
Size: 6064
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 5211 1678
Size: 3533 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2153 2185 2189 2221 2285
Size: 32 135 32 581 32 4 32 64
Cuts at: 2285 2317 4705 4737 4779 4811 1373
Size: 32 2388 32 42 32 2626
Fragments arranged by size:
2626 2388 581 135 64 42 32 32
32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2449 3408 3654 669
Size: 988 281 511 959 246 3079
Fragments arranged by size:
3079 988 959 511 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 2449 3654 2449
Size: 1205 4859
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 3261 3663 3261
Size: 402 5662
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2165 2299 5178 5362 1305
Size: 860 134 2879 184 2007
Fragments arranged by size:
2879 2007 860 184 134
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3108 10
Size: 54 1425 343 1276 2966
Fragments arranged by size:
2966 1425 1276 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3114 4022 4381 4540 518
Size: 648 1948 908 359 159 2042
Fragments arranged by size:
2042 1948 908 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2260 3480 4157 5305 5550 1606
Size: 654 1220 677 1148 245 2120
Fragments arranged by size:
2120 1220 1148 677 654 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5512
NdeI CA'TA_TG
Cuts at: 387 2083 2812 2914 387
Size: 1696 729 102 3537
Fragments arranged by size:
3537 1696 729 102
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NsiI A_TGCA'T
Cuts at: 2818 2818
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2076 3013 3422 4967 1132
Size: 97 847 937 409 1545 2229
Fragments arranged by size:
2229 1545 937 847 409 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 2072 4963 2072
Size: 2891 3173
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5162
PfoI T'CCnGG_A
Cuts at: 2339 2339
Size: 6064
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2885 2885
Size: 6064
PshAI GACnn'nnGTC
Cuts at: 1613 2734 1613
Size: 1121 4943
PsiI TTA'TAA
Cuts at: 2286 3634 2286
Size: 1348 4716
PstI C_TGCA'G
Cuts at: 830 2863 3416 830
Size: 2033 553 3478
Fragments arranged by size:
3478 2033 553
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
PvuII CAG'CTG
Cuts at: 2260 2260
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 2857 4312 6024 2857
Size: 1455 1712 2897
Fragments arranged by size:
2897 1712 1455
SfcI C'TryA_G
Cuts at: 826 1045 2201 2859 3412 4547 5228 5419 826
Size: 219 1156 658 553 1135 681 191 1471
Fragments arranged by size:
1471 1156 1135 681 658 553 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2395 4168 5069 5331 820
Size: 197 926 452 1773 901 262 1553
Fragments arranged by size:
1773 1553 926 901 452 262 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 3013 3422 1132
Size: 1881 409 3774
Fragments arranged by size:
3774 1881 409
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6064
StyI C'CwwG_G
Cuts at: 513 1065 3130 3503 513
Size: 552 2065 373 3074
Fragments arranged by size:
3074 2065 552 373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4091 4108 4261 4446 4891 325
Size: 1255 2511 17 153 185 445 1498
Fragments arranged by size:
2511 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2855 4310 6022
Size: 274 80 33 51 1443 878 1455 1712
Cuts at: 6022 96
Size: 138
Fragments arranged by size:
1712 1455 1443 878 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2272 2304 2643 2675 6024 6056 2272
Size: 32 339 32 3349 32 2280
Fragments arranged by size:
3349 2280 339 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6064
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2525 2878 2525
Size: 353 5711
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6064
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 4193 4193
Size: 6064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI