Restriction map for
pFN21AB6602
(Circular) (Six-base) MAPSORT of: pFN21AB6602.seq Check: 6040 from: 1 to: 5995
pFN21AB6602 5995 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3338 1937
Size: 1401 4594
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 4122 4495 1563
Size: 2559 373 3063
Fragments arranged by size:
3063 2559 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2149 2972 4058 5442 5710 1632
Size: 517 823 1086 1384 268 1917
Fragments arranged by size:
1917 1384 1086 823 517 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 4894 5871 1203
Size: 3691 977 1327
Fragments arranged by size:
3691 1327 977
AgeI A'CCGG_T
Cuts at: 2043 4826 2043
Size: 2783 3212
AhdI GACnn_n'nnGTC
Cuts at: 2369 2909 4724 2369
Size: 540 1815 3640
Fragments arranged by size:
3640 1815 540
AleI CACnn'nnGTG
Cuts at: 1166 2843 1166
Size: 1677 4318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2474 3224 1184
Size: 1290 750 3955
Fragments arranged by size:
3955 1290 750
AlwNI CAG_nnn'CTG
Cuts at: 2120 2379 3738 5310 2120
Size: 259 1359 1572 2805
Fragments arranged by size:
2805 1572 1359 259
ApaI G_GGCC'C
Cuts at: 2767 2767
Size: 5995
ApaLI G'TGCA_C
Cuts at: 4052 5208 4052
Size: 1156 4839
ApoI r'AATT_y
Cuts at: 1493 3304 3530 3682 5791 1493
Size: 1811 226 152 2109 1697
Fragments arranged by size:
2109 1811 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4549 160
Size: 872 3517 1606
Fragments arranged by size:
3517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 3060 3320 1870
Size: 73 1117 260 4545
Fragments arranged by size:
4545 1117 260 73
BamHI G'GATC_C
Cuts at: 3024 3325 3692 3024
Size: 301 367 5327
Fragments arranged by size:
5327 367 301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2078 3316 4771 618
Size: 325 217 627 278 13 1238 1455 1842
Fragments arranged by size:
1842 1455 1238 627 325 278 217 13
BanII G_rGCy'C
Cuts at: 1391 2057 2129 2419 2559 2767 3314 1391
Size: 666 72 290 140 208 547 4072
Fragments arranged by size:
4072 666 547 290 208 140 72
BbeI G_GCGC'C
Cuts at: 2069 2082 2069
Size: 13 5982
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5024
BbvCI CC'TCA_GC
Cuts at: 2865 2865
Size: 5995
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4186 4220 1903
Size: 34 2249 34 3678
Fragments arranged by size:
3678 2249 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3887 5097 3887
Size: 1210 4785
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5995
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4606 136
Size: 107 122 71 631 605 2934 1525
Fragments arranged by size:
2934 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2421 5990 2421
Size: 3569 2426
BlpI GC'TnA_GC
Cuts at: 3412 3412
Size: 5995
Bme1580I G_kGCm'C
Cuts at: 1790 2767 4056 5212 1790
Size: 977 1289 1156 2573
Fragments arranged by size:
2573 1289 1156 977
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5995
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3726 4684 442
Size: 416 53 83 399 24 2309 958 1753
Fragments arranged by size:
2309 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 2348 2401 2621 3143 4655 1239
Size: 1088 21 53 220 522 1512 2579
Fragments arranged by size:
2579 1512 1088 522 220 53 21
Bpu10I CC'TnA_GC
Cuts at: 2865 2865
Size: 5995
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2606 3059 4120 4985 5283 2606
Size: 453 1061 865 298 3318
Fragments arranged by size:
3318 1061 865 453 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2243 2303 2823 3260 4658 882
Size: 617 744 60 520 437 1398 2219
Fragments arranged by size:
2219 1398 744 617 520 437 60
BsaAI yAC'GTr
Cuts at: 493 1204 3786 493
Size: 711 2582 2702
Fragments arranged by size:
2702 2582 711
BsaBI GATnn'nnATC
Cuts at: 5989 5989
Size: 5995
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 2079 3802 4184
Size: 53 83 186 936 533 13 1723 382
Cuts at: 4184 4819 275
Size: 635 1451
Fragments arranged by size:
1723 1451 936 635 533 382 186 83
53 13
BsaWI w'CCGG_w
Cuts at: 2043 2319 4428 4826 5100 5247 5658 2043
Size: 276 2109 398 274 147 411 2380
Fragments arranged by size:
2380 2109 411 398 276 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2921 2951 1178
Size: 30 165 30 1518 30 4222
Fragments arranged by size:
4222 1518 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2314 2642 2813 2846 1186
Size: 1128 328 171 33 4335
Fragments arranged by size:
4335 1128 328 171 33
BseYI C'CCAG_C
Cuts at: 2069 2120 5198 5694 2069
Size: 51 3078 496 2370
Fragments arranged by size:
3078 2370 496 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3017 1163
Size: 573 1281 4141
Fragments arranged by size:
4141 1281 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2087 2272 3445 4206 5234 1540
Size: 275 186 86 185 1173 761 1028 2301
Fragments arranged by size:
2301 1173 1028 761 275 186 185 86
BsiHKAI G_wGCw'C
Cuts at: 2349 2840 4056 4141 5212 2349
Size: 491 1216 85 1071 3132
Fragments arranged by size:
3132 1216 1071 491 85
BsmI GAATG_Cn'
Cuts at: 3506 3599 3506
Size: 93 5902
BsmBI CGTCTCn'nnnn_
Cuts at: 2876 2896 2876
Size: 20 5975
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2129 2349 2419 2559 2767 2840
Size: 399 267 72 220 70 140 208 73
Cuts at: 2840 3314 4056 4141 5212 1391
Size: 474 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 474 399 267 220 208
140 85 73 72 70
BspEI T'CCGG_A
Cuts at: 5658 5658
Size: 5995
BspHI T'CATG_A
Cuts at: 2640 3884 5614 2640
Size: 1244 1730 3021
Fragments arranged by size:
3021 1730 1244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3350 844
Size: 2506 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2154 3882 1394
Size: 429 331 1728 3507
Fragments arranged by size:
3507 1728 429 331
BsrDI GCAATG_nn'
Cuts at: 65 1248 4490 4664 65
Size: 1183 3242 174 1396
Fragments arranged by size:
3242 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2043 2086 4639 4826 1700
Size: 110 233 43 2553 187 2869
Fragments arranged by size:
2869 2553 233 187 110 43
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5995
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5995
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5995
BstBI TT'CG_AA
Cuts at: 4799 4799
Size: 5995
BstEII G'GTnAC_C
Cuts at: 1184 2848 1184
Size: 1664 4331
BstXI CCAn_nnnn'nTGG
Cuts at: 2749 2749
Size: 5995
BstYI r'GATC_y
Cuts at: 1972 2139 2421 3024 3325 3692 4077 4094 5546
Size: 167 282 603 301 367 385 17 1452
Cuts at: 5546 5632 5644 5990 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 603 385 367 346 301 282
167 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2411 2975 2411
Size: 564 5431
BtgI C'CryG_G
Cuts at: 513 1065 2551 2706 513
Size: 552 1486 155 3802
Fragments arranged by size:
3802 1486 552 155
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5256
Fragments arranged by size:
5256 683 56
BtsI GCAGTG_nn'
Cuts at: 3511 4305 4325 3511
Size: 794 20 5181
Fragments arranged by size:
5181 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2418 2453 529
Size: 35 1854 35 4071
Fragments arranged by size:
4071 1854 35 35
DraI TTT'AAA
Cuts at: 3299 3299
Size: 5995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2498 5002 809
Size: 507 1182 2504 1802
Fragments arranged by size:
2504 1802 1182 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2084 2100 2193
Size: 54 1425 50 275 18 254 16 93
Cuts at: 2193 2520 3442 4331 5960 8
Size: 327 922 889 1629 43
Fragments arranged by size:
1629 1425 922 889 327 275 254 93
54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2084 3442 1537
Size: 275 272 1358 4090
Fragments arranged by size:
4090 1358 275 272
EarI CTCTTCn'nnn_
Cuts at: 1353 2642 2652 3925 1353
Size: 1289 10 1273 3423
Fragments arranged by size:
3423 1289 1273 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4582 4956 5102 387
Size: 1064 138 2993 374 146 1280
Fragments arranged by size:
2993 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2149 2327 2348 2401 2621 2972 3143
Size: 393 517 178 21 53 220 351 171
Cuts at: 3143 4058 4655 5442 5710 1239
Size: 915 597 787 268 1524
Fragments arranged by size:
1524 915 787 597 517 393 351 268
220 178 171 53 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2978 2978
Size: 5995
EcoO109I rG'GnC_Cy
Cuts at: 2188 2363 2407 2734 3097 2188
Size: 175 44 327 363 5086
Fragments arranged by size:
5086 363 327 175 44
EcoRI G'AATT_C
Cuts at: 3304 3682 3304
Size: 378 5617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2977 2977
Size: 5995
FspI TGC'GCA
Cuts at: 4501 4501
Size: 5995
HaeII r_GCGC'y
Cuts at: 1678 2069 2082 2121 3079 5142 1678
Size: 391 13 39 958 2063 2531
Fragments arranged by size:
2531 2063 958 391 39 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2355 2387 2602 2634 2895
Size: 32 135 32 783 32 215 32 261
Cuts at: 2895 2921 2927 2953 4636 4668 4710 4742 1373
Size: 26 6 26 1683 32 42 32 2626
Fragments arranged by size:
2626 1683 783 261 215 135 42 32
32 32 32 32 32 26 26 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 3339 3585 669
Size: 988 281 765 636 246 3079
Fragments arranged by size:
3079 988 765 636 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5995
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KasI G'GCGC_C
Cuts at: 2065 2078 2065
Size: 13 5982
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3594 3594
Size: 5995
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2851 5109 5293 1305
Size: 1546 2258 184 2007
Fragments arranged by size:
2258 2007 1546 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2195 2522 10
Size: 54 1425 343 363 327 3483
Fragments arranged by size:
3483 1425 363 343 327 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2843 3953 4312 4471 518
Size: 648 1677 1110 359 159 2042
Fragments arranged by size:
2042 1677 1110 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2599 3411 4088 5236 5481 1606
Size: 993 812 677 1148 245 2120
Fragments arranged by size:
2120 1148 993 812 677 245
NarI GG'CG_CC
Cuts at: 2066 2079 2066
Size: 13 5982
NcoI C'CATG_G
Cuts at: 513 1065 2551 513
Size: 552 1486 3957
Fragments arranged by size:
3957 1486 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5995
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 3296 3353 4898 1132
Size: 97 2067 57 1545 2229
Fragments arranged by size:
2229 2067 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 2050 1348
Size: 702 5293
PciI A'CATG_T
Cuts at: 4894 4894
Size: 5995
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2536 2920 1108
Size: 1428 384 4183
Fragments arranged by size:
4183 1428 384
PfoI T'CCnGG_A
Cuts at: 2135 2340 2135
Size: 205 5790
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 5577 5609 4064
Size: 32 1481 32 4450
Fragments arranged by size:
4450 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2363 2407 2734 2363
Size: 44 327 5624
Fragments arranged by size:
5624 327 44
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5995
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PspOMI G'GGCC_C
Cuts at: 2763 2763
Size: 5995
PstI C_TGCA'G
Cuts at: 830 2463 2598 3347 830
Size: 1633 135 749 3478
Fragments arranged by size:
3478 1633 749 135
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
PvuII CAG'CTG
Cuts at: 2599 2599
Size: 5995
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SanDI GG'GwC_CC
Cuts at: 2363 2363
Size: 5995
SapI GCTCTTCn'nnn_
Cuts at: 2642 2642
Size: 5995
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 2813 4243 5955 2813
Size: 1430 1712 2853
Fragments arranged by size:
2853 1712 1430
SfcI C'TryA_G
Cuts at: 826 1045 2459 2594 3002 3343 4478 5159 5350
Size: 219 1414 135 408 341 1135 681 191
Cuts at: 5350 826
Size: 1471
Fragments arranged by size:
1471 1414 1135 681 408 341 219 191
135
SfoI GGC'GCC
Cuts at: 2067 2080 2067
Size: 13 5982
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 3062 3322 1872
Size: 1190 260 4545
Fragments arranged by size:
4545 1190 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2621 3038 4099 5000 5262 820
Size: 197 926 678 417 1061 901 262 1553
Fragments arranged by size:
1553 1061 926 901 678 417 262 197
SnaBI TAC'GTA
Cuts at: 493 3786 493
Size: 3293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 3353 1132
Size: 2221 3774
SspI AAT'ATT
Cuts at: 5 52 3919 5
Size: 47 3867 2081
Fragments arranged by size:
3867 2081 47
StuI AGG'CCT
Cuts at: 1517 2673 1517
Size: 1156 4839
StyI C'CwwG_G
Cuts at: 513 1065 2551 2919 3189 3434 513
Size: 552 1486 368 270 245 3074
Fragments arranged by size:
3074 1486 552 368 270 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2327 4022 4039 4192 4377 4822 325
Size: 1255 747 1695 17 153 185 445 1498
Fragments arranged by size:
1695 1498 1255 747 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2811 4241 5953
Size: 274 80 33 51 1443 834 1430 1712
Cuts at: 5953 96
Size: 138
Fragments arranged by size:
1712 1443 1430 834 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5955 5987 5955
Size: 32 5963
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 3331 3331
Size: 5995
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 3060 3320 1870
Size: 1190 260 4545
Fragments arranged by size:
4545 1190 260
XmnI GAAnn'nnTTC
Cuts at: 4124 4124
Size: 5995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SexAI SfiI SrfI SwaI XcmI