Restriction map for
pFN21AB6579
(Circular) (Six-base) MAPSORT of: pFN21AB6579.seq Check: 4716 from: 1 to: 5836
pFN21AB6579 5836 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3646 278
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3179 1937
Size: 1242 4594
Acc65I G'GTAC_C
Cuts at: 3157 3157
Size: 5836
AclI AA'CG_TT
Cuts at: 1563 3963 4336 1563
Size: 2400 373 3063
Fragments arranged by size:
3063 2400 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2231 3899 5283 5551 1632
Size: 599 1668 1384 268 1917
Fragments arranged by size:
1917 1668 1384 599 268
AfeI AGC'GCT
Cuts at: 2771 2771
Size: 5836
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5639
AflIII A'CryG_T
Cuts at: 1203 4735 5712 1203
Size: 3532 977 1327
Fragments arranged by size:
3532 1327 977
AgeI A'CCGG_T
Cuts at: 4667 4667
Size: 5836
AhdI GACnn_n'nnGTC
Cuts at: 4565 4565
Size: 5836
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5836
AlwNI CAG_nnn'CTG
Cuts at: 3579 5151 3579
Size: 1572 4264
ApaLI G'TGCA_C
Cuts at: 3893 5049 3893
Size: 1156 4680
ApoI r'AATT_y
Cuts at: 1493 3145 3371 3523 5632 1493
Size: 1652 226 152 2109 1697
Fragments arranged by size:
2109 1697 1652 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4390 160
Size: 872 3358 1606
Fragments arranged by size:
3358 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
AvaI C'yCGr_G
Cuts at: 1870 1943 2303 3161 1870
Size: 73 360 858 4545
Fragments arranged by size:
4545 858 360 73
AvrII C'CTAG_G
Cuts at: 2090 2090
Size: 5836
BamHI G'GATC_C
Cuts at: 3166 3533 3166
Size: 367 5469
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2268 2915 2971 3157 4612
Size: 325 217 627 481 647 56 186 1455
Cuts at: 4612 618
Size: 1842
Fragments arranged by size:
1842 1455 647 627 481 325 217 186
56
BanII G_rGCy'C
Cuts at: 1391 3155 1391
Size: 1764 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2022 928
Size: 971 123 4742
Fragments arranged by size:
4742 971 123
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3115 3149 4027 4061 1903
Size: 34 1178 34 878 34 3678
Fragments arranged by size:
3678 1178 878 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2210 3728 4938 2210
Size: 1518 1210 3108
Fragments arranged by size:
3108 1518 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5836
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2093 2420 2743
Size: 107 122 71 631 605 421 327 323
Cuts at: 2743 4447 136
Size: 1704 1525
Fragments arranged by size:
1704 1525 631 605 421 327 323 122
107 71
BglII A'GATC_T
Cuts at: 2262 5831 2262
Size: 3569 2267
BlpI GC'TnA_GC
Cuts at: 2622 3253 2622
Size: 631 5205
Bme1580I G_kGCm'C
Cuts at: 1790 2400 2502 2918 3897 5053 1790
Size: 610 102 416 979 1156 2573
Fragments arranged by size:
2573 1156 979 610 416 102
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5836
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2254 3567 4525
Size: 416 53 83 399 24 837 1313 958
Cuts at: 4525 442
Size: 1753
Fragments arranged by size:
1753 1313 958 837 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5836
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4496 1239
Size: 3257 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3961 4826 5124 3961
Size: 865 298 4673
Fragments arranged by size:
4673 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 2378 2831 4499 882
Size: 617 516 363 453 1668 2219
Fragments arranged by size:
2219 1668 617 516 453 363
BsaAI yAC'GTr
Cuts at: 493 1204 2170 2410 2665 3627 493
Size: 711 966 240 255 962 2702
Fragments arranged by size:
2702 966 962 711 255 240
BsaBI GATnn'nnATC
Cuts at: 2582 2993 3077 5830 2582
Size: 411 84 2753 2588
Fragments arranged by size:
2753 2588 411 84
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2136 2484 3643
Size: 53 83 186 936 552 51 348 1159
Cuts at: 3643 4025 4660 275
Size: 382 635 1451
Fragments arranged by size:
1451 1159 936 635 552 382 348 186
83 53 51
BsaWI w'CCGG_w
Cuts at: 4269 4667 4941 5088 5499 4269
Size: 398 274 147 411 4606
Fragments arranged by size:
4606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2033 2063 2642 2672 1178
Size: 30 165 30 630 30 579 30 4342
Fragments arranged by size:
4342 630 579 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2321 2695 2698 2701 1186
Size: 1135 374 3 3 4321
Fragments arranged by size:
4321 1135 374 3 3
BseYI C'CCAG_C
Cuts at: 2975 5039 5535 2975
Size: 2064 496 3276
Fragments arranged by size:
3276 2064 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5263
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2700 3286 4047 5075 1540
Size: 275 186 699 586 761 1028 2301
Fragments arranged by size:
2301 1028 761 699 586 275 186
BsiHKAI G_wGCw'C
Cuts at: 2229 2313 3897 3982 5053 2229
Size: 84 1584 85 1071 3012
Fragments arranged by size:
3012 1584 1071 85 84
BsmI GAATG_Cn'
Cuts at: 2829 3347 3440 2829
Size: 518 93 5225
Fragments arranged by size:
5225 518 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2780 2780
Size: 5836
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2313 2400 2502 2918 3155 3897
Size: 399 439 84 87 102 416 237 742
Cuts at: 3897 3982 5053 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 416 399 237 102
87 85 84
BspEI T'CCGG_A
Cuts at: 5499 5499
Size: 5836
BspHI T'CATG_A
Cuts at: 2856 3725 5455 2856
Size: 869 1730 3237
Fragments arranged by size:
3237 1730 869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2737 3191 844
Size: 1893 454 3489
Fragments arranged by size:
3489 1893 454
BsrBI CCG'CTC
Cuts at: 1394 1823 3723 1394
Size: 429 1900 3507
Fragments arranged by size:
3507 1900 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4331 4505 65
Size: 1183 3083 174 1396
Fragments arranged by size:
3083 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2066 2456 3104 4480 4667 1700
Size: 110 256 390 648 1376 187 2869
Fragments arranged by size:
2869 1376 648 390 256 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5836
BssSI C'ACGA_G
Cuts at: 2033 2834 3896 4908 2033
Size: 801 1062 1012 2961
Fragments arranged by size:
2961 1062 1012 801
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5836
BstBI TT'CG_AA
Cuts at: 4640 4640
Size: 5836
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5836
BstXI CCAn_nnnn'nTGG
Cuts at: 2410 2410
Size: 5836
BstYI r'GATC_y
Cuts at: 1972 2262 2583 3166 3533 3918 3935 5387 5473
Size: 290 321 583 367 385 17 1452 86
Cuts at: 5473 5485 5831 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 583 385 367 346 321 290
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2983 487
Size: 683 56 1757 3340
Fragments arranged by size:
3340 1757 683 56
BtsI GCAGTG_nn'
Cuts at: 3352 4146 4166 3352
Size: 794 20 5022
Fragments arranged by size:
5022 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5836
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5801
DraI TTT'AAA
Cuts at: 3140 3140
Size: 5836
DraIII CAC_nnn'GTG
Cuts at: 2497 2497
Size: 5836
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2747 4843 809
Size: 507 1431 2096 1802
Fragments arranged by size:
2096 1802 1431 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2412 2697 3283
Size: 54 1425 50 275 18 582 285 586
Cuts at: 3283 4172 5801 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 586 582 285 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2697 3283 1537
Size: 275 885 586 4090
Fragments arranged by size:
4090 885 586 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2798 3766 1353
Size: 1445 968 3423
Fragments arranged by size:
3423 1445 968
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4423 4797 4943 387
Size: 1064 138 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2231 3899 4496 5283 5551 1239
Size: 393 599 1668 597 787 268 1524
Fragments arranged by size:
1668 1524 787 599 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2446 2446
Size: 5836
EcoO109I rG'GnC_Cy
Cuts at: 2332 2884 2332
Size: 552 5284
EcoRI G'AATT_C
Cuts at: 3145 3523 3145
Size: 378 5458
FseI GG_CCGG'CC
Cuts at: 3108 3108
Size: 5836
FspI TGC'GCA
Cuts at: 4342 4342
Size: 5836
HaeII r_GCGC'y
Cuts at: 1678 2773 4983 1678
Size: 1095 2210 2531
Fragments arranged by size:
2531 2210 1095
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2033 2065 4477 4509 4551
Size: 32 135 32 461 32 2412 32 42
Cuts at: 4551 4583 1373
Size: 32 2626
Fragments arranged by size:
2626 2412 461 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3180 3426 669
Size: 988 281 1242 246 3079
Fragments arranged by size:
3079 1242 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5836
HpaI GTT'AAC
Cuts at: 3426 3426
Size: 5836
KpnI G_GTAC'C
Cuts at: 3161 3161
Size: 5836
MfeI C'AATT_G
Cuts at: 3435 3435
Size: 5836
MluI A'CGCG_T
Cuts at: 5712 5712
Size: 5836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4950 5134 1305
Size: 3645 184 2007
Fragments arranged by size:
3645 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2414 10
Size: 54 1425 343 582 3432
Fragments arranged by size:
3432 1425 582 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2408 2813 2921 3794 4153 4312 518
Size: 648 1242 405 108 873 359 159 2042
Fragments arranged by size:
2042 1242 873 648 405 359 159 108
MspA1I CmG'CkG
Cuts at: 1606 2621 2778 3252 3929 5077 5322 1606
Size: 1015 157 474 677 1148 245 2120
Fragments arranged by size:
2120 1148 1015 677 474 245 157
NaeI GCC'GGC
Cuts at: 2068 3106 2068
Size: 1038 4798
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5284
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5836
NgoMIV G'CCGG_C
Cuts at: 2066 3104 2066
Size: 1038 4798
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5836
NotI GC'GGCC_GC
Cuts at: 3283 3283
Size: 5836
NspI r_CATG'y
Cuts at: 1132 1229 2950 3194 4739 1132
Size: 97 1721 244 1545 2229
Fragments arranged by size:
2229 1721 1545 244 97
PasI CC'CwG_GG
Cuts at: 1348 2930 1348
Size: 1582 4254
PciI A'CATG_T
Cuts at: 4735 4735
Size: 5836
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5836
PmeI GTTT'AAAC
Cuts at: 3140 3140
Size: 5836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3905 3937 5418 5450 3905
Size: 32 1481 32 4291
Fragments arranged by size:
4291 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2884 2884
Size: 5836
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5836
PsiI TTA'TAA
Cuts at: 3406 3406
Size: 5836
PstI C_TGCA'G
Cuts at: 830 3188 830
Size: 2358 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5836
PvuII CAG'CTG
Cuts at: 2621 2621
Size: 5836
SalI G'TCGA_C
Cuts at: 1936 3178 1936
Size: 1242 4594
SapI GCTCTTCn'nnn_
Cuts at: 2798 2798
Size: 5836
SbfI CC_TGCA'GG
Cuts at: 3188 3188
Size: 5836
ScaI AGT'ACT
Cuts at: 4084 5796 4084
Size: 1712 4124
SfcI C'TryA_G
Cuts at: 826 1045 3184 4319 5000 5191 826
Size: 219 2139 1135 681 191 1471
Fragments arranged by size:
2139 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2420 2420
Size: 5836
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5836
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5836
SmaI CCC'GGG
Cuts at: 1872 3163 1872
Size: 1291 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3940 4841 5103 820
Size: 197 926 1997 901 262 1553
Fragments arranged by size:
1997 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3627 493
Size: 3134 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5836
SphI G_CATG'C
Cuts at: 1132 2950 3194 1132
Size: 1818 244 3774
Fragments arranged by size:
3774 1818 244
SspI AAT'ATT
Cuts at: 5 52 3760 5
Size: 47 3708 2081
Fragments arranged by size:
3708 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5836
StyI C'CwwG_G
Cuts at: 513 1065 2090 2346 3275 513
Size: 552 1025 256 929 3074
Fragments arranged by size:
3074 1025 929 552 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2504 2989 3863 3880 4033 4218 4663
Size: 1255 924 485 874 17 153 185 445
Cuts at: 4663 325
Size: 1498
Fragments arranged by size:
1498 1255 924 874 485 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4082 5794 96
Size: 274 80 33 51 1443 2105 1712 138
Fragments arranged by size:
2105 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2642 2674 5796 5828 2642
Size: 32 3122 32 2650
Fragments arranged by size:
3122 2650 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2483 1316
Size: 1167 4669
XbaI T'CTAG_A
Cuts at: 3172 3172
Size: 5836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2539 2539
Size: 5836
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5836
XmaI C'CCGG_G
Cuts at: 1870 3161 1870
Size: 1291 4545
XmnI GAAnn'nnTTC
Cuts at: 3965 3965
Size: 5836
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3644 276
Size: 53 83 186 936 2110 2468
Fragments arranged by size:
2468 2110 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfoI SrfI SwaI