Restriction map for
pFN21AB6643
(Circular) (Six-base) MAPSORT of: pFN21AB6643.seq Check: 1387 from: 1 to: 5866
pFN21AB6643 5866 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3676 278
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2181 3209 1937
Size: 244 1028 4594
Fragments arranged by size:
4594 1028 244
Acc65I G'GTAC_C
Cuts at: 3187 3187
Size: 5866
AclI AA'CG_TT
Cuts at: 1563 3993 4366 1563
Size: 2430 373 3063
Fragments arranged by size:
3063 2430 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2304 3929 5313 5581 1632
Size: 672 1625 1384 268 1917
Fragments arranged by size:
1917 1625 1384 672 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5669
AflIII A'CryG_T
Cuts at: 1203 4765 5742 1203
Size: 3562 977 1327
Fragments arranged by size:
3562 1327 977
AgeI A'CCGG_T
Cuts at: 4697 4697
Size: 5866
AhdI GACnn_n'nnGTC
Cuts at: 2547 4595 2547
Size: 2048 3818
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5866
AlwNI CAG_nnn'CTG
Cuts at: 3609 5181 3609
Size: 1572 4294
ApaI G_GGCC'C
Cuts at: 2783 2783
Size: 5866
ApaLI G'TGCA_C
Cuts at: 3923 5079 3923
Size: 1156 4710
ApoI r'AATT_y
Cuts at: 1493 2887 3175 3401 3553 5662 1493
Size: 1394 288 226 152 2109 1697
Fragments arranged by size:
2109 1697 1394 288 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4420 160
Size: 872 3388 1606
Fragments arranged by size:
3388 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 3191 1870
Size: 73 83 1165 4545
Fragments arranged by size:
4545 1165 83 73
BamHI G'GATC_C
Cuts at: 2477 3196 3563 2477
Size: 719 367 4780
Fragments arranged by size:
4780 719 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3187 4642 618
Size: 325 217 627 1400 1455 1842
Fragments arranged by size:
1842 1455 1400 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2783 3185 1391
Size: 1392 402 4072
Fragments arranged by size:
4072 1392 402
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2814 928
Size: 971 915 3980
Fragments arranged by size:
3980 971 915
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4057 4091 1903
Size: 34 2120 34 3678
Fragments arranged by size:
3678 2120 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3048 3758 4968 3048
Size: 710 1210 3946
Fragments arranged by size:
3946 1210 710
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5866
BfrBI ATG'CAT
Cuts at: 2623 2915 2623
Size: 292 5574
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4477 136
Size: 107 122 71 631 605 2805 1525
Fragments arranged by size:
2805 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5861 5861
Size: 5866
BlpI GC'TnA_GC
Cuts at: 3283 3283
Size: 5866
Bme1580I G_kGCm'C
Cuts at: 1790 2783 3927 5083 1790
Size: 993 1144 1156 2573
Fragments arranged by size:
2573 1156 1144 993
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5866
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3597 4555 442
Size: 416 53 83 399 24 2180 958 1753
Fragments arranged by size:
2180 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5866
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2304 2304
Size: 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2286 2835 4526 1239
Size: 1047 549 1691 2579
Fragments arranged by size:
2579 1691 1047 549
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3991 4856 5154 3991
Size: 865 298 4703
Fragments arranged by size:
4703 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4529 882
Size: 617 3030 2219
Fragments arranged by size:
3030 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3657 493
Size: 711 2453 2702
Fragments arranged by size:
2702 2453 711
BsaBI GATnn'nnATC
Cuts at: 2205 5860 2205
Size: 3655 2211
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3673 4055 4690 275
Size: 53 83 186 936 2140 382 635 1451
Fragments arranged by size:
2140 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4299 4697 4971 5118 5529 4299
Size: 398 274 147 411 4636
Fragments arranged by size:
4636 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5641
Fragments arranged by size:
5641 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2760 1186
Size: 1574 4292
BseYI C'CCAG_C
Cuts at: 2952 3114 5069 5565 2952
Size: 162 1955 496 3253
Fragments arranged by size:
3253 1955 496 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2334 1163
Size: 573 598 4695
Fragments arranged by size:
4695 598 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3316 4077 5105 1540
Size: 275 186 1315 761 1028 2301
Fragments arranged by size:
2301 1315 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3927 4012 5083 3927
Size: 85 1071 4710
Fragments arranged by size:
4710 1071 85
BsmI GAATG_Cn'
Cuts at: 3377 3470 3377
Size: 93 5773
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2783 3185 3927 4012 5083 1391
Size: 399 993 402 742 85 1071 2174
Fragments arranged by size:
2174 1071 993 742 402 399 85
BspEI T'CCGG_A
Cuts at: 5529 5529
Size: 5866
BspHI T'CATG_A
Cuts at: 3755 5485 3755
Size: 1730 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3154 3221 844
Size: 2310 67 3489
Fragments arranged by size:
3489 2310 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2283 3753 1394
Size: 429 460 1470 3507
Fragments arranged by size:
3507 1470 460 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4361 4535 65
Size: 1183 3113 174 1396
Fragments arranged by size:
3113 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4510 4697 1700
Size: 110 2700 187 2869
Fragments arranged by size:
2869 2700 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5866
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5866
BssSI C'ACGA_G
Cuts at: 3926 4938 3926
Size: 1012 4854
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2164 794
Size: 1370 4496
BstBI TT'CG_AA
Cuts at: 4670 4670
Size: 5866
BstEII G'GTnAC_C
Cuts at: 1184 2981 1184
Size: 1797 4069
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2387 2011
Size: 376 5490
BstYI r'GATC_y
Cuts at: 1972 2477 3196 3563 3948 3965 5417 5503 5515
Size: 505 719 367 385 17 1452 86 12
Cuts at: 5515 5861 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 719 505 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2182 2182
Size: 5866
BtgI C'CryG_G
Cuts at: 513 1065 2473 2481 513
Size: 552 1408 8 3898
Fragments arranged by size:
3898 1408 552 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5127
Fragments arranged by size:
5127 683 56
BtsI GCAGTG_nn'
Cuts at: 3382 4176 4196 3382
Size: 794 20 5052
Fragments arranged by size:
5052 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5831
DraI TTT'AAA
Cuts at: 3170 3170
Size: 5866
DraIII CAC_nnn'GTG
Cuts at: 3020 3020
Size: 5866
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4873 809
Size: 507 3557 1802
Fragments arranged by size:
3557 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2377 2899 3313
Size: 54 1425 50 275 18 547 522 414
Cuts at: 3313 4202 5831 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 547 522 414 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3313 1537
Size: 275 1501 4090
Fragments arranged by size:
4090 1501 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3796 1353
Size: 2443 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2682 4453 4827 4973 387
Size: 1064 138 1093 1771 374 146 1280
Fragments arranged by size:
1771 1280 1093 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2304 2835 3929 4526 5313 5581
Size: 393 654 18 531 1094 597 787 268
Cuts at: 5581 1239
Size: 1524
Fragments arranged by size:
1524 1094 787 654 597 531 393 268
18
EcoNI CCTnn'n_nnAGG
Cuts at: 2853 2853
Size: 5866
EcoO109I rG'GnC_Cy
Cuts at: 2261 2342 2414 2779 2846 3029 2261
Size: 81 72 365 67 183 5098
Fragments arranged by size:
5098 365 183 81 72 67
EcoRI G'AATT_C
Cuts at: 3175 3553 3175
Size: 378 5488
EcoRV GAT'ATC
Cuts at: 2945 2945
Size: 5866
FspI TGC'GCA
Cuts at: 4372 4372
Size: 5866
HaeII r_GCGC'y
Cuts at: 1678 5013 1678
Size: 3335 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2533 2565 4507 4539 4581
Size: 32 135 32 961 32 1942 32 42
Cuts at: 4581 4613 1373
Size: 32 2626
Fragments arranged by size:
2626 1942 961 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2719 3210 3456 669
Size: 988 281 781 491 246 3079
Fragments arranged by size:
3079 988 781 491 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5866
HpaI GTT'AAC
Cuts at: 2719 3456 2719
Size: 737 5129
KpnI G_GTAC'C
Cuts at: 3191 3191
Size: 5866
MfeI C'AATT_G
Cuts at: 3465 3465
Size: 5866
MluI A'CGCG_T
Cuts at: 5742 5742
Size: 5866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 4980 5164 1305
Size: 753 2922 184 2007
Fragments arranged by size:
2922 2007 753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2901 10
Size: 54 1425 343 1069 2975
Fragments arranged by size:
2975 1425 1069 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2456 3824 4183 4342 518
Size: 648 843 447 1368 359 159 2042
Fragments arranged by size:
2042 1368 843 648 447 359 159
MspA1I CmG'CkG
Cuts at: 1606 2060 3282 3959 5107 5352 1606
Size: 454 1222 677 1148 245 2120
Fragments arranged by size:
2120 1222 1148 677 454 245
NcoI C'CATG_G
Cuts at: 513 1065 2473 2481 513
Size: 552 1408 8 3898
Fragments arranged by size:
3898 1408 552 8
NdeI CA'TA_TG
Cuts at: 387 2400 2733 387
Size: 2013 333 3520
Fragments arranged by size:
3520 2013 333
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5866
NotI GC'GGCC_GC
Cuts at: 3313 3313
Size: 5866
NsiI A_TGCA'T
Cuts at: 2625 2917 2625
Size: 292 5574
NspI r_CATG'y
Cuts at: 1132 1229 2317 2362 2524 3224 4769 1132
Size: 97 1088 45 162 700 1545 2229
Fragments arranged by size:
2229 1545 1088 700 162 97 45
PasI CC'CwG_GG
Cuts at: 1348 2969 1348
Size: 1621 4245
PciI A'CATG_T
Cuts at: 4765 4765
Size: 5866
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5866
PfoI T'CCnGG_A
Cuts at: 2263 2263
Size: 5866
PmeI GTTT'AAAC
Cuts at: 3170 3170
Size: 5866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3935 3967 5448 5480 3935
Size: 32 1481 32 4321
Fragments arranged by size:
4321 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2261 2342 2414 2846 3029 2261
Size: 81 72 432 183 5098
Fragments arranged by size:
5098 432 183 81 72
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5866
PsiI TTA'TAA
Cuts at: 3436 3436
Size: 5866
PspOMI G'GGCC_C
Cuts at: 2779 2779
Size: 5866
PstI C_TGCA'G
Cuts at: 830 3218 830
Size: 2388 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5866
SalI G'TCGA_C
Cuts at: 1936 3208 1936
Size: 1272 4594
SbfI CC_TGCA'GG
Cuts at: 3218 3218
Size: 5866
ScaI AGT'ACT
Cuts at: 2795 4114 5826 2795
Size: 1319 1712 2835
Fragments arranged by size:
2835 1712 1319
SfcI C'TryA_G
Cuts at: 826 1045 2761 3214 4349 5030 5221 826
Size: 219 1716 453 1135 681 191 1471
Fragments arranged by size:
1716 1471 1135 681 453 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5866
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5866
SmaI CCC'GGG
Cuts at: 1872 3193 1872
Size: 1321 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3970 4871 5133 820
Size: 197 926 2027 901 262 1553
Fragments arranged by size:
2027 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3657 493
Size: 3164 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5866
SphI G_CATG'C
Cuts at: 1132 2524 3224 1132
Size: 1392 700 3774
Fragments arranged by size:
3774 1392 700
SspI AAT'ATT
Cuts at: 5 52 2873 3790 5
Size: 47 2821 917 2081
Fragments arranged by size:
2821 2081 917 47
StuI AGG'CCT
Cuts at: 1517 3124 1517
Size: 1607 4259
StyI C'CwwG_G
Cuts at: 513 1065 2473 2481 2608 2680 2722 3305 513
Size: 552 1408 8 127 72 42 583 3074
Fragments arranged by size:
3074 1408 583 552 127 72 42 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2254 3893 3910 4063 4248 4693 325
Size: 1255 674 1639 17 153 185 445 1498
Fragments arranged by size:
1639 1498 1255 674 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2527 2793 4112
Size: 274 80 33 51 1443 550 266 1319
Cuts at: 4112 5824 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1319 550 274 266 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5826 5858 5826
Size: 32 5834
Tth111I GACn'n_nGTC
Cuts at: 1316 2844 3060 1316
Size: 1528 216 4122
Fragments arranged by size:
4122 1528 216
XbaI T'CTAG_A
Cuts at: 3202 3202
Size: 5866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2831 3146 2831
Size: 315 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5866
XmaI C'CCGG_G
Cuts at: 1870 3191 1870
Size: 1321 4545
XmnI GAAnn'nnTTC
Cuts at: 3995 3995
Size: 5866
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3674 276
Size: 53 83 186 936 2140 2468
Fragments arranged by size:
2468 2140 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI Bsu36I EcoICRI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI