Restriction map for
pFN21AB6594
(Circular) (Six-base) MAPSORT of: pFN21AB6594.seq Check: 2126 from: 1 to: 5923
pFN21AB6594 5923 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3157 3157
Size: 5923
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3185 3733 278
Size: 53 83 186 936 1649 548 2468
Fragments arranged by size:
2468 1649 936 548 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2328 3266 1937
Size: 391 938 4594
Fragments arranged by size:
4594 938 391
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2393 3986 5370 5638 1632
Size: 761 1593 1384 268 1917
Fragments arranged by size:
1917 1593 1384 761 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 2955 1166
Size: 1789 4134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2973 1184
Size: 1789 4134
AlwNI CAG_nnn'CTG
Cuts at: 2068 2086 2236 3666 5238 2068
Size: 18 150 1430 1572 2753
Fragments arranged by size:
2753 1572 1430 150 18
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 2615 3232 3458 3610 5719 1493
Size: 1122 617 226 152 2109 1697
Fragments arranged by size:
2109 1697 1122 617 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 3248 1870
Size: 73 1305 4545
Fragments arranged by size:
4545 1305 73
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2957 3244 4699 618
Size: 325 217 627 1170 287 1455 1842
Fragments arranged by size:
1842 1455 1170 627 325 287 217
BanII G_rGCy'C
Cuts at: 1391 3242 1391
Size: 1851 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2734 928
Size: 971 835 4117
Fragments arranged by size:
4117 971 835
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4114 4148 1903
Size: 34 2177 34 3678
Fragments arranged by size:
3678 2177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2511 3815 5025 2511
Size: 1304 1210 3409
Fragments arranged by size:
3409 1304 1210
BclI T'GATC_A
Cuts at: 1548 2850 3012 1548
Size: 1302 162 4459
Fragments arranged by size:
4459 1302 162
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2375 4534 136
Size: 107 122 71 631 605 703 2159 1525
Fragments arranged by size:
2159 1525 703 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 2180 3340 2180
Size: 1160 4763
Bme1580I G_kGCm'C
Cuts at: 1790 3984 5140 1790
Size: 2194 1156 2573
Fragments arranged by size:
2573 2194 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2056 2109 3654
Size: 416 53 83 399 24 639 53 1545
Cuts at: 3654 4612 442
Size: 958 1753
Fragments arranged by size:
1753 1545 958 639 416 399 83 53
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4583 1239
Size: 3344 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2525 3714 493
Size: 711 1321 1189 2702
Fragments arranged by size:
2702 1321 1189 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3182 3730 4112 4747
Size: 53 83 186 936 1649 548 382 635
Cuts at: 4747 275
Size: 1451
Fragments arranged by size:
1649 1451 936 635 548 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3059 4356 4754 5028 5175 5586 3059
Size: 1297 398 274 147 411 3396
Fragments arranged by size:
3396 1297 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5698
Fragments arranged by size:
5698 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3161 1186
Size: 1975 3948
BseYI C'CCAG_C
Cuts at: 2092 2120 3027 5126 5622 2092
Size: 28 907 2099 496 2393
Fragments arranged by size:
2393 2099 907 496 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5350
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2985 3984 4069 5140 2985
Size: 999 85 1071 3768
Fragments arranged by size:
3768 1071 999 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
BsmBI CGTCTCn'nnnn_
Cuts at: 3173 3173
Size: 5923
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2985 3242 3984 4069 5140 1391
Size: 399 1195 257 742 85 1071 2174
Fragments arranged by size:
2174 1195 1071 742 399 257 85
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3157 3278 844
Size: 2313 121 3489
Fragments arranged by size:
3489 2313 121
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4567 4754 1700
Size: 110 2757 187 2869
Fragments arranged by size:
2869 2757 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2419 3253 3620 4005 4022 5474 5560 5572
Size: 447 834 367 385 17 1452 86 12
Cuts at: 5572 5918 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 834 447 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2103 2457 2732 513
Size: 552 939 99 354 275 3704
Fragments arranged by size:
3704 939 552 354 275 99
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 3439 4233 4253 3439
Size: 794 20 5109
Fragments arranged by size:
5109 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3370 4259 5888
Size: 54 1425 50 275 18 1540 889 1629
Cuts at: 5888 8
Size: 43
Fragments arranged by size:
1629 1540 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3853 1353
Size: 2500 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2393 3986 4583 5370 5638 1239
Size: 393 761 1593 597 787 268 1524
Fragments arranged by size:
1593 1524 787 761 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2233 2233
Size: 5923
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 5070 1678
Size: 3392 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 2914 2946 3004
Size: 32 135 32 448 32 862 32 58
Cuts at: 3004 3025 3036 3057 4564 4596 4638 4670 1373
Size: 21 11 21 1507 32 42 32 2626
Fragments arranged by size:
2626 1507 862 448 135 58 42 32
32 32 32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2252 2329 2967 3161 3267 3513
Size: 988 281 314 77 638 194 106 246
Cuts at: 3513 669
Size: 3079
Fragments arranged by size:
3079 988 638 314 281 246 194 106
77
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 2967 3513 2967
Size: 546 5377
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4101
Fragments arranged by size:
4101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2607 2955 3881 4240 4399 518
Size: 648 1441 348 926 359 159 2042
Fragments arranged by size:
2042 1441 926 648 359 348 159
MspA1I CmG'CkG
Cuts at: 1606 2795 3134 3339 4016 5164 5409 1606
Size: 1189 339 205 677 1148 245 2120
Fragments arranged by size:
2120 1189 1148 677 339 245 205
NcoI C'CATG_G
Cuts at: 513 1065 2004 2103 2457 2732 513
Size: 552 939 99 354 275 3704
Fragments arranged by size:
3704 939 552 354 275 99
NdeI CA'TA_TG
Cuts at: 387 2257 3120 387
Size: 1870 863 3190
Fragments arranged by size:
3190 1870 863
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 3073 3281 4826 1132
Size: 97 1844 208 1545 2229
Fragments arranged by size:
2229 1844 1545 208 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2104 2236 1108
Size: 996 132 4795
Fragments arranged by size:
4795 996 132
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2233 2233
Size: 5923
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PstI C_TGCA'G
Cuts at: 830 2347 3275 830
Size: 1517 928 3478
Fragments arranged by size:
3478 1517 928
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 2795 2795
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 2327 3265 1936
Size: 391 938 4594
Fragments arranged by size:
4594 938 391
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SexAI A'CCwGG_T
Cuts at: 2235 2901 2235
Size: 666 5257
SfcI C'TryA_G
Cuts at: 826 1045 2343 2790 2945 2996 3271 4406 5087
Size: 219 1298 447 155 51 275 1135 681
Cuts at: 5087 5278 826
Size: 191 1471
Fragments arranged by size:
1471 1298 1135 681 447 275 219 191
155 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4027 4928 5190 820
Size: 197 926 2084 901 262 1553
Fragments arranged by size:
2084 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2004 2103 2457 2732 3362 513
Size: 552 939 99 354 275 630 3074
Fragments arranged by size:
3074 939 630 552 354 275 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3950 3967 4120 4305 4750 325
Size: 1255 2370 17 153 185 445 1498
Fragments arranged by size:
2370 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4169 5881 96
Size: 274 80 33 51 1443 2192 1712 138
Fragments arranged by size:
2192 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2509 2541 5883 5915 2509
Size: 32 3342 32 2517
Fragments arranged by size:
3342 2517 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2083 2083
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3183 3731 276
Size: 53 83 186 936 1649 548 2468
Fragments arranged by size:
2468 1649 936 548 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI