Restriction map for
pFN21AB6628
(Circular) (Six-base) MAPSORT of: pFN21AB6628.seq Check: 7341 from: 1 to: 5923
pFN21AB6628 5923 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2981 2981
Size: 5923
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2278 2896 3266 1937
Size: 341 618 370 4594
Fragments arranged by size:
4594 618 370 341
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2748 3986 5370 5638 1632
Size: 1116 1238 1384 268 1917
Fragments arranged by size:
1917 1384 1238 1116 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2439 1184
Size: 1255 4668
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 5891
AlwNI CAG_nnn'CTG
Cuts at: 2974 3666 5238 2974
Size: 692 1572 3659
Fragments arranged by size:
3659 1572 692
ApaI G_GGCC'C
Cuts at: 2863 3130 2863
Size: 267 5656
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 2430 2540 3232 3458 3610 5719 1493
Size: 937 110 692 226 152 2109 1697
Fragments arranged by size:
2109 1697 937 692 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2439 3248 1870
Size: 73 496 809 4545
Fragments arranged by size:
4545 809 496 73
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3244 4699 618
Size: 325 217 627 1457 1455 1842
Fragments arranged by size:
1842 1457 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2757 2863 3130 3242 1391
Size: 1366 106 267 112 4072
Fragments arranged by size:
4072 1366 267 112 106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2736 928
Size: 971 837 4115
Fragments arranged by size:
4115 971 837
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2149 2183 4114 4148 1903
Size: 34 212 34 1931 34 3678
Fragments arranged by size:
3678 1931 212 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2858 3086 4534
Size: 107 122 71 631 605 1186 228 1448
Cuts at: 4534 136
Size: 1525
Fragments arranged by size:
1525 1448 1186 631 605 228 122 107
71
BglII A'GATC_T
Cuts at: 2443 5918 2443
Size: 3475 2448
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 2854 2863 3130 3984 5140 1790
Size: 1064 9 267 854 1156 2573
Fragments arranged by size:
2573 1156 1064 854 267 9
BmgBI CAC'GTC
Cuts at: 1318 2513 1318
Size: 1195 4728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2364 2592 3175
Size: 416 53 83 399 24 947 228 583
Cuts at: 3175 3654 4612 442
Size: 479 958 1753
Fragments arranged by size:
1753 958 947 583 479 416 399 228
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 3181 4583 1239
Size: 1229 713 1402 2579
Fragments arranged by size:
2579 1402 1229 713
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2141 4586 882
Size: 617 642 2445 2219
Fragments arranged by size:
2445 2219 642 617
BsaAI yAC'GTr
Cuts at: 493 1204 2489 2536 3714 493
Size: 711 1285 47 1178 2702
Fragments arranged by size:
2702 1285 1178 711 47
BsaBI GATnn'nnATC
Cuts at: 2199 5917 2199
Size: 3718 2205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3730 4112 4747 275
Size: 53 83 186 936 2197 382 635 1451
Fragments arranged by size:
2197 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4356 4754 5028 5175 5586 4356
Size: 398 274 147 411 4693
Fragments arranged by size:
4693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2206 2218 2236 2248 3161
Size: 30 165 30 803 12 18 12 913
Cuts at: 3161 3191 1178
Size: 30 3910
Fragments arranged by size:
3910 913 803 165 30 30 30 18
12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 1186
Size: 1213 4710
BseYI C'CCAG_C
Cuts at: 2268 2596 5126 5622 2268
Size: 328 2530 496 2569
Fragments arranged by size:
2569 2530 496 328
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2696 1163
Size: 573 960 4390
Fragments arranged by size:
4390 960 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3984 4069 5140 3984
Size: 85 1071 4767
Fragments arranged by size:
4767 1071 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2757 2854 2863 3130 3242 3984 4069
Size: 399 967 97 9 267 112 742 85
Cuts at: 4069 5140 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 967 742 399 267 112 97
85 9
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2673 2981 3278 844
Size: 1829 308 297 3489
Fragments arranged by size:
3489 1829 308 297
BsrBI CCG'CTC
Cuts at: 1394 1823 2808 3810 1394
Size: 429 985 1002 3507
Fragments arranged by size:
3507 1002 985 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2149 2526 2683 4418 4592 65
Size: 1183 901 377 157 1735 174 1396
Fragments arranged by size:
1735 1396 1183 901 377 174 157
BsrFI r'CCGG_y
Cuts at: 1700 1810 4567 4754 1700
Size: 110 2757 187 2869
Fragments arranged by size:
2869 2757 187 110
BsrGI T'GTAC_A
Cuts at: 96 2614 96
Size: 2518 3405
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2593 794
Size: 1799 4124
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2443 2977 3253 3620 4005 4022 5474 5560
Size: 471 534 276 367 385 17 1452 86
Cuts at: 5560 5572 5918 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 534 471 385 367 346 276
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2382 2382
Size: 5923
BtgI C'CryG_G
Cuts at: 513 1065 2004 2652 3219 513
Size: 552 939 648 567 3217
Fragments arranged by size:
3217 939 648 567 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2496 487
Size: 683 56 1270 3914
Fragments arranged by size:
3914 1270 683 56
BtsI GCAGTG_nn'
Cuts at: 2252 3051 3439 4233 4253 2252
Size: 799 388 794 20 3922
Fragments arranged by size:
3922 799 794 388 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2568 2655 2911
Size: 54 1425 50 275 18 738 87 256
Cuts at: 2911 3370 4259 5888 8
Size: 459 889 1629 43
Fragments arranged by size:
1629 1425 889 738 459 275 256 87
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2061 3853 1353
Size: 708 1792 3423
Fragments arranged by size:
3423 1792 708
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2468 2748 3181 3986 4583 5370 5638
Size: 393 836 280 433 805 597 787 268
Cuts at: 5638 1239
Size: 1524
Fragments arranged by size:
1524 836 805 787 597 433 393 280
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2669 2786 2669
Size: 117 5806
EcoO109I rG'GnC_Cy
Cuts at: 2860 3126 3127 2860
Size: 266 1 5656
Fragments arranged by size:
5656 266 1
EcoRI G'AATT_C
Cuts at: 2430 2540 3232 3610 2430
Size: 110 692 378 4743
Fragments arranged by size:
4743 692 378 110
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 3033 5070 1678
Size: 1355 2037 2531
Fragments arranged by size:
2531 2037 1355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2167 2199 2206 2218 2238
Size: 32 135 32 595 32 7 12 20
Cuts at: 2238 2250 2444 2476 2492 2524 2592 2624 2969
Size: 12 194 32 16 32 68 32 345
Cuts at: 2969 3001 4564 4596 4638 4670 1373
Size: 32 1563 32 42 32 2626
Fragments arranged by size:
2626 1563 595 345 194 135 68 42
32 32 32 32 32 32 32 32
32 20 16 12 12 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2279 2897 3267 3513 669
Size: 988 281 341 618 370 246 3079
Fragments arranged by size:
3079 988 618 370 341 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2204 3064 5037 5221 1305
Size: 899 860 1973 184 2007
Fragments arranged by size:
2007 1973 899 860 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2570 2657 2913 10
Size: 54 1425 343 738 87 256 3020
Fragments arranged by size:
3020 1425 738 343 256 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2286 2313 3881 4240 4399 518
Size: 648 1120 27 1568 359 159 2042
Fragments arranged by size:
2042 1568 1120 648 359 159 27
MspA1I CmG'CkG
Cuts at: 1606 2590 3339 4016 5164 5409 1606
Size: 984 749 677 1148 245 2120
Fragments arranged by size:
2120 1148 984 749 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2652 3219 513
Size: 552 939 648 567 3217
Fragments arranged by size:
3217 939 648 567 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NruI TCG'CGA
Cuts at: 2296 2481 2296
Size: 185 5738
NspI r_CATG'y
Cuts at: 1132 1229 2737 3281 4826 1132
Size: 97 1508 544 1545 2229
Fragments arranged by size:
2229 1545 1508 544 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2974 3220 1108
Size: 1866 246 3811
Fragments arranged by size:
3811 1866 246
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 3992 4024 5505 5537 3161
Size: 32 799 32 1481 32 3547
Fragments arranged by size:
3547 1481 799 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2310 1613
Size: 697 5226
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PspOMI G'GGCC_C
Cuts at: 2859 3126 2859
Size: 267 5656
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2460 2492 2460
Size: 32 5891
PstI C_TGCA'G
Cuts at: 830 3275 830
Size: 2445 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 2277 2895 3265 1936
Size: 341 618 370 4594
Fragments arranged by size:
4594 618 370 341
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 2466 3271 4406 5087 5278 826
Size: 219 1421 805 1135 681 191 1471
Fragments arranged by size:
1471 1421 1135 805 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2439 4027 4928 5190 820
Size: 197 926 496 1588 901 262 1553
Fragments arranged by size:
1588 1553 926 901 496 262 197
SnaBI TAC'GTA
Cuts at: 493 2489 3714 493
Size: 1996 1225 2702
Fragments arranged by size:
2702 1996 1225
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 2776 3847 5
Size: 47 2724 1071 2081
Fragments arranged by size:
2724 2081 1071 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2004 2652 3120 3219 3362 513
Size: 552 939 648 468 99 143 3074
Fragments arranged by size:
3074 939 648 552 468 143 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3950 3967 4120 4305 4750 325
Size: 1255 2370 17 153 185 445 1498
Fragments arranged by size:
2370 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2614 4169 5881
Size: 274 80 33 51 1443 637 1555 1712
Cuts at: 5881 96
Size: 138
Fragments arranged by size:
1712 1555 1443 637 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5883 5915 5883
Size: 32 5891
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 2439 1943
Size: 496 5427
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI
PacI PfoI PmlI PpuMI PspXI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI