Restriction map for
pFN21AB6613
(Circular) (Six-base) MAPSORT of: pFN21AB6613.seq Check: 8043 from: 1 to: 5941
pFN21AB6613 5941 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3751 278
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2271 3284 1937
Size: 334 1013 4594
Fragments arranged by size:
4594 1013 334
Acc65I G'GTAC_C
Cuts at: 3262 3262
Size: 5941
AclI AA'CG_TT
Cuts at: 1563 4068 4441 1563
Size: 2505 373 3063
Fragments arranged by size:
3063 2505 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4004 5388 5656 1632
Size: 2372 1384 268 1917
Fragments arranged by size:
2372 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5744
AflIII A'CryG_T
Cuts at: 1203 4840 5817 1203
Size: 3637 977 1327
Fragments arranged by size:
3637 1327 977
AgeI A'CCGG_T
Cuts at: 4772 4772
Size: 5941
AhdI GACnn_n'nnGTC
Cuts at: 4670 4670
Size: 5941
AleI CACnn'nnGTG
Cuts at: 1166 2036 1166
Size: 870 5071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2054 2453 1184
Size: 870 399 4672
Fragments arranged by size:
4672 870 399
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2954 2986 2954
Size: 32 5909
AlwNI CAG_nnn'CTG
Cuts at: 3684 5256 3684
Size: 1572 4369
ApaI G_GGCC'C
Cuts at: 3138 3138
Size: 5941
ApaLI G'TGCA_C
Cuts at: 2126 3218 3998 5154 2126
Size: 1092 780 1156 2913
Fragments arranged by size:
2913 1156 1092 780
ApoI r'AATT_y
Cuts at: 1493 2111 3250 3476 3628 5737 1493
Size: 618 1139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1139 618 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4495 160
Size: 872 3463 1606
Fragments arranged by size:
3463 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
AvaI C'yCGr_G
Cuts at: 1870 1943 2026 2265 2474 2611 3191 3266 1870
Size: 73 83 239 209 137 580 75 4545
Fragments arranged by size:
4545 580 239 209 137 83 75 73
BamHI G'GATC_C
Cuts at: 3271 3638 3271
Size: 367 5574
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2900 3033 3262 4717
Size: 325 217 627 243 870 133 229 1455
Cuts at: 4717 618
Size: 1842
Fragments arranged by size:
1842 1455 870 627 325 243 229 217
133
BanII G_rGCy'C
Cuts at: 1391 2066 2266 3138 3260 1391
Size: 675 200 872 122 4072
Fragments arranged by size:
4072 872 675 200 122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4970
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4132 4166 1903
Size: 34 2195 34 3678
Fragments arranged by size:
3678 2195 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2410 3833 5043 2410
Size: 1423 1210 3308
Fragments arranged by size:
3308 1423 1210
BclI T'GATC_A
Cuts at: 1548 2659 1548
Size: 1111 4830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4552 136
Size: 107 122 71 631 605 2880 1525
Fragments arranged by size:
2880 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2406 5936 2406
Size: 3530 2411
BlpI GC'TnA_GC
Cuts at: 3358 3358
Size: 5941
Bme1580I G_kGCm'C
Cuts at: 1790 2033 2130 3038 3138 3222 4002 5158 1790
Size: 243 97 908 100 84 780 1156 2573
Fragments arranged by size:
2573 1156 908 780 243 100 97 84
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5941
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2642 3672 4630
Size: 416 53 83 399 24 1225 1030 958
Cuts at: 4630 442
Size: 1753
Fragments arranged by size:
1753 1225 1030 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 5941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2462 2825 4601 1239
Size: 1223 363 1776 2579
Fragments arranged by size:
2579 1776 1223 363
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4066 4931 5229 4066
Size: 865 298 4778
Fragments arranged by size:
4778 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3147 4604 882
Size: 617 1648 1457 2219
Fragments arranged by size:
2219 1648 1457 617
BsaAI yAC'GTr
Cuts at: 493 1204 2206 2641 3732 493
Size: 711 1002 435 1091 2702
Fragments arranged by size:
2702 1091 1002 711 435
BsaBI GATnn'nnATC
Cuts at: 5935 5935
Size: 5941
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3000 3748 4130 4765
Size: 53 83 186 936 1467 748 382 635
Cuts at: 4765 275
Size: 1451
Fragments arranged by size:
1467 1451 936 748 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2505 2826 4374 4772 5046 5193 5604 2505
Size: 321 1548 398 274 147 411 2842
Fragments arranged by size:
2842 1548 411 398 321 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2177 2207 1178
Size: 30 165 30 774 30 4912
Fragments arranged by size:
4912 774 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3119 1186
Size: 1933 4008
BseYI C'CCAG_C
Cuts at: 2978 3037 5144 5640 2978
Size: 59 2107 496 3279
Fragments arranged by size:
3279 2107 496 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2728 3257 1163
Size: 573 992 529 3847
Fragments arranged by size:
3847 992 573 529
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 2827 3095 3391 4152 5180
Size: 275 186 21 805 268 296 761 1028
Cuts at: 5180 1540
Size: 2301
Fragments arranged by size:
2301 1028 805 761 296 275 268 186
21
BsiHKAI G_wGCw'C
Cuts at: 2130 2304 2432 3222 4002 4087 5158 2130
Size: 174 128 790 780 85 1071 2913
Fragments arranged by size:
2913 1071 790 780 174 128 85
BsmI GAATG_Cn'
Cuts at: 3452 3545 3452
Size: 93 5848
BsmBI CGTCTCn'nnnn_
Cuts at: 2729 2729
Size: 5941
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2066 2130 2266 2304 2432 3038
Size: 399 243 33 64 136 38 128 606
Cuts at: 3038 3138 3222 3260 4002 4087 5158 1391
Size: 100 84 38 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 606 399 243 136 128
100 85 84 64 38 38 33
BspEI T'CCGG_A
Cuts at: 2505 5604 2505
Size: 3099 2842
BspHI T'CATG_A
Cuts at: 2335 3830 5560 2335
Size: 1495 1730 2716
Fragments arranged by size:
2716 1730 1495
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 3188 3229 3296 844
Size: 1253 1091 41 67 3489
Fragments arranged by size:
3489 1253 1091 67 41
BsrBI CCG'CTC
Cuts at: 1394 1823 3828 1394
Size: 429 2005 3507
Fragments arranged by size:
3507 2005 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2520 4436 4610 65
Size: 1183 1272 1916 174 1396
Fragments arranged by size:
1916 1396 1272 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4585 4772 1700
Size: 110 2775 187 2869
Fragments arranged by size:
2869 2775 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5941
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5941
BssSI C'ACGA_G
Cuts at: 4001 5013 4001
Size: 1012 4929
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2531 794
Size: 1737 4204
BstBI TT'CG_AA
Cuts at: 4745 4745
Size: 5941
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5941
BstXI CCAn_nnnn'nTGG
Cuts at: 2791 3082 2791
Size: 291 5650
BstYI r'GATC_y
Cuts at: 1972 2406 3271 3638 4023 4040 5492 5578 5590
Size: 434 865 367 385 17 1452 86 12
Cuts at: 5590 5936 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 865 434 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 513
Size: 552 939 30 4420
Fragments arranged by size:
4420 939 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2258 487
Size: 683 56 1032 4170
Fragments arranged by size:
4170 1032 683 56
BtsI GCAGTG_nn'
Cuts at: 3457 4251 4271 3457
Size: 794 20 5127
Fragments arranged by size:
5127 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5941
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5906
DraI TTT'AAA
Cuts at: 2371 3245 2371
Size: 874 5067
DraIII CAC_nnn'GTG
Cuts at: 3033 3033
Size: 5941
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2216 4948 809
Size: 507 900 2732 1802
Fragments arranged by size:
2732 1802 900 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 2052 3388
Size: 54 1425 50 275 18 189 33 1336
Cuts at: 3388 4277 5906 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1336 889 275 189 54 50
43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2019 3388 1537
Size: 275 207 1369 4090
Fragments arranged by size:
4090 1369 275 207
EarI CTCTTCn'nnn_
Cuts at: 1353 2075 2864 3871 1353
Size: 722 789 1007 3423
Fragments arranged by size:
3423 1007 789 722
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4528 4902 5048 387
Size: 1064 138 2939 374 146 1280
Fragments arranged by size:
2939 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2462 2825 4004 4601 5388 5656 1239
Size: 393 830 363 1179 597 787 268 1524
Fragments arranged by size:
1524 1179 830 787 597 393 363 268
EcoRI G'AATT_C
Cuts at: 3250 3628 3250
Size: 378 5563
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2161 2875 2161
Size: 714 5227
FspI TGC'GCA
Cuts at: 4447 4447
Size: 5941
HaeII r_GCGC'y
Cuts at: 1678 5088 1678
Size: 3410 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2708 2740 4582 4614 4656
Size: 32 135 32 1136 32 1842 32 42
Cuts at: 4656 4688 1373
Size: 32 2626
Fragments arranged by size:
2626 1842 1136 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3285 3531 669
Size: 988 281 1347 246 3079
Fragments arranged by size:
3079 1347 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2320 748
Size: 1572 4369
HpaI GTT'AAC
Cuts at: 3531 3531
Size: 5941
KpnI G_GTAC'C
Cuts at: 3266 3266
Size: 5941
MfeI C'AATT_G
Cuts at: 3540 3540
Size: 5941
MluI A'CGCG_T
Cuts at: 5817 5817
Size: 5941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2796 3075 5055 5239 1305
Size: 1491 279 1980 184 2007
Fragments arranged by size:
2007 1980 1491 279 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4119
Fragments arranged by size:
4119 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2435 3059 3213 3899 4258 4417
Size: 648 870 399 624 154 686 359 159
Cuts at: 4417 518
Size: 2042
Fragments arranged by size:
2042 870 686 648 624 399 359 159
154
MspA1I CmG'CkG
Cuts at: 1606 2036 3357 4034 5182 5427 1606
Size: 430 1321 677 1148 245 2120
Fragments arranged by size:
2120 1321 1148 677 430 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4450
Fragments arranged by size:
4450 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5941
NotI GC'GGCC_GC
Cuts at: 2019 3388 2019
Size: 1369 4572
NspI r_CATG'y
Cuts at: 1132 1229 3299 4844 1132
Size: 97 2070 1545 2229
Fragments arranged by size:
2229 2070 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5941
PciI A'CATG_T
Cuts at: 4840 4840
Size: 5941
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5941
PmeI GTTT'AAAC
Cuts at: 3245 3245
Size: 5941
PmlI CAC'GTG
Cuts at: 2206 2206
Size: 5941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 5523 5555 4010
Size: 32 1481 32 4396
Fragments arranged by size:
4396 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5941
PsiI TTA'TAA
Cuts at: 3511 3511
Size: 5941
PspOMI G'GGCC_C
Cuts at: 3134 3134
Size: 5941
PspXI vC'TCGA_Gb
Cuts at: 2265 2265
Size: 5941
PstI C_TGCA'G
Cuts at: 830 2094 3293 830
Size: 1264 1199 3478
Fragments arranged by size:
3478 1264 1199
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5941
RsrII CG'GwC_CG
Cuts at: 2767 2767
Size: 5941
SacII CC_GC'GG
Cuts at: 2037 2037
Size: 5941
SalI G'TCGA_C
Cuts at: 1936 3283 1936
Size: 1347 4594
SapI GCTCTTCn'nnn_
Cuts at: 2075 2075
Size: 5941
SbfI CC_TGCA'GG
Cuts at: 3293 3293
Size: 5941
ScaI AGT'ACT
Cuts at: 4189 5901 4189
Size: 1712 4229
SexAI A'CCwGG_T
Cuts at: 2118 2118
Size: 5941
SfcI C'TryA_G
Cuts at: 826 1045 2090 3289 4424 5105 5296 826
Size: 219 1045 1199 1135 681 191 1471
Fragments arranged by size:
1471 1199 1135 1045 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5941
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5941
SmaI CCC'GGG
Cuts at: 1872 2028 3193 3268 1872
Size: 156 1165 75 4545
Fragments arranged by size:
4545 1165 156 75
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2265 4045 4946 5208 820
Size: 197 926 322 1780 901 262 1553
Fragments arranged by size:
1780 1553 926 901 322 262 197
SnaBI TAC'GTA
Cuts at: 493 3732 493
Size: 3239 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5941
SphI G_CATG'C
Cuts at: 1132 3299 1132
Size: 2167 3774
SspI AAT'ATT
Cuts at: 5 52 3865 5
Size: 47 3813 2081
Fragments arranged by size:
3813 2081 47
StuI AGG'CCT
Cuts at: 1517 2192 1517
Size: 675 5266
StyI C'CwwG_G
Cuts at: 513 1065 2004 3075 3380 513
Size: 552 939 1071 305 3074
Fragments arranged by size:
3074 1071 939 552 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3968 3985 4138 4323 4768 325
Size: 1255 2388 17 153 185 445 1498
Fragments arranged by size:
2388 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2607 3018 4187
Size: 274 80 33 51 1443 630 411 1169
Cuts at: 4187 5899 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1169 630 411 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5901 5933 5901
Size: 32 5909
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5941
XbaI T'CTAG_A
Cuts at: 3277 3277
Size: 5941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2203 2728 3145 2203
Size: 525 417 4999
Fragments arranged by size:
4999 525 417
XhoI C'TCGA_G
Cuts at: 1943 2265 1943
Size: 322 5619
XmaI C'CCGG_G
Cuts at: 1870 2026 3191 3266 1870
Size: 156 1165 75 4545
Fragments arranged by size:
4545 1165 156 75
XmnI GAAnn'nnTTC
Cuts at: 2858 4070 2858
Size: 1212 4729
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3749 276
Size: 53 83 186 936 2215 2468
Fragments arranged by size:
2468 2215 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PsrI PvuII SacI SanDI SfiI
SfoI SrfI SwaI