Restriction map for
pFN21AB6653
(Circular) (Six-base) MAPSORT of: pFN21AB6653.seq Check: 9197 from: 1 to: 5932
pFN21AB6653 5932 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2189 2189
Size: 5932
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3742 278
Size: 53 83 186 936 2206 2468
Fragments arranged by size:
2468 2206 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2433 3275 1937
Size: 496 842 4594
Fragments arranged by size:
4594 842 496
Acc65I G'GTAC_C
Cuts at: 3253 3253
Size: 5932
AclI AA'CG_TT
Cuts at: 1563 4059 4432 1563
Size: 2496 373 3063
Fragments arranged by size:
3063 2496 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 2538 2722 3212 3995 5379 5647 1632
Size: 539 367 184 490 783 1384 268 1917
Fragments arranged by size:
1917 1384 783 539 490 367 268 184
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5735
AflIII A'CryG_T
Cuts at: 1203 4831 5808 1203
Size: 3628 977 1327
Fragments arranged by size:
3628 1327 977
AgeI A'CCGG_T
Cuts at: 2589 4763 2589
Size: 2174 3758
AhdI GACnn_n'nnGTC
Cuts at: 4661 4661
Size: 5932
AleI CACnn'nnGTG
Cuts at: 1166 2921 1166
Size: 1755 4177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5932
AlwNI CAG_nnn'CTG
Cuts at: 3675 5247 3675
Size: 1572 4360
ApaLI G'TGCA_C
Cuts at: 3989 5145 3989
Size: 1156 4776
ApoI r'AATT_y
Cuts at: 1493 3128 3241 3467 3619 5728 1493
Size: 1635 113 226 152 2109 1697
Fragments arranged by size:
2109 1697 1635 226 152 113
AseI AT'TA_AT
Cuts at: 160 1032 4486 160
Size: 872 3454 1606
Fragments arranged by size:
3454 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
AvaI C'yCGr_G
Cuts at: 1870 1943 2018 2603 2997 3257 1870
Size: 73 75 585 394 260 4545
Fragments arranged by size:
4545 585 394 260 75 73
BamHI G'GATC_C
Cuts at: 3262 3629 3262
Size: 367 5565
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2275 2933 3253 4708 618
Size: 325 217 627 488 658 320 1455 1842
Fragments arranged by size:
1842 1455 658 627 488 325 320 217
BanII G_rGCy'C
Cuts at: 1391 2048 2646 3251 1391
Size: 657 598 605 4072
Fragments arranged by size:
4072 657 605 598
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 2211 928
Size: 971 124 188 4649
Fragments arranged by size:
4649 971 188 124
BbvCI CC'TCA_GC
Cuts at: 2321 2321
Size: 5932
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4123 4157 1903
Size: 34 2186 34 3678
Fragments arranged by size:
3678 2186 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2404 3824 5034 2404
Size: 1420 1210 3302
Fragments arranged by size:
3302 1420 1210
BclI T'GATC_A
Cuts at: 1548 3220 1548
Size: 1672 4260
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4543 136
Size: 107 122 71 631 605 2871 1525
Fragments arranged by size:
2871 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5927 5927
Size: 5932
BlpI GC'TnA_GC
Cuts at: 2840 3349 2840
Size: 509 5423
Bme1580I G_kGCm'C
Cuts at: 1790 2280 3993 5149 1790
Size: 490 1713 1156 2573
Fragments arranged by size:
2573 1713 1156 490
BmgBI CAC'GTC
Cuts at: 1318 2665 1318
Size: 1347 4585
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2743 3073 3663
Size: 416 53 83 399 24 1326 330 590
Cuts at: 3663 4621 442
Size: 958 1753
Fragments arranged by size:
1753 1326 958 590 416 399 330 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4592 1239
Size: 3353 2579
Bpu10I CC'TnA_GC
Cuts at: 2321 2321
Size: 5932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2870 4057 4922 5220 2870
Size: 1187 865 298 3582
Fragments arranged by size:
3582 1187 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2870 4595 882
Size: 617 1371 1725 2219
Fragments arranged by size:
2219 1725 1371 617
BsaAI yAC'GTr
Cuts at: 493 1204 3723 493
Size: 711 2519 2702
Fragments arranged by size:
2702 2519 711
BsaBI GATnn'nnATC
Cuts at: 5926 5926
Size: 5932
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3739 4121 4756 275
Size: 53 83 186 936 2206 382 635 1451
Fragments arranged by size:
2206 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2589 3091 4365 4763 5037 5184 5595 2589
Size: 502 1274 398 274 147 411 2926
Fragments arranged by size:
2926 1274 502 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5707
Fragments arranged by size:
5707 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5932
BseYI C'CCAG_C
Cuts at: 2218 2329 2547 2798 2950 5135 5631 2218
Size: 111 218 251 152 2185 496 2519
Fragments arranged by size:
2519 2185 496 251 218 152 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2540 2582 1163
Size: 573 804 42 4513
Fragments arranged by size:
4513 804 573 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3382 4143 5171 1540
Size: 275 186 1381 761 1028 2301
Fragments arranged by size:
2301 1381 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3993 4078 5149 3993
Size: 85 1071 4776
Fragments arranged by size:
4776 1071 85
BsmI GAATG_Cn'
Cuts at: 3443 3536 3443
Size: 93 5839
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2280 2646 3251 3993 4078 5149
Size: 399 258 232 366 605 742 85 1071
Cuts at: 5149 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 605 399 366 258 232
85
BspEI T'CCGG_A
Cuts at: 3091 5595 3091
Size: 2504 3428
BspHI T'CATG_A
Cuts at: 3821 5551 3821
Size: 1730 4202
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2189 3287 844
Size: 1345 1098 3489
Fragments arranged by size:
3489 1345 1098
BsrBI CCG'CTC
Cuts at: 1394 1823 3089 3819 1394
Size: 429 1266 730 3507
Fragments arranged by size:
3507 1266 730 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4427 4601 65
Size: 1183 3179 174 1396
Fragments arranged by size:
3179 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2313 2589 4576 4763 1700
Size: 110 503 276 1987 187 2869
Fragments arranged by size:
2869 1987 503 276 187 110
BsrGI T'GTAC_A
Cuts at: 96 2820 96
Size: 2724 3208
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5932
BssSI C'ACGA_G
Cuts at: 2891 3992 5004 2891
Size: 1101 1012 3819
Fragments arranged by size:
3819 1101 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5932
BstBI TT'CG_AA
Cuts at: 4736 4736
Size: 5932
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5932
BstYI r'GATC_y
Cuts at: 1972 3262 3629 4014 4031 5483 5569 5581 5927
Size: 1290 367 385 17 1452 86 12 346
Cuts at: 5927 1972
Size: 1977
Fragments arranged by size:
1977 1452 1290 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2635 513
Size: 552 939 631 3810
Fragments arranged by size:
3810 939 631 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2233 487
Size: 683 56 1007 4186
Fragments arranged by size:
4186 1007 683 56
BtsI GCAGTG_nn'
Cuts at: 2390 2834 3448 4242 4262 2390
Size: 444 614 794 20 4060
Fragments arranged by size:
4060 794 614 444 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5932
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5897
DraI TTT'AAA
Cuts at: 3236 3236
Size: 5932
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4939 809
Size: 507 3623 1802
Fragments arranged by size:
3623 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2197 3379 4268
Size: 54 1425 50 275 18 367 1182 889
Cuts at: 4268 5897 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1182 889 367 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3379 1537
Size: 275 1567 4090
Fragments arranged by size:
4090 1567 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2466 2741 3862 1353
Size: 1113 275 1121 3423
Fragments arranged by size:
3423 1121 1113 275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4519 4893 5039 387
Size: 1064 138 2930 374 146 1280
Fragments arranged by size:
2930 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2538 2722 3212 3995 4592 5379
Size: 393 539 367 184 490 783 597 787
Cuts at: 5379 5647 1239
Size: 268 1524
Fragments arranged by size:
1524 787 783 597 539 490 393 367
268 184
EcoNI CCTnn'n_nnAGG
Cuts at: 2618 2618
Size: 5932
EcoRI G'AATT_C
Cuts at: 3241 3619 3241
Size: 378 5554
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2101 2101
Size: 5932
FspI TGC'GCA
Cuts at: 4438 4438
Size: 5932
HaeII r_GCGC'y
Cuts at: 1678 5079 1678
Size: 3401 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2777 2809 4573 4605 4647
Size: 32 135 32 1205 32 1764 32 42
Cuts at: 4647 4679 1373
Size: 32 2626
Fragments arranged by size:
2626 1764 1205 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3276 3522 669
Size: 988 281 1338 246 3079
Fragments arranged by size:
3079 1338 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5932
HpaI GTT'AAC
Cuts at: 3522 3522
Size: 5932
KpnI G_GTAC'C
Cuts at: 3257 3257
Size: 5932
MfeI C'AATT_G
Cuts at: 3531 3531
Size: 5932
MluI A'CGCG_T
Cuts at: 5808 5808
Size: 5932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5046 5230 1305
Size: 3741 184 2007
Fragments arranged by size:
3741 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4110
Fragments arranged by size:
4110 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2921 3890 4249 4408 518
Size: 648 1755 969 359 159 2042
Fragments arranged by size:
2042 1755 969 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3348 4025 5173 5418 1606
Size: 1742 677 1148 245 2120
Fragments arranged by size:
2120 1742 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2635 513
Size: 552 939 631 3810
Fragments arranged by size:
3810 939 631 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5932
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5932
NotI GC'GGCC_GC
Cuts at: 3379 3379
Size: 5932
NspI r_CATG'y
Cuts at: 1132 1229 2342 3290 4835 1132
Size: 97 1113 948 1545 2229
Fragments arranged by size:
2229 1545 1113 948 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5932
PciI A'CATG_T
Cuts at: 4831 4831
Size: 5932
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5932
PmeI GTTT'AAAC
Cuts at: 3236 3236
Size: 5932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4001 4033 5514 5546 4001
Size: 32 1481 32 4387
Fragments arranged by size:
4387 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2027 3079 3101 1613
Size: 414 1052 22 4444
Fragments arranged by size:
4444 1052 414 22
PsiI TTA'TAA
Cuts at: 3502 3502
Size: 5932
PspXI vC'TCGA_Gb
Cuts at: 2603 2603
Size: 5932
PstI C_TGCA'G
Cuts at: 830 2310 2397 3284 830
Size: 1480 87 887 3478
Fragments arranged by size:
3478 1480 887 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5932
SalI G'TCGA_C
Cuts at: 1936 3274 1936
Size: 1338 4594
SbfI CC_TGCA'GG
Cuts at: 2310 3284 2310
Size: 974 4958
ScaI AGT'ACT
Cuts at: 4180 5892 4180
Size: 1712 4220
SexAI A'CCwGG_T
Cuts at: 2787 2787
Size: 5932
SfcI C'TryA_G
Cuts at: 826 1045 2115 2306 2393 2968 3280 4415 5096
Size: 219 1070 191 87 575 312 1135 681
Cuts at: 5096 5287 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1070 681 575 312 219 191
191 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5932
SmaI CCC'GGG
Cuts at: 1872 2020 3259 1872
Size: 148 1239 4545
Fragments arranged by size:
4545 1239 148
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2603 2885 2997 4036 4937 5199
Size: 197 926 660 282 112 1039 901 262
Cuts at: 5199 820
Size: 1553
Fragments arranged by size:
1553 1039 926 901 660 282 262 197
112
SnaBI TAC'GTA
Cuts at: 493 3723 493
Size: 3230 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5932
SphI G_CATG'C
Cuts at: 1132 2342 3290 1132
Size: 1210 948 3774
Fragments arranged by size:
3774 1210 948
SspI AAT'ATT
Cuts at: 5 52 3856 5
Size: 47 3804 2081
Fragments arranged by size:
3804 2081 47
StuI AGG'CCT
Cuts at: 1517 2264 2423 1517
Size: 747 159 5026
Fragments arranged by size:
5026 747 159
StyI C'CwwG_G
Cuts at: 513 1065 2004 2635 3371 513
Size: 552 939 631 736 3074
Fragments arranged by size:
3074 939 736 631 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3959 3976 4129 4314 4759 325
Size: 1255 2379 17 153 185 445 1498
Fragments arranged by size:
2379 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2067 2628 2751
Size: 274 80 33 51 1443 90 561 123
Cuts at: 2751 2820 4178 5890 96
Size: 69 1358 1712 138
Fragments arranged by size:
1712 1443 1358 561 274 138 123 90
80 69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2559 2591 2946 2978 5892 5924 2559
Size: 32 355 32 2914 32 2567
Fragments arranged by size:
2914 2567 355 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5932
XbaI T'CTAG_A
Cuts at: 3268 3268
Size: 5932
XhoI C'TCGA_G
Cuts at: 1943 2603 2997 1943
Size: 660 394 4878
Fragments arranged by size:
4878 660 394
XmaI C'CCGG_G
Cuts at: 1870 2018 3257 1870
Size: 148 1239 4545
Fragments arranged by size:
4545 1239 148
XmnI GAAnn'nnTTC
Cuts at: 4061 4061
Size: 5932
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3740 276
Size: 53 83 186 936 2206 2468
Fragments arranged by size:
2468 2206 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI