Restriction map for
pFN21AB6578
(Circular) (Six-base) MAPSORT of: pFN21AB6578.seq Check: 4592 from: 1 to: 5911
pFN21AB6578 5911 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2931 2931
Size: 5911
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2277 3721 278
Size: 53 83 186 936 741 1444 2468
Fragments arranged by size:
2468 1444 936 741 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3254 1937
Size: 1317 4594
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 4038 4411 1563
Size: 2475 373 3063
Fragments arranged by size:
3063 2475 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2749 2898 3974 5358 5626 1632
Size: 1117 149 1076 1384 268 1917
Fragments arranged by size:
1917 1384 1117 1076 268 149
AfeI AGC'GCT
Cuts at: 2807 2807
Size: 5911
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5714
AflIII A'CryG_T
Cuts at: 1203 4810 5787 1203
Size: 3607 977 1327
Fragments arranged by size:
3607 1327 977
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5911
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2319 2351 2319
Size: 32 5879
AlwNI CAG_nnn'CTG
Cuts at: 2089 2949 3097 3654 5226 2089
Size: 860 148 557 1572 2774
Fragments arranged by size:
2774 1572 860 557 148
ApaI G_GGCC'C
Cuts at: 2052 2422 3056 3162 2052
Size: 370 634 106 4801
Fragments arranged by size:
4801 634 370 106
ApaLI G'TGCA_C
Cuts at: 2447 2626 3968 5124 2447
Size: 179 1342 1156 3234
Fragments arranged by size:
3234 1342 1156 179
ApoI r'AATT_y
Cuts at: 1493 3220 3446 3598 5707 1493
Size: 1727 226 152 2109 1697
Fragments arranged by size:
2109 1727 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 2051 3154 3236 1870
Size: 73 108 1103 82 4545
Fragments arranged by size:
4545 1103 108 82 73
BamHI G'GATC_C
Cuts at: 2587 3241 3608 2587
Size: 654 367 4890
Fragments arranged by size:
4890 654 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2163 3232 4687 618
Size: 325 217 627 376 1069 1455 1842
Fragments arranged by size:
1842 1455 1069 627 376 325 217
BanII G_rGCy'C
Cuts at: 1391 2052 2422 2639 2859 3036 3056 3162 3230
Size: 661 370 217 220 177 20 106 68
Cuts at: 3230 1391
Size: 4072
Fragments arranged by size:
4072 661 370 220 217 177 106 68
20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2974 928
Size: 971 1075 3865
Fragments arranged by size:
3865 1075 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4102 4136 1903
Size: 34 2165 34 3678
Fragments arranged by size:
3678 2165 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 2995 1548
Size: 1447 4464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2499 2958 3040
Size: 107 122 71 631 605 827 459 82
Cuts at: 3040 4522 136
Size: 1482 1525
Fragments arranged by size:
1525 1482 827 631 605 459 122 107
82 71
BglII A'GATC_T
Cuts at: 5906 5906
Size: 5911
BlpI GC'TnA_GC
Cuts at: 3328 3328
Size: 5911
Bme1580I G_kGCm'C
Cuts at: 1790 2052 2166 2422 2451 2630 3056 3139 3162
Size: 262 114 256 29 179 426 83 23
Cuts at: 3162 3972 5128 1790
Size: 810 1156 2573
Fragments arranged by size:
2573 1156 810 426 262 256 179 114
83 29 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5911
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2461 3164 3642
Size: 416 53 83 399 24 1044 703 478
Cuts at: 3642 4600 442
Size: 958 1753
Fragments arranged by size:
1753 1044 958 703 478 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5911
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3133 3133
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2510 4571 1239
Size: 1271 2061 2579
Fragments arranged by size:
2579 2061 1271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4036 4901 5199 4036
Size: 865 298 4748
Fragments arranged by size:
4748 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2682 4574 882
Size: 617 1183 1892 2219
Fragments arranged by size:
2219 1892 1183 617
BsaAI yAC'GTr
Cuts at: 493 1204 3702 493
Size: 711 2498 2702
Fragments arranged by size:
2702 2498 711
BsaBI GATnn'nnATC
Cuts at: 5905 5905
Size: 5911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2274 3718 4100 4735
Size: 53 83 186 936 741 1444 382 635
Cuts at: 4735 275
Size: 1451
Fragments arranged by size:
1451 1444 936 741 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4344 4742 5016 5163 5574 4344
Size: 398 274 147 411 4681
Fragments arranged by size:
4681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2369 2399 1178
Size: 30 165 30 966 30 4690
Fragments arranged by size:
4690 966 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2210 2371 2390 2426 1186
Size: 1012 12 161 19 36 4671
Fragments arranged by size:
4671 1012 161 36 19 12
BseYI C'CCAG_C
Cuts at: 3055 3060 3064 5114 5610 3055
Size: 5 4 2050 496 3356
Fragments arranged by size:
3356 2050 496 5 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2337 2811 1163
Size: 573 601 474 4263
Fragments arranged by size:
4263 601 573 474
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2152 3361 4122 5150 1540
Size: 275 186 151 1209 761 1028 2301
Fragments arranged by size:
2301 1209 1028 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 2451 2630 3132 3972 4057 5128 2451
Size: 179 502 840 85 1071 3234
Fragments arranged by size:
3234 1071 840 502 179 85
BsmI GAATG_Cn'
Cuts at: 3422 3515 3422
Size: 93 5818
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2166 2422 2451 2630 2639 2859
Size: 399 262 114 256 29 179 9 220
Cuts at: 2859 3036 3056 3132 3139 3162 3230 3972 4057
Size: 177 20 76 7 23 68 742 85
Cuts at: 4057 5128 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 262 256 220 179
177 114 85 76 68 29 23 20
9 7
BspEI T'CCGG_A
Cuts at: 5574 5574
Size: 5911
BspHI T'CATG_A
Cuts at: 3800 5530 3800
Size: 1730 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2931 3076 3266 844
Size: 2087 145 190 3489
Fragments arranged by size:
3489 2087 190 145
BsrBI CCG'CTC
Cuts at: 1394 1823 2416 3798 1394
Size: 429 593 1382 3507
Fragments arranged by size:
3507 1382 593 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4406 4580 65
Size: 1183 3158 174 1396
Fragments arranged by size:
3158 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4555 4742 1700
Size: 110 2745 187 2869
Fragments arranged by size:
2869 2745 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5911
BssHII G'CGCG_C
Cuts at: 1925 2188 1925
Size: 263 5648
BssSI C'ACGA_G
Cuts at: 3029 3971 4983 3029
Size: 942 1012 3957
Fragments arranged by size:
3957 1012 942
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2949 794
Size: 2155 3756
BstBI TT'CG_AA
Cuts at: 4715 4715
Size: 5911
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5911
BstXI CCAn_nnnn'nTGG
Cuts at: 2266 2830 2266
Size: 564 5347
BstYI r'GATC_y
Cuts at: 1972 2587 3241 3608 3993 4010 5462 5548 5560
Size: 615 654 367 385 17 1452 86 12
Cuts at: 5560 5906 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 654 615 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5359
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5172
Fragments arranged by size:
5172 683 56
BtsI GCAGTG_nn'
Cuts at: 2307 2950 3427 4221 4241 2307
Size: 643 477 794 20 3977
Fragments arranged by size:
3977 794 643 477 20
ClaI AT'CG_AT
Cuts at: 1554 2706 1554
Size: 1152 4759
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5876
DraI TTT'AAA
Cuts at: 3215 3215
Size: 5911
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2080 2310 3183
Size: 54 1425 50 275 18 250 230 873
Cuts at: 3183 3358 4247 5876 8
Size: 175 889 1629 43
Fragments arranged by size:
1629 1425 889 873 275 250 230 175
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3358 1537
Size: 275 1546 4090
Fragments arranged by size:
4090 1546 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2403 3107 3170 3841 1353
Size: 1050 704 63 671 3423
Fragments arranged by size:
3423 1050 704 671 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 1999 2162 2195 2285 4498 4872
Size: 1064 138 410 163 33 90 2213 374
Cuts at: 4872 5018 387
Size: 146 1280
Fragments arranged by size:
2213 1280 1064 410 374 163 146 138
90 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 2749 2898 3974 4571 5358 5626
Size: 393 878 239 149 1076 597 787 268
Cuts at: 5626 1239
Size: 1524
Fragments arranged by size:
1524 1076 878 787 597 393 268 239
149
EcoO109I rG'GnC_Cy
Cuts at: 3052 3158 3159 3052
Size: 106 1 5804
Fragments arranged by size:
5804 106 1
EcoRI G'AATT_C
Cuts at: 3220 3598 3220
Size: 378 5533
FspI TGC'GCA
Cuts at: 4417 4417
Size: 5911
HaeII r_GCGC'y
Cuts at: 1678 2465 2809 5058 1678
Size: 787 344 2249 2531
Fragments arranged by size:
2531 2249 787 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4552 4584 4626 4658 1373
Size: 32 135 32 2980 32 42 32 2626
Fragments arranged by size:
2980 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3255 3501 669
Size: 988 281 1317 246 3079
Fragments arranged by size:
3079 1317 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5911
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 2735 3510 2735
Size: 775 5136
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2580 5025 5209 1305
Size: 1275 2445 184 2007
Fragments arranged by size:
2445 2007 1275 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2312 10
Size: 54 1425 343 480 3609
Fragments arranged by size:
3609 1425 480 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2517 3869 4228 4387 518
Size: 648 1351 1352 359 159 2042
Fragments arranged by size:
2042 1352 1351 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2341 2383 2443 2530 3064 3327 4004 5152
Size: 735 42 60 87 534 263 677 1148
Cuts at: 5152 5397 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 735 677 534 263 245 87
60 42
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5359
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5911
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5911
NotI GC'GGCC_GC
Cuts at: 3358 3358
Size: 5911
NspI r_CATG'y
Cuts at: 1132 1229 2506 3269 4814 1132
Size: 97 1277 763 1545 2229
Fragments arranged by size:
2229 1545 1277 763 97
PasI CC'CwG_GG
Cuts at: 1348 3037 1348
Size: 1689 4222
PciI A'CATG_T
Cuts at: 4810 4810
Size: 5911
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5911
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5493 5525 3980
Size: 32 1481 32 4366
Fragments arranged by size:
4366 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2952 1613
Size: 1339 4572
PsiI TTA'TAA
Cuts at: 3481 3481
Size: 5911
PspOMI G'GGCC_C
Cuts at: 2048 2418 3052 3158 2048
Size: 370 634 106 4801
Fragments arranged by size:
4801 634 370 106
PstI C_TGCA'G
Cuts at: 830 2238 2469 3263 830
Size: 1408 231 794 3478
Fragments arranged by size:
3478 1408 794 231
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
PvuII CAG'CTG
Cuts at: 2530 3064 2530
Size: 534 5377
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SapI GCTCTTCn'nnn_
Cuts at: 3107 3107
Size: 5911
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SfcI C'TryA_G
Cuts at: 826 1045 2234 2465 3259 4394 5075 5266 826
Size: 219 1189 231 794 1135 681 191 1471
Fragments arranged by size:
1471 1189 1135 794 681 231 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5911
SmaI CCC'GGG
Cuts at: 1872 2053 3156 3238 1872
Size: 181 1103 82 4545
Fragments arranged by size:
4545 1103 181 82
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4015 4916 5178 820
Size: 197 926 2072 901 262 1553
Fragments arranged by size:
2072 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 2506 3269 1132
Size: 1374 763 3774
Fragments arranged by size:
3774 1374 763
SrfI GCCC'GGGC
Cuts at: 2053 2053
Size: 5911
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 2786 1517
Size: 1269 4642
StyI C'CwwG_G
Cuts at: 513 1065 3350 513
Size: 552 2285 3074
Fragments arranged by size:
3074 2285 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2579 3056 3938 3955 4108 4293 4738
Size: 1255 999 477 882 17 153 185 445
Cuts at: 4738 325
Size: 1498
Fragments arranged by size:
1498 1255 999 882 477 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4157 5869 96
Size: 274 80 33 51 1443 2180 1712 138
Fragments arranged by size:
2180 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5871 5903 5871
Size: 32 5879
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5911
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5911
XmaI C'CCGG_G
Cuts at: 1870 2051 3154 3236 1870
Size: 181 1103 82 4545
Fragments arranged by size:
4545 1103 181 82
XmnI GAAnn'nnTTC
Cuts at: 4040 4040
Size: 5911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2275 3719 276
Size: 53 83 186 936 741 1444 2468
Fragments arranged by size:
2468 1444 936 741 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SwaI XcmI