Restriction map for
pFN21AB6662
(Circular) (Six-base) MAPSORT of: pFN21AB6662.seq Check: 7773 from: 1 to: 6058
pFN21AB6662 6058 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3868 278
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3401 1937
Size: 1464 4594
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2162 2252 3351 4121 5505 5773 1632
Size: 530 90 1099 770 1384 268 1917
Fragments arranged by size:
1917 1384 1099 770 530 268 90
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 4957 5934 1203
Size: 3754 977 1327
Fragments arranged by size:
3754 1327 977
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 3161 4787 3161
Size: 1626 4432
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6058
AlwNI CAG_nnn'CTG
Cuts at: 3801 5373 3801
Size: 1572 4486
ApaLI G'TGCA_C
Cuts at: 3200 4115 5271 3200
Size: 915 1156 3987
Fragments arranged by size:
3987 1156 915
ApoI r'AATT_y
Cuts at: 1493 2069 2431 2986 3294 3367 3593 3745 5854
Size: 576 362 555 308 73 226 152 2109
Cuts at: 5854 1493
Size: 1697
Fragments arranged by size:
2109 1697 576 555 362 308 226 152
73
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 2836 3383 1870
Size: 73 893 547 4545
Fragments arranged by size:
4545 893 547 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3172 3205 3172
Size: 33 6025
BamHI G'GATC_C
Cuts at: 3388 3755 3388
Size: 367 5691
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3379 4834 618
Size: 325 217 627 1592 1455 1842
Fragments arranged by size:
1842 1592 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3377 1391
Size: 1986 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5087
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4249 4283 1903
Size: 34 2312 34 3678
Fragments arranged by size:
3678 2312 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3173 3950 5160 3173
Size: 777 1210 4071
Fragments arranged by size:
4071 1210 777
BclI T'GATC_A
Cuts at: 1548 2449 1548
Size: 901 5157
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4669 136
Size: 107 122 71 631 605 2997 1525
Fragments arranged by size:
2997 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6053 6053
Size: 6058
BlpI GC'TnA_GC
Cuts at: 3475 3475
Size: 6058
Bme1580I G_kGCm'C
Cuts at: 1790 3204 4119 5275 1790
Size: 1414 915 1156 2573
Fragments arranged by size:
2573 1414 1156 915
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3789 4747 442
Size: 416 53 83 399 24 2372 958 1753
Fragments arranged by size:
2372 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3093 3093
Size: 6058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2886 3360 4718 1239
Size: 1647 474 1358 2579
Fragments arranged by size:
2579 1647 1358 474
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2818 4183 5048 5346 2818
Size: 1365 865 298 3530
Fragments arranged by size:
3530 1365 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4721 882
Size: 617 3222 2219
Fragments arranged by size:
3222 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3849 493
Size: 711 2645 2702
Fragments arranged by size:
2702 2645 711
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3865 4247 4882 275
Size: 53 83 186 936 2332 382 635 1451
Fragments arranged by size:
2332 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4491 4889 5163 5310 5721 4491
Size: 398 274 147 411 4828
Fragments arranged by size:
4828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3022 3052 1178
Size: 30 165 30 1619 30 4184
Fragments arranged by size:
4184 1619 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 2666 1186
Size: 982 498 4578
Fragments arranged by size:
4578 982 498
BseYI C'CCAG_C
Cuts at: 3138 5261 5757 3138
Size: 2123 496 3439
Fragments arranged by size:
3439 2123 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2142 1163
Size: 573 406 5079
Fragments arranged by size:
5079 573 406
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3136 3508 4269 5297 1540
Size: 275 186 1135 372 761 1028 2301
Fragments arranged by size:
2301 1135 1028 761 372 275 186
BsiHKAI G_wGCw'C
Cuts at: 3204 4119 4204 5275 3204
Size: 915 85 1071 3987
Fragments arranged by size:
3987 1071 915 85
BsmI GAATG_Cn'
Cuts at: 2521 3569 3662 2521
Size: 1048 93 4917
Fragments arranged by size:
4917 1048 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3204 3377 4119 4204 5275 1391
Size: 399 1414 173 742 85 1071 2174
Fragments arranged by size:
2174 1414 1071 742 399 173 85
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 3947 5677 3947
Size: 1730 4328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3413 844
Size: 2569 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3072 3945 1394
Size: 429 1249 873 3507
Fragments arranged by size:
3507 1249 873 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2561 3308 4553 4727 65
Size: 1183 1313 747 1245 174 1396
Fragments arranged by size:
1396 1313 1245 1183 747 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4702 4889 1700
Size: 110 2892 187 2869
Fragments arranged by size:
2892 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 2180 2880 3080 4118 5130 2180
Size: 700 200 1038 1012 3108
Fragments arranged by size:
3108 1038 1012 700 200
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2165 794
Size: 1371 4687
BstBI TT'CG_AA
Cuts at: 4862 4862
Size: 6058
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstXI CCAn_nnnn'nTGG
Cuts at: 2410 2510 2410
Size: 100 5958
BstYI r'GATC_y
Cuts at: 1972 3388 3755 4140 4157 5609 5695 5707 6053
Size: 1416 367 385 17 1452 86 12 346
Cuts at: 6053 1972
Size: 1977
Fragments arranged by size:
1977 1452 1416 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4567
Fragments arranged by size:
4567 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5319
Fragments arranged by size:
5319 683 56
BtsI GCAGTG_nn'
Cuts at: 2018 3574 4368 4388 2018
Size: 1556 794 20 3688
Fragments arranged by size:
3688 1556 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2548 2583 529
Size: 35 1984 35 4004
Fragments arranged by size:
4004 1984 35 35
DraI TTT'AAA
Cuts at: 3362 3362
Size: 6058
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5065 809
Size: 507 3749 1802
Fragments arranged by size:
3749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3109 3505 4394
Size: 54 1425 50 275 18 1279 396 889
Cuts at: 4394 6023 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1279 889 396 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3505 1537
Size: 275 1693 4090
Fragments arranged by size:
4090 1693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2313 2366 3988 1353
Size: 960 53 1622 3423
Fragments arranged by size:
3423 1622 960 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4645 5019 5165 387
Size: 1064 138 3056 374 146 1280
Fragments arranged by size:
3056 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2252 2886 3351 3360 4121 4718
Size: 393 530 90 634 465 9 761 597
Cuts at: 4718 5505 5773 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 761 634 597 530 465 393
268 90 9
EcoO109I rG'GnC_Cy
Cuts at: 2822 2858 2903 2822
Size: 36 45 5977
Fragments arranged by size:
5977 45 36
EcoRI G'AATT_C
Cuts at: 3367 3745 3367
Size: 378 5680
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 5205 1678
Size: 3527 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2066 2071 2098 2434
Size: 32 135 32 467 27 5 27 336
Cuts at: 2434 2466 2516 2548 3147 3179 4699 4731 4773
Size: 32 50 32 599 32 1520 32 42
Cuts at: 4773 4805 1373
Size: 32 2626
Fragments arranged by size:
2626 1520 599 467 336 135 50 42
32 32 32 32 32 32 32 27
27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3402 3648 669
Size: 988 281 1464 246 3079
Fragments arranged by size:
3079 1464 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6058
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 2934 3657 2934
Size: 723 5335
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3133 5172 5356 1305
Size: 1828 2039 184 2007
Fragments arranged by size:
2039 2007 1828 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4236
Fragments arranged by size:
4236 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2514 4016 4375 4534 518
Size: 648 1348 1502 359 159 2042
Fragments arranged by size:
2042 1502 1348 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3108 3474 4151 5299 5544 1606
Size: 1502 366 677 1148 245 2120
Fragments arranged by size:
2120 1502 1148 677 366 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4567
Fragments arranged by size:
4567 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6058
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6058
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NspI r_CATG'y
Cuts at: 1132 1229 3416 4961 1132
Size: 97 2187 1545 2229
Fragments arranged by size:
2229 2187 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6058
PciI A'CATG_T
Cuts at: 4957 4957
Size: 6058
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6058
PfoI T'CCnGG_A
Cuts at: 2827 2944 2827
Size: 117 5941
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 5640 5672 4127
Size: 32 1481 32 4513
Fragments arranged by size:
4513 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2822 2858 2903 2822
Size: 36 45 5977
Fragments arranged by size:
5977 45 36
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6058
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PstI C_TGCA'G
Cuts at: 830 2478 3410 830
Size: 1648 932 3478
Fragments arranged by size:
3478 1648 932
PvuI CG_AT'CG
Cuts at: 2001 3136 2001
Size: 1135 4923
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 2742 4306 6018 2742
Size: 1564 1712 2782
Fragments arranged by size:
2782 1712 1564
SfcI C'TryA_G
Cuts at: 826 1045 2474 3166 3406 4541 5222 5413 826
Size: 219 1429 692 240 1135 681 191 1471
Fragments arranged by size:
1471 1429 1135 692 681 240 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 2838 3385 1872
Size: 966 547 4545
Fragments arranged by size:
4545 966 547
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2797 4162 5063 5325 820
Size: 197 926 854 1365 901 262 1553
Fragments arranged by size:
1553 1365 926 901 854 262 197
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3416 1132
Size: 2284 3774
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6058
StyI C'CwwG_G
Cuts at: 513 1065 2004 3497 513
Size: 552 939 1493 3074
Fragments arranged by size:
3074 1493 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4085 4102 4255 4440 4885 325
Size: 1255 2505 17 153 185 445 1498
Fragments arranged by size:
2505 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2740 4304 6016
Size: 274 80 33 51 1443 763 1564 1712
Cuts at: 6016 96
Size: 138
Fragments arranged by size:
1712 1564 1443 763 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3022 3054 6018 6050 3022
Size: 32 2964 32 3030
Fragments arranged by size:
3030 2964 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 3394 3394
Size: 6058
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6058
XmaI C'CCGG_G
Cuts at: 1870 2836 3383 1870
Size: 966 547 4545
Fragments arranged by size:
4545 966 547
XmnI GAAnn'nnTTC
Cuts at: 2147 2618 4187 2147
Size: 471 1569 4018
Fragments arranged by size:
4018 1569 471
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3866 276
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI