Restriction map for
pFN21AB6627
(Circular) (Six-base) MAPSORT of: pFN21AB6627.seq Check: 4713 from: 1 to: 5950
pFN21AB6627 5950 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2130 2472 2610 2925 3293 1937
Size: 193 342 138 315 368 4594
Fragments arranged by size:
4594 368 342 315 193 138
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2162 2583 2928 3081 3126 4013 5397 5665
Size: 530 421 345 153 45 887 1384 268
Cuts at: 5665 1632
Size: 1917
Fragments arranged by size:
1917 1384 887 530 421 345 268 153
45
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 4849 5826 1203
Size: 3646 977 1327
Fragments arranged by size:
3646 1327 977
AgeI A'CCGG_T
Cuts at: 4781 4781
Size: 5950
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5950
AlwNI CAG_nnn'CTG
Cuts at: 3116 3693 5265 3116
Size: 577 1572 3801
Fragments arranged by size:
3801 1572 577
ApaLI G'TGCA_C
Cuts at: 4007 5163 4007
Size: 1156 4794
ApoI r'AATT_y
Cuts at: 1493 3259 3485 3637 5746 1493
Size: 1766 226 152 2109 1697
Fragments arranged by size:
2109 1766 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2511 4504 160
Size: 872 1479 1993 1606
Fragments arranged by size:
1993 1606 1479 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 3275 1870
Size: 73 1332 4545
Fragments arranged by size:
4545 1332 73
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3271 4726 618
Size: 325 217 627 1484 1455 1842
Fragments arranged by size:
1842 1484 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2141 3269 1391
Size: 750 1128 4072
Fragments arranged by size:
4072 1128 750
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2163 2751 928
Size: 971 264 588 4127
Fragments arranged by size:
4127 971 588 264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4141 4175 1903
Size: 34 2204 34 3678
Fragments arranged by size:
3678 2204 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2575 2729 3842 5052 2575
Size: 154 1113 1210 3473
Fragments arranged by size:
3473 1210 1113 154
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5950
BfrBI ATG'CAT
Cuts at: 2351 3202 2351
Size: 851 5099
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4561 136
Size: 107 122 71 631 605 2889 1525
Fragments arranged by size:
2889 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 2685 3367 2685
Size: 682 5268
Bme1580I G_kGCm'C
Cuts at: 1790 3104 4011 5167 1790
Size: 1314 907 1156 2573
Fragments arranged by size:
2573 1314 1156 907
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5950
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2929 3681 4639
Size: 416 53 83 399 24 1512 752 958
Cuts at: 4639 442
Size: 1753
Fragments arranged by size:
1753 1512 958 752 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2125 2205 2802 4610 1239
Size: 886 80 597 1808 2579
Fragments arranged by size:
2579 1808 886 597 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4613 882
Size: 617 3114 2219
Fragments arranged by size:
3114 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3741 493
Size: 711 2537 2702
Fragments arranged by size:
2702 2537 711
BsaBI GATnn'nnATC
Cuts at: 5944 5944
Size: 5950
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2997 3757 4139 4774
Size: 53 83 186 936 1464 760 382 635
Cuts at: 4774 275
Size: 1451
Fragments arranged by size:
1464 1451 936 760 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4383 4781 5055 5202 5613 4383
Size: 398 274 147 411 4720
Fragments arranged by size:
4720 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2123 2153 1178
Size: 30 165 30 720 30 4975
Fragments arranged by size:
4975 720 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2443 3110 1186
Size: 1257 667 4026
Fragments arranged by size:
4026 1257 667
BseYI C'CCAG_C
Cuts at: 5153 5649 5153
Size: 496 5454
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2508 1163
Size: 573 772 4605
Fragments arranged by size:
4605 772 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3400 4161 5189 1540
Size: 275 186 1399 761 1028 2301
Fragments arranged by size:
2301 1399 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4011 4096 5167 4011
Size: 85 1071 4794
Fragments arranged by size:
4794 1071 85
BsmI GAATG_Cn'
Cuts at: 2608 3461 3554 2608
Size: 853 93 5004
Fragments arranged by size:
5004 853 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 3104 3269 4011 4096 5167 1391
Size: 399 351 963 165 742 85 1071 2174
Fragments arranged by size:
2174 1071 963 742 399 351 165 85
BspEI T'CCGG_A
Cuts at: 5613 5613
Size: 5950
BspHI T'CATG_A
Cuts at: 3839 5569 3839
Size: 1730 4220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3305 844
Size: 2461 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2328 3837 1394
Size: 429 505 1509 3507
Fragments arranged by size:
3507 1509 505 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4445 4619 65
Size: 1183 3197 174 1396
Fragments arranged by size:
3197 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4594 4781 1700
Size: 110 2784 187 2869
Fragments arranged by size:
2869 2784 187 110
BsrGI T'GTAC_A
Cuts at: 96 2698 96
Size: 2602 3348
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2300 2348 794
Size: 1506 48 4396
Fragments arranged by size:
4396 1506 48
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5950
BstXI CCAn_nnnn'nTGG
Cuts at: 2125 2839 2125
Size: 714 5236
BstYI r'GATC_y
Cuts at: 1972 2011 2256 2966 3280 3647 4032 4049 5501
Size: 39 245 710 314 367 385 17 1452
Cuts at: 5501 5587 5599 5945 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 710 385 367 346 314 245
86 39 17 12
BstZ17I GTA'TAC
Cuts at: 2926 2926
Size: 5950
BtgI C'CryG_G
Cuts at: 513 1065 2004 2099 2118 2124 3078 513
Size: 552 939 95 19 6 954 3385
Fragments arranged by size:
3385 954 939 552 95 19 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5211
Fragments arranged by size:
5211 683 56
BtsI GCAGTG_nn'
Cuts at: 3466 4260 4280 3466
Size: 794 20 5136
Fragments arranged by size:
5136 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2166 3397
Size: 54 1425 50 275 18 291 45 1231
Cuts at: 3397 4286 5915 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1231 889 291 275 54 50
45 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2943 3880 1353
Size: 740 850 937 3423
Fragments arranged by size:
3423 937 850 740
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4537 4911 5057 387
Size: 1064 138 2948 374 146 1280
Fragments arranged by size:
2948 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2125 2162 2205 2583 2802 2928 3081
Size: 393 493 37 43 378 219 126 153
Cuts at: 3081 3126 4013 4610 5397 5665 1239
Size: 45 887 597 787 268 1524
Fragments arranged by size:
1524 887 787 597 493 393 378 268
219 153 126 45 43 37
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FspI TGC'GCA
Cuts at: 4456 4456
Size: 5950
HaeII r_GCGC'y
Cuts at: 1678 5097 1678
Size: 3419 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4591 4623 4665 4697 1373
Size: 32 135 32 3019 32 42 32 2626
Fragments arranged by size:
3019 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3294 3540 669
Size: 988 281 1356 246 3079
Fragments arranged by size:
3079 1356 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3015 5064 5248 1305
Size: 1710 2049 184 2007
Fragments arranged by size:
2049 2007 1710 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2123 2168 10
Size: 54 1425 343 291 45 3792
Fragments arranged by size:
3792 1425 343 291 54 45
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 2987 3908 4267 4426 518
Size: 648 957 864 921 359 159 2042
Fragments arranged by size:
2042 957 921 864 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2238 3116 3366 4043 5191 5436 1606
Size: 632 878 250 677 1148 245 2120
Fragments arranged by size:
2120 1148 878 677 632 250 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2099 2118 2124 3078 513
Size: 552 939 95 19 6 954 3385
Fragments arranged by size:
3385 954 939 552 95 19 6
NdeI CA'TA_TG
Cuts at: 387 2776 387
Size: 2389 3561
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NsiI A_TGCA'T
Cuts at: 2353 3204 2353
Size: 851 5099
NspI r_CATG'y
Cuts at: 1132 1229 2797 3308 4853 1132
Size: 97 1568 511 1545 2229
Fragments arranged by size:
2229 1568 1545 511 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2780 3079 1108
Size: 1672 299 3979
Fragments arranged by size:
3979 1672 299
PfoI T'CCnGG_A
Cuts at: 2415 2415
Size: 5950
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4019 4051 5532 5564 4019
Size: 32 1481 32 4405
Fragments arranged by size:
4405 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PstI C_TGCA'G
Cuts at: 830 2305 3302 830
Size: 1475 997 3478
Fragments arranged by size:
3478 1475 997
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
PvuII CAG'CTG
Cuts at: 2238 2238
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SapI GCTCTTCn'nnn_
Cuts at: 2943 2943
Size: 5950
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SfcI C'TryA_G
Cuts at: 826 1045 2301 2571 3298 4433 5114 5305 826
Size: 219 1256 270 727 1135 681 191 1471
Fragments arranged by size:
1471 1256 1135 727 681 270 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5950
SmaI CCC'GGG
Cuts at: 1872 3277 1872
Size: 1405 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4054 4955 5217 820
Size: 197 926 2111 901 262 1553
Fragments arranged by size:
2111 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 2447 3874 5
Size: 47 2395 1427 2081
Fragments arranged by size:
2395 2081 1427 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5950
StyI C'CwwG_G
Cuts at: 513 1065 2004 2099 2118 2124 2169 3078 3389
Size: 552 939 95 19 6 45 909 311
Cuts at: 3389 513
Size: 3074
Fragments arranged by size:
3074 939 909 552 311 95 45 19
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2093 3977 3994 4147 4332 4777 325
Size: 1255 513 1884 17 153 185 445 1498
Fragments arranged by size:
1884 1498 1255 513 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2698 2709 4196
Size: 274 80 33 51 1443 721 11 1487
Cuts at: 4196 5908 96
Size: 1712 138
Fragments arranged by size:
1712 1487 1443 721 274 138 80 51
33 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5910 5942 5910
Size: 32 5918
Tth111I GACn'n_nGTC
Cuts at: 1316 2436 1316
Size: 1120 4830
XbaI T'CTAG_A
Cuts at: 2764 3286 2764
Size: 522 5428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2122 2131 2593 2122
Size: 9 462 5479
Fragments arranged by size:
5479 462 9
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 3275 1870
Size: 1405 4545
XmnI GAAnn'nnTTC
Cuts at: 2087 2427 4079 2087
Size: 340 1652 3958
Fragments arranged by size:
3958 1652 340
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI