Restriction map for
pFN21AB6618
(Circular) (Six-base) MAPSORT of: pFN21AB6618.seq Check: 3153 from: 1 to: 6004
pFN21AB6618 6004 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2488 2488
Size: 6004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3347 1937
Size: 1410 4594
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 3303 4131 4504 1563
Size: 1740 828 373 3063
Fragments arranged by size:
3063 1740 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4067 5451 5719 1632
Size: 2435 1384 268 1917
Fragments arranged by size:
2435 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 4903 5880 1203
Size: 3700 977 1327
Fragments arranged by size:
3700 1327 977
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 3130 1166
Size: 1964 4040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AlwNI CAG_nnn'CTG
Cuts at: 3179 3747 5319 3179
Size: 568 1572 3864
Fragments arranged by size:
3864 1572 568
ApaLI G'TGCA_C
Cuts at: 4061 5217 4061
Size: 1156 4848
ApoI r'AATT_y
Cuts at: 1493 2448 3029 3269 3313 3539 3691 5800 1493
Size: 955 581 240 44 226 152 2109 1697
Fragments arranged by size:
2109 1697 955 581 240 226 152 44
AseI AT'TA_AT
Cuts at: 160 1032 2181 4558 160
Size: 872 1149 2377 1606
Fragments arranged by size:
2377 1606 1149 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 2937 3329 1870
Size: 73 994 392 4545
Fragments arranged by size:
4545 994 392 73
BamHI G'GATC_C
Cuts at: 2986 3334 3701 2986
Size: 348 367 5289
Fragments arranged by size:
5289 367 348
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2392 3325 4780 618
Size: 325 217 627 234 371 933 1455 1842
Fragments arranged by size:
1842 1455 933 627 371 325 234 217
BanII G_rGCy'C
Cuts at: 1391 2077 2190 2767 2854 3323 1391
Size: 686 113 577 87 469 4072
Fragments arranged by size:
4072 686 577 469 113 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2042 928
Size: 971 143 4890
Fragments arranged by size:
4890 971 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4195 4229 1903
Size: 34 50 34 2174 34 3678
Fragments arranged by size:
3678 2174 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 2383 2910 1548
Size: 835 527 4642
Fragments arranged by size:
4642 835 527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4615 136
Size: 107 122 71 631 605 2943 1525
Fragments arranged by size:
2943 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5999 5999
Size: 6004
BlpI GC'TnA_GC
Cuts at: 3421 3421
Size: 6004
Bme1580I G_kGCm'C
Cuts at: 1790 4065 5221 1790
Size: 2275 1156 2573
Fragments arranged by size:
2573 2275 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3735 4693 442
Size: 416 53 83 399 24 2318 958 1753
Fragments arranged by size:
2318 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2892 4664 1239
Size: 1010 643 1772 2579
Fragments arranged by size:
2579 1772 1010 643
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 4129 4994 5292 2156
Size: 1973 865 298 2868
Fragments arranged by size:
2868 1973 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4667 882
Size: 617 3168 2219
Fragments arranged by size:
3168 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3795 493
Size: 711 2591 2702
Fragments arranged by size:
2702 2591 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4437 4835 5109 5256 5667 4437
Size: 398 274 147 411 4774
Fragments arranged by size:
4774 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5779
Fragments arranged by size:
5779 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3273 1186
Size: 2087 3917
BseYI C'CCAG_C
Cuts at: 5207 5703 5207
Size: 496 5508
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2909 3154 1163
Size: 573 1173 245 4013
Fragments arranged by size:
4013 1173 573 245
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2077 2123 2190 3202 4065 4150 5221 2077
Size: 46 67 1012 863 85 1071 2860
Fragments arranged by size:
2860 1071 1012 863 85 67 46
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2123 2190 2767 2854 3202 3323
Size: 399 287 46 67 577 87 348 121
Cuts at: 3323 4065 4150 5221 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 577 399 348 287 121
87 85 67 46
BspEI T'CCGG_A
Cuts at: 5667 5667
Size: 6004
BspHI T'CATG_A
Cuts at: 3893 5623 3893
Size: 1730 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2488 3359 844
Size: 1644 871 3489
Fragments arranged by size:
3489 1644 871
BsrBI CCG'CTC
Cuts at: 1394 1823 3891 1394
Size: 429 2068 3507
Fragments arranged by size:
3507 2068 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2459 4499 4673 65
Size: 1183 1211 2040 174 1396
Fragments arranged by size:
2040 1396 1211 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4648 4835 1700
Size: 110 2838 187 2869
Fragments arranged by size:
2869 2838 187 110
BsrGI T'GTAC_A
Cuts at: 96 2297 2740 96
Size: 2201 443 3360
Fragments arranged by size:
3360 2201 443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6004
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstXI CCAn_nnnn'nTGG
Cuts at: 2235 2368 2235
Size: 133 5871
BstYI r'GATC_y
Cuts at: 1972 2729 2986 3334 3701 4086 4103 5555 5641
Size: 757 257 348 367 385 17 1452 86
Cuts at: 5641 5653 5999 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 757 385 367 348 346 257
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5452
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 3520 4314 4334 3520
Size: 794 20 5190
Fragments arranged by size:
5190 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DraIII CAC_nnn'GTG
Cuts at: 2678 2678
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2793 2961 3451
Size: 54 1425 50 275 18 963 168 490
Cuts at: 3451 4340 5969 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 963 889 490 275 168 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2499 3934 1353
Size: 1146 1435 3423
Fragments arranged by size:
3423 1435 1146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2075 2188 2075
Size: 113 5891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2892 4067 4664 5451 5719 1239
Size: 393 617 643 1175 597 787 268 1524
Fragments arranged by size:
1524 1175 787 643 617 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2326 2326
Size: 6004
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
EcoRV GAT'ATC
Cuts at: 3106 3106
Size: 6004
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2831 3247 2831
Size: 416 5588
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 5151 1678
Size: 3473 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3064 3096 4645 4677 4719
Size: 32 135 32 1492 32 1549 32 42
Cuts at: 4719 4751 1373
Size: 32 2626
Fragments arranged by size:
2626 1549 1492 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3348 3594 669
Size: 988 281 1410 246 3079
Fragments arranged by size:
3079 1410 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2883 3035 5118 5302 1305
Size: 1578 152 2083 184 2007
Fragments arranged by size:
2083 2007 1578 184 152
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2795 2963 10
Size: 54 1425 343 963 168 3051
Fragments arranged by size:
3051 1425 963 343 168 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3130 3962 4321 4480 518
Size: 648 1964 832 359 159 2042
Fragments arranged by size:
2042 1964 832 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 3420 4097 5245 5490 1606
Size: 426 1388 677 1148 245 2120
Fragments arranged by size:
2120 1388 1148 677 426 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5452
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 3362 4907 1132
Size: 97 2133 1545 2229
Fragments arranged by size:
2229 2133 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6004
PciI A'CATG_T
Cuts at: 4903 4903
Size: 6004
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6004
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2326 2326
Size: 6004
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PspXI vC'TCGA_Gb
Cuts at: 2937 2937
Size: 6004
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2265 2297 2265
Size: 32 5972
PstI C_TGCA'G
Cuts at: 830 2251 3356 830
Size: 1421 1105 3478
Fragments arranged by size:
3478 1421 1105
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
SacI G_AGCT'C
Cuts at: 2077 2190 2077
Size: 113 5891
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 4252 5964 4252
Size: 1712 4292
SfcI C'TryA_G
Cuts at: 826 1045 2247 3352 4487 5168 5359 826
Size: 219 1202 1105 1135 681 191 1471
Fragments arranged by size:
1471 1202 1135 1105 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 3331 1872
Size: 1459 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2171 2937 4108 5009 5271 820
Size: 197 926 228 766 1171 901 262 1553
Fragments arranged by size:
1553 1171 926 901 766 262 228 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 2947 3928 5
Size: 47 2895 981 2081
Fragments arranged by size:
2895 2081 981 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6004
StyI C'CwwG_G
Cuts at: 513 1065 2614 2631 3443 513
Size: 552 1549 17 812 3074
Fragments arranged by size:
3074 1549 812 552 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 4048 4201 4386 4831 325
Size: 1255 2451 17 153 185 445 1498
Fragments arranged by size:
2451 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2297 2740 4250
Size: 274 80 33 51 1443 320 443 1510
Cuts at: 4250 5962 96
Size: 1712 138
Fragments arranged by size:
1712 1510 1443 443 320 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5964 5996 5964
Size: 32 5972
Tth111I GACn'n_nGTC
Cuts at: 1316 3132 1316
Size: 1816 4188
XbaI T'CTAG_A
Cuts at: 3046 3340 3046
Size: 294 5710
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 2232 2368 2497 2074
Size: 158 136 129 5581
Fragments arranged by size:
5581 158 136 129
XhoI C'TCGA_G
Cuts at: 1943 2937 1943
Size: 994 5010
XmaI C'CCGG_G
Cuts at: 1870 3329 1870
Size: 1459 4545
XmnI GAAnn'nnTTC
Cuts at: 3227 4133 3227
Size: 906 5098
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI