Restriction map for
pFN21AB6615
(Circular) (Six-base) MAPSORT of: pFN21AB6615.seq Check: 3536 from: 1 to: 5941
pFN21AB6615 5941 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2410 2410
Size: 5941
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3284 1937
Size: 1347 4594
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4004 5388 5656 1632
Size: 2372 1384 268 1917
Fragments arranged by size:
2372 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2432 1184
Size: 1248 4693
AlwNI CAG_nnn'CTG
Cuts at: 2053 2383 3684 5256 2053
Size: 330 1301 1572 2738
Fragments arranged by size:
2738 1572 1301 330
ApaI G_GGCC'C
Cuts at: 2176 3064 2176
Size: 888 5053
ApaLI G'TGCA_C
Cuts at: 3998 5154 3998
Size: 1156 4785
ApoI r'AATT_y
Cuts at: 1493 3250 3476 3628 5737 1493
Size: 1757 226 152 2109 1697
Fragments arranged by size:
2109 1757 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2747 2876 3266 1870
Size: 73 804 129 390 4545
Fragments arranged by size:
4545 804 390 129 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2336 2696 3262 4717 618
Size: 325 217 627 549 360 566 1455 1842
Fragments arranged by size:
1842 1455 627 566 549 360 325 217
BanII G_rGCy'C
Cuts at: 1391 2067 2176 2198 3064 3260 1391
Size: 676 109 22 866 196 4072
Fragments arranged by size:
4072 866 676 196 109 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2899 928
Size: 971 1000 3970
Fragments arranged by size:
3970 1000 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3220 3254 4132 4166 1903
Size: 34 50 34 1199 34 878 34 3678
Fragments arranged by size:
3678 1199 878 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3833 5043 3833
Size: 1210 4731
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2062 2306 2880
Size: 107 122 71 631 605 390 244 574
Cuts at: 2880 3059 3068 4552 136
Size: 179 9 1484 1525
Fragments arranged by size:
1525 1484 631 605 574 390 244 179
122 107 71 9
BglII A'GATC_T
Cuts at: 5936 5936
Size: 5941
BlpI GC'TnA_GC
Cuts at: 2972 3358 2972
Size: 386 5555
Bme1580I G_kGCm'C
Cuts at: 1790 2176 3064 3128 4002 5158 1790
Size: 386 888 64 874 1156 2573
Fragments arranged by size:
2573 1156 888 874 386 64
BmgBI CAC'GTC
Cuts at: 1318 2629 1318
Size: 1311 4630
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2408 3036 3672
Size: 416 53 83 399 24 991 628 636
Cuts at: 3672 4630 442
Size: 958 1753
Fragments arranged by size:
1753 991 958 636 628 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2453 2636 1056
Size: 1397 183 4361
Fragments arranged by size:
4361 1397 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2295 2405 3119 4601 1239
Size: 812 244 110 714 1482 2579
Fragments arranged by size:
2579 1482 812 714 244 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 2974 4066 4931 5229 2144
Size: 830 1092 865 298 2856
Fragments arranged by size:
2856 1092 865 830 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4604 882
Size: 617 3105 2219
Fragments arranged by size:
3105 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3732 493
Size: 711 2528 2702
Fragments arranged by size:
2702 2528 711
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3748 4130 4765 275
Size: 53 83 186 936 2215 382 635 1451
Fragments arranged by size:
2215 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4374 4772 5046 5193 5604 4374
Size: 398 274 147 411 4711
Fragments arranged by size:
4711 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2991 3021 1178
Size: 30 165 30 1588 30 4098
Fragments arranged by size:
4098 1588 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2791 1186
Size: 1605 4336
BseYI C'CCAG_C
Cuts at: 2437 2453 2683 3106 3121 3227 5144 5640 2437
Size: 16 230 423 15 106 1917 496 2738
Fragments arranged by size:
2738 1917 496 423 230 106 16 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2066 2396 2438 2538 1163
Size: 573 330 330 42 100 4566
Fragments arranged by size:
4566 573 330 330 100 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2394 3391 4152 5180 1540
Size: 275 186 393 997 761 1028 2301
Fragments arranged by size:
2301 1028 997 761 393 275 186
BsiHKAI G_wGCw'C
Cuts at: 2673 4002 4087 5158 2673
Size: 1329 85 1071 3456
Fragments arranged by size:
3456 1329 1071 85
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2176 2198 2673 3064 3128 3260
Size: 399 277 109 22 475 391 64 132
Cuts at: 3260 4002 4087 5158 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 475 399 391 277 132
109 85 64 22
BspEI T'CCGG_A
Cuts at: 5604 5604
Size: 5941
BspHI T'CATG_A
Cuts at: 3830 5560 3830
Size: 1730 4211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2410 3296 844
Size: 1566 886 3489
Fragments arranged by size:
3489 1566 886
BsrBI CCG'CTC
Cuts at: 1394 1823 3828 1394
Size: 429 2005 3507
Fragments arranged by size:
3507 2005 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4436 4610 65
Size: 1183 3188 174 1396
Fragments arranged by size:
3188 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 2099 2389 4585 4772 1700
Size: 110 220 69 290 2196 187 2869
Fragments arranged by size:
2869 2196 290 220 187 110 69
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 2460 1925
Size: 535 5406
BssSI C'ACGA_G
Cuts at: 2488 4001 5013 2488
Size: 1513 1012 3416
Fragments arranged by size:
3416 1513 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2053 2620 794
Size: 1259 567 4115
Fragments arranged by size:
4115 1259 567
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 3007 1184
Size: 1823 4118
BstYI r'GATC_y
Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936
Size: 1299 367 385 17 1452 86 12 346
Cuts at: 5936 1972
Size: 1977
Fragments arranged by size:
1977 1452 1299 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2150 2535 2731 513
Size: 552 1085 385 196 3723
Fragments arranged by size:
3723 1085 552 385 196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2776 487
Size: 683 56 1550 3652
Fragments arranged by size:
3652 1550 683 56
BtsI GCAGTG_nn'
Cuts at: 3457 4251 4271 3457
Size: 794 20 5127
Fragments arranged by size:
5127 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 3245 3245
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4948 809
Size: 507 3632 1802
Fragments arranged by size:
3632 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2130 2281
Size: 54 1425 50 275 18 198 102 151
Cuts at: 2281 2391 2492 2532 2823 3090 3132 3388 4277
Size: 110 101 40 291 267 42 256 889
Cuts at: 4277 5906 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 291 275 267 256 198
151 110 102 101 54 50 43 42
40 18
EagI C'GGCC_G
Cuts at: 1537 1812 2391 3388 1537
Size: 275 579 997 4090
Fragments arranged by size:
4090 997 579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2721 3871 1353
Size: 1368 1150 3423
Fragments arranged by size:
3423 1368 1150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2861 4528 4902 5048 387
Size: 1064 138 1272 1667 374 146 1280
Fragments arranged by size:
1667 1280 1272 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2295 2405 3119 4004 4601 5388
Size: 393 419 244 110 714 885 597 787
Cuts at: 5388 5656 1239
Size: 268 1524
Fragments arranged by size:
1524 885 787 714 597 419 393 268
244 110
EcoNI CCTnn'n_nnAGG
Cuts at: 2045 2045
Size: 5941
EcoO109I rG'GnC_Cy
Cuts at: 2050 2173 2205 2814 2881 3060 3061 2050
Size: 123 32 609 67 179 1 4930
Fragments arranged by size:
4930 609 179 123 67 32 1
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FseI GG_CCGG'CC
Cuts at: 2034 2034
Size: 5941
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 2474 5088 1678
Size: 796 2614 2531
Fragments arranged by size:
2614 2531 796
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2965 2991 2997 3023 4582
Size: 32 135 32 1393 26 6 26 1559
Cuts at: 4582 4614 4656 4688 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1559 1393 135 42 32 32 32
32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5941
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5055 5239 1305
Size: 3750 184 2007
Fragments arranged by size:
3750 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2132 2283 2825 3092 10
Size: 54 1425 343 300 151 542 267 2859
Fragments arranged by size:
2859 1425 542 343 300 267 151 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3029 3899 4258 4417 518
Size: 648 1863 870 359 159 2042
Fragments arranged by size:
2042 1863 870 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2179 2303 2380 2537 3049 3357 4034
Size: 420 153 124 77 157 512 308 677
Cuts at: 4034 5182 5427 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 512 420 308 245 157
153 124 77
NaeI GCC'GGC
Cuts at: 2032 2101 2391 2032
Size: 69 290 5582
Fragments arranged by size:
5582 290 69
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5389
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NgoMIV G'CCGG_C
Cuts at: 2030 2099 2389 2030
Size: 69 290 5582
Fragments arranged by size:
5582 290 69
NheI G'CTAG_C
Cuts at: 1052 2449 2632 1052
Size: 1397 183 4361
Fragments arranged by size:
4361 1397 183
NotI GC'GGCC_GC
Cuts at: 3388 3388
Size: 5941
NspI r_CATG'y
Cuts at: 1132 1229 3299 4844 1132
Size: 97 2070 1545 2229
Fragments arranged by size:
2229 2070 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2806 2923 3057 1348
Size: 1458 117 134 4232
Fragments arranged by size:
4232 1458 134 117
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2526 2832 1108
Size: 1418 306 4217
Fragments arranged by size:
4217 1418 306
PfoI T'CCnGG_A
Cuts at: 2272 2272
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2814 2814
Size: 5941
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 2172 3060 2172
Size: 888 5053
PstI C_TGCA'G
Cuts at: 830 2988 3044 3069 3293 830
Size: 2158 56 25 224 3478
Fragments arranged by size:
3478 2158 224 56 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
PvuII CAG'CTG
Cuts at: 2380 3049 2380
Size: 669 5272
SacII CC_GC'GG
Cuts at: 2538 2538
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SbfI CC_TGCA'GG
Cuts at: 3069 3293 3069
Size: 224 5717
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SexAI A'CCwGG_T
Cuts at: 2040 2040
Size: 5941
SfcI C'TryA_G
Cuts at: 826 1045 2984 3040 3065 3079 3289 4424 5105
Size: 219 1939 56 25 14 210 1135 681
Cuts at: 5105 5296 826
Size: 191 1471
Fragments arranged by size:
1939 1471 1135 681 219 210 191 56
25 14
SfiI GGCCn_nnn'nGGCC
Cuts at: 3059 3068 3059
Size: 9 5932
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 3268 1872
Size: 1396 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 2953 4045 4946 5208 820
Size: 197 926 216 794 1092 901 262 1553
Fragments arranged by size:
1553 1092 926 901 794 262 216 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 3003 3141 1517
Size: 1486 138 4317
Fragments arranged by size:
4317 1486 138
StyI C'CwwG_G
Cuts at: 513 1065 2133 2901 3380 513
Size: 552 1068 768 479 3074
Fragments arranged by size:
3074 1068 768 552 479
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2200 2324 2675 2762 2798 3241 3968
Size: 1255 620 124 351 87 36 443 727
Cuts at: 3968 3985 4138 4323 4768 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 727 620 445 443 351 185
153 124 87 36 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4187 5899 96
Size: 274 80 33 51 1443 2210 1712 138
Fragments arranged by size:
2210 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2527 2350
Size: 177 5764
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5941
XmaI C'CCGG_G
Cuts at: 1870 3266 1870
Size: 1396 4545
XmnI GAAnn'nnTTC
Cuts at: 4070 4070
Size: 5941
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SanDI SapI
SfoI SrfI SwaI