Restriction map for
pFN21AB6650
(Circular) (Six-base) MAPSORT of: pFN21AB6650.seq Check: 3963 from: 1 to: 6043
pFN21AB6650 6043 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2118 3853 278
Size: 53 83 186 936 582 1735 2468
Fragments arranged by size:
2468 1735 936 582 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3386 1937
Size: 1449 4594
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2501 2687 3075 4106 5490 5758 1632
Size: 869 186 388 1031 1384 268 1917
Fragments arranged by size:
1917 1384 1031 869 388 268 186
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 2120 3324 4942 5919 1203
Size: 917 1204 1618 977 1327
Fragments arranged by size:
1618 1327 1204 977 917
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 4772 4772
Size: 6043
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6043
AlwNI CAG_nnn'CTG
Cuts at: 2479 3786 5358 2479
Size: 1307 1572 3164
Fragments arranged by size:
3164 1572 1307
ApaLI G'TGCA_C
Cuts at: 4100 5256 4100
Size: 1156 4887
ApoI r'AATT_y
Cuts at: 1493 3352 3578 3730 5839 1493
Size: 1859 226 152 2109 1697
Fragments arranged by size:
2109 1859 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2912 4597 160
Size: 872 1880 1685 1606
Fragments arranged by size:
1880 1685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 2849 3368 1870
Size: 73 906 519 4545
Fragments arranged by size:
4545 906 519 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2476 2509 2476
Size: 33 6010
BamHI G'GATC_C
Cuts at: 3373 3740 3373
Size: 367 5676
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3364 4819 618
Size: 325 217 627 1577 1455 1842
Fragments arranged by size:
1842 1577 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2535 3362 1391
Size: 1144 827 4072
Fragments arranged by size:
4072 1144 827
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2201 2403 3324 928
Size: 971 302 202 921 3647
Fragments arranged by size:
3647 971 921 302 202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4234 4268 1903
Size: 34 2297 34 3678
Fragments arranged by size:
3678 2297 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3161 3935 5145 3161
Size: 774 1210 4059
Fragments arranged by size:
4059 1210 774
BclI T'GATC_A
Cuts at: 1548 3223 1548
Size: 1675 4368
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4654 136
Size: 107 122 71 631 605 2982 1525
Fragments arranged by size:
2982 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3285 3309 6038 3285
Size: 24 2729 3290
Fragments arranged by size:
3290 2729 24
BlpI GC'TnA_GC
Cuts at: 2529 3460 2529
Size: 931 5112
Bme1580I G_kGCm'C
Cuts at: 1790 4104 5260 1790
Size: 2314 1156 2573
Fragments arranged by size:
2573 2314 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6043
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3774 4732 442
Size: 416 53 83 399 24 2357 958 1753
Fragments arranged by size:
2357 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2127 2486 4703 1239
Size: 888 359 2217 2579
Fragments arranged by size:
2579 2217 888 359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2706 4168 5033 5331 2706
Size: 1462 865 298 3418
Fragments arranged by size:
3418 1462 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2006 4706 882
Size: 617 507 2700 2219
Fragments arranged by size:
2700 2219 617 507
BsaAI yAC'GTr
Cuts at: 493 1204 3325 3834 493
Size: 711 2121 509 2702
Fragments arranged by size:
2702 2121 711 509
BsaBI GATnn'nnATC
Cuts at: 2174 2684 6037 2174
Size: 510 3353 2180
Fragments arranged by size:
3353 2180 510
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 3850 4232 4867
Size: 53 83 186 936 582 1735 382 635
Cuts at: 4867 275
Size: 1451
Fragments arranged by size:
1735 1451 936 635 582 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4476 4874 5148 5295 5706 4476
Size: 398 274 147 411 4813
Fragments arranged by size:
4813 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2100 2130 1178
Size: 30 165 30 697 30 5091
Fragments arranged by size:
5091 697 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2564 1186
Size: 838 540 4665
Fragments arranged by size:
4665 838 540
BseYI C'CCAG_C
Cuts at: 2139 2709 3170 3292 5246 5742 2139
Size: 570 461 122 1954 496 2440
Fragments arranged by size:
2440 1954 570 496 461 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5470
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3493 4254 5282 1540
Size: 275 186 1492 761 1028 2301
Fragments arranged by size:
2301 1492 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2535 4104 4189 5260 2535
Size: 1569 85 1071 3318
Fragments arranged by size:
3318 1569 1071 85
BsmI GAATG_Cn'
Cuts at: 3554 3647 3554
Size: 93 5950
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2535 3362 4104 4189 5260 1391
Size: 399 745 827 742 85 1071 2174
Fragments arranged by size:
2174 1071 827 745 742 399 85
BspEI T'CCGG_A
Cuts at: 5706 5706
Size: 6043
BspHI T'CATG_A
Cuts at: 3932 5662 3932
Size: 1730 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2304 3398 844
Size: 1460 1094 3489
Fragments arranged by size:
3489 1460 1094
BsrBI CCG'CTC
Cuts at: 1394 1823 3178 3930 1394
Size: 429 1355 752 3507
Fragments arranged by size:
3507 1355 752 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2898 4538 4712 65
Size: 1183 1650 1640 174 1396
Fragments arranged by size:
1650 1640 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4687 4874 1700
Size: 110 2877 187 2869
Fragments arranged by size:
2877 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6043
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6043
BssSI C'ACGA_G
Cuts at: 4103 5115 4103
Size: 1012 5031
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6043
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6043
BstYI r'GATC_y
Cuts at: 1972 2023 3285 3309 3373 3740 4125 4142 5594
Size: 51 1262 24 64 367 385 17 1452
Cuts at: 5594 5680 5692 6038 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1262 385 367 346 86 64
51 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4552
Fragments arranged by size:
4552 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5304
Fragments arranged by size:
5304 683 56
BtsI GCAGTG_nn'
Cuts at: 3559 4353 4373 3559
Size: 794 20 5229
Fragments arranged by size:
5229 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5050 809
Size: 507 3734 1802
Fragments arranged by size:
3734 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3490 4379 6008
Size: 54 1425 50 275 18 1660 889 1629
Cuts at: 6008 8
Size: 43
Fragments arranged by size:
1660 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3490 1537
Size: 275 1678 4090
Fragments arranged by size:
4090 1678 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2427 3014 3973 1353
Size: 1074 587 959 3423
Fragments arranged by size:
3423 1074 959 587
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4630 5004 5150 387
Size: 1064 138 3041 374 146 1280
Fragments arranged by size:
3041 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2533 2533
Size: 6043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2486 2501 2687 3075 4106 4703
Size: 393 495 359 15 186 388 1031 597
Cuts at: 4703 5490 5758 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1031 787 597 495 393 388 359
268 186 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2300 2300
Size: 6043
EcoRI G'AATT_C
Cuts at: 3352 3730 3352
Size: 378 5665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2385 3328 2385
Size: 943 5100
FspI TGC'GCA
Cuts at: 4549 4549
Size: 6043
HaeII r_GCGC'y
Cuts at: 1678 5190 1678
Size: 3512 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2100 2132 4684 4716 4758
Size: 32 135 32 528 32 2552 32 42
Cuts at: 4758 4790 1373
Size: 32 2626
Fragments arranged by size:
2626 2552 528 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2718 3387 3633 669
Size: 988 281 780 669 246 3079
Fragments arranged by size:
3079 988 780 669 281 246
HindIII A'AGCT_T
Cuts at: 748 2286 2741 748
Size: 1538 455 4050
Fragments arranged by size:
4050 1538 455
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3265 3642 3265
Size: 377 5666
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 5157 5341 1305
Size: 1260 2592 184 2007
Fragments arranged by size:
2592 2007 1260 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4221
Fragments arranged by size:
4221 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2219 4001 4360 4519 518
Size: 648 1053 1782 359 159 2042
Fragments arranged by size:
2042 1782 1053 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 2490 2713 2785 3459 4136 5284 5529
Size: 433 451 223 72 674 677 1148 245
Cuts at: 5529 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 674 451 433 245 223
72
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4552
Fragments arranged by size:
4552 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6043
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 2124 2524 3401 4946 1132
Size: 97 895 400 877 1545 2229
Fragments arranged by size:
2229 1545 895 877 400 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6043
PciI A'CATG_T
Cuts at: 2120 4942 2120
Size: 2822 3221
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3146 1108
Size: 2038 4005
PfoI T'CCnGG_A
Cuts at: 3118 3118
Size: 6043
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PmlI CAC'GTG
Cuts at: 3325 3325
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PspXI vC'TCGA_Gb
Cuts at: 2849 2849
Size: 6043
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2306 2338 2306
Size: 32 6011
PstI C_TGCA'G
Cuts at: 830 2979 3395 830
Size: 2149 416 3478
Fragments arranged by size:
3478 2149 416
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
PvuII CAG'CTG
Cuts at: 2039 2490 2713 2785 2039
Size: 451 223 72 5297
Fragments arranged by size:
5297 451 223 72
SacI G_AGCT'C
Cuts at: 2535 2535
Size: 6043
SalI G'TCGA_C
Cuts at: 1936 3385 1936
Size: 1449 4594
SbfI CC_TGCA'GG
Cuts at: 3395 3395
Size: 6043
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SfcI C'TryA_G
Cuts at: 826 1045 2564 2975 3297 3391 4526 5207 5398
Size: 219 1519 411 322 94 1135 681 191
Cuts at: 5398 826
Size: 1471
Fragments arranged by size:
1519 1471 1135 681 411 322 219 191
94
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6043
SmaI CCC'GGG
Cuts at: 1872 3370 1872
Size: 1498 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2721 2849 4147 5048 5310 820
Size: 197 926 778 128 1298 901 262 1553
Fragments arranged by size:
1553 1298 926 901 778 262 197 128
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3401 1132
Size: 2269 3774
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6043
StyI C'CwwG_G
Cuts at: 513 1065 2004 2301 3231 3482 513
Size: 552 939 297 930 251 3074
Fragments arranged by size:
3074 939 930 552 297 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2153 4070 4087 4240 4425 4870 325
Size: 1255 573 1917 17 153 185 445 1498
Fragments arranged by size:
1917 1498 1255 573 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4289 6001 96
Size: 274 80 33 51 1443 2312 1712 138
Fragments arranged by size:
2312 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2676 2708 6003 6035 2676
Size: 32 3295 32 2684
Fragments arranged by size:
3295 2684 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6043
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XhoI C'TCGA_G
Cuts at: 1943 2849 1943
Size: 906 5137
XmaI C'CCGG_G
Cuts at: 1870 3368 1870
Size: 1498 4545
XmnI GAAnn'nnTTC
Cuts at: 4172 4172
Size: 6043
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2116 3851 276
Size: 53 83 186 936 582 1735 2468
Fragments arranged by size:
2468 1735 936 582 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI