Restriction map for
pFN21AB6603
(Circular) (Six-base) MAPSORT of: pFN21AB6603.seq Check: 8303 from: 1 to: 5929
pFN21AB6603 5929 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3739 278
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3272 1937
Size: 1335 4594
Acc65I G'GTAC_C
Cuts at: 2143 3250 2143
Size: 1107 4822
AclI AA'CG_TT
Cuts at: 1563 4056 4429 1563
Size: 2493 373 3063
Fragments arranged by size:
3063 2493 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2319 2969 3992 5376 5644 1632
Size: 687 650 1023 1384 268 1917
Fragments arranged by size:
1917 1384 1023 687 650 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5732
AflIII A'CryG_T
Cuts at: 1203 4828 5805 1203
Size: 3625 977 1327
Fragments arranged by size:
3625 1327 977
AgeI A'CCGG_T
Cuts at: 4760 4760
Size: 5929
AhdI GACnn_n'nnGTC
Cuts at: 4658 4658
Size: 5929
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5929
AlwNI CAG_nnn'CTG
Cuts at: 3672 5244 3672
Size: 1572 4357
ApaLI G'TGCA_C
Cuts at: 3986 5142 3986
Size: 1156 4773
ApoI r'AATT_y
Cuts at: 1493 2165 2501 2708 3092 3238 3464 3616 5725
Size: 672 336 207 384 146 226 152 2109
Cuts at: 5725 1493
Size: 1697
Fragments arranged by size:
2109 1697 672 384 336 226 207 152
146
AseI AT'TA_AT
Cuts at: 160 1032 4483 160
Size: 872 3451 1606
Fragments arranged by size:
3451 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
AvaI C'yCGr_G
Cuts at: 1870 1943 3254 1870
Size: 73 1311 4545
Fragments arranged by size:
4545 1311 73
BamHI G'GATC_C
Cuts at: 3259 3626 3259
Size: 367 5562
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2143 3250 4705 618
Size: 325 217 627 356 1107 1455 1842
Fragments arranged by size:
1842 1455 1107 627 356 325 217
BanII G_rGCy'C
Cuts at: 1391 3248 1391
Size: 1857 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2970 928
Size: 971 1071 3887
Fragments arranged by size:
3887 1071 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2552 2586 4120 4154 1903
Size: 34 615 34 1534 34 3678
Fragments arranged by size:
3678 1534 615 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3821 5031 3821
Size: 1210 4719
BclI T'GATC_A
Cuts at: 1548 2728 1548
Size: 1180 4749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2045 4540 136
Size: 107 122 71 631 605 373 2495 1525
Fragments arranged by size:
2495 1525 631 605 373 122 107 71
BglII A'GATC_T
Cuts at: 2336 5924 2336
Size: 3588 2341
BlpI GC'TnA_GC
Cuts at: 3346 3346
Size: 5929
Bme1580I G_kGCm'C
Cuts at: 1790 3990 5146 1790
Size: 2200 1156 2573
Fragments arranged by size:
2573 2200 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5929
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3660 4618 442
Size: 416 53 83 399 24 2243 958 1753
Fragments arranged by size:
2243 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 3240 4589 1239
Size: 824 1177 1349 2579
Fragments arranged by size:
2579 1349 1177 824
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4054 4919 5217 4054
Size: 865 298 4766
Fragments arranged by size:
4766 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2283 2504 3216 4592 882
Size: 617 784 221 712 1376 2219
Fragments arranged by size:
2219 1376 784 712 617 221
BsaAI yAC'GTr
Cuts at: 493 1204 3720 493
Size: 711 2516 2702
Fragments arranged by size:
2702 2516 711
BsaBI GATnn'nnATC
Cuts at: 5923 5923
Size: 5929
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3736 4118 4753 275
Size: 53 83 186 936 2203 382 635 1451
Fragments arranged by size:
2203 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4362 4760 5034 5181 5592 4362
Size: 398 274 147 411 4699
Fragments arranged by size:
4699 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3213 3243 1178
Size: 30 165 30 1810 30 3864
Fragments arranged by size:
3864 1810 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2817 1186
Size: 1631 4298
BseYI C'CCAG_C
Cuts at: 2139 3042 5132 5628 2139
Size: 903 2090 496 2440
Fragments arranged by size:
2440 2090 903 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5356
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3379 4140 5168 1540
Size: 275 186 1378 761 1028 2301
Fragments arranged by size:
2301 1378 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3990 4075 5146 3990
Size: 85 1071 4773
Fragments arranged by size:
4773 1071 85
BsmI GAATG_Cn'
Cuts at: 3440 3533 3440
Size: 93 5836
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3248 3990 4075 5146 1391
Size: 399 1458 742 85 1071 2174
Fragments arranged by size:
2174 1458 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5592 5592
Size: 5929
BspHI T'CATG_A
Cuts at: 3818 5548 3818
Size: 1730 4199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2550 3193 3284 844
Size: 1706 643 91 3489
Fragments arranged by size:
3489 1706 643 91
BsrBI CCG'CTC
Cuts at: 1394 1823 3816 1394
Size: 429 1993 3507
Fragments arranged by size:
3507 1993 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2438 4424 4598 65
Size: 1183 1190 1986 174 1396
Fragments arranged by size:
1986 1396 1190 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4573 4760 1700
Size: 110 2763 187 2869
Fragments arranged by size:
2869 2763 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5929
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5929
BssSI C'ACGA_G
Cuts at: 3989 5001 3989
Size: 1012 4917
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5929
BstBI TT'CG_AA
Cuts at: 4733 4733
Size: 5929
BstEII G'GTnAC_C
Cuts at: 1184 2879 1184
Size: 1695 4234
BstYI r'GATC_y
Cuts at: 1972 2336 3259 3626 4011 4028 5480 5566 5578
Size: 364 923 367 385 17 1452 86 12
Cuts at: 5578 5924 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 923 385 367 364 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2096 513
Size: 552 939 92 4346
Fragments arranged by size:
4346 939 552 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5190
Fragments arranged by size:
5190 683 56
BtsI GCAGTG_nn'
Cuts at: 3445 4239 4259 3445
Size: 794 20 5115
Fragments arranged by size:
5115 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5929
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5894
DraI TTT'AAA
Cuts at: 2830 3013 3233 2830
Size: 183 220 5526
Fragments arranged by size:
5526 220 183
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4936 809
Size: 507 3620 1802
Fragments arranged by size:
3620 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2518 3376 4265
Size: 54 1425 50 275 18 688 858 889
Cuts at: 4265 5894 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 858 688 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3376 1537
Size: 275 1564 4090
Fragments arranged by size:
4090 1564 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3859 1353
Size: 2506 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4516 4890 5036 387
Size: 1064 138 2927 374 146 1280
Fragments arranged by size:
2927 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2319 2969 3240 3992 4589 5376
Size: 393 431 256 650 271 752 597 787
Cuts at: 5376 5644 1239
Size: 268 1524
Fragments arranged by size:
1524 787 752 650 597 431 393 271
268 256
EcoRI G'AATT_C
Cuts at: 3238 3616 3238
Size: 378 5551
FspI TGC'GCA
Cuts at: 4435 4435
Size: 5929
HaeII r_GCGC'y
Cuts at: 1678 5076 1678
Size: 3398 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4570 4602 4644 4676 1373
Size: 32 135 32 2998 32 42 32 2626
Fragments arranged by size:
2998 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3273 3519 669
Size: 988 281 1335 246 3079
Fragments arranged by size:
3079 1335 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5929
HpaI GTT'AAC
Cuts at: 3519 3519
Size: 5929
KpnI G_GTAC'C
Cuts at: 2147 3254 2147
Size: 1107 4822
MfeI C'AATT_G
Cuts at: 3528 3528
Size: 5929
MluI A'CGCG_T
Cuts at: 5805 5805
Size: 5929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5043 5227 1305
Size: 3738 184 2007
Fragments arranged by size:
3738 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2520 10
Size: 54 1425 343 688 3419
Fragments arranged by size:
3419 1425 688 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3887 4246 4405 518
Size: 648 2721 359 159 2042
Fragments arranged by size:
2721 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3345 4022 5170 5415 1606
Size: 1739 677 1148 245 2120
Fragments arranged by size:
2120 1739 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4438
Fragments arranged by size:
4438 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5929
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5929
NotI GC'GGCC_GC
Cuts at: 3376 3376
Size: 5929
NspI r_CATG'y
Cuts at: 1132 1229 3287 4832 1132
Size: 97 2058 1545 2229
Fragments arranged by size:
2229 2058 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5929
PciI A'CATG_T
Cuts at: 4828 4828
Size: 5929
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5929
PmeI GTTT'AAAC
Cuts at: 3233 3233
Size: 5929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3998 4030 5511 5543 3998
Size: 32 1481 32 4384
Fragments arranged by size:
4384 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5929
PsiI TTA'TAA
Cuts at: 3499 3499
Size: 5929
PstI C_TGCA'G
Cuts at: 830 3281 830
Size: 2451 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5929
SalI G'TCGA_C
Cuts at: 1936 3271 1936
Size: 1335 4594
SbfI CC_TGCA'GG
Cuts at: 3281 3281
Size: 5929
ScaI AGT'ACT
Cuts at: 2415 2919 4177 5889 2415
Size: 504 1258 1712 2455
Fragments arranged by size:
2455 1712 1258 504
SexAI A'CCwGG_T
Cuts at: 3225 3225
Size: 5929
SfcI C'TryA_G
Cuts at: 826 1045 2737 3277 4412 5093 5284 826
Size: 219 1692 540 1135 681 191 1471
Fragments arranged by size:
1692 1471 1135 681 540 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5929
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5929
SmaI CCC'GGG
Cuts at: 1872 3256 1872
Size: 1384 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4033 4934 5196 820
Size: 197 926 2090 901 262 1553
Fragments arranged by size:
2090 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3720 493
Size: 3227 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5929
SphI G_CATG'C
Cuts at: 1132 3287 1132
Size: 2155 3774
SspI AAT'ATT
Cuts at: 5 52 2422 3853 5
Size: 47 2370 1431 2081
Fragments arranged by size:
2370 2081 1431 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5929
StyI C'CwwG_G
Cuts at: 513 1065 2004 2229 2841 3368 513
Size: 552 939 225 612 527 3074
Fragments arranged by size:
3074 939 612 552 527 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3956 3973 4126 4311 4756 325
Size: 1255 2376 17 153 185 445 1498
Fragments arranged by size:
2376 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2413 2526 2917
Size: 274 80 33 51 1443 436 113 391
Cuts at: 2917 3067 4175 5887 96
Size: 150 1108 1712 138
Fragments arranged by size:
1712 1443 1108 436 391 274 150 138
113 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5889 5921 5889
Size: 32 5897
Tth111I GACn'n_nGTC
Cuts at: 1316 2795 1316
Size: 1479 4450
XbaI T'CTAG_A
Cuts at: 2680 3265 2680
Size: 585 5344
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2848 3050 2848
Size: 202 5727
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5929
XmaI C'CCGG_G
Cuts at: 1870 3254 1870
Size: 1384 4545
XmnI GAAnn'nnTTC
Cuts at: 4058 4058
Size: 5929
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3737 276
Size: 53 83 186 936 2203 2468
Fragments arranged by size:
2468 2203 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI