Restriction map for
pFN21AB6619
(Circular) (Six-base) MAPSORT of: pFN21AB6619.seq Check: 4233 from: 1 to: 5941
pFN21AB6619 5941 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3043 3043
Size: 5941
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2097 3751 278
Size: 53 83 186 936 561 1654 2468
Fragments arranged by size:
2468 1654 936 561 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2969 3284 1937
Size: 1032 315 4594
Fragments arranged by size:
4594 1032 315
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2180 3221 4004 5388 5656 1632
Size: 464 84 1041 783 1384 268 1917
Fragments arranged by size:
1917 1384 1041 783 464 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 2460 2972 4772 2460
Size: 512 1800 3629
Fragments arranged by size:
3629 1800 512
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3155 1184
Size: 1971 3970
AlwNI CAG_nnn'CTG
Cuts at: 2292 3684 5256 2292
Size: 1392 1572 2977
Fragments arranged by size:
2977 1572 1392
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 2450 2951 2999 3250 3476 3628 5737 1493
Size: 957 501 48 251 226 152 2109 1697
Fragments arranged by size:
2109 1697 957 501 251 226 152 48
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2672 2838 3266 1870
Size: 73 729 166 428 4545
Fragments arranged by size:
4545 729 428 166 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2273 2354 2726 2864 3262
Size: 325 217 627 486 81 372 138 398
Cuts at: 3262 4717 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 486 398 372 325 217
138 81
BanII G_rGCy'C
Cuts at: 1391 2394 2754 3260 1391
Size: 1003 360 506 4072
Fragments arranged by size:
4072 1003 506 360
BbeI G_GCGC'C
Cuts at: 2358 2358
Size: 5941
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2136 2636 928
Size: 971 237 500 4233
Fragments arranged by size:
4233 971 500 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2108 4552 136
Size: 107 122 71 631 605 436 2444 1525
Fragments arranged by size:
2444 1525 631 605 436 122 107 71
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 2393 2958 3358 2393
Size: 565 400 4976
Fragments arranged by size:
4976 565 400
Bme1580I G_kGCm'C
Cuts at: 1790 2276 4002 5158 1790
Size: 486 1726 1156 2573
Fragments arranged by size:
2573 1726 1156 486
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3672 4630 442
Size: 416 53 83 399 24 2255 958 1753
Fragments arranged by size:
2255 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2458 2598 2609 3203 4601 1239
Size: 1219 140 11 594 1398 2579
Fragments arranged by size:
2579 1398 1219 594 140 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3050 4066 4931 5229 3050
Size: 1016 865 298 3762
Fragments arranged by size:
3762 1016 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2624 2846 4604 882
Size: 617 1125 222 1758 2219
Fragments arranged by size:
2219 1758 1125 617 222
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 2513 5935 2513
Size: 3422 2519
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2094 2355 3748 4130
Size: 53 83 186 936 561 261 1393 382
Cuts at: 4130 4765 275
Size: 635 1451
Fragments arranged by size:
1451 1393 936 635 561 382 261 186
83 53
BsaWI w'CCGG_w
Cuts at: 2382 2460 2972 4374 4772 5046 5193 5604 2382
Size: 78 512 1402 398 274 147 411 2719
Fragments arranged by size:
2719 1402 512 411 398 274 147 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2726 2756 1178
Size: 30 165 30 1323 30 4363
Fragments arranged by size:
4363 1323 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 3134 1186
Size: 1042 906 3993
Fragments arranged by size:
3993 1042 906
BseYI C'CCAG_C
Cuts at: 2322 2641 2857 5144 5640 2322
Size: 319 216 2287 496 2623
Fragments arranged by size:
2623 2287 496 319 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3071 3161 1163
Size: 573 1335 90 3943
Fragments arranged by size:
3943 1335 573 90
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3391 4152 5180 1540
Size: 275 186 1090 300 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 300 275 186
BsiHKAI G_wGCw'C
Cuts at: 2394 2754 4002 4087 5158 2394
Size: 360 1248 85 1071 3177
Fragments arranged by size:
3177 1248 1071 360 85
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
BsmBI CGTCTCn'nnnn_
Cuts at: 3221 3221
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2276 2394 2754 3260 4002 4087 5158
Size: 399 486 118 360 506 742 85 1071
Cuts at: 5158 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 506 486 399 360 118
85
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3043 3296 844
Size: 2199 253 3489
Fragments arranged by size:
3489 2199 253
BsrBI CCG'CTC
Cuts at: 1394 1823 3828 1394
Size: 429 2005 3507
Fragments arranged by size:
3507 2005 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2097 2427 2482 4436 4610 65
Size: 1183 849 330 55 1954 174 1396
Fragments arranged by size:
1954 1396 1183 849 330 174 55
BsrFI r'CCGG_y
Cuts at: 1700 1810 2227 2460 2972 4585 4772 1700
Size: 110 417 233 512 1613 187 2869
Fragments arranged by size:
2869 1613 512 417 233 187 110
BsrGI T'GTAC_A
Cuts at: 96 3078 96
Size: 2982 2959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 2056 2066 4001 5013 2056
Size: 10 1935 1012 2984
Fragments arranged by size:
2984 1935 1012 10
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5941
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 2707 1184
Size: 1523 4418
BstYI r'GATC_y
Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936
Size: 1299 367 385 17 1452 86 12 346
Cuts at: 5936 1972
Size: 1977
Fragments arranged by size:
1977 1452 1299 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2970 2970
Size: 5941
Bsu36I CC'TnA_GG
Cuts at: 3194 3194
Size: 5941
BtgI C'CryG_G
Cuts at: 513 1065 2022 2350 2537 2907 513
Size: 552 957 328 187 370 3547
Fragments arranged by size:
3547 957 552 370 328 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2281 3001 487
Size: 683 56 1055 720 3427
Fragments arranged by size:
3427 1055 720 683 56
BtsI GCAGTG_nn'
Cuts at: 3010 3231 3457 4251 4271 3010
Size: 221 226 794 20 4680
Fragments arranged by size:
4680 794 226 221 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DraIII CAC_nnn'GTG
Cuts at: 2058 2058
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2119 2225
Size: 54 1425 50 275 18 279 10 106
Cuts at: 2225 2229 3088 3388 4277 5906 8
Size: 4 859 300 889 1629 43
Fragments arranged by size:
1629 1425 889 859 300 279 275 106
54 50 43 18 10 4
EagI C'GGCC_G
Cuts at: 1537 1812 3088 3388 1537
Size: 275 1276 300 4090
Fragments arranged by size:
4090 1276 300 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2759 3871 1353
Size: 1406 1112 3423
Fragments arranged by size:
3423 1406 1112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2996 4528 4902 5048 387
Size: 1064 138 1407 1532 374 146 1280
Fragments arranged by size:
1532 1407 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2392 2752 2392
Size: 360 5581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2180 2458 2598 2609 3203 3221
Size: 393 464 84 278 140 11 594 18
Cuts at: 3221 4004 4601 5388 5656 1239
Size: 783 597 787 268 1524
Fragments arranged by size:
1524 787 783 597 594 464 393 278
268 140 84 18 11
EcoO109I rG'GnC_Cy
Cuts at: 2289 2638 2695 2289
Size: 349 57 5535
Fragments arranged by size:
5535 349 57
EcoRI G'AATT_C
Cuts at: 2450 3250 3628 2450
Size: 800 378 4763
Fragments arranged by size:
4763 800 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 5941
FseI GG_CCGG'CC
Cuts at: 2231 2231
Size: 5941
FspI TGC'GCA
Cuts at: 2924 4447 2924
Size: 1523 4418
HaeII r_GCGC'y
Cuts at: 1678 2358 2612 5088 1678
Size: 680 254 2476 2531
Fragments arranged by size:
2531 2476 680 254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373
Size: 32 135 32 3010 32 42 32 2626
Fragments arranged by size:
3010 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2162 748
Size: 1414 4527
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KasI G'GCGC_C
Cuts at: 2354 2354
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5055 5239 1305
Size: 3750 184 2007
Fragments arranged by size:
3750 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2111 2121 10
Size: 54 1425 343 279 10 3830
Fragments arranged by size:
3830 1425 343 279 54 10
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 2828 3137 3899 4258 4417 518
Size: 648 900 762 309 762 359 159 2042
Fragments arranged by size:
2042 900 762 762 648 359 309 159
MspA1I CmG'CkG
Cuts at: 1606 2326 2397 3357 4034 5182 5427 1606
Size: 720 71 960 677 1148 245 2120
Fragments arranged by size:
2120 1148 960 720 677 245 71
NaeI GCC'GGC
Cuts at: 2229 2229
Size: 5941
NarI GG'CG_CC
Cuts at: 2355 2355
Size: 5941
NcoI C'CATG_G
Cuts at: 513 1065 2907 513
Size: 552 1842 3547
Fragments arranged by size:
3547 1842 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NgoMIV G'CCGG_C
Cuts at: 2227 2227
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3299 4844 1132
Size: 97 2070 1545 2229
Fragments arranged by size:
2229 2070 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5941
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5941
PfoI T'CCnGG_A
Cuts at: 2586 2586
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2666 2698 4010 4042 5523 5555 2666
Size: 32 1312 32 1481 32 3052
Fragments arranged by size:
3052 1481 1312 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2638 2695 2638
Size: 57 5884
PshAI GACnn'nnGTC
Cuts at: 1613 2714 1613
Size: 1101 4840
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PstI C_TGCA'G
Cuts at: 830 3293 830
Size: 2463 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2397 2397
Size: 5941
SacI G_AGCT'C
Cuts at: 2394 2754 2394
Size: 360 5581
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SanDI GG'GwC_CC
Cuts at: 2695 2695
Size: 5941
SapI GCTCTTCn'nnn_
Cuts at: 2759 2759
Size: 5941
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SfcI C'TryA_G
Cuts at: 826 1045 2035 3289 4424 5105 5296 826
Size: 219 990 1254 1135 681 191 1471
Fragments arranged by size:
1471 1254 1135 990 681 219 191
SfoI GGC'GCC
Cuts at: 2356 2356
Size: 5941
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 3268 1872
Size: 1396 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2672 3065 4045 4946 5208 820
Size: 197 926 729 393 980 901 262 1553
Fragments arranged by size:
1553 980 926 901 729 393 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 2900 2940 1517
Size: 1383 40 4518
Fragments arranged by size:
4518 1383 40
StyI C'CwwG_G
Cuts at: 513 1065 2745 2907 3380 513
Size: 552 1680 162 473 3074
Fragments arranged by size:
3074 1680 552 473 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2747 3968 3985 4138 4323 4768 325
Size: 1255 1167 1221 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1221 1167 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3078 4187 5899
Size: 274 80 33 51 1443 1101 1109 1712
Cuts at: 5899 96
Size: 138
Fragments arranged by size:
1712 1443 1109 1101 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 2726 2758 5901 5933 2042
Size: 32 652 32 3143 32 2050
Fragments arranged by size:
3143 2050 652 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XhoI C'TCGA_G
Cuts at: 1943 2672 1943
Size: 729 5212
XmaI C'CCGG_G
Cuts at: 1870 3266 1870
Size: 1396 4545
XmnI GAAnn'nnTTC
Cuts at: 2819 4070 2819
Size: 1251 4690
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2095 3749 276
Size: 53 83 186 936 561 1654 2468
Fragments arranged by size:
2468 1654 936 561 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI EcoNI EcoRV FspAI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SexAI SfiI SrfI SwaI XcmI