Restriction map for
pFN21AB6642
(Circular) (Six-base) MAPSORT of: pFN21AB6642.seq Check: 1853 from: 1 to: 6034
pFN21AB6642 6034 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2462 2462
Size: 6034
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3844 278
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3108 3377 1937
Size: 1171 269 4594
Fragments arranged by size:
4594 1171 269
Acc65I G'GTAC_C
Cuts at: 3355 3355
Size: 6034
AclI AA'CG_TT
Cuts at: 1563 4161 4534 1563
Size: 2598 373 3063
Fragments arranged by size:
3063 2598 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 4097 5481 5749 1632
Size: 483 1982 1384 268 1917
Fragments arranged by size:
1982 1917 1384 483 268
AfeI AGC'GCT
Cuts at: 2651 2651
Size: 6034
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5837
AflIII A'CryG_T
Cuts at: 1203 3081 4933 5910 1203
Size: 1878 1852 977 1327
Fragments arranged by size:
1878 1852 1327 977
AgeI A'CCGG_T
Cuts at: 4865 4865
Size: 6034
AhdI GACnn_n'nnGTC
Cuts at: 4763 4763
Size: 6034
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6034
AlwNI CAG_nnn'CTG
Cuts at: 2395 2830 3777 5349 2395
Size: 435 947 1572 3080
Fragments arranged by size:
3080 1572 947 435
ApaI G_GGCC'C
Cuts at: 2484 2638 3227 2484
Size: 154 589 5291
Fragments arranged by size:
5291 589 154
ApaLI G'TGCA_C
Cuts at: 2449 3035 4091 5247 2449
Size: 586 1056 1156 3236
Fragments arranged by size:
3236 1156 1056 586
ApoI r'AATT_y
Cuts at: 1493 3265 3343 3569 3721 5830 1493
Size: 1772 78 226 152 2109 1697
Fragments arranged by size:
2109 1772 1697 226 152 78
AseI AT'TA_AT
Cuts at: 160 1032 4588 160
Size: 872 3556 1606
Fragments arranged by size:
3556 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
AvaI C'yCGr_G
Cuts at: 1870 1943 2030 2483 3228 3359 1870
Size: 73 87 453 745 131 4545
Fragments arranged by size:
4545 745 453 131 87 73
BamHI G'GATC_C
Cuts at: 3364 3731 3364
Size: 367 5667
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3116 3355 4810 618
Size: 325 217 627 1329 239 1455 1842
Fragments arranged by size:
1842 1455 1329 627 325 239 217
BanII G_rGCy'C
Cuts at: 1391 2103 2349 2484 2638 2958 3227 3353 1391
Size: 712 246 135 154 320 269 126 4072
Fragments arranged by size:
4072 712 320 269 246 154 135 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5063
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2387 2421 4225 4259 1903
Size: 34 50 34 366 34 1804 34 3678
Fragments arranged by size:
3678 1804 366 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3926 5136 3926
Size: 1210 4824
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6034
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2337 2694 4645
Size: 107 122 71 631 605 665 357 1951
Cuts at: 4645 136
Size: 1525
Fragments arranged by size:
1951 1525 665 631 605 357 122 107
71
BglII A'GATC_T
Cuts at: 6029 6029
Size: 6034
BlpI GC'TnA_GC
Cuts at: 3451 3451
Size: 6034
Bme1580I G_kGCm'C
Cuts at: 1790 2389 2453 2484 2638 3039 3075 3227 4095
Size: 599 64 31 154 401 36 152 868
Cuts at: 4095 5251 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 868 599 401 154 152 64
36 31
BmgBI CAC'GTC
Cuts at: 1318 3082 1318
Size: 1764 4270
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3765 4723 442
Size: 416 53 83 399 24 2348 958 1753
Fragments arranged by size:
2348 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2737 3269 4694 1239
Size: 1498 532 1425 2579
Fragments arranged by size:
2579 1498 1425 532
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4159 5024 5322 4159
Size: 865 298 4871
Fragments arranged by size:
4871 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2040 2974 4697 882
Size: 617 541 934 1723 2219
Fragments arranged by size:
2219 1723 934 617 541
BsaAI yAC'GTr
Cuts at: 493 1204 3238 3825 493
Size: 711 2034 587 2702
Fragments arranged by size:
2702 2034 711 587
BsaBI GATnn'nnATC
Cuts at: 6028 6028
Size: 6034
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3841 4223 4858 275
Size: 53 83 186 936 2308 382 635 1451
Fragments arranged by size:
2308 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4467 4865 5139 5286 5697 4467
Size: 398 274 147 411 4804
Fragments arranged by size:
4804 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2585 2615 2870 2900 1178
Size: 30 165 30 1182 30 255 30 4312
Fragments arranged by size:
4312 1182 255 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2073 1186
Size: 887 5147
BseYI C'CCAG_C
Cuts at: 2411 2584 5237 5733 2411
Size: 173 2653 496 2712
Fragments arranged by size:
2712 2653 496 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 1163
Size: 573 645 4816
Fragments arranged by size:
4816 645 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3215 3484 4245 5273 1540
Size: 275 186 1214 269 761 1028 2301
Fragments arranged by size:
2301 1214 1028 761 275 269 186
BsiHKAI G_wGCw'C
Cuts at: 2453 2888 3039 4095 4180 5251 2453
Size: 435 151 1056 85 1071 3236
Fragments arranged by size:
3236 1071 1056 435 151 85
BsmI GAATG_Cn'
Cuts at: 2123 3545 3638 2123
Size: 1422 93 4519
Fragments arranged by size:
4519 1422 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2349 2389 2453 2484 2638 2888
Size: 399 313 246 40 64 31 154 250
Cuts at: 2888 2958 3039 3075 3227 3353 4095 4180 5251
Size: 70 81 36 152 126 742 85 1071
Cuts at: 5251 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 313 250 246 154
152 126 85 81 70 64 40 36
31
BspEI T'CCGG_A
Cuts at: 5697 5697
Size: 6034
BspHI T'CATG_A
Cuts at: 3273 3923 5653 3273
Size: 650 1730 3654
Fragments arranged by size:
3654 1730 650
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2462 2550 3389 844
Size: 1618 88 839 3489
Fragments arranged by size:
3489 1618 839 88
BsrBI CCG'CTC
Cuts at: 1394 1823 2253 2312 2601 3064 3921 1394
Size: 429 430 59 289 463 857 3507
Fragments arranged by size:
3507 857 463 430 429 289 59
BsrDI GCAATG_nn'
Cuts at: 65 1248 4529 4703 65
Size: 1183 3281 174 1396
Fragments arranged by size:
3281 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3214 4678 4865 1700
Size: 110 1404 1464 187 2869
Fragments arranged by size:
2869 1464 1404 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6034
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6034
BssSI C'ACGA_G
Cuts at: 4094 5106 4094
Size: 1012 5022
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6034
BstBI TT'CG_AA
Cuts at: 4838 4838
Size: 6034
BstEII G'GTnAC_C
Cuts at: 1184 2465 2819 3256 1184
Size: 1281 354 437 3962
Fragments arranged by size:
3962 1281 437 354
BstYI r'GATC_y
Cuts at: 1972 3090 3135 3364 3731 4116 4133 5585 5671
Size: 1118 45 229 367 385 17 1452 86
Cuts at: 5671 5683 6029 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1118 385 367 346 229 86
45 17 12
Bsu36I CC'TnA_GG
Cuts at: 2085 2190 2085
Size: 105 5929
BtgI C'CryG_G
Cuts at: 513 1065 2914 513
Size: 552 1849 3633
Fragments arranged by size:
3633 1849 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5295
Fragments arranged by size:
5295 683 56
BtsI GCAGTG_nn'
Cuts at: 2155 2734 2806 3550 4344 4364 2155
Size: 579 72 744 794 20 3825
Fragments arranged by size:
3825 794 744 579 72 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5999
DraI TTT'AAA
Cuts at: 3338 3338
Size: 6034
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5041 809
Size: 507 3725 1802
Fragments arranged by size:
3725 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2832 3212 3481
Size: 54 1425 50 275 18 1002 380 269
Cuts at: 3481 4370 5999 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1002 889 380 275 269 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3212 3481 1537
Size: 275 1400 269 4090
Fragments arranged by size:
4090 1400 275 269
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 3964 1353
Size: 738 1873 3423
Fragments arranged by size:
3423 1873 738
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4621 4995 5141 387
Size: 1064 138 3032 374 146 1280
Fragments arranged by size:
3032 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2737 3269 4097 4694 5481 5749
Size: 393 483 622 532 828 597 787 268
Cuts at: 5749 1239
Size: 1524
Fragments arranged by size:
1524 828 787 622 597 532 483 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2140 2288 2392 2588 2634 2635 2670 3224 3321
Size: 148 104 196 46 1 35 554 97
Cuts at: 3321 2140
Size: 4853
Fragments arranged by size:
4853 554 196 148 104 97 46 35
1
EcoRI G'AATT_C
Cuts at: 3265 3343 3721 3265
Size: 78 378 5578
Fragments arranged by size:
5578 378 78
FspI TGC'GCA
Cuts at: 2877 4540 2877
Size: 1663 4371
FspAI rTGC'GCAy
Cuts at: 2877 2877
Size: 6034
HaeII r_GCGC'y
Cuts at: 1678 2054 2653 3195 5181 1678
Size: 376 599 542 1986 2531
Fragments arranged by size:
2531 1986 599 542 376
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2705 2737 2852 2884 4675
Size: 32 135 32 1133 32 115 32 1791
Cuts at: 4675 4707 4749 4781 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1791 1133 135 115 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2170 2782 2908 3378 3624 669
Size: 988 281 232 612 126 470 246 3079
Fragments arranged by size:
3079 988 612 470 281 246 232 126
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6034
HpaI GTT'AAC
Cuts at: 3624 3624
Size: 6034
KpnI G_GTAC'C
Cuts at: 3359 3359
Size: 6034
MfeI C'AATT_G
Cuts at: 3633 3633
Size: 6034
MluI A'CGCG_T
Cuts at: 5910 5910
Size: 6034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3024 5148 5332 1305
Size: 1719 2124 184 2007
Fragments arranged by size:
2124 2007 1719 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2834 10
Size: 54 1425 343 1002 3210
Fragments arranged by size:
3210 1425 1002 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2712 3992 4351 4510 518
Size: 648 1546 1280 359 159 2042
Fragments arranged by size:
2042 1546 1280 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2258 2364 2532 2559 2584 2775 2827 2928
Size: 652 106 168 27 25 191 52 101
Cuts at: 2928 2946 2982 3077 3450 4127 5275 5520 1606
Size: 18 36 95 373 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 652 373 245 191 168
106 101 95 52 36 27 25 18
NcoI C'CATG_G
Cuts at: 513 1065 2914 513
Size: 552 1849 3633
Fragments arranged by size:
3633 1849 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6034
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6034
NotI GC'GGCC_GC
Cuts at: 3481 3481
Size: 6034
NspI r_CATG'y
Cuts at: 1132 1229 3392 4937 1132
Size: 97 2163 1545 2229
Fragments arranged by size:
2229 2163 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6034
PciI A'CATG_T
Cuts at: 4933 4933
Size: 6034
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2830 1108
Size: 1722 4312
PmeI GTTT'AAAC
Cuts at: 3338 3338
Size: 6034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4103 4135 5616 5648 4103
Size: 32 1481 32 4489
Fragments arranged by size:
4489 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2140 2288 2670 2140
Size: 148 382 5504
Fragments arranged by size:
5504 382 148
PshAI GACnn'nnGTC
Cuts at: 1613 3104 1613
Size: 1491 4543
PsiI TTA'TAA
Cuts at: 3604 3604
Size: 6034
PspOMI G'GGCC_C
Cuts at: 2480 2634 3223 2480
Size: 154 589 5291
Fragments arranged by size:
5291 589 154
PstI C_TGCA'G
Cuts at: 830 2729 3386 830
Size: 1899 657 3478
Fragments arranged by size:
3478 1899 657
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6034
PvuII CAG'CTG
Cuts at: 2364 2584 2827 2364
Size: 220 243 5571
Fragments arranged by size:
5571 243 220
SalI G'TCGA_C
Cuts at: 1936 3376 1936
Size: 1440 4594
SanDI GG'GwC_CC
Cuts at: 2140 2288 2140
Size: 148 5886
SapI GCTCTTCn'nnn_
Cuts at: 2091 2091
Size: 6034
SbfI CC_TGCA'GG
Cuts at: 3386 3386
Size: 6034
ScaI AGT'ACT
Cuts at: 4282 5994 4282
Size: 1712 4322
SfcI C'TryA_G
Cuts at: 826 1045 2725 3382 4517 5198 5389 826
Size: 219 1680 657 1135 681 191 1471
Fragments arranged by size:
1680 1471 1135 681 657 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6034
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6034
SmaI CCC'GGG
Cuts at: 1872 2032 3230 3361 1872
Size: 160 1198 131 4545
Fragments arranged by size:
4545 1198 160 131
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4138 5039 5301 820
Size: 197 926 2195 901 262 1553
Fragments arranged by size:
2195 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3825 493
Size: 3332 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6034
SphI G_CATG'C
Cuts at: 1132 3392 1132
Size: 2260 3774
SspI AAT'ATT
Cuts at: 5 52 3958 5
Size: 47 3906 2081
Fragments arranged by size:
3906 2081 47
StuI AGG'CCT
Cuts at: 1517 2195 1517
Size: 678 5356
StyI C'CwwG_G
Cuts at: 513 1065 2914 3473 513
Size: 552 1849 559 3074
Fragments arranged by size:
3074 1849 559 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4061 4078 4231 4416 4861 325
Size: 1255 2481 17 153 185 445 1498
Fragments arranged by size:
2481 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3295 4280 5992
Size: 274 80 33 51 1443 1318 985 1712
Cuts at: 5992 96
Size: 138
Fragments arranged by size:
1712 1443 1318 985 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2198 2230 2870 2902 5994 6026 2198
Size: 32 640 32 3092 32 2206
Fragments arranged by size:
3092 2206 640 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6034
XbaI T'CTAG_A
Cuts at: 3370 3370
Size: 6034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2432 2947 2432
Size: 515 5519
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6034
XmaI C'CCGG_G
Cuts at: 1870 2030 3228 3359 1870
Size: 160 1198 131 4545
Fragments arranged by size:
4545 1198 160 131
XmnI GAAnn'nnTTC
Cuts at: 4163 4163
Size: 6034
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3842 276
Size: 53 83 186 936 2308 2468
Fragments arranged by size:
2468 2308 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SexAI SfiI SfoI SrfI SwaI