Restriction map for
pFN21AB6612
(Circular) (Six-base) MAPSORT of: pFN21AB6612.seq Check: 3356 from: 1 to: 6001
pFN21AB6612 6001 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3811 278
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3344 1937
Size: 1407 4594
Acc65I G'GTAC_C
Cuts at: 3322 3322
Size: 6001
AclI AA'CG_TT
Cuts at: 1563 4128 4501 1563
Size: 2565 373 3063
Fragments arranged by size:
3063 2565 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2169 2679 2801 4064 5448 5716 1632
Size: 537 510 122 1263 1384 268 1917
Fragments arranged by size:
1917 1384 1263 537 510 268 122
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5804
AflIII A'CryG_T
Cuts at: 1203 4900 5877 1203
Size: 3697 977 1327
Fragments arranged by size:
3697 1327 977
AgeI A'CCGG_T
Cuts at: 4832 4832
Size: 6001
AhdI GACnn_n'nnGTC
Cuts at: 4730 4730
Size: 6001
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6001
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6001
AlwNI CAG_nnn'CTG
Cuts at: 2473 3158 3744 5316 2473
Size: 685 586 1572 3158
Fragments arranged by size:
3158 1572 685 586
ApaI G_GGCC'C
Cuts at: 2013 2490 2652 2013
Size: 477 162 5362
Fragments arranged by size:
5362 477 162
ApaLI G'TGCA_C
Cuts at: 4058 5214 4058
Size: 1156 4845
ApoI r'AATT_y
Cuts at: 1493 2746 3310 3536 3688 5797 1493
Size: 1253 564 226 152 2109 1697
Fragments arranged by size:
2109 1697 1253 564 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4555 160
Size: 872 3523 1606
Fragments arranged by size:
3523 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
AvaI C'yCGr_G
Cuts at: 1870 1943 2417 2907 3326 1870
Size: 73 474 490 419 4545
Fragments arranged by size:
4545 490 474 419 73
BamHI G'GATC_C
Cuts at: 3331 3698 3331
Size: 367 5634
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3322 4777 618
Size: 325 217 627 1535 1455 1842
Fragments arranged by size:
1842 1535 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2490 2643 2652 3320 1391
Size: 622 477 153 9 668 4072
Fragments arranged by size:
4072 668 622 477 153 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2922 928
Size: 971 1023 4007
Fragments arranged by size:
4007 1023 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4192 4226 1903
Size: 34 2255 34 3678
Fragments arranged by size:
3678 2255 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2425 2747 3893 5103 2425
Size: 322 1146 1210 3323
Fragments arranged by size:
3323 1210 1146 322
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6001
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2028 2607 4612
Size: 107 122 71 631 605 356 579 2005
Cuts at: 4612 136
Size: 1525
Fragments arranged by size:
2005 1525 631 605 579 356 122 107
71
BglII A'GATC_T
Cuts at: 2940 5996 2940
Size: 3056 2945
BlpI GC'TnA_GC
Cuts at: 3418 3418
Size: 6001
Bme1580I G_kGCm'C
Cuts at: 1790 2013 2263 2490 2652 4062 5218 1790
Size: 223 250 227 162 1410 1156 2573
Fragments arranged by size:
2573 1410 1156 250 227 223 162
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6001
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3732 4690 442
Size: 416 53 83 399 24 2315 958 1753
Fragments arranged by size:
2315 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6001
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2612 4661 1239
Size: 1373 2049 2579
Fragments arranged by size:
2579 2049 1373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 4126 4991 5289 2486
Size: 1640 865 298 3198
Fragments arranged by size:
3198 1640 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3236 4664 882
Size: 617 1737 1428 2219
Fragments arranged by size:
2219 1737 1428 617
BsaAI yAC'GTr
Cuts at: 493 1204 3792 493
Size: 711 2588 2702
Fragments arranged by size:
2702 2588 711
BsaBI GATnn'nnATC
Cuts at: 2045 5995 2045
Size: 3950 2051
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3808 4190 4825 275
Size: 53 83 186 936 2275 382 635 1451
Fragments arranged by size:
2275 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3013 4434 4832 5106 5253 5664 3013
Size: 1421 398 274 147 411 3350
Fragments arranged by size:
3350 1421 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2218 2248 2498 2528 1178
Size: 30 165 30 815 30 250 30 4651
Fragments arranged by size:
4651 815 250 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2503 2780 3052 3161 1186
Size: 1317 277 272 109 4026
Fragments arranged by size:
4026 1317 277 272 109
BseYI C'CCAG_C
Cuts at: 2026 2473 3140 5204 5700 2026
Size: 447 667 2064 496 2327
Fragments arranged by size:
2327 2064 667 496 447
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2568 1163
Size: 573 832 4596
Fragments arranged by size:
4596 832 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3451 4212 5240 1540
Size: 275 186 1450 761 1028 2301
Fragments arranged by size:
2301 1450 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2117 2502 2643 4062 4147 5218 2117
Size: 385 141 1419 85 1071 2900
Fragments arranged by size:
2900 1419 1071 385 141 85
BsmI GAATG_Cn'
Cuts at: 3512 3605 3512
Size: 93 5908
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2117 2263 2490 2502 2643 2652
Size: 399 223 104 146 227 12 141 9
Cuts at: 2652 3320 4062 4147 5218 1391
Size: 668 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 668 399 227 223 146
141 104 85 12 9
BspEI T'CCGG_A
Cuts at: 3013 5664 3013
Size: 2651 3350
BspHI T'CATG_A
Cuts at: 3890 5620 3890
Size: 1730 4271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2312 3356 844
Size: 1468 1044 3489
Fragments arranged by size:
3489 1468 1044
BsrBI CCG'CTC
Cuts at: 1394 1823 3888 1394
Size: 429 2065 3507
Fragments arranged by size:
3507 2065 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4496 4670 65
Size: 1183 3248 174 1396
Fragments arranged by size:
3248 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4645 4832 1700
Size: 110 2835 187 2869
Fragments arranged by size:
2869 2835 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6001
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6001
BssSI C'ACGA_G
Cuts at: 4061 5073 4061
Size: 1012 4989
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2231 794
Size: 1437 4564
BstBI TT'CG_AA
Cuts at: 3099 4805 3099
Size: 1706 4295
BstEII G'GTnAC_C
Cuts at: 1184 3054 1184
Size: 1870 4131
BstYI r'GATC_y
Cuts at: 1972 2344 2940 3331 3698 4083 4100 5552 5638
Size: 372 596 391 367 385 17 1452 86
Cuts at: 5638 5650 5996 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 596 391 385 372 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 3226 513
Size: 552 939 9 1213 3288
Fragments arranged by size:
3288 1213 939 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5262
Fragments arranged by size:
5262 683 56
BtsI GCAGTG_nn'
Cuts at: 2251 2433 3517 4311 4331 2251
Size: 182 1084 794 20 3921
Fragments arranged by size:
3921 1084 794 182 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6001
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5966
DraI TTT'AAA
Cuts at: 3305 3305
Size: 6001
DraIII CAC_nnn'GTG
Cuts at: 2055 2055
Size: 6001
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5008 809
Size: 507 3692 1802
Fragments arranged by size:
3692 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3220 3448 4337
Size: 54 1425 50 275 18 1390 228 889
Cuts at: 4337 5966 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1390 889 275 228 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3448 1537
Size: 275 1636 4090
Fragments arranged by size:
4090 1636 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2757 2838 2931 3931 1353
Size: 1404 81 93 1000 3423
Fragments arranged by size:
3423 1404 1000 93 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2855 4588 4962 5108 387
Size: 1064 138 1266 1733 374 146 1280
Fragments arranged by size:
1733 1280 1266 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2641 2641
Size: 6001
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2169 2612 2679 2801 4064 4661 5448
Size: 393 537 443 67 122 1263 597 787
Cuts at: 5448 5716 1239
Size: 268 1524
Fragments arranged by size:
1524 1263 787 597 537 443 393 268
122 67
EcoO109I rG'GnC_Cy
Cuts at: 2009 2486 2487 2648 2649 2770 3043 2009
Size: 477 1 161 1 121 273 4967
Fragments arranged by size:
4967 477 273 161 121 1 1
EcoRI G'AATT_C
Cuts at: 3310 3688 3310
Size: 378 5623
FspI TGC'GCA
Cuts at: 4507 4507
Size: 6001
HaeII r_GCGC'y
Cuts at: 1678 5148 1678
Size: 3470 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2498 2530 3163 3195 4642
Size: 32 135 32 926 32 633 32 1447
Cuts at: 4642 4674 4716 4748 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1447 926 633 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3345 3591 669
Size: 988 281 1407 246 3079
Fragments arranged by size:
3079 1407 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2792 748
Size: 2044 3957
HpaI GTT'AAC
Cuts at: 3591 3591
Size: 6001
KpnI G_GTAC'C
Cuts at: 3326 3326
Size: 6001
MfeI C'AATT_G
Cuts at: 3600 3600
Size: 6001
MluI A'CGCG_T
Cuts at: 5877 5877
Size: 6001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5115 5299 1305
Size: 3810 184 2007
Fragments arranged by size:
3810 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3222 10
Size: 54 1425 343 1390 2789
Fragments arranged by size:
2789 1425 1390 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3959 4318 4477 518
Size: 648 2793 359 159 2042
Fragments arranged by size:
2793 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2015 2034 2582 3417 4094 5242 5487 1606
Size: 409 19 548 835 677 1148 245 2120
Fragments arranged by size:
2120 1148 835 677 548 409 245 19
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4510
Fragments arranged by size:
4510 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6001
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6001
NotI GC'GGCC_GC
Cuts at: 3448 3448
Size: 6001
NspI r_CATG'y
Cuts at: 1132 1229 2311 3359 4904 1132
Size: 97 1082 1048 1545 2229
Fragments arranged by size:
2229 1545 1082 1048 97
PasI CC'CwG_GG
Cuts at: 1348 2645 3208 1348
Size: 1297 563 4141
Fragments arranged by size:
4141 1297 563
PciI A'CATG_T
Cuts at: 4900 4900
Size: 6001
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2473 1108
Size: 1365 4636
PmeI GTTT'AAAC
Cuts at: 3305 3305
Size: 6001
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 5583 5615 4070
Size: 32 1481 32 4456
Fragments arranged by size:
4456 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3043 3043
Size: 6001
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6001
PsiI TTA'TAA
Cuts at: 3571 3571
Size: 6001
PspOMI G'GGCC_C
Cuts at: 2009 2486 2648 2009
Size: 477 162 5362
Fragments arranged by size:
5362 477 162
PstI C_TGCA'G
Cuts at: 830 2581 3353 830
Size: 1751 772 3478
Fragments arranged by size:
3478 1751 772
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6001
PvuII CAG'CTG
Cuts at: 2582 2582
Size: 6001
SacI G_AGCT'C
Cuts at: 2643 2643
Size: 6001
SacII CC_GC'GG
Cuts at: 2016 2016
Size: 6001
SalI G'TCGA_C
Cuts at: 1936 3343 1936
Size: 1407 4594
SanDI GG'GwC_CC
Cuts at: 3043 3043
Size: 6001
SbfI CC_TGCA'GG
Cuts at: 3353 3353
Size: 6001
ScaI AGT'ACT
Cuts at: 2296 4249 5961 2296
Size: 1953 1712 2336
Fragments arranged by size:
2336 1953 1712
SfcI C'TryA_G
Cuts at: 826 1045 2577 3349 4484 5165 5356 826
Size: 219 1532 772 1135 681 191 1471
Fragments arranged by size:
1532 1471 1135 772 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6001
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6001
SmaI CCC'GGG
Cuts at: 1872 3328 1872
Size: 1456 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 4105 5006 5268 820
Size: 197 926 558 1604 901 262 1553
Fragments arranged by size:
1604 1553 926 901 558 262 197
SnaBI TAC'GTA
Cuts at: 493 3792 493
Size: 3299 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6001
SphI G_CATG'C
Cuts at: 1132 2311 3359 1132
Size: 1179 1048 3774
Fragments arranged by size:
3774 1179 1048
SspI AAT'ATT
Cuts at: 5 52 3925 5
Size: 47 3873 2081
Fragments arranged by size:
3873 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6001
StyI C'CwwG_G
Cuts at: 513 1065 2004 2740 3440 513
Size: 552 939 736 700 3074
Fragments arranged by size:
3074 939 736 700 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4028 4045 4198 4383 4828 325
Size: 1255 2448 17 153 185 445 1498
Fragments arranged by size:
2448 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2294 2952 4247
Size: 274 80 33 51 1443 317 658 1295
Cuts at: 4247 5959 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1295 658 317 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2766 2798 2967 2999 5961 5993 2766
Size: 32 169 32 2962 32 2774
Fragments arranged by size:
2962 2774 169 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6001
XbaI T'CTAG_A
Cuts at: 3337 3337
Size: 6001
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6001
XmaI C'CCGG_G
Cuts at: 1870 3326 1870
Size: 1456 4545
XmnI GAAnn'nnTTC
Cuts at: 2814 4130 2814
Size: 1316 4685
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3809 276
Size: 53 83 186 936 2275 2468
Fragments arranged by size:
2468 2275 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI