Restriction map for
pFN21AB6660
(Circular) (Six-base) MAPSORT of: pFN21AB6660.seq Check: 7675 from: 1 to: 6058
pFN21AB6660 6058 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2087 2087
Size: 6058
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3868 278
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3401 1937
Size: 1464 4594
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2217 4121 5505 5773 1632
Size: 585 1904 1384 268 1917
Fragments arranged by size:
1917 1904 1384 585 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 4957 5934 1203
Size: 3754 977 1327
Fragments arranged by size:
3754 1327 977
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 2210 2932 4787 2210
Size: 722 1855 3481
Fragments arranged by size:
3481 1855 722
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2589 1184
Size: 1405 4653
AlwNI CAG_nnn'CTG
Cuts at: 3801 5373 3801
Size: 1572 4486
ApaI G_GGCC'C
Cuts at: 2477 2477
Size: 6058
ApaLI G'TGCA_C
Cuts at: 4115 5271 4115
Size: 1156 4902
ApoI r'AATT_y
Cuts at: 1493 2354 3074 3182 3350 3367 3593 3745 5854
Size: 861 720 108 168 17 226 152 2109
Cuts at: 5854 1493
Size: 1697
Fragments arranged by size:
2109 1697 861 720 226 168 152 108
17
AseI AT'TA_AT
Cuts at: 160 1032 2264 4612 160
Size: 872 1232 2348 1606
Fragments arranged by size:
2348 1606 1232 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 3383 1870
Size: 73 1440 4545
Fragments arranged by size:
4545 1440 73
BamHI G'GATC_C
Cuts at: 3388 3755 3388
Size: 367 5691
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3379 4834 618
Size: 325 217 627 1592 1455 1842
Fragments arranged by size:
1842 1592 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2477 3377 1391
Size: 1086 900 4072
Fragments arranged by size:
4072 1086 900
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2244 2300 2931 928
Size: 971 345 56 631 4055
Fragments arranged by size:
4055 971 631 345 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4249 4283 1903
Size: 34 50 34 2228 34 3678
Fragments arranged by size:
3678 2228 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3950 5160 3950
Size: 1210 4848
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6058
BfrBI ATG'CAT
Cuts at: 2141 2803 2141
Size: 662 5396
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4669 136
Size: 107 122 71 631 605 2997 1525
Fragments arranged by size:
2997 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6053 6053
Size: 6058
BlpI GC'TnA_GC
Cuts at: 3475 3475
Size: 6058
Bme1580I G_kGCm'C
Cuts at: 1790 2477 4119 5275 1790
Size: 687 1642 1156 2573
Fragments arranged by size:
2573 1642 1156 687
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3209 3789 4747
Size: 416 53 83 399 24 1792 580 958
Cuts at: 4747 442
Size: 1753
Fragments arranged by size:
1792 1753 958 580 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4718 1239
Size: 3479 2579
Bpu10I CC'TnA_GC
Cuts at: 2951 2951
Size: 6058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2217 2816 4183 5048 5346 2217
Size: 599 1367 865 298 2929
Fragments arranged by size:
2929 1367 865 599 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4721 882
Size: 617 3222 2219
Fragments arranged by size:
3222 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3849 493
Size: 711 2645 2702
Fragments arranged by size:
2702 2645 711
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3865 4247 4882 275
Size: 53 83 186 936 2332 382 635 1451
Fragments arranged by size:
2332 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4491 4889 5163 5310 5721 4491
Size: 398 274 147 411 4828
Fragments arranged by size:
4828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5833
Fragments arranged by size:
5833 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2816 1186
Size: 1630 4428
BseYI C'CCAG_C
Cuts at: 2470 3105 5261 5757 2470
Size: 635 2156 496 2771
Fragments arranged by size:
2771 2156 635 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2454 1163
Size: 573 718 4767
Fragments arranged by size:
4767 718 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3508 4269 5297 1540
Size: 275 186 1507 761 1028 2301
Fragments arranged by size:
2301 1507 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4119 4204 5275 4119
Size: 85 1071 4902
Fragments arranged by size:
4902 1071 85
BsmI GAATG_Cn'
Cuts at: 3569 3662 3569
Size: 93 5965
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2477 3377 4119 4204 5275 1391
Size: 399 687 900 742 85 1071 2174
Fragments arranged by size:
2174 1071 900 742 687 399 85
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 3947 5677 3947
Size: 1730 4328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2087 3413 844
Size: 1243 1326 3489
Fragments arranged by size:
3489 1326 1243
BsrBI CCG'CTC
Cuts at: 1394 1823 3945 1394
Size: 429 2122 3507
Fragments arranged by size:
3507 2122 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2161 4553 4727 65
Size: 1183 913 2392 174 1396
Fragments arranged by size:
2392 1396 1183 913 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4702 4889 1700
Size: 110 2892 187 2869
Fragments arranged by size:
2892 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 4118 5130 4118
Size: 1012 5046
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6058
BstBI TT'CG_AA
Cuts at: 3190 3264 4862 3190
Size: 74 1598 4386
Fragments arranged by size:
4386 1598 74
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstXI CCAn_nnnn'nTGG
Cuts at: 2168 2168
Size: 6058
BstYI r'GATC_y
Cuts at: 1972 3388 3755 4140 4157 5609 5695 5707 6053
Size: 1416 367 385 17 1452 86 12 346
Cuts at: 6053 1972
Size: 1977
Fragments arranged by size:
1977 1452 1416 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3032 3032
Size: 6058
BtgI C'CryG_G
Cuts at: 513 1065 2038 513
Size: 552 973 4533
Fragments arranged by size:
4533 973 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5319
Fragments arranged by size:
5319 683 56
BtsI GCAGTG_nn'
Cuts at: 2053 2173 3045 3574 4368 4388 2053
Size: 120 872 529 794 20 3723
Fragments arranged by size:
3723 872 794 529 120 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6023
DraI TTT'AAA
Cuts at: 3362 3362
Size: 6058
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5065 809
Size: 507 3749 1802
Fragments arranged by size:
3749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2101 2892 3505
Size: 54 1425 50 275 18 271 791 613
Cuts at: 3505 4394 6023 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 791 613 275 271 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3505 1537
Size: 275 1693 4090
Fragments arranged by size:
4090 1693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2487 2742 3988 1353
Size: 1134 255 1246 3423
Fragments arranged by size:
3423 1246 1134 255
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4645 5019 5165 387
Size: 1064 138 3056 374 146 1280
Fragments arranged by size:
3056 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 4121 4718 5505 5773 1239
Size: 393 585 1904 597 787 268 1524
Fragments arranged by size:
1904 1524 787 597 585 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2219 2219
Size: 6058
EcoO109I rG'GnC_Cy
Cuts at: 2474 2955 2474
Size: 481 5577
EcoRI G'AATT_C
Cuts at: 3182 3350 3367 3745 3182
Size: 168 17 378 5495
Fragments arranged by size:
5495 378 168 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2327 2327
Size: 6058
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 5205 1678
Size: 3527 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2196 2228 2918 2950 4699
Size: 32 135 32 624 32 690 32 1749
Cuts at: 4699 4731 4773 4805 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1749 690 624 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3402 3648 669
Size: 988 281 1464 246 3079
Fragments arranged by size:
3079 1464 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2792 2813 748
Size: 2044 21 3993
Fragments arranged by size:
3993 2044 21
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 2145 3657 2145
Size: 1512 4546
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3083 3325 5172 5356 1305
Size: 1778 242 1847 184 2007
Fragments arranged by size:
2007 1847 1778 242 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2103 2894 10
Size: 54 1425 343 271 791 3174
Fragments arranged by size:
3174 1425 791 343 271 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2112 2166 3123 4016 4375 4534 518
Size: 648 946 54 957 893 359 159 2042
Fragments arranged by size:
2042 957 946 893 648 359 159 54
MspA1I CmG'CkG
Cuts at: 1606 2040 2438 2470 3010 3474 4151 5299 5544
Size: 434 398 32 540 464 677 1148 245
Cuts at: 5544 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 540 464 434 398 245
32
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5506
NdeI CA'TA_TG
Cuts at: 387 2765 387
Size: 2378 3680
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6058
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NsiI A_TGCA'T
Cuts at: 2143 2805 2143
Size: 662 5396
NspI r_CATG'y
Cuts at: 1132 1229 3094 3416 4961 1132
Size: 97 1865 322 1545 2229
Fragments arranged by size:
2229 1865 1545 322 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6058
PciI A'CATG_T
Cuts at: 4957 4957
Size: 6058
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6058
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 5640 5672 4127
Size: 32 1481 32 4513
Fragments arranged by size:
4513 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6058
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PspOMI G'GGCC_C
Cuts at: 2473 2473
Size: 6058
PstI C_TGCA'G
Cuts at: 830 2443 3289 3410 830
Size: 1613 846 121 3478
Fragments arranged by size:
3478 1613 846 121
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
PvuII CAG'CTG
Cuts at: 2438 2470 2438
Size: 32 6026
SacII CC_GC'GG
Cuts at: 2041 2041
Size: 6058
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SfcI C'TryA_G
Cuts at: 826 1045 2439 3285 3406 4541 5222 5413 826
Size: 219 1394 846 121 1135 681 191 1471
Fragments arranged by size:
1471 1394 1135 846 681 219 191 121
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 3385 1872
Size: 1513 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2232 2795 4162 5063 5325 820
Size: 197 926 289 563 1367 901 262 1553
Fragments arranged by size:
1553 1367 926 901 563 289 262 197
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3416 1132
Size: 2284 3774
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6058
StyI C'CwwG_G
Cuts at: 513 1065 2958 3497 513
Size: 552 1893 539 3074
Fragments arranged by size:
3074 1893 552 539
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4085 4102 4255 4440 4885 325
Size: 1255 2505 17 153 185 445 1498
Fragments arranged by size:
2505 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 4304 6016
Size: 274 80 33 51 1443 270 2057 1712
Cuts at: 6016 96
Size: 138
Fragments arranged by size:
2057 1712 1443 274 270 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3201 3233 6018 6050 3201
Size: 32 2785 32 3209
Fragments arranged by size:
3209 2785 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 3394 3394
Size: 6058
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6058
XmaI C'CCGG_G
Cuts at: 1870 3383 1870
Size: 1513 4545
XmnI GAAnn'nnTTC
Cuts at: 2358 3186 4187 2358
Size: 828 1001 4229
Fragments arranged by size:
4229 1001 828
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3866 276
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI