Restriction map for
pFN21AB6654
(Circular) (Six-base) MAPSORT of: pFN21AB6654.seq Check: 8800 from: 1 to: 5872
pFN21AB6654 5872 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3682 278
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2088 3215 1937
Size: 151 1127 4594
Fragments arranged by size:
4594 1127 151
Acc65I G'GTAC_C
Cuts at: 3193 3193
Size: 5872
AclI AA'CG_TT
Cuts at: 1563 3999 4372 1563
Size: 2436 373 3063
Fragments arranged by size:
3063 2436 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 3935 5319 5587 1632
Size: 458 1845 1384 268 1917
Fragments arranged by size:
1917 1845 1384 458 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5675
AflIII A'CryG_T
Cuts at: 1203 2674 4771 5748 1203
Size: 1471 2097 977 1327
Fragments arranged by size:
2097 1471 1327 977
AgeI A'CCGG_T
Cuts at: 4703 4703
Size: 5872
AhdI GACnn_n'nnGTC
Cuts at: 4601 4601
Size: 5872
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5872
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2895 2927 2895
Size: 32 5840
AlwNI CAG_nnn'CTG
Cuts at: 2053 3615 5187 2053
Size: 1562 1572 2738
Fragments arranged by size:
2738 1572 1562
ApaLI G'TGCA_C
Cuts at: 3929 5085 3929
Size: 1156 4716
ApoI r'AATT_y
Cuts at: 1493 2576 3145 3181 3407 3559 5668 1493
Size: 1083 569 36 226 152 2109 1697
Fragments arranged by size:
2109 1697 1083 569 226 152 36
AseI AT'TA_AT
Cuts at: 160 1032 4426 160
Size: 872 3394 1606
Fragments arranged by size:
3394 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
AvaI C'yCGr_G
Cuts at: 1870 1943 2366 3197 1870
Size: 73 423 831 4545
Fragments arranged by size:
4545 831 423 73
BamHI G'GATC_C
Cuts at: 3202 3569 3202
Size: 367 5505
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3193 4648 618
Size: 325 217 627 1406 1455 1842
Fragments arranged by size:
1842 1455 1406 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2079 2169 3191 1391
Size: 688 90 1022 4072
Fragments arranged by size:
4072 1022 688 90
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2528 928
Size: 971 629 4272
Fragments arranged by size:
4272 971 629
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4063 4097 1903
Size: 34 50 34 2042 34 3678
Fragments arranged by size:
3678 2042 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3764 4974 3764
Size: 1210 4662
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5872
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4483 136
Size: 107 122 71 631 605 2811 1525
Fragments arranged by size:
2811 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5867 5867
Size: 5872
BlpI GC'TnA_GC
Cuts at: 2078 3289 2078
Size: 1211 4661
Bme1580I G_kGCm'C
Cuts at: 1790 3933 5089 1790
Size: 2143 1156 2573
Fragments arranged by size:
2573 2143 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5872
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2359 2638 3017
Size: 416 53 83 399 24 942 279 379
Cuts at: 3017 3603 4561 442
Size: 586 958 1753
Fragments arranged by size:
1753 958 942 586 416 399 379 279
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5872
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4532 1239
Size: 3293 2579
Bpu10I CC'TnA_GC
Cuts at: 2114 2114
Size: 5872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2726 3997 4862 5160 2726
Size: 1271 865 298 3438
Fragments arranged by size:
3438 1271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4535 882
Size: 617 3036 2219
Fragments arranged by size:
3036 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3663 493
Size: 711 2459 2702
Fragments arranged by size:
2702 2459 711
BsaBI GATnn'nnATC
Cuts at: 5866 5866
Size: 5872
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3679 4061 4696 275
Size: 53 83 186 936 2146 382 635 1451
Fragments arranged by size:
2146 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2714 4305 4703 4977 5124 5535 2714
Size: 1591 398 274 147 411 3051
Fragments arranged by size:
3051 1591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5647
Fragments arranged by size:
5647 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2164 2333 2384 2876 1186
Size: 978 169 51 492 4182
Fragments arranged by size:
4182 978 492 169 51
BseYI C'CCAG_C
Cuts at: 5075 5571 5075
Size: 496 5376
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5299
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3322 4083 5111 1540
Size: 275 186 1321 761 1028 2301
Fragments arranged by size:
2301 1321 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3933 4018 5089 3933
Size: 85 1071 4716
Fragments arranged by size:
4716 1071 85
BsmI GAATG_Cn'
Cuts at: 3383 3476 3383
Size: 93 5779
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2169 3191 3933 4018 5089 1391
Size: 399 289 90 1022 742 85 1071 2174
Fragments arranged by size:
2174 1071 1022 742 399 289 90 85
BspEI T'CCGG_A
Cuts at: 5535 5535
Size: 5872
BspHI T'CATG_A
Cuts at: 3761 5491 3761
Size: 1730 4142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3227 844
Size: 2383 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2026 2118 3759 1394
Size: 429 203 92 1641 3507
Fragments arranged by size:
3507 1641 429 203 92
BsrDI GCAATG_nn'
Cuts at: 65 1248 4367 4541 65
Size: 1183 3119 174 1396
Fragments arranged by size:
3119 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 4516 4703 1700
Size: 110 297 2409 187 2869
Fragments arranged by size:
2869 2409 297 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5872
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5872
BssSI C'ACGA_G
Cuts at: 3932 4944 3932
Size: 1012 4860
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2053 794
Size: 1259 4613
BstBI TT'CG_AA
Cuts at: 4676 4676
Size: 5872
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5872
BstYI r'GATC_y
Cuts at: 1972 2154 2560 3202 3569 3954 3971 5423 5509
Size: 182 406 642 367 385 17 1452 86
Cuts at: 5509 5521 5867 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 642 406 385 367 346 182
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2169 2505 513
Size: 552 1104 336 3880
Fragments arranged by size:
3880 1104 552 336
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2430 487
Size: 683 56 1204 3929
Fragments arranged by size:
3929 1204 683 56
BtsI GCAGTG_nn'
Cuts at: 2700 3388 4182 4202 2700
Size: 688 794 20 4370
Fragments arranged by size:
4370 794 688 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5872
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5837
DraI TTT'AAA
Cuts at: 3176 3176
Size: 5872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4879 809
Size: 507 3563 1802
Fragments arranged by size:
3563 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 3319 4208
Size: 54 1425 50 275 18 303 1186 889
Cuts at: 4208 5837 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1186 889 303 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3319 1537
Size: 275 1507 4090
Fragments arranged by size:
4090 1507 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2588 3089 3802 1353
Size: 1235 501 713 3423
Fragments arranged by size:
3423 1235 713 501
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4459 4833 4979 387
Size: 1064 138 2870 374 146 1280
Fragments arranged by size:
2870 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 3935 4532 5319 5587 1239
Size: 393 458 1845 597 787 268 1524
Fragments arranged by size:
1845 1524 787 597 458 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 2918 2333
Size: 585 5287
EcoO109I rG'GnC_Cy
Cuts at: 2121 2121
Size: 5872
EcoRI G'AATT_C
Cuts at: 3145 3181 3559 3145
Size: 36 378 5458
Fragments arranged by size:
5458 378 36
EcoRV GAT'ATC
Cuts at: 2272 2272
Size: 5872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3100 3100
Size: 5872
FspI TGC'GCA
Cuts at: 4378 4378
Size: 5872
HaeII r_GCGC'y
Cuts at: 1678 5019 1678
Size: 3341 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3118 3150 4513 4545 4587
Size: 32 135 32 1546 32 1363 32 42
Cuts at: 4587 4619 1373
Size: 32 2626
Fragments arranged by size:
2626 1546 1363 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2089 3216 3462 669
Size: 988 281 151 1127 246 3079
Fragments arranged by size:
3079 1127 988 281 246 151
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5872
HpaI GTT'AAC
Cuts at: 3462 3462
Size: 5872
KpnI G_GTAC'C
Cuts at: 3197 3197
Size: 5872
MfeI C'AATT_G
Cuts at: 3471 3471
Size: 5872
MluI A'CGCG_T
Cuts at: 5748 5748
Size: 5872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3161 4986 5170 1305
Size: 1856 1825 184 2007
Fragments arranged by size:
2007 1856 1825 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 10
Size: 54 1425 343 303 3747
Fragments arranged by size:
3747 1425 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2945 2960 3830 4189 4348 518
Size: 648 1779 15 870 359 159 2042
Fragments arranged by size:
2042 1779 870 648 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 2053 2068 2192 2218 2481 3288 3965 5113
Size: 447 15 124 26 263 807 677 1148
Cuts at: 5113 5358 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 807 677 447 263 245 124
26 15
NaeI GCC'GGC
Cuts at: 2109 2109
Size: 5872
NcoI C'CATG_G
Cuts at: 513 1065 2505 513
Size: 552 1440 3880
Fragments arranged by size:
3880 1440 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5872
NgoMIV G'CCGG_C
Cuts at: 2107 2107
Size: 5872
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5872
NotI GC'GGCC_GC
Cuts at: 3319 3319
Size: 5872
NspI r_CATG'y
Cuts at: 1132 1229 3230 4775 1132
Size: 97 2001 1545 2229
Fragments arranged by size:
2229 2001 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5872
PciI A'CATG_T
Cuts at: 4771 4771
Size: 5872
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2395 1108
Size: 1287 4585
PfoI T'CCnGG_A
Cuts at: 2160 2160
Size: 5872
PmeI GTTT'AAAC
Cuts at: 3176 3176
Size: 5872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3941 3973 5454 5486 3941
Size: 32 1481 32 4327
Fragments arranged by size:
4327 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2121 2121
Size: 5872
PshAI GACnn'nnGTC
Cuts at: 1613 2126 1613
Size: 513 5359
PsiI TTA'TAA
Cuts at: 3442 3442
Size: 5872
PstI C_TGCA'G
Cuts at: 830 2058 2695 3224 830
Size: 1228 637 529 3478
Fragments arranged by size:
3478 1228 637 529
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5872
PvuII CAG'CTG
Cuts at: 2053 2192 2053
Size: 139 5733
SalI G'TCGA_C
Cuts at: 1936 2087 3214 1936
Size: 151 1127 4594
Fragments arranged by size:
4594 1127 151
SanDI GG'GwC_CC
Cuts at: 2121 2121
Size: 5872
SbfI CC_TGCA'GG
Cuts at: 3224 3224
Size: 5872
ScaI AGT'ACT
Cuts at: 4120 5832 4120
Size: 1712 4160
SexAI A'CCwGG_T
Cuts at: 2841 2841
Size: 5872
SfcI C'TryA_G
Cuts at: 826 1045 2054 2691 3220 4355 5036 5227 826
Size: 219 1009 637 529 1135 681 191 1471
Fragments arranged by size:
1471 1135 1009 681 637 529 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5872
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5872
SmaI CCC'GGG
Cuts at: 1872 3199 1872
Size: 1327 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2741 3976 4877 5139 820
Size: 197 926 798 1235 901 262 1553
Fragments arranged by size:
1553 1235 926 901 798 262 197
SnaBI TAC'GTA
Cuts at: 493 3663 493
Size: 3170 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5872
SphI G_CATG'C
Cuts at: 1132 3230 1132
Size: 2098 3774
SspI AAT'ATT
Cuts at: 5 52 3061 3796 5
Size: 47 3009 735 2081
Fragments arranged by size:
3009 2081 735 47
StuI AGG'CCT
Cuts at: 1517 2924 1517
Size: 1407 4465
StyI C'CwwG_G
Cuts at: 513 1065 2334 2505 3156 3311 513
Size: 552 1269 171 651 155 3074
Fragments arranged by size:
3074 1269 651 552 171 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3899 3916 4069 4254 4699 325
Size: 1255 2319 17 153 185 445 1498
Fragments arranged by size:
2319 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4118 5830 96
Size: 274 80 33 51 1443 2141 1712 138
Fragments arranged by size:
2141 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2339 2371 5832 5864 2339
Size: 32 3461 32 2347
Fragments arranged by size:
3461 2347 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5872
XbaI T'CTAG_A
Cuts at: 3208 3208
Size: 5872
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5872
XmaI C'CCGG_G
Cuts at: 1870 3197 1870
Size: 1327 4545
XmnI GAAnn'nnTTC
Cuts at: 4001 4001
Size: 5872
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3680 276
Size: 53 83 186 936 2146 2468
Fragments arranged by size:
2468 2146 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SfiI SfoI SrfI SwaI XcmI