Restriction map for
pFN21AB6555
(Circular) (Six-base) MAPSORT of: pFN21AB6555.seq Check: 289 from: 1 to: 5821
pFN21AB6555 5821 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2589 2589
Size: 5821
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2385 3884 5268 5536 1632
Size: 753 1499 1384 268 1917
Fragments arranged by size:
1917 1499 1384 753 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 2816 1166
Size: 1650 4171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5821
AlwNI CAG_nnn'CTG
Cuts at: 3564 5136 3564
Size: 1572 4249
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 3130 3356 3508 5617 1493
Size: 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2306 2319 4375 160
Size: 872 1274 13 2056 1606
Fragments arranged by size:
2056 1606 1274 872 13
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2213 3146 1870
Size: 73 270 933 4545
Fragments arranged by size:
4545 933 270 73
BamHI G'GATC_C
Cuts at: 2257 3151 3518 2257
Size: 894 367 4560
Fragments arranged by size:
4560 894 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3142 4597 618
Size: 325 217 627 1355 1455 1842
Fragments arranged by size:
1842 1455 1355 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2910 3003 3140 1391
Size: 1519 93 137 4072
Fragments arranged by size:
4072 1519 137 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3051 928
Size: 971 1152 3698
Fragments arranged by size:
3698 1152 971
BbvCI CC'TCA_GC
Cuts at: 2186 2436 2186
Size: 250 5571
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4432 136
Size: 107 122 71 631 605 2760 1525
Fragments arranged by size:
2760 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 3238 3238
Size: 5821
Bme1580I G_kGCm'C
Cuts at: 1790 2931 3882 5038 1790
Size: 1141 951 1156 2573
Fragments arranged by size:
2573 1156 1141 951
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2440 2863 3552
Size: 416 53 83 399 24 1023 423 689
Cuts at: 3552 4510 442
Size: 958 1753
Fragments arranged by size:
1753 1023 958 689 423 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 2432 2507 2663 2867 2933 4481 1239
Size: 1010 183 75 156 204 66 1548 2579
Fragments arranged by size:
2579 1548 1010 204 183 156 75 66
Bpu10I CC'TnA_GC
Cuts at: 2186 2436 2186
Size: 250 5571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3612 493
Size: 711 2408 2702
Fragments arranged by size:
2702 2408 711
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3628 4010 4645 275
Size: 53 83 186 936 2095 382 635 1451
Fragments arranged by size:
2095 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5596
Fragments arranged by size:
5596 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 2246 1186
Size: 985 75 4761
Fragments arranged by size:
4761 985 75
BseYI C'CCAG_C
Cuts at: 5024 5520 5024
Size: 496 5325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2918 1163
Size: 573 1182 4066
Fragments arranged by size:
4066 1182 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3003 3882 3967 5038 3003
Size: 879 85 1071 3786
Fragments arranged by size:
3786 1071 879 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2910 2931 3003 3140 3882 3967 5038
Size: 399 1120 21 72 137 742 85 1071
Cuts at: 5038 1391
Size: 2174
Fragments arranged by size:
2174 1120 1071 742 399 137 85 72
21
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2187 2589 3176 844
Size: 1343 402 587 3489
Fragments arranged by size:
3489 1343 587 402
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4316 4490 65
Size: 1183 3068 174 1396
Fragments arranged by size:
3068 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4465 4652 1700
Size: 110 2655 187 2869
Fragments arranged by size:
2869 2655 187 110
BsrGI T'GTAC_A
Cuts at: 96 3087 96
Size: 2991 2830
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2473 794
Size: 1679 4142
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 2440 1184
Size: 1256 4565
BstXI CCAn_nnnn'nTGG
Cuts at: 2563 2647 2563
Size: 84 5737
BstYI r'GATC_y
Cuts at: 1972 2257 3151 3518 3903 3920 5372 5458 5470
Size: 285 894 367 385 17 1452 86 12
Cuts at: 5470 5816 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 894 385 367 346 285 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3035 513
Size: 552 939 1031 3299
Fragments arranged by size:
3299 1031 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 2278 3337 4131 4151 2278
Size: 1059 794 20 3948
Fragments arranged by size:
3948 1059 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DraIII CAC_nnn'GTG
Cuts at: 2273 2273
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2568 3038
Size: 54 1425 50 275 18 177 561 470
Cuts at: 3038 3268 4157 5786 8
Size: 230 889 1629 43
Fragments arranged by size:
1629 1425 889 561 470 275 230 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3101 3751 1353
Size: 1748 650 3423
Fragments arranged by size:
3423 1748 650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3001 3001
Size: 5821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2385 2432 2507 2663 2867 2933
Size: 393 617 136 47 75 156 204 66
Cuts at: 2933 3884 4481 5268 5536 1239
Size: 951 597 787 268 1524
Fragments arranged by size:
1524 951 787 617 597 393 268 204
156 136 75 66 47
EcoO109I rG'GnC_Cy
Cuts at: 2109 2109
Size: 5821
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 4968 1678
Size: 3290 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 2328 2360 2658
Size: 32 135 32 677 32 47 32 298
Cuts at: 2658 2690 3053 3085 4462 4494 4536 4568 1373
Size: 32 363 32 1377 32 42 32 2626
Fragments arranged by size:
2626 1377 677 363 298 135 47 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3055 3165 3411 669
Size: 988 281 1117 110 246 3079
Fragments arranged by size:
3079 1117 988 281 246 110
HindIII A'AGCT_T
Cuts at: 748 2282 2396 748
Size: 1534 114 4173
Fragments arranged by size:
4173 1534 114
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2314 3000 4935 5119 1305
Size: 1009 686 1935 184 2007
Fragments arranged by size:
2007 1935 1009 686 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2570 10
Size: 54 1425 343 177 561 3261
Fragments arranged by size:
3261 1425 561 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2645 2742 2816 3779 4138 4297 518
Size: 648 1479 97 74 963 359 159 2042
Fragments arranged by size:
2042 1479 963 648 359 159 97 74
MspA1I CmG'CkG
Cuts at: 1606 3037 3237 3914 5062 5307 1606
Size: 1431 200 677 1148 245 2120
Fragments arranged by size:
2120 1431 1148 677 245 200
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4330
Fragments arranged by size:
4330 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 3179 4724 1132
Size: 97 1950 1545 2229
Fragments arranged by size:
2229 1950 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5821
PfoI T'CCnGG_A
Cuts at: 2484 2729 2910 2484
Size: 245 181 5395
Fragments arranged by size:
5395 245 181
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2109 2109
Size: 5821
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PstI C_TGCA'G
Cuts at: 830 2154 2394 2478 3173 830
Size: 1324 240 84 695 3478
Fragments arranged by size:
3478 1324 695 240 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
SacI G_AGCT'C
Cuts at: 3003 3003
Size: 5821
SacII CC_GC'GG
Cuts at: 3038 3038
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SanDI GG'GwC_CC
Cuts at: 2109 2109
Size: 5821
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SexAI A'CCwGG_T
Cuts at: 2889 2889
Size: 5821
SfcI C'TryA_G
Cuts at: 826 1045 2150 2390 2474 3169 4304 4985 5176
Size: 219 1105 240 84 695 1135 681 191
Cuts at: 5176 826
Size: 1471
Fragments arranged by size:
1471 1135 1105 695 681 240 219 191
84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3925 4826 5088 820
Size: 197 926 1982 901 262 1553
Fragments arranged by size:
1982 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2004 2133 3260 513
Size: 552 939 129 1127 3074
Fragments arranged by size:
3074 1127 939 552 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2459 3848 3865 4018 4203 4648 325
Size: 1255 879 1389 17 153 185 445 1498
Fragments arranged by size:
1498 1389 1255 879 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3087 4067 5779
Size: 274 80 33 51 1443 1110 980 1712
Cuts at: 5779 96
Size: 138
Fragments arranged by size:
1712 1443 1110 980 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5781 5813 5781
Size: 32 5789
Tth111I GACn'n_nGTC
Cuts at: 1316 3050 1316
Size: 1734 4087
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2563 2876 2563
Size: 313 5508
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 2399 3950 2399
Size: 1551 4270
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SapI
SfiI SfoI SrfI SwaI