Restriction map for
pFN21AB6570
(Circular) (Six-base) MAPSORT of: pFN21AB6570.seq Check: 5136 from: 1 to: 5905
pFN21AB6570 5905 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2810 3025 2810
Size: 215 5690
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2427 3248 1937
Size: 490 821 4594
Fragments arranged by size:
4594 821 490
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3044 3050 3968 5352 5620 1632
Size: 1412 6 918 1384 268 1917
Fragments arranged by size:
1917 1412 1384 918 268 6
AfeI AGC'GCT
Cuts at: 2614 2614
Size: 5905
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 3015 4804 5781 1203
Size: 1812 1789 977 1327
Fragments arranged by size:
1812 1789 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 4634 4634
Size: 5905
AleI CACnn'nnGTG
Cuts at: 1166 2900 3125 1166
Size: 1734 225 3946
Fragments arranged by size:
3946 1734 225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AlwNI CAG_nnn'CTG
Cuts at: 2481 3648 5220 2481
Size: 1167 1572 3166
Fragments arranged by size:
3166 1572 1167
ApaI G_GGCC'C
Cuts at: 2927 2927
Size: 5905
ApaLI G'TGCA_C
Cuts at: 2333 2867 3962 5118 2333
Size: 534 1095 1156 3120
Fragments arranged by size:
3120 1156 1095 534
ApoI r'AATT_y
Cuts at: 1493 3214 3440 3592 5701 1493
Size: 1721 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 2567 3230 1870
Size: 73 624 663 4545
Fragments arranged by size:
4545 663 624 73
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2701 3226 4681 618
Size: 325 217 627 914 525 1455 1842
Fragments arranged by size:
1842 1455 914 627 525 325 217
BanII G_rGCy'C
Cuts at: 1391 2471 2927 3224 1391
Size: 1080 456 297 4072
Fragments arranged by size:
4072 1080 456 297
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4934
BbvCI CC'TCA_GC
Cuts at: 2321 2383 2321
Size: 62 5843
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3797 5007 3797
Size: 1210 4695
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4516 136
Size: 107 122 71 631 605 2844 1525
Fragments arranged by size:
2844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5900 5900
Size: 5905
BlpI GC'TnA_GC
Cuts at: 3060 3322 3060
Size: 262 5643
Bme1580I G_kGCm'C
Cuts at: 1790 2337 2706 2871 2927 3966 5122 1790
Size: 547 369 165 56 1039 1156 2573
Fragments arranged by size:
2573 1156 1039 547 369 165 56
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2083 3636 4594
Size: 416 53 83 399 24 666 1553 958
Cuts at: 4594 442
Size: 1753
Fragments arranged by size:
1753 1553 958 666 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2145 2145
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2299 4565 1239
Size: 1060 2266 2579
Fragments arranged by size:
2579 2266 1060
Bpu10I CC'TnA_GC
Cuts at: 2120 2321 2383 2470 2120
Size: 201 62 87 5555
Fragments arranged by size:
5555 201 87 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2657 4568 882
Size: 617 1158 1911 2219
Fragments arranged by size:
2219 1911 1158 617
BsaAI yAC'GTr
Cuts at: 493 1204 2338 3696 493
Size: 711 1134 1358 2702
Fragments arranged by size:
2702 1358 1134 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3712 4094 4729 275
Size: 53 83 186 936 2179 382 635 1451
Fragments arranged by size:
2179 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2193 4338 4736 5010 5157 5568 2193
Size: 2145 398 274 147 411 2530
Fragments arranged by size:
2530 2145 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5680
Fragments arranged by size:
5680 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2104 2231 2371 1186
Size: 918 127 140 4720
Fragments arranged by size:
4720 918 140 127
BseYI C'CCAG_C
Cuts at: 2753 2763 5108 5604 2753
Size: 10 2345 496 3054
Fragments arranged by size:
3054 2345 496 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2117 1163
Size: 573 381 4951
Fragments arranged by size:
4951 573 381
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2337 2598 2871 3966 4051 5122 2337
Size: 261 273 1095 85 1071 3120
Fragments arranged by size:
3120 1095 1071 273 261 85
BsmI GAATG_Cn'
Cuts at: 3416 3509 3416
Size: 93 5812
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 2471 2598 2706 2871 2927 3224
Size: 399 547 134 127 108 165 56 297
Cuts at: 3224 3966 4051 5122 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 547 399 297 165 134
127 108 85 56
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2810 3025 3260 844
Size: 1966 215 235 3489
Fragments arranged by size:
3489 1966 235 215
BsrBI CCG'CTC
Cuts at: 1394 1823 3792 1394
Size: 429 1969 3507
Fragments arranged by size:
3507 1969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4400 4574 65
Size: 1183 3152 174 1396
Fragments arranged by size:
3152 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2047 3043 4549 4736 1700
Size: 110 237 996 1506 187 2869
Fragments arranged by size:
2869 1506 996 237 187 110
BsrGI T'GTAC_A
Cuts at: 96 2039 96
Size: 1943 3962
BssHII G'CGCG_C
Cuts at: 1925 2708 1925
Size: 783 5122
BssSI C'ACGA_G
Cuts at: 3965 4977 3965
Size: 1012 4893
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2481 794
Size: 1687 4218
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5905
BstYI r'GATC_y
Cuts at: 1972 2259 3235 3602 3987 4004 5456 5542 5554
Size: 287 976 367 385 17 1452 86 12
Cuts at: 5554 5900 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 976 385 367 346 287 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2234 2361 2640 2697 2887 3081
Size: 552 939 230 127 279 57 190 194
Cuts at: 3081 513
Size: 3337
Fragments arranged by size:
3337 939 552 279 230 194 190 127
57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2041 2100 2533 487
Size: 683 56 815 59 433 3859
Fragments arranged by size:
3859 815 683 433 59 56
BtsI GCAGTG_nn'
Cuts at: 3421 4215 4235 3421
Size: 794 20 5091
Fragments arranged by size:
5091 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DraIII CAC_nnn'GTG
Cuts at: 2866 2866
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2209 3078 3352
Size: 54 1425 50 275 18 379 869 274
Cuts at: 3352 4241 5870 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 869 379 275 274 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2085 3835 1353
Size: 732 1750 3423
Fragments arranged by size:
3423 1750 732
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2732 4492 4866 5012 387
Size: 1064 138 1143 1760 374 146 1280
Fragments arranged by size:
1760 1280 1143 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2299 3044 3050 3968 4565 5352 5620
Size: 393 667 745 6 918 597 787 268
Cuts at: 5620 1239
Size: 1524
Fragments arranged by size:
1524 918 787 745 667 597 393 268
6
EcoO109I rG'GnC_Cy
Cuts at: 2302 2924 3137 3176 2302
Size: 622 213 39 5031
Fragments arranged by size:
5031 622 213 39
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FseI GG_CCGG'CC
Cuts at: 3047 3047
Size: 5905
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 2048 2481 2616 5052 1678
Size: 370 433 135 2436 2531
Fragments arranged by size:
2531 2436 433 370 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373
Size: 32 135 32 2974 32 42 32 2626
Fragments arranged by size:
2974 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2053 3249 3495 669
Size: 988 281 115 1196 246 3079
Fragments arranged by size:
3079 1196 988 281 246 115
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5019 5203 1305
Size: 3714 184 2007
Fragments arranged by size:
3714 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3080 10
Size: 54 1425 343 1248 2835
Fragments arranged by size:
2835 1425 1248 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 2900 3125 3863 4222 4381 518
Size: 648 1437 297 225 738 359 159 2042
Fragments arranged by size:
2042 1437 738 648 359 297 225 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2236 2265 2308 2325 2387 2730 2767
Size: 417 213 29 43 17 62 343 37
Cuts at: 2767 3059 3200 3321 3998 5146 5391 1606
Size: 292 141 121 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 417 343 292 245 213
141 121 62 43 37 29 17
NaeI GCC'GGC
Cuts at: 3045 3045
Size: 5905
NcoI C'CATG_G
Cuts at: 513 1065 2004 3081 513
Size: 552 939 1077 3337
Fragments arranged by size:
3337 1077 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5905
NgoMIV G'CCGG_C
Cuts at: 3043 3043
Size: 5905
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 2650 3263 4808 1132
Size: 97 1421 613 1545 2229
Fragments arranged by size:
2229 1545 1421 613 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 4804 4804
Size: 5905
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2847 3172 1108
Size: 1739 325 3841
Fragments arranged by size:
3841 1739 325
PfoI T'CCnGG_A
Cuts at: 2736 3147 2736
Size: 411 5494
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PmlI CAC'GTG
Cuts at: 2338 2338
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2302 3176 2302
Size: 874 5031
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PspOMI G'GGCC_C
Cuts at: 2923 2923
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2244 3036 3257 830
Size: 1414 792 221 3478
Fragments arranged by size:
3478 1414 792 221
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 2325 2767 3059 3200 2325
Size: 442 292 141 5030
Fragments arranged by size:
5030 442 292 141
SacII CC_GC'GG
Cuts at: 2237 2237
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SanDI GG'GwC_CC
Cuts at: 2302 2302
Size: 5905
SapI GCTCTTCn'nnn_
Cuts at: 2085 2085
Size: 5905
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 2072 4153 5865 2072
Size: 2081 1712 2112
Fragments arranged by size:
2112 2081 1712
SexAI A'CCwGG_T
Cuts at: 2862 2862
Size: 5905
SfcI C'TryA_G
Cuts at: 826 1045 2240 3032 3253 4388 5069 5260 826
Size: 219 1195 792 221 1135 681 191 1471
Fragments arranged by size:
1471 1195 1135 792 681 221 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4009 4910 5172 820
Size: 197 926 2066 901 262 1553
Fragments arranged by size:
2066 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 2650 3263 1132
Size: 1518 613 3774
Fragments arranged by size:
3774 1518 613
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 2246 1517
Size: 729 5176
StyI C'CwwG_G
Cuts at: 513 1065 2004 2898 3081 3344 513
Size: 552 939 894 183 263 3074
Fragments arranged by size:
3074 939 894 552 263 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3932 3949 4102 4287 4732 325
Size: 1255 2352 17 153 185 445 1498
Fragments arranged by size:
2352 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2039 2070 4151
Size: 274 80 33 51 1443 62 31 2081
Cuts at: 4151 5863 96
Size: 1712 138
Fragments arranged by size:
2081 1712 1443 274 138 80 62 51
33 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5865 5897 5865
Size: 32 5873
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3241 3241
Size: 5905
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 2354 4034 2354
Size: 1680 4225
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacI SfiI SfoI SrfI SwaI XcmI