Restriction map for
pFN21AB6659
(Circular) (Six-base) MAPSORT of: pFN21AB6659.seq Check: 7565 from: 1 to: 5977
pFN21AB6659 5977 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 278
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3320 1937
Size: 1383 4594
Acc65I G'GTAC_C
Cuts at: 3298 3298
Size: 5977
AclI AA'CG_TT
Cuts at: 1563 4104 4477 1563
Size: 2541 373 3063
Fragments arranged by size:
3063 2541 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2452 2495 2853 3270 4040 5424 5692 1632
Size: 820 43 358 417 770 1384 268 1917
Fragments arranged by size:
1917 1384 820 770 417 358 268 43
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5780
AflIII A'CryG_T
Cuts at: 1203 4876 5853 1203
Size: 3673 977 1327
Fragments arranged by size:
3673 1327 977
AgeI A'CCGG_T
Cuts at: 4808 4808
Size: 5977
AhdI GACnn_n'nnGTC
Cuts at: 4706 4706
Size: 5977
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5977
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5977
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2838 2870 2838
Size: 32 5945
AlwNI CAG_nnn'CTG
Cuts at: 2995 3720 5292 2995
Size: 725 1572 3680
Fragments arranged by size:
3680 1572 725
ApaLI G'TGCA_C
Cuts at: 4034 5190 4034
Size: 1156 4821
ApoI r'AATT_y
Cuts at: 1493 3286 3512 3664 5773 1493
Size: 1793 226 152 2109 1697
Fragments arranged by size:
2109 1793 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4531 160
Size: 872 3499 1606
Fragments arranged by size:
3499 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2335 3302 1870
Size: 73 147 245 967 4545
Fragments arranged by size:
4545 967 245 147 73
BamHI G'GATC_C
Cuts at: 3307 3674 3307
Size: 367 5610
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3298 4753 618
Size: 325 217 627 1511 1455 1842
Fragments arranged by size:
1842 1511 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 3005 3296 1391
Size: 654 960 291 4072
Fragments arranged by size:
4072 960 654 291
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3141 928
Size: 971 1242 3764
Fragments arranged by size:
3764 1242 971
BbvCI CC'TCA_GC
Cuts at: 2460 2460
Size: 5977
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2339 2373 2402 2436 4168 4202 1903
Size: 34 402 34 29 34 1732 34 3678
Fragments arranged by size:
3678 1732 402 34 34 34 34 29
BciVI GTATCCnnnnn_n'
Cuts at: 3869 5079 3869
Size: 1210 4767
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5977
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2339 4588 136
Size: 107 122 71 631 605 667 2249 1525
Fragments arranged by size:
2249 1525 667 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5972 5972
Size: 5977
BlpI GC'TnA_GC
Cuts at: 3394 3394
Size: 5977
Bme1580I G_kGCm'C
Cuts at: 1790 4038 5194 1790
Size: 2248 1156 2573
Fragments arranged by size:
2573 2248 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5977
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3708 4666 442
Size: 416 53 83 399 24 2291 958 1753
Fragments arranged by size:
2291 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2134 2142 2297 2679 2888 2972 3029 4637
Size: 895 8 155 382 209 84 57 1608
Cuts at: 4637 1239
Size: 2579
Fragments arranged by size:
2579 1608 895 382 209 155 84 57
8
Bpu10I CC'TnA_GC
Cuts at: 2039 2460 2039
Size: 421 5556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2912 4102 4967 5265 2912
Size: 1190 865 298 3624
Fragments arranged by size:
3624 1190 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2945 4640 882
Size: 617 1446 1695 2219
Fragments arranged by size:
2219 1695 1446 617
BsaAI yAC'GTr
Cuts at: 493 1204 3768 493
Size: 711 2564 2702
Fragments arranged by size:
2702 2564 711
BsaBI GATnn'nnATC
Cuts at: 5971 5971
Size: 5977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2712 3784 4166 4801
Size: 53 83 186 936 1179 1072 382 635
Cuts at: 4801 275
Size: 1451
Fragments arranged by size:
1451 1179 1072 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4410 4808 5082 5229 5640 4410
Size: 398 274 147 411 4747
Fragments arranged by size:
4747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3123 3153 1178
Size: 30 165 30 1720 30 4002
Fragments arranged by size:
4002 1720 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 2603 2963 1186
Size: 849 568 360 4200
Fragments arranged by size:
4200 849 568 360
BseYI C'CCAG_C
Cuts at: 2102 5180 5676 2102
Size: 3078 496 2403
Fragments arranged by size:
3078 2403 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5404
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3427 4188 5216 1540
Size: 275 186 1426 761 1028 2301
Fragments arranged by size:
2301 1426 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2045 2940 3005 4038 4123 5194 2045
Size: 895 65 1033 85 1071 2828
Fragments arranged by size:
2828 1071 1033 895 85 65
BsmI GAATG_Cn'
Cuts at: 3488 3581 3488
Size: 93 5884
BsmBI CGTCTCn'nnnn_
Cuts at: 2866 2866
Size: 5977
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2940 3005 3296 4038 4123 5194
Size: 399 255 895 65 291 742 85 1071
Cuts at: 5194 1391
Size: 2174
Fragments arranged by size:
2174 1071 895 742 399 291 255 85
65
BspEI T'CCGG_A
Cuts at: 5640 5640
Size: 5977
BspHI T'CATG_A
Cuts at: 2718 3866 5596 2718
Size: 1148 1730 3099
Fragments arranged by size:
3099 1730 1148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3332 844
Size: 2488 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3864 1394
Size: 429 2041 3507
Fragments arranged by size:
3507 2041 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2215 4472 4646 65
Size: 1183 967 2257 174 1396
Fragments arranged by size:
2257 1396 1183 967 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4621 4808 1700
Size: 110 2811 187 2869
Fragments arranged by size:
2869 2811 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5977
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5977
BssSI C'ACGA_G
Cuts at: 4037 5049 4037
Size: 1012 4965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5977
BstBI TT'CG_AA
Cuts at: 4781 4781
Size: 5977
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5977
BstYI r'GATC_y
Cuts at: 1972 3307 3674 4059 4076 5528 5614 5626 5972
Size: 1335 367 385 17 1452 86 12 346
Cuts at: 5972 1972
Size: 1977
Fragments arranged by size:
1977 1452 1335 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4486
Fragments arranged by size:
4486 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5238
Fragments arranged by size:
5238 683 56
BtsI GCAGTG_nn'
Cuts at: 2321 3493 4287 4307 2321
Size: 1172 794 20 3991
Fragments arranged by size:
3991 1172 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5977
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5942
DraI TTT'AAA
Cuts at: 3281 3281
Size: 5977
DraIII CAC_nnn'GTG
Cuts at: 2161 2161
Size: 5977
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4984 809
Size: 507 3668 1802
Fragments arranged by size:
3668 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2220 3424 4313
Size: 54 1425 50 275 18 390 1204 889
Cuts at: 4313 5942 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1204 889 390 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3424 1537
Size: 275 1612 4090
Fragments arranged by size:
4090 1612 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2373 3168 3907 1353
Size: 1020 795 739 3423
Fragments arranged by size:
3423 1020 795 739
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4564 4938 5084 387
Size: 1064 138 2975 374 146 1280
Fragments arranged by size:
2975 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2043 3003 2043
Size: 960 5017
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2134 2142 2297 2452 2495 2679 2853
Size: 393 502 8 155 155 43 184 174
Cuts at: 2853 2888 2972 3029 3270 4040 4637 5424 5692
Size: 35 84 57 241 770 597 787 268
Cuts at: 5692 1239
Size: 1524
Fragments arranged by size:
1524 787 770 597 502 393 268 241
184 174 155 155 84 57 43 35
8
EcoNI CCTnn'n_nnAGG
Cuts at: 2261 2534 2618 2768 2261
Size: 273 84 150 5470
Fragments arranged by size:
5470 273 150 84
EcoO109I rG'GnC_Cy
Cuts at: 2056 2194 2056
Size: 138 5839
EcoRI G'AATT_C
Cuts at: 3286 3664 3286
Size: 378 5599
FspI TGC'GCA
Cuts at: 4483 4483
Size: 5977
HaeII r_GCGC'y
Cuts at: 1678 2089 5124 1678
Size: 411 3035 2531
Fragments arranged by size:
3035 2531 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2329 2361 4618 4650 4692
Size: 32 135 32 757 32 2257 32 42
Cuts at: 4692 4724 1373
Size: 32 2626
Fragments arranged by size:
2626 2257 757 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3321 3567 669
Size: 988 281 1383 246 3079
Fragments arranged by size:
3079 1383 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2023 748
Size: 1275 4702
HpaI GTT'AAC
Cuts at: 3567 3567
Size: 5977
KpnI G_GTAC'C
Cuts at: 3302 3302
Size: 5977
MfeI C'AATT_G
Cuts at: 3576 3576
Size: 5977
MluI A'CGCG_T
Cuts at: 5853 5853
Size: 5977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2169 2310 5091 5275 1305
Size: 864 141 2781 184 2007
Fragments arranged by size:
2781 2007 864 184 141
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2222 10
Size: 54 1425 343 390 3765
Fragments arranged by size:
3765 1425 390 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3935 4294 4453 518
Size: 648 2769 359 159 2042
Fragments arranged by size:
2769 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3393 4070 5218 5463 1606
Size: 1787 677 1148 245 2120
Fragments arranged by size:
2120 1787 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4486
Fragments arranged by size:
4486 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5977
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5977
NotI GC'GGCC_GC
Cuts at: 3424 3424
Size: 5977
NruI TCG'CGA
Cuts at: 2349 2349
Size: 5977
NspI r_CATG'y
Cuts at: 1132 1229 2083 3335 4880 1132
Size: 97 854 1252 1545 2229
Fragments arranged by size:
2229 1545 1252 854 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5977
PciI A'CATG_T
Cuts at: 4876 4876
Size: 5977
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5977
PmeI GTTT'AAAC
Cuts at: 3281 3281
Size: 5977
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 5559 5591 4046
Size: 32 1481 32 4432
Fragments arranged by size:
4432 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2194 2194
Size: 5977
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5977
PsiI TTA'TAA
Cuts at: 3547 3547
Size: 5977
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3044 3076 3044
Size: 32 5945
PstI C_TGCA'G
Cuts at: 830 2803 2811 3329 830
Size: 1973 8 518 3478
Fragments arranged by size:
3478 1973 518 8
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5977
SacI G_AGCT'C
Cuts at: 2045 3005 2045
Size: 960 5017
SalI G'TCGA_C
Cuts at: 1936 3319 1936
Size: 1383 4594
SapI GCTCTTCn'nnn_
Cuts at: 2373 2373
Size: 5977
SbfI CC_TGCA'GG
Cuts at: 3329 3329
Size: 5977
ScaI AGT'ACT
Cuts at: 4225 5937 4225
Size: 1712 4265
SfcI C'TryA_G
Cuts at: 826 1045 2061 2353 2799 2807 3147 3325 4460
Size: 219 1016 292 446 8 340 178 1135
Cuts at: 4460 5141 5332 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1016 681 446 340 292 219
191 178 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5977
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5977
SmaI CCC'GGG
Cuts at: 1872 3304 1872
Size: 1432 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2891 4081 4982 5244 820
Size: 197 926 948 1190 901 262 1553
Fragments arranged by size:
1553 1190 948 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3768 493
Size: 3275 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5977
SphI G_CATG'C
Cuts at: 1132 2083 3335 1132
Size: 951 1252 3774
Fragments arranged by size:
3774 1252 951
SspI AAT'ATT
Cuts at: 5 52 3901 5
Size: 47 3849 2081
Fragments arranged by size:
3849 2081 47
StuI AGG'CCT
Cuts at: 1517 2529 2700 1517
Size: 1012 171 4794
Fragments arranged by size:
4794 1012 171
StyI C'CwwG_G
Cuts at: 513 1065 2004 2524 2707 2955 3416 513
Size: 552 939 520 183 248 461 3074
Fragments arranged by size:
3074 939 552 520 461 248 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2116 4004 4021 4174 4359 4804 325
Size: 1255 536 1888 17 153 185 445 1498
Fragments arranged by size:
1888 1498 1255 536 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2069 4223 5935
Size: 274 80 33 51 1443 92 2154 1712
Cuts at: 5935 96
Size: 138
Fragments arranged by size:
2154 1712 1443 274 138 92 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5937 5969 5937
Size: 32 5945
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5977
XbaI T'CTAG_A
Cuts at: 2944 3313 2944
Size: 369 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5977
XmaI C'CCGG_G
Cuts at: 1870 3302 1870
Size: 1432 4545
XmnI GAAnn'nnTTC
Cuts at: 4106 4106
Size: 5977
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 276
Size: 53 83 186 936 2251 2468
Fragments arranged by size:
2468 2251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NruI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI
PmlI PspOMI PspXI PvuII RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI