Restriction map for
pFN21AB6588
(Circular) (Six-base) MAPSORT of: pFN21AB6588.seq Check: 4615 from: 1 to: 5923
pFN21AB6588 5923 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2295 2397 3266 1937
Size: 358 102 869 4594
Fragments arranged by size:
4594 869 358 102
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2298 3096 3986 5370 5638 1632
Size: 666 798 890 1384 268 1917
Fragments arranged by size:
1917 1384 890 798 666 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 3198 4822 5799 1203
Size: 1995 1624 977 1327
Fragments arranged by size:
1995 1624 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3114 3146 3114
Size: 32 5891
AlwNI CAG_nnn'CTG
Cuts at: 2200 2585 3074 3101 3666 5238 2200
Size: 385 489 27 565 1572 2885
Fragments arranged by size:
2885 1572 565 489 385 27
ApaLI G'TGCA_C
Cuts at: 3091 3980 5136 3091
Size: 889 1156 3878
Fragments arranged by size:
3878 1156 889
ApoI r'AATT_y
Cuts at: 1493 2285 2669 2951 3232 3458 3610 5719 1493
Size: 792 384 282 281 226 152 2109 1697
Fragments arranged by size:
2109 1697 792 384 282 281 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2604 3021 3248 1870
Size: 73 661 417 227 4545
Fragments arranged by size:
4545 661 417 227 73
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3244 4699 618
Size: 325 217 627 1457 1455 1842
Fragments arranged by size:
1842 1457 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2216 2526 3054 3242 1391
Size: 825 310 528 188 4072
Fragments arranged by size:
4072 825 528 310 188
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2597 928
Size: 971 698 4254
Fragments arranged by size:
4254 971 698
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2144 2178 4114 4148 1903
Size: 34 207 34 1936 34 3678
Fragments arranged by size:
3678 1936 207 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4534 136
Size: 107 122 71 631 605 2862 1525
Fragments arranged by size:
2862 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 2547 3048 3340 2547
Size: 501 292 5130
Fragments arranged by size:
5130 501 292
Bme1580I G_kGCm'C
Cuts at: 1790 3095 3984 5140 1790
Size: 1305 889 1156 2573
Fragments arranged by size:
2573 1305 1156 889
BmgBI CAC'GTC
Cuts at: 1318 2299 1318
Size: 981 4942
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2546 2595 3654
Size: 416 53 83 399 24 1129 49 1059
Cuts at: 3654 4612 442
Size: 958 1753
Fragments arranged by size:
1753 1129 1059 958 416 399 83 53
49 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2230 2230
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2057 2081 2562 2801 2814 2846 4583 1239
Size: 818 24 481 239 13 32 1737 2579
Fragments arranged by size:
2579 1737 818 481 239 32 24 13
Bpu10I CC'TnA_GC
Cuts at: 2520 2520
Size: 5923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 2900 5917 2900
Size: 3017 2906
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3730 4112 4747 275
Size: 53 83 186 936 2197 382 635 1451
Fragments arranged by size:
2197 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2130 4356 4754 5028 5175 5586 2130
Size: 2226 398 274 147 411 2467
Fragments arranged by size:
2467 2226 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5698
Fragments arranged by size:
5698 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2201 2761 2984 1186
Size: 868 147 560 223 4125
Fragments arranged by size:
4125 868 560 223 147
BseYI C'CCAG_C
Cuts at: 5126 5622 5126
Size: 496 5427
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2357 2831 1163
Size: 573 621 474 4255
Fragments arranged by size:
4255 621 573 474
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2526 3054 3095 3984 4069 5140 2526
Size: 528 41 889 85 1071 3309
Fragments arranged by size:
3309 1071 889 528 85 41
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2526 3054 3095 3242 3984 4069
Size: 399 426 310 528 41 147 742 85
Cuts at: 4069 5140 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 528 426 399 310 147
85 41
BspEI T'CCGG_A
Cuts at: 2130 5586 2130
Size: 3456 2467
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3278 844
Size: 2434 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2541 4418 4592 65
Size: 1183 1293 1877 174 1396
Fragments arranged by size:
1877 1396 1293 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4567 4754 1700
Size: 110 2757 187 2869
Fragments arranged by size:
2869 2757 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 2529 4727 2529
Size: 2198 3725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 2379 3253 3620 4005 4022 5474 5560 5572
Size: 407 874 367 385 17 1452 86 12
Cuts at: 5572 5918 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 874 407 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2241 513
Size: 552 939 237 4195
Fragments arranged by size:
4195 939 552 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 2915 3439 4233 4253 2915
Size: 524 794 20 4585
Fragments arranged by size:
4585 794 524 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3370 4259 5888
Size: 54 1425 50 275 18 1540 889 1629
Cuts at: 5888 8
Size: 43
Fragments arranged by size:
1629 1540 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2531 3853 1353
Size: 1178 1322 3423
Fragments arranged by size:
3423 1322 1178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2054 4510 4884 5030 387
Size: 1064 138 465 2456 374 146 1280
Fragments arranged by size:
2456 1280 1064 465 374 146 138
EcoICRI GAG'CTC
Cuts at: 2524 3052 2524
Size: 528 5395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2057 2081 2298 2562 2801 2814 2846
Size: 393 425 24 217 264 239 13 32
Cuts at: 2846 3096 3986 4583 5370 5638 1239
Size: 250 890 597 787 268 1524
Fragments arranged by size:
1524 890 787 597 425 393 268 264
250 239 217 32 24 13
EcoRI G'AATT_C
Cuts at: 2951 3232 3610 2951
Size: 281 378 5264
Fragments arranged by size:
5264 378 281
EcoRV GAT'ATC
Cuts at: 2902 2902
Size: 5923
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 2012 5070 1678
Size: 334 3058 2531
Fragments arranged by size:
3058 2531 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2986 3018 3120 3152 4564
Size: 32 135 32 1414 32 102 32 1412
Cuts at: 4564 4596 4638 4670 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1414 1412 135 102 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 2335 3522 2335
Size: 1187 4736
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4101
Fragments arranged by size:
4101 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2744 3881 4240 4399 518
Size: 648 1578 1137 359 159 2042
Fragments arranged by size:
2042 1578 1137 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2058 2101 2197 3339 4016 5164 5409 1606
Size: 452 43 96 1142 677 1148 245 2120
Fragments arranged by size:
2120 1148 1142 677 452 245 96 43
NcoI C'CATG_G
Cuts at: 513 1065 2004 2241 513
Size: 552 939 237 4195
Fragments arranged by size:
4195 939 552 237
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 2729 3109 3202 3281 4826 1132
Size: 97 1500 380 93 79 1545 2229
Fragments arranged by size:
2229 1545 1500 380 97 93 79
PasI CC'CwG_GG
Cuts at: 1348 2927 1348
Size: 1579 4344
PciI A'CATG_T
Cuts at: 3198 4822 3198
Size: 1624 4299
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2585 1108
Size: 1477 4446
PfoI T'CCnGG_A
Cuts at: 2904 2904
Size: 5923
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PstI C_TGCA'G
Cuts at: 830 2142 2205 3275 830
Size: 1312 63 1070 3478
Fragments arranged by size:
3478 1312 1070 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 2101 2197 2101
Size: 96 5827
SacI G_AGCT'C
Cuts at: 2526 3054 2526
Size: 528 5395
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SapI GCTCTTCn'nnn_
Cuts at: 2531 2531
Size: 5923
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 2138 2192 2201 3271 4406 5087 5278
Size: 219 1093 54 9 1070 1135 681 191
Cuts at: 5278 826
Size: 1471
Fragments arranged by size:
1471 1135 1093 1070 681 219 191 54
9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 2606 3023 3250 1872
Size: 734 417 227 4545
Fragments arranged by size:
4545 734 417 227
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4027 4928 5190 820
Size: 197 926 2084 901 262 1553
Fragments arranged by size:
2084 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SrfI GCCC'GGGC
Cuts at: 2606 2606
Size: 5923
SspI AAT'ATT
Cuts at: 5 52 2072 3847 5
Size: 47 2020 1775 2081
Fragments arranged by size:
2081 2020 1775 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2004 2241 2553 3168 3362 513
Size: 552 939 237 312 615 194 3074
Fragments arranged by size:
3074 939 615 552 312 237 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2347 3950 3967 4120 4305 4750 325
Size: 1255 767 1603 17 153 185 445 1498
Fragments arranged by size:
1603 1498 1255 767 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4169 5881 96
Size: 274 80 33 51 1443 2192 1712 138
Fragments arranged by size:
2192 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5883 5915 5883
Size: 32 5891
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2341 2341
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 2604 3021 3248 1870
Size: 734 417 227 4545
Fragments arranged by size:
4545 734 417 227
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SwaI