Restriction map for
pFN21AB6593
(Circular) (Six-base) MAPSORT of: pFN21AB6593.seq Check: 4537 from: 1 to: 5836
pFN21AB6593 5836 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2315 2784 2807 2993 3899 5283 5551 1632
Size: 683 469 23 186 906 1384 268 1917
Fragments arranged by size:
1917 1384 906 683 469 268 186 23
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 2478 4735 5712 1203
Size: 1275 2257 977 1327
Fragments arranged by size:
2257 1327 1275 977
AgeI A'CCGG_T
Cuts at: 2501 4667 2501
Size: 2166 3670
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 2024 1166
Size: 858 4978
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 5804
AlwNI CAG_nnn'CTG
Cuts at: 2207 2729 3579 5151 2207
Size: 522 850 1572 2892
Fragments arranged by size:
2892 1572 850 522
ApaI G_GGCC'C
Cuts at: 2535 2535
Size: 5836
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 2618 3145 3371 3523 5632 1493
Size: 1125 527 226 152 2109 1697
Fragments arranged by size:
2109 1697 1125 527 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2999 3161 1870
Size: 73 1056 162 4545
Fragments arranged by size:
4545 1056 162 73
BamHI G'GATC_C
Cuts at: 3040 3166 3533 3040
Size: 126 367 5343
Fragments arranged by size:
5343 367 126
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2585 2603 3157 4612 618
Size: 325 217 627 798 18 554 1455 1842
Fragments arranged by size:
1842 1455 798 627 554 325 217 18
BanII G_rGCy'C
Cuts at: 1391 2535 3077 3155 1391
Size: 1144 542 78 4072
Fragments arranged by size:
4072 1144 542 78
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2772 3132 928
Size: 971 873 360 3632
Fragments arranged by size:
3632 971 873 360
BbvCI CC'TCA_GC
Cuts at: 2736 2736
Size: 5836
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4027 4061 1903
Size: 34 2090 34 3678
Fragments arranged by size:
3678 2090 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2225 3728 4938 2225
Size: 1503 1210 3123
Fragments arranged by size:
3123 1503 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2288 2530 2714
Size: 107 122 71 631 605 616 242 184
Cuts at: 2714 3089 4447 136
Size: 375 1358 1525
Fragments arranged by size:
1525 1358 631 616 605 375 242 184
122 107 71
BglII A'GATC_T
Cuts at: 5831 5831
Size: 5836
BlpI GC'TnA_GC
Cuts at: 2938 3253 2938
Size: 315 5521
Bme1580I G_kGCm'C
Cuts at: 1790 2535 3897 5053 1790
Size: 745 1362 1156 2573
Fragments arranged by size:
2573 1362 1156 745
BmgBI CAC'GTC
Cuts at: 1318 2986 1318
Size: 1668 4168
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2345 2529 2628
Size: 416 53 83 399 24 928 184 99
Cuts at: 2628 2766 2832 3567 4525 442
Size: 138 66 735 958 1753
Fragments arranged by size:
1753 958 928 735 416 399 184 138
99 83 66 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4496 1239
Size: 3257 2579
Bpu10I CC'TnA_GC
Cuts at: 2646 2736 2837 2646
Size: 90 101 5645
Fragments arranged by size:
5645 101 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 3961 4826 5124 2381
Size: 1580 865 298 3093
Fragments arranged by size:
3093 1580 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4499 882
Size: 617 3000 2219
Fragments arranged by size:
3000 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3627 493
Size: 711 2423 2702
Fragments arranged by size:
2702 2423 711
BsaBI GATnn'nnATC
Cuts at: 2327 2795 5830 2327
Size: 468 3035 2333
Fragments arranged by size:
3035 2333 468
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3643 4025 4660 275
Size: 53 83 186 936 2110 382 635 1451
Fragments arranged by size:
2110 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2501 4269 4667 4941 5088 5499 2501
Size: 1768 398 274 147 411 2838
Fragments arranged by size:
2838 1768 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2509 2539 1178
Size: 30 165 30 1106 30 4475
Fragments arranged by size:
4475 1106 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 2714 2717 2984 1186
Size: 1270 258 3 267 4038
Fragments arranged by size:
4038 1270 267 258 3
BseYI C'CCAG_C
Cuts at: 2088 2593 5039 5535 2088
Size: 505 2446 496 2389
Fragments arranged by size:
2446 2389 505 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2248 1163
Size: 573 512 4751
Fragments arranged by size:
4751 573 512
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3286 4047 5075 1540
Size: 275 186 1285 761 1028 2301
Fragments arranged by size:
2301 1285 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3897 3982 5053 3897
Size: 85 1071 4680
Fragments arranged by size:
4680 1071 85
BsmI GAATG_Cn'
Cuts at: 3347 3440 3347
Size: 93 5743
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2535 3077 3155 3897 3982 5053 1391
Size: 399 745 542 78 742 85 1071 2174
Fragments arranged by size:
2174 1071 745 742 542 399 85 78
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 3725 5455 3725
Size: 1730 4106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3191 844
Size: 2347 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2331 4331 4505 65
Size: 1183 1083 2000 174 1396
Fragments arranged by size:
2000 1396 1183 1083 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2501 4480 4667 1700
Size: 110 691 1979 187 2869
Fragments arranged by size:
2869 1979 691 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 3896 4908 3896
Size: 1012 4824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2729 794
Size: 1935 3901
BstBI TT'CG_AA
Cuts at: 3061 4640 3061
Size: 1579 4257
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5836
BstYI r'GATC_y
Cuts at: 1972 2371 2629 3040 3166 3533 3918 3935 5387
Size: 399 258 411 126 367 385 17 1452
Cuts at: 5387 5473 5485 5831 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 411 399 385 367 346 258
126 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4345
Fragments arranged by size:
4345 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5097
Fragments arranged by size:
5097 683 56
BtsI GCAGTG_nn'
Cuts at: 3352 4146 4166 3352
Size: 794 20 5022
Fragments arranged by size:
5022 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4843 809
Size: 507 3527 1802
Fragments arranged by size:
3527 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2209 2815 2905
Size: 54 1425 50 275 18 379 606 90
Cuts at: 2905 3283 4172 5801 8
Size: 378 889 1629 43
Fragments arranged by size:
1629 1425 889 606 379 378 275 90
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3283 1537
Size: 275 1471 4090
Fragments arranged by size:
4090 1471 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2433 2673 2850 3766 1353
Size: 1080 240 177 916 3423
Fragments arranged by size:
3423 1080 916 240 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2927 4423 4797 4943 387
Size: 1064 138 1338 1496 374 146 1280
Fragments arranged by size:
1496 1338 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2784 2807 2993 3899 4496 5283
Size: 393 683 469 23 186 906 597 787
Cuts at: 5283 5551 1239
Size: 268 1524
Fragments arranged by size:
1524 906 787 683 597 469 393 268
186 23
EcoNI CCTnn'n_nnAGG
Cuts at: 2399 2399
Size: 5836
EcoO109I rG'GnC_Cy
Cuts at: 2995 2995
Size: 5836
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 5836
FspI TGC'GCA
Cuts at: 2327 4342 2327
Size: 2015 3821
FspAI rTGC'GCAy
Cuts at: 2327 2327
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 4983 1678
Size: 3305 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2509 2541 2921 2953 4477
Size: 32 135 32 937 32 380 32 1524
Cuts at: 4477 4509 4551 4583 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1524 937 380 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2104 2419 3180 3426 669
Size: 988 281 166 315 761 246 3079
Fragments arranged by size:
3079 988 761 315 281 246 166
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1992 2263 4950 5134 1305
Size: 687 271 2687 184 2007
Fragments arranged by size:
2687 2007 687 271 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2907 10
Size: 54 1425 343 1075 2939
Fragments arranged by size:
2939 1425 1075 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2024 2276 2879 3794 4153 4312 518
Size: 648 858 252 603 915 359 159 2042
Fragments arranged by size:
2042 915 858 648 603 359 252 159
MspA1I CmG'CkG
Cuts at: 1606 2686 2692 2720 3252 3929 5077 5322 1606
Size: 1080 6 28 532 677 1148 245 2120
Fragments arranged by size:
2120 1148 1080 677 532 245 28 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4345
Fragments arranged by size:
4345 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 3194 4739 1132
Size: 97 1965 1545 2229
Fragments arranged by size:
2229 1965 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5836
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2207 1108
Size: 1099 4737
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2523 3905 3937 5418 5450 2491
Size: 32 1382 32 1481 32 2877
Fragments arranged by size:
2877 1481 1382 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2995 2995
Size: 5836
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PspOMI G'GGCC_C
Cuts at: 2531 2531
Size: 5836
PstI C_TGCA'G
Cuts at: 830 2085 2725 2892 3188 830
Size: 1255 640 167 296 3478
Fragments arranged by size:
3478 1255 640 296 167
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2720 2720
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SapI GCTCTTCn'nnn_
Cuts at: 2673 2850 2673
Size: 177 5659
SbfI CC_TGCA'GG
Cuts at: 2892 3188 2892
Size: 296 5540
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SfcI C'TryA_G
Cuts at: 826 1045 2081 2721 2888 3184 4319 5000 5191
Size: 219 1036 640 167 296 1135 681 191
Cuts at: 5191 826
Size: 1471
Fragments arranged by size:
1471 1135 1036 681 640 296 219 191
167
SfiI GGCCn_nnn'nGGCC
Cuts at: 3089 3089
Size: 5836
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2396 3940 4841 5103 820
Size: 197 926 453 1544 901 262 1553
Fragments arranged by size:
1553 1544 926 901 453 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 3194 1132
Size: 2062 3774
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 2004 2244 2655 2964 3096 3275 513
Size: 552 939 240 411 309 132 179 3074
Fragments arranged by size:
3074 939 552 411 309 240 179 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3863 3880 4033 4218 4663 325
Size: 1255 2283 17 153 185 445 1498
Fragments arranged by size:
2283 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4082 5794 96
Size: 274 80 33 51 1443 2105 1712 138
Fragments arranged by size:
2105 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2337 2369 2812 2844 5796 5828 2337
Size: 32 443 32 2952 32 2345
Fragments arranged by size:
2952 2345 443 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5836
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2810 2941 2810
Size: 131 5705
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 2503 3057 3965 2503
Size: 554 908 4374
Fragments arranged by size:
4374 908 554
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfoI SrfI SwaI