Restriction map for
pFN21AB6651
(Circular) (Six-base) MAPSORT of: pFN21AB6651.seq Check: 8817 from: 1 to: 5965
pFN21AB6651 5965 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3775 278
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3308 1937
Size: 1371 4594
Acc65I G'GTAC_C
Cuts at: 3286 3286
Size: 5965
AclI AA'CG_TT
Cuts at: 1563 4092 4465 1563
Size: 2529 373 3063
Fragments arranged by size:
3063 2529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2562 4028 5412 5680 1632
Size: 930 1466 1384 268 1917
Fragments arranged by size:
1917 1466 1384 930 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5768
AflIII A'CryG_T
Cuts at: 1203 4864 5841 1203
Size: 3661 977 1327
Fragments arranged by size:
3661 1327 977
AgeI A'CCGG_T
Cuts at: 4796 4796
Size: 5965
AhdI GACnn_n'nnGTC
Cuts at: 4694 4694
Size: 5965
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3073 1184
Size: 1889 4076
AlwNI CAG_nnn'CTG
Cuts at: 2340 2792 3708 5280 2340
Size: 452 916 1572 3025
Fragments arranged by size:
3025 1572 916 452
ApaLI G'TGCA_C
Cuts at: 4022 5178 4022
Size: 1156 4809
ApoI r'AATT_y
Cuts at: 1493 2395 3274 3500 3652 5761 1493
Size: 902 879 226 152 2109 1697
Fragments arranged by size:
2109 1697 902 879 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4519 160
Size: 872 3487 1606
Fragments arranged by size:
3487 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
AvaI C'yCGr_G
Cuts at: 1870 1943 3290 1870
Size: 73 1347 4545
Fragments arranged by size:
4545 1347 73
BamHI G'GATC_C
Cuts at: 3295 3662 3295
Size: 367 5598
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3286 4741 618
Size: 325 217 627 1499 1455 1842
Fragments arranged by size:
1842 1499 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2945 2967 3284 1391
Size: 1554 22 317 4072
Fragments arranged by size:
4072 1554 317 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2430 928
Size: 971 531 4463
Fragments arranged by size:
4463 971 531
BbvCI CC'TCA_GC
Cuts at: 2615 2615
Size: 5965
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4156 4190 1903
Size: 34 2219 34 3678
Fragments arranged by size:
3678 2219 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3857 5067 3857
Size: 1210 4755
BclI T'GATC_A
Cuts at: 1548 2007 2167 2686 2713 1548
Size: 459 160 519 27 4800
Fragments arranged by size:
4800 519 459 160 27
BfrBI ATG'CAT
Cuts at: 2882 2882
Size: 5965
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4576 136
Size: 107 122 71 631 605 2904 1525
Fragments arranged by size:
2904 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3154 5960 3154
Size: 2806 3159
BlpI GC'TnA_GC
Cuts at: 3382 3382
Size: 5965
Bme1580I G_kGCm'C
Cuts at: 1790 4026 5182 1790
Size: 2236 1156 2573
Fragments arranged by size:
2573 2236 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5965
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2582 3696 4654
Size: 416 53 83 399 24 1165 1114 958
Cuts at: 4654 442
Size: 1753
Fragments arranged by size:
1753 1165 1114 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2641 2989 3255 3269 4625 1239
Size: 1402 348 266 14 1356 2579
Fragments arranged by size:
2579 1402 1356 348 266 14
Bpu10I CC'TnA_GC
Cuts at: 2615 2615
Size: 5965
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2121 3040 4090 4955 5253 2121
Size: 919 1050 865 298 2833
Fragments arranged by size:
2833 1050 919 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2379 3236 4628 882
Size: 617 880 857 1392 2219
Fragments arranged by size:
2219 1392 880 857 617
BsaAI yAC'GTr
Cuts at: 493 1204 3756 493
Size: 711 2552 2702
Fragments arranged by size:
2702 2552 711
BsaBI GATnn'nnATC
Cuts at: 5959 5959
Size: 5965
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3772 4154 4789 275
Size: 53 83 186 936 2239 382 635 1451
Fragments arranged by size:
2239 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4398 4796 5070 5217 5628 4398
Size: 398 274 147 411 4735
Fragments arranged by size:
4735 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5740
Fragments arranged by size:
5740 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5965
BseYI C'CCAG_C
Cuts at: 2340 3185 5168 5664 2340
Size: 845 1983 496 2641
Fragments arranged by size:
2641 1983 845 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2452 2971 1163
Size: 573 716 519 4157
Fragments arranged by size:
4157 716 573 519
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3415 4176 5204 1540
Size: 275 186 1414 761 1028 2301
Fragments arranged by size:
2301 1414 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2945 4026 4111 5182 2945
Size: 1081 85 1071 3728
Fragments arranged by size:
3728 1081 1071 85
BsmI GAATG_Cn'
Cuts at: 2552 2882 3476 3569 2552
Size: 330 594 93 4948
Fragments arranged by size:
4948 594 330 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2768 3191 2768
Size: 423 5542
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2945 2967 3284 4026 4111 5182 1391
Size: 399 1155 22 317 742 85 1071 2174
Fragments arranged by size:
2174 1155 1071 742 399 317 85 22
BspEI T'CCGG_A
Cuts at: 5628 5628
Size: 5965
BspHI T'CATG_A
Cuts at: 3854 5584 3854
Size: 1730 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3320 844
Size: 2476 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3852 1394
Size: 429 2029 3507
Fragments arranged by size:
3507 2029 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2806 4460 4634 65
Size: 1183 1558 1654 174 1396
Fragments arranged by size:
1654 1558 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4609 4796 1700
Size: 110 2799 187 2869
Fragments arranged by size:
2869 2799 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5965
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5965
BssSI C'ACGA_G
Cuts at: 4025 5037 4025
Size: 1012 4953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5965
BstBI TT'CG_AA
Cuts at: 4769 4769
Size: 5965
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5965
BstYI r'GATC_y
Cuts at: 1972 3154 3295 3662 4047 4064 5516 5602 5614
Size: 1182 141 367 385 17 1452 86 12
Cuts at: 5614 5960 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1182 385 367 346 141 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2496 2496
Size: 5965
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5413
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5226
Fragments arranged by size:
5226 683 56
BtsI GCAGTG_nn'
Cuts at: 2464 2943 3481 4275 4295 2464
Size: 479 538 794 20 4134
Fragments arranged by size:
4134 794 538 479 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5930
DraI TTT'AAA
Cuts at: 3269 3269
Size: 5965
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4972 809
Size: 507 3656 1802
Fragments arranged by size:
3656 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2331 3412 4301
Size: 54 1425 50 275 18 501 1081 889
Cuts at: 4301 5930 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1081 889 501 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3412 1537
Size: 275 1600 4090
Fragments arranged by size:
4090 1600 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3895 1353
Size: 2542 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4552 4926 5072 387
Size: 1064 138 2963 374 146 1280
Fragments arranged by size:
2963 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2943 2943
Size: 5965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2562 2641 2989 3255 3269 4028 4625
Size: 393 930 79 348 266 14 759 597
Cuts at: 4625 5412 5680 1239
Size: 787 268 1524
Fragments arranged by size:
1524 930 787 759 597 393 348 268
266 79 14
EcoO109I rG'GnC_Cy
Cuts at: 2679 2679
Size: 5965
EcoRI G'AATT_C
Cuts at: 3274 3652 3274
Size: 378 5587
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2477 2477
Size: 5965
FspI TGC'GCA
Cuts at: 4471 4471
Size: 5965
HaeII r_GCGC'y
Cuts at: 1678 5112 1678
Size: 3434 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4606 4638 4680 4712 1373
Size: 32 135 32 3034 32 42 32 2626
Fragments arranged by size:
3034 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2650 3309 3555 669
Size: 988 281 712 659 246 3079
Fragments arranged by size:
3079 988 712 659 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5965
HpaI GTT'AAC
Cuts at: 2650 3555 2650
Size: 905 5060
KpnI G_GTAC'C
Cuts at: 3290 3290
Size: 5965
MfeI C'AATT_G
Cuts at: 2388 3564 2388
Size: 1176 4789
MluI A'CGCG_T
Cuts at: 5841 5841
Size: 5965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5079 5263 1305
Size: 3774 184 2007
Fragments arranged by size:
3774 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2333 10
Size: 54 1425 343 501 3642
Fragments arranged by size:
3642 1425 501 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3923 4282 4441 518
Size: 648 2757 359 159 2042
Fragments arranged by size:
2757 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2340 2692 3381 4058 5206 5451 1606
Size: 734 352 689 677 1148 245 2120
Fragments arranged by size:
2120 1148 734 689 677 352 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5413
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5965
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5965
NotI GC'GGCC_GC
Cuts at: 3412 3412
Size: 5965
NsiI A_TGCA'T
Cuts at: 2884 2884
Size: 5965
NspI r_CATG'y
Cuts at: 1132 1229 2024 2592 3323 4868 1132
Size: 97 795 568 731 1545 2229
Fragments arranged by size:
2229 1545 795 731 568 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5965
PciI A'CATG_T
Cuts at: 4864 4864
Size: 5965
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2340 1108
Size: 1232 4733
PfoI T'CCnGG_A
Cuts at: 2377 2377
Size: 5965
PmeI GTTT'AAAC
Cuts at: 3269 3269
Size: 5965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2306 2338 4034 4066 5547 5579 2306
Size: 32 1696 32 1481 32 2692
Fragments arranged by size:
2692 1696 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5965
PsiI TTA'TAA
Cuts at: 3535 3535
Size: 5965
PstI C_TGCA'G
Cuts at: 830 2313 3317 830
Size: 1483 1004 3478
Fragments arranged by size:
3478 1483 1004
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5965
PvuII CAG'CTG
Cuts at: 2340 2692 2340
Size: 352 5613
SacI G_AGCT'C
Cuts at: 2945 2945
Size: 5965
SalI G'TCGA_C
Cuts at: 1936 3307 1936
Size: 1371 4594
SbfI CC_TGCA'GG
Cuts at: 3317 3317
Size: 5965
ScaI AGT'ACT
Cuts at: 4213 5925 4213
Size: 1712 4253
SfcI C'TryA_G
Cuts at: 826 1045 2309 2418 2521 3313 4448 5129 5320
Size: 219 1264 109 103 792 1135 681 191
Cuts at: 5320 826
Size: 1471
Fragments arranged by size:
1471 1264 1135 792 681 219 191 109
103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5965
SmaI CCC'GGG
Cuts at: 1872 3292 1872
Size: 1420 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2136 3019 4069 4970 5232 820
Size: 197 926 193 883 1050 901 262 1553
Fragments arranged by size:
1553 1050 926 901 883 262 197 193
SnaBI TAC'GTA
Cuts at: 493 3756 493
Size: 3263 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5965
SphI G_CATG'C
Cuts at: 1132 2592 3323 1132
Size: 1460 731 3774
Fragments arranged by size:
3774 1460 731
SspI AAT'ATT
Cuts at: 5 52 3889 5
Size: 47 3837 2081
Fragments arranged by size:
3837 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5965
StyI C'CwwG_G
Cuts at: 513 1065 3404 513
Size: 552 2339 3074
Fragments arranged by size:
3074 2339 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3992 4009 4162 4347 4792 325
Size: 1255 2412 17 153 185 445 1498
Fragments arranged by size:
2412 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4211 5923 96
Size: 274 80 33 51 1443 2234 1712 138
Fragments arranged by size:
2234 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5925 5957 5925
Size: 32 5933
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5965
XbaI T'CTAG_A
Cuts at: 2191 3301 2191
Size: 1110 4855
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5965
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5965
XmaI C'CCGG_G
Cuts at: 1870 3290 1870
Size: 1420 4545
XmnI GAAnn'nnTTC
Cuts at: 4094 4094
Size: 5965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3773 276
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI