Restriction map for
pFN21AB6636
(Circular) (Six-base) MAPSORT of: pFN21AB6636.seq Check: 8898 from: 1 to: 6028
pFN21AB6636 6028 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3838 278
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3371 1937
Size: 1434 4594
Acc65I G'GTAC_C
Cuts at: 3349 3349
Size: 6028
AclI AA'CG_TT
Cuts at: 1563 4155 4528 1563
Size: 2592 373 3063
Fragments arranged by size:
3063 2592 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2390 2469 2561 2658 3027 4091 5475 5743
Size: 758 79 92 97 369 1064 1384 268
Cuts at: 5743 1632
Size: 1917
Fragments arranged by size:
1917 1384 1064 758 369 268 97 92
79
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5831
AflIII A'CryG_T
Cuts at: 1203 4927 5904 1203
Size: 3724 977 1327
Fragments arranged by size:
3724 1327 977
AgeI A'CCGG_T
Cuts at: 4859 4859
Size: 6028
AhdI GACnn_n'nnGTC
Cuts at: 4757 4757
Size: 6028
AleI CACnn'nnGTG
Cuts at: 1166 2740 2742 1166
Size: 1574 2 4452
Fragments arranged by size:
4452 1574 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6028
AlwNI CAG_nnn'CTG
Cuts at: 2117 3771 5343 2117
Size: 1654 1572 2802
Fragments arranged by size:
2802 1654 1572
ApaLI G'TGCA_C
Cuts at: 3276 4085 5241 3276
Size: 809 1156 4063
Fragments arranged by size:
4063 1156 809
ApoI r'AATT_y
Cuts at: 1493 2415 2986 3066 3337 3563 3715 5824 1493
Size: 922 571 80 271 226 152 2109 1697
Fragments arranged by size:
2109 1697 922 571 271 226 152 80
AseI AT'TA_AT
Cuts at: 160 1032 4582 160
Size: 872 3550 1606
Fragments arranged by size:
3550 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
AvaI C'yCGr_G
Cuts at: 1870 1943 3353 1870
Size: 73 1410 4545
Fragments arranged by size:
4545 1410 73
BamHI G'GATC_C
Cuts at: 2374 3358 3725 2374
Size: 984 367 4677
Fragments arranged by size:
4677 984 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3349 4804 618
Size: 325 217 627 1562 1455 1842
Fragments arranged by size:
1842 1562 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3347 1391
Size: 1956 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2610 2664 3032 928
Size: 971 711 54 368 3924
Fragments arranged by size:
3924 971 711 368 54
BbvCI CC'TCA_GC
Cuts at: 2633 2633
Size: 6028
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4219 4253 1903
Size: 34 2282 34 3678
Fragments arranged by size:
3678 2282 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2926 3920 5130 2926
Size: 994 1210 3824
Fragments arranged by size:
3824 1210 994
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6028
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4639 136
Size: 107 122 71 631 605 2967 1525
Fragments arranged by size:
2967 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6023 6023
Size: 6028
BlpI GC'TnA_GC
Cuts at: 2763 3445 2763
Size: 682 5346
Bme1580I G_kGCm'C
Cuts at: 1790 3280 4089 5245 1790
Size: 1490 809 1156 2573
Fragments arranged by size:
2573 1490 1156 809
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6028
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3759 4717 442
Size: 416 53 83 399 24 2342 958 1753
Fragments arranged by size:
2342 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2139 2400 2447 4688 1239
Size: 900 261 47 2241 2579
Fragments arranged by size:
2579 2241 900 261 47
Bpu10I CC'TnA_GC
Cuts at: 2633 2633
Size: 6028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2832 4153 5018 5316 2832
Size: 1321 865 298 3544
Fragments arranged by size:
3544 1321 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4691 882
Size: 617 3192 2219
Fragments arranged by size:
3192 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3819 493
Size: 711 2615 2702
Fragments arranged by size:
2702 2615 711
BsaBI GATnn'nnATC
Cuts at: 6022 6022
Size: 6028
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3835 4217 4852 275
Size: 53 83 186 936 2302 382 635 1451
Fragments arranged by size:
2302 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4461 4859 5133 5280 5691 4461
Size: 398 274 147 411 4798
Fragments arranged by size:
4798 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2065 2095 2373 2403 1178
Size: 30 165 30 662 30 278 30 4803
Fragments arranged by size:
4803 662 278 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2762 1186
Size: 1576 4452
BseYI C'CCAG_C
Cuts at: 5231 5727 5231
Size: 496 5532
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5455
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3478 4239 5267 1540
Size: 275 186 1477 761 1028 2301
Fragments arranged by size:
2301 1477 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3280 4089 4174 5245 3280
Size: 809 85 1071 4063
Fragments arranged by size:
4063 1071 809 85
BsmI GAATG_Cn'
Cuts at: 3539 3632 3539
Size: 93 5935
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3280 3347 4089 4174 5245 1391
Size: 399 1490 67 742 85 1071 2174
Fragments arranged by size:
2174 1490 1071 742 399 85 67
BspEI T'CCGG_A
Cuts at: 5691 5691
Size: 6028
BspHI T'CATG_A
Cuts at: 3917 5647 3917
Size: 1730 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3383 844
Size: 2539 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3915 1394
Size: 429 2092 3507
Fragments arranged by size:
3507 2092 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2949 4523 4697 65
Size: 1183 1701 1574 174 1396
Fragments arranged by size:
1701 1574 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4672 4859 1700
Size: 110 2862 187 2869
Fragments arranged by size:
2869 2862 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6028
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6028
BssSI C'ACGA_G
Cuts at: 4088 5100 4088
Size: 1012 5016
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6028
BstBI TT'CG_AA
Cuts at: 4832 4832
Size: 6028
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6028
BstYI r'GATC_y
Cuts at: 1972 2374 3358 3725 4110 4127 5579 5665 5677
Size: 402 984 367 385 17 1452 86 12
Cuts at: 5677 6023 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 984 402 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4537
Fragments arranged by size:
4537 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5289
Fragments arranged by size:
5289 683 56
BtsI GCAGTG_nn'
Cuts at: 3174 3544 4338 4358 3174
Size: 370 794 20 4844
Fragments arranged by size:
4844 794 370 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5993
DraI TTT'AAA
Cuts at: 2961 3332 2961
Size: 371 5657
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5035 809
Size: 507 3719 1802
Fragments arranged by size:
3719 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3475 4364 5993
Size: 54 1425 50 275 18 1645 889 1629
Cuts at: 5993 8
Size: 43
Fragments arranged by size:
1645 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3475 1537
Size: 275 1663 4090
Fragments arranged by size:
4090 1663 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2176 2197 3126 3156 3958 1353
Size: 823 21 929 30 802 3423
Fragments arranged by size:
3423 929 823 802 30 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3224 4615 4989 5135 387
Size: 1064 138 1635 1391 374 146 1280
Fragments arranged by size:
1635 1391 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2139 2390 2400 2447 2469 2561 2658
Size: 393 507 251 10 47 22 92 97
Cuts at: 2658 3027 4091 4688 5475 5743 1239
Size: 369 1064 597 787 268 1524
Fragments arranged by size:
1524 1064 787 597 507 393 369 268
251 97 92 47 22 10
EcoRI G'AATT_C
Cuts at: 3337 3715 3337
Size: 378 5650
EcoRV GAT'ATC
Cuts at: 2302 2302
Size: 6028
FspI TGC'GCA
Cuts at: 4534 4534
Size: 6028
HaeII r_GCGC'y
Cuts at: 1678 5175 1678
Size: 3497 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2468 2500 4669 4701 4743
Size: 32 135 32 896 32 2169 32 42
Cuts at: 4743 4775 1373
Size: 32 2626
Fragments arranged by size:
2626 2169 896 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3319 3372 3618 669
Size: 988 281 1381 53 246 3079
Fragments arranged by size:
3079 1381 988 281 246 53
HindIII A'AGCT_T
Cuts at: 748 2829 2927 748
Size: 2081 98 3849
Fragments arranged by size:
3849 2081 98
HpaI GTT'AAC
Cuts at: 3618 3618
Size: 6028
KpnI G_GTAC'C
Cuts at: 3353 3353
Size: 6028
MfeI C'AATT_G
Cuts at: 2349 2721 3627 2349
Size: 372 906 4750
Fragments arranged by size:
4750 906 372
MluI A'CGCG_T
Cuts at: 5904 5904
Size: 6028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5142 5326 1305
Size: 3837 184 2007
Fragments arranged by size:
3837 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4206
Fragments arranged by size:
4206 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2642 2740 2742 3986 4345 4504 518
Size: 648 1476 98 2 1244 359 159 2042
Fragments arranged by size:
2042 1476 1244 648 359 159 98 2
MspA1I CmG'CkG
Cuts at: 1606 2921 3444 4121 5269 5514 1606
Size: 1315 523 677 1148 245 2120
Fragments arranged by size:
2120 1315 1148 677 523 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4537
Fragments arranged by size:
4537 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6028
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6028
NotI GC'GGCC_GC
Cuts at: 3475 3475
Size: 6028
NspI r_CATG'y
Cuts at: 1132 1229 2231 3005 3038 3386 4931 1132
Size: 97 1002 774 33 348 1545 2229
Fragments arranged by size:
2229 1545 1002 774 348 97 33
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6028
PciI A'CATG_T
Cuts at: 4927 4927
Size: 6028
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6028
PfoI T'CCnGG_A
Cuts at: 2377 2377
Size: 6028
PmeI GTTT'AAAC
Cuts at: 3332 3332
Size: 6028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3252 3284 4097 4129 5610 5642 3252
Size: 32 813 32 1481 32 3638
Fragments arranged by size:
3638 1481 813 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6028
PsiI TTA'TAA
Cuts at: 3598 3598
Size: 6028
PstI C_TGCA'G
Cuts at: 830 2113 2670 3380 830
Size: 1283 557 710 3478
Fragments arranged by size:
3478 1283 710 557
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6028
SalI G'TCGA_C
Cuts at: 1936 3370 1936
Size: 1434 4594
SapI GCTCTTCn'nnn_
Cuts at: 3126 3126
Size: 6028
SbfI CC_TGCA'GG
Cuts at: 3380 3380
Size: 6028
ScaI AGT'ACT
Cuts at: 4276 5988 4276
Size: 1712 4316
SfcI C'TryA_G
Cuts at: 826 1045 2109 2666 3376 4511 5192 5383 826
Size: 219 1064 557 710 1135 681 191 1471
Fragments arranged by size:
1471 1135 1064 710 681 557 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6028
SmaI CCC'GGG
Cuts at: 1872 3355 1872
Size: 1483 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2847 4132 5033 5295 820
Size: 197 926 904 1285 901 262 1553
Fragments arranged by size:
1553 1285 926 904 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3819 493
Size: 3326 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6028
SphI G_CATG'C
Cuts at: 1132 3005 3386 1132
Size: 1873 381 3774
Fragments arranged by size:
3774 1873 381
SspI AAT'ATT
Cuts at: 5 52 2865 3952 5
Size: 47 2813 1087 2081
Fragments arranged by size:
2813 2081 1087 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6028
StyI C'CwwG_G
Cuts at: 513 1065 2004 2093 3467 513
Size: 552 939 89 1374 3074
Fragments arranged by size:
3074 1374 939 552 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4055 4072 4225 4410 4855 325
Size: 1255 2475 17 153 185 445 1498
Fragments arranged by size:
2475 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2152 2709 4274
Size: 274 80 33 51 1443 175 557 1565
Cuts at: 4274 5986 96
Size: 1712 138
Fragments arranged by size:
1712 1565 1443 557 274 175 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2159 2191 5988 6020 2159
Size: 32 3797 32 2167
Fragments arranged by size:
3797 2167 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6028
XbaI T'CTAG_A
Cuts at: 3364 3364
Size: 6028
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6028
XmaI C'CCGG_G
Cuts at: 1870 3353 1870
Size: 1483 4545
XmnI GAAnn'nnTTC
Cuts at: 4157 4157
Size: 6028
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3836 276
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI