Restriction map for
pFN21AB6572
(Circular) (Six-base) MAPSORT of: pFN21AB6572.seq Check: 7083 from: 1 to: 5905
pFN21AB6572 5905 bp
With 182 enzymes: SgfI *
April 15, 2010 09:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2265 3248 1937
Size: 328 983 4594
Fragments arranged by size:
4594 983 328
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 2565 2780 3968 5352 5620 1632
Size: 557 376 215 1188 1384 268 1917
Fragments arranged by size:
1917 1384 1188 557 376 268 215
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 3081 4804 5781 1203
Size: 1878 1723 977 1327
Fragments arranged by size:
1878 1723 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 2246 2396 1166
Size: 1080 150 4675
Fragments arranged by size:
4675 1080 150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2180 1184
Size: 996 4909
AlwNI CAG_nnn'CTG
Cuts at: 2778 3648 5220 2778
Size: 870 1572 3463
Fragments arranged by size:
3463 1572 870
ApaI G_GGCC'C
Cuts at: 2182 2946 2182
Size: 764 5141
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 2293 2854 3214 3440 3592 5701 1493
Size: 800 561 360 226 152 2109 1697
Fragments arranged by size:
2109 1697 800 561 360 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 2955 3230 1870
Size: 73 1012 275 4545
Fragments arranged by size:
4545 1012 275 73
BamHI G'GATC_C
Cuts at: 2670 3235 3602 2670
Size: 565 367 4973
Fragments arranged by size:
4973 565 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 3226 4681 618
Size: 325 217 627 282 1157 1455 1842
Fragments arranged by size:
1842 1455 1157 627 325 282 217
BanII G_rGCy'C
Cuts at: 1391 2182 2441 2946 3224 1391
Size: 791 259 505 278 4072
Fragments arranged by size:
4072 791 505 278 259
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2128 2852 928
Size: 971 229 724 3981
Fragments arranged by size:
3981 971 724 229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 2463 1548
Size: 915 4990
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 4516 136
Size: 107 122 71 631 605 406 2438 1525
Fragments arranged by size:
2438 1525 631 605 406 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 2885 3322 2885
Size: 437 5468
Bme1580I G_kGCm'C
Cuts at: 1790 2182 2946 3966 5122 1790
Size: 392 764 1020 1156 2573
Fragments arranged by size:
2573 1156 1020 764 392
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3636 4594 442
Size: 416 53 83 399 24 2219 958 1753
Fragments arranged by size:
2219 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2774 4565 1239
Size: 1052 483 1791 2579
Fragments arranged by size:
2579 1791 1052 483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3173 4030 4895 5193 3173
Size: 857 865 298 3885
Fragments arranged by size:
3885 865 857 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4568 882
Size: 617 3069 2219
Fragments arranged by size:
3069 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3082 3124 3696 493
Size: 711 1878 42 572 2702
Fragments arranged by size:
2702 1878 711 572 42
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3712 4094 4729 275
Size: 53 83 186 936 2179 382 635 1451
Fragments arranged by size:
2179 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3174 4338 4736 5010 5157 5568 3174
Size: 1164 398 274 147 411 3511
Fragments arranged by size:
3511 1164 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2601 2631 2719 2749 1178
Size: 30 165 30 1198 30 88 30 4334
Fragments arranged by size:
4334 1198 165 88 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2835 2838 2855 1186
Size: 1649 3 17 4236
Fragments arranged by size:
4236 1649 17 3
BseYI C'CCAG_C
Cuts at: 2938 3115 5108 5604 2938
Size: 177 1993 496 3239
Fragments arranged by size:
3239 1993 496 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5332
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2472 3355 4116 5144 1540
Size: 275 186 471 883 761 1028 2301
Fragments arranged by size:
2301 1028 883 761 471 275 186
BsiHKAI G_wGCw'C
Cuts at: 2441 3966 4051 5122 2441
Size: 1525 85 1071 3224
Fragments arranged by size:
3224 1525 1071 85
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
BsmBI CGTCTCn'nnnn_
Cuts at: 2653 2653
Size: 5905
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2182 2441 2946 3224 3966 4051 5122
Size: 399 392 259 505 278 742 85 1071
Cuts at: 5122 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 505 399 392 278 259
85
BspEI T'CCGG_A
Cuts at: 3174 5568 3174
Size: 2394 3511
BspHI T'CATG_A
Cuts at: 2337 2460 3794 5524 2337
Size: 123 1334 1730 2718
Fragments arranged by size:
2718 1730 1334 123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2607 3260 844
Size: 1763 653 3489
Fragments arranged by size:
3489 1763 653
BsrBI CCG'CTC
Cuts at: 1394 1823 2176 3792 1394
Size: 429 353 1616 3507
Fragments arranged by size:
3507 1616 429 353
BsrDI GCAATG_nn'
Cuts at: 65 1248 2149 2917 4400 4574 65
Size: 1183 901 768 1483 174 1396
Fragments arranged by size:
1483 1396 1183 901 768 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4549 4736 1700
Size: 110 2739 187 2869
Fragments arranged by size:
2869 2739 187 110
BsrGI T'GTAC_A
Cuts at: 96 2345 96
Size: 2249 3656
BssHII G'CGCG_C
Cuts at: 1925 2115 1925
Size: 190 5715
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 2899 4709 2899
Size: 1810 4095
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstXI CCAn_nnnn'nTGG
Cuts at: 2027 2027
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2670 3192 3235 3602 3987 4004 5456 5542
Size: 698 522 43 367 385 17 1452 86
Cuts at: 5542 5554 5900 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 698 522 385 367 346 86
43 17 12
BstZ17I GTA'TAC
Cuts at: 2266 2266
Size: 5905
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4414
Fragments arranged by size:
4414 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 2391 2403 2787 3064 3421 4215 4235 2391
Size: 12 384 277 357 794 20 4061
Fragments arranged by size:
4061 794 384 357 277 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2540 2688 4912 809
Size: 507 1224 148 2224 1802
Fragments arranged by size:
2224 1802 1224 507 148
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2127 2238
Size: 54 1425 50 275 18 199 98 111
Cuts at: 2238 2469 2997 3352 4241 5870 8
Size: 231 528 355 889 1629 43
Fragments arranged by size:
1629 1425 889 528 355 275 231 199
111 98 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2469 3352 1537
Size: 275 657 883 4090
Fragments arranged by size:
4090 883 657 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2511 2584 3835 1353
Size: 1158 73 1251 3423
Fragments arranged by size:
3423 1251 1158 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4492 4866 5012 387
Size: 1064 138 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2439 2439
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2291 2565 2774 2780 3968 4565
Size: 393 557 102 274 209 6 1188 597
Cuts at: 4565 5352 5620 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1188 787 597 557 393 274 268
209 102 6
EcoO109I rG'GnC_Cy
Cuts at: 2179 2533 2942 2943 3012 2179
Size: 354 409 1 69 5072
Fragments arranged by size:
5072 409 354 69 1
EcoRI G'AATT_C
Cuts at: 2854 3214 3592 2854
Size: 360 378 5167
Fragments arranged by size:
5167 378 360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 5905
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2879 3135 5052 1678
Size: 1201 256 1917 2531
Fragments arranged by size:
2531 1917 1201 256
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2428 2460 2601 2633 2662
Size: 32 135 32 856 32 141 32 29
Cuts at: 2662 2694 4546 4578 4620 4652 1373
Size: 32 1852 32 42 32 2626
Fragments arranged by size:
2626 1852 856 141 135 42 32 32
32 32 32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 3249 3495 669
Size: 988 281 1311 246 3079
Fragments arranged by size:
3079 1311 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2580 5019 5203 1305
Size: 1275 2439 184 2007
Fragments arranged by size:
2439 2007 1275 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2031 2129 2240 2999 10
Size: 54 1425 343 199 98 111 759 2916
Fragments arranged by size:
2916 1425 759 343 199 111 98 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2246 2396 3863 4222 4381 518
Size: 648 996 84 150 1467 359 159 2042
Fragments arranged by size:
2042 1467 996 648 359 159 150 84
MspA1I CmG'CkG
Cuts at: 1606 2023 3321 3998 5146 5391 1606
Size: 417 1298 677 1148 245 2120
Fragments arranged by size:
2120 1298 1148 677 417 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4414
Fragments arranged by size:
4414 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 2093 3078 3263 4808 1132
Size: 97 864 985 185 1545 2229
Fragments arranged by size:
2229 1545 985 864 185 97
PasI CC'CwG_GG
Cuts at: 1348 2256 1348
Size: 908 4997
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2995 3111 1108
Size: 1887 116 3902
Fragments arranged by size:
3902 1887 116
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PmlI CAC'GTG
Cuts at: 3082 3082
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2533 2533
Size: 5905
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2178 2942 2178
Size: 764 5141
PstI C_TGCA'G
Cuts at: 830 2187 2398 3257 830
Size: 1357 211 859 3478
Fragments arranged by size:
3478 1357 859 211
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 2023 2023
Size: 5905
SacI G_AGCT'C
Cuts at: 2441 2441
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SanDI GG'GwC_CC
Cuts at: 2533 2533
Size: 5905
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 4153 5865 4153
Size: 1712 4193
SfcI C'TryA_G
Cuts at: 826 1045 2183 2394 2770 3253 4388 5069 5260
Size: 219 1138 211 376 483 1135 681 191
Cuts at: 5260 826
Size: 1471
Fragments arranged by size:
1471 1138 1135 681 483 376 219 211
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3188 4009 4910 5172 820
Size: 197 926 1245 821 901 262 1553
Fragments arranged by size:
1553 1245 926 901 821 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 2710 1517
Size: 1193 4712
StyI C'CwwG_G
Cuts at: 513 1065 2004 2244 2793 3344 513
Size: 552 939 240 549 551 3074
Fragments arranged by size:
3074 939 552 551 549 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2248 2786 3932 3949 4102 4287 4732
Size: 1255 668 538 1146 17 153 185 445
Cuts at: 4732 325
Size: 1498
Fragments arranged by size:
1498 1255 1146 668 538 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2345 4151 5863
Size: 274 80 33 51 1443 368 1806 1712
Cuts at: 5863 96
Size: 138
Fragments arranged by size:
1806 1712 1443 368 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5865 5897 5865
Size: 32 5873
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2638 3112 2638
Size: 474 5431
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 3182 4034 3182
Size: 852 5053
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI Bsu36I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspXI PsrI RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI