Restriction map for
pFN21AB6639
(Circular) (Six-base) MAPSORT of: pFN21AB6639.seq Check: 1131 from: 1 to: 5959
pFN21AB6639 5959 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2944 3004 3769 278
Size: 53 83 186 936 1408 60 765 2468
Fragments arranged by size:
2468 1408 936 765 186 83 60 53
AccI GT'mk_AC
Cuts at: 1937 2051 2771 3302 1937
Size: 114 720 531 4594
Fragments arranged by size:
4594 720 531 114
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2340 2568 4022 5406 5674 1632
Size: 708 228 1454 1384 268 1917
Fragments arranged by size:
1917 1454 1384 708 268 228
AfeI AGC'GCT
Cuts at: 2693 2693
Size: 5959
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 2495 4790 2495
Size: 2295 3664
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3144 1184
Size: 1960 3999
AlwNI CAG_nnn'CTG
Cuts at: 2318 2623 3702 5274 2318
Size: 305 1079 1572 3003
Fragments arranged by size:
3003 1572 1079 305
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2726 3268 3494 3646 5755 1493
Size: 1233 542 226 152 2109 1697
Fragments arranged by size:
2109 1697 1233 542 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3284 1870
Size: 73 1341 4545
Fragments arranged by size:
4545 1341 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3280 4735 618
Size: 325 217 627 1493 1455 1842
Fragments arranged by size:
1842 1493 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3278 1391
Size: 1887 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2328 928
Size: 971 429 4559
Fragments arranged by size:
4559 971 429
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 2137 1548
Size: 589 5370
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 4020 5176 1790
Size: 2230 1156 2573
Fragments arranged by size:
2573 2230 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2346 2637 2991 4619 1239
Size: 1107 291 354 1628 2579
Fragments arranged by size:
2579 1628 1107 354 291
Bpu10I CC'TnA_GC
Cuts at: 2449 2676 2449
Size: 227 5732
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2452 4084 4949 5247 2452
Size: 1632 865 298 3164
Fragments arranged by size:
3164 1632 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2625 4622 882
Size: 617 1126 1997 2219
Fragments arranged by size:
2219 1997 1126 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2941 3001 3766 4148
Size: 53 83 186 936 1408 60 765 382
Cuts at: 4148 4783 275
Size: 635 1451
Fragments arranged by size:
1451 1408 936 765 635 382 186 83
60 53
BsaWI w'CCGG_w
Cuts at: 2495 4392 4790 5064 5211 5622 2495
Size: 1897 398 274 147 411 2832
Fragments arranged by size:
2832 1897 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2332 2362 2508 2538 1178
Size: 30 165 30 929 30 146 30 4599
Fragments arranged by size:
4599 929 165 146 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5959
BseYI C'CCAG_C
Cuts at: 5162 5658 5162
Size: 496 5463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2453 1163
Size: 573 717 4669
Fragments arranged by size:
4669 717 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4020 4105 5176 4020
Size: 85 1071 4803
Fragments arranged by size:
4803 1071 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3278 4020 4105 5176 1391
Size: 399 1488 742 85 1071 2174
Fragments arranged by size:
2174 1488 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3314 844
Size: 2470 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2424 4454 4628 65
Size: 1183 1176 2030 174 1396
Fragments arranged by size:
2030 1396 1183 1176 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2020 2495 4603 4790 1700
Size: 110 210 475 2108 187 2869
Fragments arranged by size:
2869 2108 475 210 187 110
BsrGI T'GTAC_A
Cuts at: 96 2421 3097 96
Size: 2325 676 2958
Fragments arranged by size:
2958 2325 676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 3289 3656 4041 4058 5510 5596 5608 5954
Size: 1317 367 385 17 1452 86 12 346
Cuts at: 5954 1972
Size: 1977
Fragments arranged by size:
1977 1452 1317 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2052 2772 2052
Size: 720 5239
BtgI C'CryG_G
Cuts at: 513 1065 2004 2242 513
Size: 552 939 238 4230
Fragments arranged by size:
4230 939 552 238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 3475 4269 4289 3475
Size: 794 20 5145
Fragments arranged by size:
5145 794 20
ClaI AT'CG_AT
Cuts at: 1554 2811 1554
Size: 1257 4702
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 2299 3263 2299
Size: 964 4995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2231 3406 4295
Size: 54 1425 50 275 18 401 1175 889
Cuts at: 4295 5924 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1175 889 401 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3889 1353
Size: 2536 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2228 4546 4920 5066 387
Size: 1064 138 639 2318 374 146 1280
Fragments arranged by size:
2318 1280 1064 639 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2340 2346 2568 2637 2991 4022 4619
Size: 393 708 6 222 69 354 1031 597
Cuts at: 4619 5406 5674 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1031 787 708 597 393 354 268
222 69 6
EcoO109I rG'GnC_Cy
Cuts at: 2837 2837
Size: 5959
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2695 5106 1678
Size: 1017 2411 2531
Fragments arranged by size:
2531 2411 1017
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2332 2364 2508 2540 2829
Size: 32 135 32 760 32 144 32 289
Cuts at: 2829 2861 2931 2963 4600 4632 4674 4706 1373
Size: 32 70 32 1637 32 42 32 2626
Fragments arranged by size:
2626 1637 760 289 144 135 70 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2493 3303 3549 669
Size: 988 281 555 810 246 3079
Fragments arranged by size:
3079 988 810 555 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5073 5257 1305
Size: 3768 184 2007
Fragments arranged by size:
3768 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2233 10
Size: 54 1425 343 401 3736
Fragments arranged by size:
3736 1425 401 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2429 2540 3917 4276 4435 518
Size: 648 1263 111 1377 359 159 2042
Fragments arranged by size:
2042 1377 1263 648 359 159 111
MspA1I CmG'CkG
Cuts at: 1606 2568 3375 4052 5200 5445 1606
Size: 962 807 677 1148 245 2120
Fragments arranged by size:
2120 1148 962 807 677 245
NaeI GCC'GGC
Cuts at: 2022 2022
Size: 5959
NcoI C'CATG_G
Cuts at: 513 1065 2004 2242 513
Size: 552 939 238 4230
Fragments arranged by size:
4230 939 552 238
NdeI CA'TA_TG
Cuts at: 387 2218 387
Size: 1831 4128
NgoMIV G'CCGG_C
Cuts at: 2020 2020
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3121 3317 4862 1132
Size: 97 1892 196 1545 2229
Fragments arranged by size:
2229 1892 1545 196 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3245 1108
Size: 2137 3822
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2837 2837
Size: 5959
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 3311 830
Size: 2481 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2568 2568
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2695 3030 3307 4442 5123 5314 826
Size: 219 1650 335 277 1135 681 191 1471
Fragments arranged by size:
1650 1471 1135 681 335 277 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2467 4063 4964 5226 820
Size: 197 926 524 1596 901 262 1553
Fragments arranged by size:
1596 1553 926 901 524 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 2303 2959 3883 5
Size: 47 2251 656 924 2081
Fragments arranged by size:
2251 2081 924 656 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2004 2242 2664 3398 513
Size: 552 939 238 422 734 3074
Fragments arranged by size:
3074 939 734 552 422 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 2649 3986 4003 4156 4341 4786
Size: 1255 543 526 1337 17 153 185 445
Cuts at: 4786 325
Size: 1498
Fragments arranged by size:
1498 1337 1255 543 526 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 3097 4205
Size: 274 80 33 51 1443 444 676 1108
Cuts at: 4205 5917 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1108 676 444 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5919 5951 5919
Size: 32 5927
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 2913 4088 2913
Size: 1175 4784
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2942 3002 3767 276
Size: 53 83 186 936 1408 60 765 2468
Fragments arranged by size:
2468 1408 936 765 186 83 60 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI