Restriction map for
pFN21AB6562
(Circular) (Six-base) MAPSORT of: pFN21AB6562.seq Check: 2338 from: 1 to: 5902
pFN21AB6562 5902 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2549 3245 1937
Size: 612 696 4594
Fragments arranged by size:
4594 696 612
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2156 2285 2494 3965 5349 5617 1632
Size: 524 129 209 1471 1384 268 1917
Fragments arranged by size:
1917 1471 1384 524 268 209 129
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 4801 5778 1203
Size: 3598 977 1327
Fragments arranged by size:
3598 1327 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 3645 5217 3645
Size: 1572 4330
ApaLI G'TGCA_C
Cuts at: 2502 3959 5115 2502
Size: 1457 1156 3289
Fragments arranged by size:
3289 1457 1156
ApoI r'AATT_y
Cuts at: 1493 2143 2400 2613 3008 3211 3437 3589 5698
Size: 650 257 213 395 203 226 152 2109
Cuts at: 5698 1493
Size: 1697
Fragments arranged by size:
2109 1697 650 395 257 226 213 203
152
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 3016 3227 1870
Size: 73 77 996 211 4545
Fragments arranged by size:
4545 996 211 77 73
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2086 2815 3223 4678
Size: 325 217 627 273 26 729 408 1455
Cuts at: 4678 618
Size: 1842
Fragments arranged by size:
1842 1455 729 627 408 325 273 217
26
BanII G_rGCy'C
Cuts at: 1391 2021 3221 1391
Size: 630 1200 4072
Fragments arranged by size:
4072 1200 630
BbeI G_GCGC'C
Cuts at: 2090 2090
Size: 5902
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4931
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 2947 1548
Size: 1399 4503
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2025 4513
Size: 107 122 71 631 605 337 16 2488
Cuts at: 4513 136
Size: 1525
Fragments arranged by size:
2488 1525 631 605 337 122 107 71
16
BglII A'GATC_T
Cuts at: 2268 5897 2268
Size: 3629 2273
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 2506 3963 5119 1790
Size: 716 1457 1156 2573
Fragments arranged by size:
2573 1457 1156 716
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2296 3633 4591
Size: 416 53 83 399 24 879 1337 958
Cuts at: 4591 442
Size: 1753
Fragments arranged by size:
1753 1337 958 879 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2896 4562 1239
Size: 1657 1666 2579
Fragments arranged by size:
2579 1666 1657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4027 4892 5190 4027
Size: 865 298 4739
Fragments arranged by size:
4739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 882
Size: 617 3066 2219
Fragments arranged by size:
3066 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3693 493
Size: 711 2489 2702
Fragments arranged by size:
2702 2489 711
BsaBI GATnn'nnATC
Cuts at: 5896 5896
Size: 5902
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2087 3709 4091 4726
Size: 53 83 186 936 554 1622 382 635
Cuts at: 4726 275
Size: 1451
Fragments arranged by size:
1622 1451 936 635 554 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4335 4733 5007 5154 5565 4335
Size: 398 274 147 411 4672
Fragments arranged by size:
4672 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5677
Fragments arranged by size:
5677 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2349 1186
Size: 1163 4739
BseYI C'CCAG_C
Cuts at: 3179 5105 5601 3179
Size: 1926 496 3480
Fragments arranged by size:
3480 1926 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2103 3352 4113 5141 1540
Size: 275 186 102 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186 102
BsiHKAI G_wGCw'C
Cuts at: 2506 3963 4048 5119 2506
Size: 1457 85 1071 3289
Fragments arranged by size:
3289 1457 1071 85
BsmI GAATG_Cn'
Cuts at: 3413 3506 3413
Size: 93 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2506 3221 3963 4048 5119 1391
Size: 399 231 485 715 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 715 485 399 231 85
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3257 844
Size: 2413 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2025 2865 3789 1394
Size: 429 202 840 924 3507
Fragments arranged by size:
3507 924 840 429 202
BsrDI GCAATG_nn'
Cuts at: 65 1248 4397 4571 65
Size: 1183 3149 174 1396
Fragments arranged by size:
3149 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4546 4733 1700
Size: 110 2736 187 2869
Fragments arranged by size:
2869 2736 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 4706 4706
Size: 5902
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2268 2529 2974 3232 3599 3984 4001 5453
Size: 296 261 445 258 367 385 17 1452
Cuts at: 5453 5539 5551 5897 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 445 385 367 346 296 261
258 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4411
Fragments arranged by size:
4411 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5163
Fragments arranged by size:
5163 683 56
BtsI GCAGTG_nn'
Cuts at: 2295 3418 4212 4232 2295
Size: 1123 794 20 3965
Fragments arranged by size:
3965 1123 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2356 2391 529
Size: 35 1792 35 4040
Fragments arranged by size:
4040 1792 35 35
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 2904 3349
Size: 54 1425 50 275 18 270 804 445
Cuts at: 3349 4238 5867 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 804 445 275 270 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2100 3349 1537
Size: 275 288 1249 4090
Fragments arranged by size:
4090 1249 288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2513 3832 1353
Size: 1160 1319 3423
Fragments arranged by size:
3423 1319 1160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4489 4863 5009 387
Size: 1064 138 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2285 2494 2896 3965 4562 5349
Size: 393 524 129 209 402 1069 597 787
Cuts at: 5349 5617 1239
Size: 268 1524
Fragments arranged by size:
1524 1069 787 597 524 402 393 268
209 129
EcoNI CCTnn'n_nnAGG
Cuts at: 3050 3050
Size: 5902
EcoO109I rG'GnC_Cy
Cuts at: 2079 2079
Size: 5902
EcoRI G'AATT_C
Cuts at: 2400 3008 3211 3589 2400
Size: 608 203 378 4713
Fragments arranged by size:
4713 608 378 203
EcoRV GAT'ATC
Cuts at: 2692 2692
Size: 5902
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 2090 5049 1678
Size: 412 2959 2531
Fragments arranged by size:
2959 2531 412
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2844 2849 2876 2881 4543
Size: 32 135 32 1272 5 27 5 1662
Cuts at: 4543 4575 4617 4649 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1662 1272 135 42 32 32 32
32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2353 2409 3037 3246 3492 669
Size: 988 281 415 56 628 209 246 3079
Fragments arranged by size:
3079 988 628 415 281 246 209 56
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KasI G'GCGC_C
Cuts at: 2086 2086
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2507 5016 5200 1305
Size: 1202 2509 184 2007
Fragments arranged by size:
2509 2007 1202 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2906 10
Size: 54 1425 343 1074 3006
Fragments arranged by size:
3006 1425 1074 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3860 4219 4378 518
Size: 648 2694 359 159 2042
Fragments arranged by size:
2694 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2052 2058 2986 3318 3995 5143 5388 1606
Size: 446 6 928 332 677 1148 245 2120
Fragments arranged by size:
2120 1148 928 677 446 332 245 6
NarI GG'CG_CC
Cuts at: 2087 2087
Size: 5902
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4411
Fragments arranged by size:
4411 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 2116 3141 3260 4805 1132
Size: 97 887 1025 119 1545 2229
Fragments arranged by size:
2229 1545 1025 887 119 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5902
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5902
PfoI T'CCnGG_A
Cuts at: 3156 3156
Size: 5902
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2079 2079
Size: 5902
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PstI C_TGCA'G
Cuts at: 830 2302 3254 830
Size: 1472 952 3478
Fragments arranged by size:
3478 1472 952
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
PvuII CAG'CTG
Cuts at: 2986 2986
Size: 5902
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SanDI GG'GwC_CC
Cuts at: 2079 2079
Size: 5902
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2169 2199 2298 2800 3250 4385 5066
Size: 219 1124 30 99 502 450 1135 681
Cuts at: 5066 5257 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1124 681 502 450 219 191
99 30
SfoI GGC'GCC
Cuts at: 2088 2088
Size: 5902
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4006 4907 5169 820
Size: 197 926 2063 901 262 1553
Fragments arranged by size:
2063 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 2116 3141 3260 1132
Size: 984 1025 119 3774
Fragments arranged by size:
3774 1025 984 119
SspI AAT'ATT
Cuts at: 5 52 2707 2731 3826 5
Size: 47 2655 24 1095 2081
Fragments arranged by size:
2655 2081 1095 47 24
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5902
StyI C'CwwG_G
Cuts at: 513 1065 2004 2317 3341 513
Size: 552 939 313 1024 3074
Fragments arranged by size:
3074 1024 939 552 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2394 3929 3946 4099 4284 4729 325
Size: 1255 814 1535 17 153 185 445 1498
Fragments arranged by size:
1535 1498 1255 814 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4148 5860 96
Size: 274 80 33 51 1443 2171 1712 138
Fragments arranged by size:
2171 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2751 2783 5862 5894 2751
Size: 32 3079 32 2759
Fragments arranged by size:
3079 2759 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2372 2710 2372
Size: 338 5564
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 4031 4031
Size: 5902
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SrfI SwaI