Restriction map for
pFN21AB6560
(Circular) (Six-base) MAPSORT of: pFN21AB6560.seq Check: 1061 from: 1 to: 5731
pFN21AB6560 5731 bp
With 182 enzymes: SgfI *
July 29, 2009 14:18 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3074 1937
Size: 1137 4594
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3794 5178 5446 1632
Size: 2162 1384 268 1917
Fragments arranged by size:
2162 1917 1384 268
AfeI AGC'GCT
Cuts at: 2119 2119
Size: 5731
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 2074 2140 4630 5607 1203
Size: 871 66 2490 977 1327
Fragments arranged by size:
2490 1327 977 871 66
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 2079 1166
Size: 913 4818
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2490 2522 2490
Size: 32 5699
AlwNI CAG_nnn'CTG
Cuts at: 3023 3474 5046 3023
Size: 451 1572 3708
Fragments arranged by size:
3708 1572 451
ApaI G_GGCC'C
Cuts at: 2405 2405
Size: 5731
ApaLI G'TGCA_C
Cuts at: 3788 4944 3788
Size: 1156 4575
ApoI r'AATT_y
Cuts at: 1493 3040 3266 3418 5527 1493
Size: 1547 226 152 2109 1697
Fragments arranged by size:
2109 1697 1547 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 3056 1870
Size: 73 1113 4545
Fragments arranged by size:
4545 1113 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2868 2901 2868
Size: 33 5698
BamHI G'GATC_C
Cuts at: 3061 3428 3061
Size: 367 5364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2125 2332 3052 4507 618
Size: 325 217 627 338 207 720 1455 1842
Fragments arranged by size:
1842 1455 720 627 338 325 217 207
BanII G_rGCy'C
Cuts at: 1391 2223 2405 2456 2913 3050 1391
Size: 832 182 51 457 137 4072
Fragments arranged by size:
4072 832 457 182 137 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4760
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3922 3956 1903
Size: 34 1985 34 3678
Fragments arranged by size:
3678 1985 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3623 4833 3623
Size: 1210 4521
BclI T'GATC_A
Cuts at: 1548 2352 1548
Size: 804 4927
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4342 136
Size: 107 122 71 631 605 2670 1525
Fragments arranged by size:
2670 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 2907 3148 2907
Size: 241 5490
Bme1580I G_kGCm'C
Cuts at: 1790 2074 2405 2520 3792 4948 1790
Size: 284 331 115 1272 1156 2573
Fragments arranged by size:
2573 1272 1156 331 284 115
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3462 4420 442
Size: 416 53 83 399 24 2045 958 1753
Fragments arranged by size:
2045 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4391 1239
Size: 3152 2579
Bpu10I CC'TnA_GC
Cuts at: 2217 2217
Size: 5731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2115 3856 4721 5019 2115
Size: 1741 865 298 2827
Fragments arranged by size:
2827 1741 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2570 2857 4394 882
Size: 617 1071 287 1537 2219
Fragments arranged by size:
2219 1537 1071 617 287
BsaAI yAC'GTr
Cuts at: 493 1204 2077 2143 3522 493
Size: 711 873 66 1379 2702
Fragments arranged by size:
2702 1379 873 711 66
BsaBI GATnn'nnATC
Cuts at: 5725 5725
Size: 5731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3538 3920 4555 275
Size: 53 83 186 936 2005 382 635 1451
Fragments arranged by size:
2005 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4164 4562 4836 4983 5394 4164
Size: 398 274 147 411 4501
Fragments arranged by size:
4501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2127 2157 2297 2327 2412
Size: 30 165 30 724 30 140 30 85
Cuts at: 2412 2442 2490 2520 1178
Size: 30 48 30 4389
Fragments arranged by size:
4389 724 165 140 85 48 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2869 1186
Size: 1683 4048
BseYI C'CCAG_C
Cuts at: 2114 2269 2336 2519 2994 4934 5430 2114
Size: 155 67 183 475 1940 496 2415
Fragments arranged by size:
2415 1940 496 475 183 155 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5158
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2399 3181 3942 4970 1540
Size: 275 186 398 782 761 1028 2301
Fragments arranged by size:
2301 1028 782 761 398 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 2456 2940 3792 3877 4948 2223
Size: 233 484 852 85 1071 3006
Fragments arranged by size:
3006 1071 852 484 233 85
BsmI GAATG_Cn'
Cuts at: 3242 3335 3242
Size: 93 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2223 2405 2456 2520 2913 2940
Size: 399 284 149 182 51 64 393 27
Cuts at: 2940 3050 3792 3877 4948 1391
Size: 110 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 393 284 182 149
110 85 64 51 27
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3086 844
Size: 2242 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4226 4400 65
Size: 1183 2978 174 1396
Fragments arranged by size:
2978 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4375 4562 1700
Size: 110 2565 187 2869
Fragments arranged by size:
2869 2565 187 110
BsrGI T'GTAC_A
Cuts at: 96 2190 96
Size: 2094 3637
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 3791 4803 3791
Size: 1012 4719
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5731
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5731
BstXI CCAn_nnnn'nTGG
Cuts at: 2122 2122
Size: 5731
BstYI r'GATC_y
Cuts at: 1972 2606 2960 3061 3428 3813 3830 5282 5368
Size: 634 354 101 367 385 17 1452 86
Cuts at: 5368 5380 5726 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 634 385 367 354 346 101
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2784 2784
Size: 5731
BtgI C'CryG_G
Cuts at: 513 1065 2019 2406 2569 2602 513
Size: 552 954 387 163 33 3642
Fragments arranged by size:
3642 954 552 387 163 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4992
Fragments arranged by size:
4992 683 56
BtsI GCAGTG_nn'
Cuts at: 2520 2639 2812 3247 4041 4061 2520
Size: 119 173 435 794 20 4190
Fragments arranged by size:
4190 794 435 173 119 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3035 3035
Size: 5731
DraIII CAC_nnn'GTG
Cuts at: 2325 2325
Size: 5731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2396 3178 4067
Size: 54 1425 50 275 18 566 782 889
Cuts at: 4067 5696 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 782 566 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2396 3178 1537
Size: 275 584 782 4090
Fragments arranged by size:
4090 782 584 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 3661 1353
Size: 801 1507 3423
Fragments arranged by size:
3423 1507 801
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4318 4692 4838 387
Size: 1064 138 2729 374 146 1280
Fragments arranged by size:
2729 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2221 2454 2221
Size: 233 5498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3794 4391 5178 5446 1239
Size: 393 2162 597 787 268 1524
Fragments arranged by size:
2162 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2133 2133
Size: 5731
EcoO109I rG'GnC_Cy
Cuts at: 2206 2283 2402 2558 2206
Size: 77 119 156 5379
Fragments arranged by size:
5379 156 119 77
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 2121 4878 1678
Size: 443 2757 2531
Fragments arranged by size:
2757 2531 443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4372 4404 4446 4478 1373
Size: 32 135 32 2800 32 42 32 2626
Fragments arranged by size:
2800 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2698 748
Size: 1950 3781
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2787 4845 5029 1305
Size: 1482 2058 184 2007
Fragments arranged by size:
2058 2007 1482 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3909
Fragments arranged by size:
3909 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2079 3689 4048 4207 518
Size: 648 843 70 1610 359 159 2042
Fragments arranged by size:
2042 1610 843 648 359 159 70
MspA1I CmG'CkG
Cuts at: 1606 3023 3147 3824 4972 5217 1606
Size: 1417 124 677 1148 245 2120
Fragments arranged by size:
2120 1417 1148 677 245 124
NcoI C'CATG_G
Cuts at: 513 1065 2602 513
Size: 552 1537 3642
Fragments arranged by size:
3642 1537 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2040 3089 4634 1132
Size: 97 811 1049 1545 2229
Fragments arranged by size:
2229 1545 1049 811 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2325 3023 1108
Size: 1217 698 3816
Fragments arranged by size:
3816 1217 698
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PmlI CAC'GTG
Cuts at: 2077 2143 2077
Size: 66 5665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2490 2522 3800 3832 5313 5345 2490
Size: 32 1278 32 1481 32 2876
Fragments arranged by size:
2876 1481 1278 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 2283 2558 2206
Size: 77 275 5379
Fragments arranged by size:
5379 275 77
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PspOMI G'GGCC_C
Cuts at: 2401 2401
Size: 5731
PstI C_TGCA'G
Cuts at: 830 2444 2515 3083 830
Size: 1614 71 568 3478
Fragments arranged by size:
3478 1614 568 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
PvuII CAG'CTG
Cuts at: 3023 3023
Size: 5731
SacI G_AGCT'C
Cuts at: 2223 2456 2223
Size: 233 5498
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SanDI GG'GwC_CC
Cuts at: 2206 2206
Size: 5731
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 2287 2440 2511 2581 3079 4214 4895
Size: 219 1242 153 71 70 498 1135 681
Cuts at: 4895 5086 826
Size: 191 1471
Fragments arranged by size:
1471 1242 1135 681 498 219 191 153
71 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 3058 1872
Size: 1186 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2130 3835 4736 4998 820
Size: 197 926 187 1705 901 262 1553
Fragments arranged by size:
1705 1553 926 901 262 197 187
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 3089 1132
Size: 1957 3774
SspI AAT'ATT
Cuts at: 5 52 2235 3655 5
Size: 47 2183 1420 2081
Fragments arranged by size:
2183 2081 1420 47
StuI AGG'CCT
Cuts at: 1517 2951 1517
Size: 1434 4297
StyI C'CwwG_G
Cuts at: 513 1065 2602 3170 513
Size: 552 1537 568 3074
Fragments arranged by size:
3074 1537 568 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2032 2069 2350 2582 2701 3758 3775
Size: 1255 452 37 281 232 119 1057 17
Cuts at: 3775 3928 4113 4558 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1057 452 445 281 232 185
153 119 37 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2190 3977 5689
Size: 274 80 33 51 1443 213 1787 1712
Cuts at: 5689 96
Size: 138
Fragments arranged by size:
1787 1712 1443 274 213 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 2297 2329 2412 2444 5691 5723 2127
Size: 32 138 32 83 32 3247 32 2135
Fragments arranged by size:
3247 2135 138 83 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2434 1316
Size: 1118 4613
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2344 2344
Size: 5731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 3056 1870
Size: 1186 4545
XmnI GAAnn'nnTTC
Cuts at: 2246 2665 3860 2246
Size: 419 1195 4117
Fragments arranged by size:
4117 1195 419
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI
RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI