Restriction map for
pFN21AB6661
(Circular) (Six-base) MAPSORT of: pFN21AB6661.seq Check: 8895 from: 1 to: 5977
pFN21AB6661 5977 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2252 3787 278
Size: 53 83 186 936 716 1535 2468
Fragments arranged by size:
2468 1535 936 716 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3201 4040 5424 5692 1632
Size: 1569 839 1384 268 1917
Fragments arranged by size:
1917 1569 1384 839 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 4876 5853 1203
Size: 3673 977 1327
Fragments arranged by size:
3673 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2543 1184
Size: 1359 4618
AlwNI CAG_nnn'CTG
Cuts at: 2209 2451 2717 3720 5292 2209
Size: 242 266 1003 1572 2894
Fragments arranged by size:
2894 1572 1003 266 242
ApaI G_GGCC'C
Cuts at: 2234 2234
Size: 5977
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 2353 2391 3207 3286 3512 3664 5773 1493
Size: 860 38 816 79 226 152 2109 1697
Fragments arranged by size:
2109 1697 860 816 226 152 79 38
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 2039 2234 3302 1870
Size: 73 96 195 1068 4545
Fragments arranged by size:
4545 1068 195 96 73
AvrII C'CTAG_G
Cuts at: 2080 2080
Size: 5977
BamHI G'GATC_C
Cuts at: 2430 3307 3674 2430
Size: 877 367 4733
Fragments arranged by size:
4733 877 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3298 4753 618
Size: 325 217 627 1511 1455 1842
Fragments arranged by size:
1842 1511 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2181 2234 3085 3296 1391
Size: 790 53 851 211 4072
Fragments arranged by size:
4072 851 790 211 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2749 928
Size: 971 850 4156
Fragments arranged by size:
4156 971 850
BbvCI CC'TCA_GC
Cuts at: 3075 3075
Size: 5977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4168 4202 1903
Size: 34 2231 34 3678
Fragments arranged by size:
3678 2231 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 2787 1548
Size: 1239 4738
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4588 136
Size: 107 122 71 631 605 2916 1525
Fragments arranged by size:
2916 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 2129 3394 2129
Size: 1265 4712
Bme1580I G_kGCm'C
Cuts at: 1790 2234 4038 5194 1790
Size: 444 1804 1156 2573
Fragments arranged by size:
2573 1804 1156 444
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3708 4666 442
Size: 416 53 83 399 24 2291 958 1753
Fragments arranged by size:
2291 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4637 1239
Size: 3398 2579
Bpu10I CC'TnA_GC
Cuts at: 3075 3167 3075
Size: 92 5885
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2894 3120 4102 4967 5265 2894
Size: 226 982 865 298 3606
Fragments arranged by size:
3606 982 865 298 226
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3264 4640 882
Size: 617 1765 1376 2219
Fragments arranged by size:
2219 1765 1376 617
BsaAI yAC'GTr
Cuts at: 493 1204 3768 493
Size: 711 2564 2702
Fragments arranged by size:
2702 2564 711
BsaBI GATnn'nnATC
Cuts at: 2429 2487 2792 5971 2429
Size: 58 305 3179 2435
Fragments arranged by size:
3179 2435 305 58
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2249 3784 4166 4801
Size: 53 83 186 936 716 1535 382 635
Cuts at: 4801 275
Size: 1451
Fragments arranged by size:
1535 1451 936 716 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2325 2499 4410 4808 5082 5229 5640 2325
Size: 174 1911 398 274 147 411 2662
Fragments arranged by size:
2662 1911 411 398 274 174 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3032 3062 3261 3291 1178
Size: 30 165 30 1629 30 199 30 3864
Fragments arranged by size:
3864 1629 199 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2785 1186
Size: 1599 4378
BseYI C'CCAG_C
Cuts at: 2191 5180 5676 2191
Size: 2989 496 2492
Fragments arranged by size:
2989 2492 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5404
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2218 3427 4188 5216 1540
Size: 275 186 217 1209 761 1028 2301
Fragments arranged by size:
2301 1209 1028 761 275 217 186
BsiHKAI G_wGCw'C
Cuts at: 2522 4038 4123 5194 2522
Size: 1516 85 1071 3305
Fragments arranged by size:
3305 1516 1071 85
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
BsmBI CGTCTCn'nnnn_
Cuts at: 2374 2374
Size: 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2181 2234 2522 3085 3296 4038 4123
Size: 399 391 53 288 563 211 742 85
Cuts at: 4123 5194 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 563 399 391 288 211
85 53
BspEI T'CCGG_A
Cuts at: 2499 5640 2499
Size: 3141 2836
BspHI T'CATG_A
Cuts at: 3866 5596 3866
Size: 1730 4247
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2670 3332 844
Size: 1826 662 3489
Fragments arranged by size:
3489 1826 662
BsrBI CCG'CTC
Cuts at: 1394 1823 2620 3864 1394
Size: 429 797 1244 3507
Fragments arranged by size:
3507 1244 797 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4472 4646 65
Size: 1183 3224 174 1396
Fragments arranged by size:
3224 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2019 2220 2257 4621 4808 1700
Size: 110 209 201 37 2364 187 2869
Fragments arranged by size:
2869 2364 209 201 187 110 37
BsrGI T'GTAC_A
Cuts at: 96 2442 96
Size: 2346 3631
BssHII G'CGCG_C
Cuts at: 1925 2278 1925
Size: 353 5624
BssSI C'ACGA_G
Cuts at: 2123 2681 4037 5049 2123
Size: 558 1356 1012 3051
Fragments arranged by size:
3051 1356 1012 558
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2125 2209 2483 794
Size: 1331 84 274 4288
Fragments arranged by size:
4288 1331 274 84
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 3245 1184
Size: 2061 3916
BstYI r'GATC_y
Cuts at: 1972 2430 2793 3042 3307 3674 4059 4076 5528
Size: 458 363 249 265 367 385 17 1452
Cuts at: 5528 5614 5626 5972 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 458 385 367 363 346 265
249 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2064 2141 2226 3265 513
Size: 552 999 77 85 1039 3225
Fragments arranged by size:
3225 1039 999 552 85 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2438 487
Size: 683 56 1212 4026
Fragments arranged by size:
4026 1212 683 56
BtsI GCAGTG_nn'
Cuts at: 2484 2727 3213 3493 4287 4307 2484
Size: 243 486 280 794 20 4154
Fragments arranged by size:
4154 794 486 280 243 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DraIII CAC_nnn'GTG
Cuts at: 2337 2337
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2215 2556 3424
Size: 54 1425 50 275 18 385 341 868
Cuts at: 3424 4313 5942 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 868 385 341 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2215 3424 1537
Size: 275 403 1209 4090
Fragments arranged by size:
4090 1209 403 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3907 1353
Size: 2554 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3201 4040 4637 5424 5692 1239
Size: 393 1569 839 597 787 268 1524
Fragments arranged by size:
1569 1524 839 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2027 2085 2231 2238 2551 2623 2027
Size: 58 146 7 313 72 5381
Fragments arranged by size:
5381 313 146 72 58 7
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
EcoRV GAT'ATC
Cuts at: 2890 2890
Size: 5977
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 2192 5124 1678
Size: 514 2932 2531
Fragments arranged by size:
2932 2531 514
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2354 2386 2772 2804 4618
Size: 32 135 32 782 32 386 32 1814
Cuts at: 4618 4650 4692 4724 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1814 782 386 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2362 3321 3567 669
Size: 988 281 424 959 246 3079
Fragments arranged by size:
3079 988 959 424 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5977
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3201 3576 3201
Size: 375 5602
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5091 5275 1305
Size: 3786 184 2007
Fragments arranged by size:
3786 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2558 10
Size: 54 1425 343 726 3429
Fragments arranged by size:
3429 1425 726 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3935 4294 4453 518
Size: 648 2769 359 159 2042
Fragments arranged by size:
2769 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 2172 2206 2228 2288 2437 3393 4070
Size: 537 29 34 22 60 149 956 677
Cuts at: 4070 5218 5463 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 956 677 537 245 149 60
34 29 22
NaeI GCC'GGC
Cuts at: 2021 2222 2259 2021
Size: 201 37 5739
Fragments arranged by size:
5739 201 37
NcoI C'CATG_G
Cuts at: 513 1065 2064 3265 513
Size: 552 999 1201 3225
Fragments arranged by size:
3225 1201 999 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NgoMIV G'CCGG_C
Cuts at: 2019 2220 2257 2019
Size: 201 37 5739
Fragments arranged by size:
5739 201 37
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 3175 3335 4880 1132
Size: 97 1946 160 1545 2229
Fragments arranged by size:
2229 1946 1545 160 97
PasI CC'CwG_GG
Cuts at: 1348 2968 1348
Size: 1620 4357
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2337 3266 1108
Size: 1229 929 3819
Fragments arranged by size:
3819 1229 929
PfoI T'CCnGG_A
Cuts at: 3122 3122
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2027 2085 2238 2027
Size: 58 153 5766
Fragments arranged by size:
5766 153 58
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PspOMI G'GGCC_C
Cuts at: 2230 2230
Size: 5977
PstI C_TGCA'G
Cuts at: 830 2205 2722 2926 3329 830
Size: 1375 517 204 403 3478
Fragments arranged by size:
3478 1375 517 403 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
PvuII CAG'CTG
Cuts at: 2206 2437 2206
Size: 231 5746
RsrII CG'GwC_CG
Cuts at: 2266 2266
Size: 5977
SacII CC_GC'GG
Cuts at: 2144 2229 2144
Size: 85 5892
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SanDI GG'GwC_CC
Cuts at: 2238 2238
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2201 2718 2922 3325 4460 5141 5332
Size: 219 1156 517 204 403 1135 681 191
Cuts at: 5332 826
Size: 1471
Fragments arranged by size:
1471 1156 1135 681 517 403 219 204
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 2236 3304 1872
Size: 364 1068 4545
Fragments arranged by size:
4545 1068 364
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2873 3135 4081 4982 5244 820
Size: 197 926 930 262 946 901 262 1553
Fragments arranged by size:
1553 946 930 926 901 262 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 3175 3335 1132
Size: 2043 160 3774
Fragments arranged by size:
3774 2043 160
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5977
StyI C'CwwG_G
Cuts at: 513 1065 2064 2080 3265 3416 513
Size: 552 999 16 1185 151 3074
Fragments arranged by size:
3074 1185 999 552 151 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2254 3102 3131 4004 4021 4174 4359
Size: 1255 674 848 29 873 17 153 185
Cuts at: 4359 4804 325
Size: 445 1498
Fragments arranged by size:
1498 1255 873 848 674 445 185 153
29 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2442 4223 5935
Size: 274 80 33 51 1443 465 1781 1712
Cuts at: 5935 96
Size: 138
Fragments arranged by size:
1781 1712 1443 465 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3032 3064 5937 5969 3032
Size: 32 2873 32 3040
Fragments arranged by size:
3040 2873 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2366 1316
Size: 1050 4927
XbaI T'CTAG_A
Cuts at: 3313 3313
Size: 5977
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 2234 3302 1870
Size: 364 1068 4545
Fragments arranged by size:
4545 1068 364
XmnI GAAnn'nnTTC
Cuts at: 4106 4106
Size: 5977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2250 3785 276
Size: 53 83 186 936 716 1535 2468
Fragments arranged by size:
2468 1535 936 716 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI SacI SapI SexAI SfiI SfoI SrfI SwaI
XcmI