Restriction map for
pFN21AB6552
(Circular) (Six-base) MAPSORT of: pFN21AB6552.seq Check: 379 from: 1 to: 5887
pFN21AB6552 5887 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2325 3230 1937
Size: 388 905 4594
Fragments arranged by size:
4594 905 388
Acc65I G'GTAC_C
Cuts at: 3208 3208
Size: 5887
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2950 3085 3950 5334 5602 1632
Size: 1318 135 865 1384 268 1917
Fragments arranged by size:
1917 1384 1318 865 268 135
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 4786 5763 1203
Size: 3583 977 1327
Fragments arranged by size:
3583 1327 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 2219 1166
Size: 1053 4834
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2156 1184
Size: 972 4915
AlwNI CAG_nnn'CTG
Cuts at: 2263 2395 2559 2775 2986 3007 3630 5202 2263
Size: 132 164 216 211 21 623 1572 2948
Fragments arranged by size:
2948 1572 623 216 211 164 132 21
ApaLI G'TGCA_C
Cuts at: 2837 3125 3944 5100 2837
Size: 288 819 1156 3624
Fragments arranged by size:
3624 1156 819 288
ApoI r'AATT_y
Cuts at: 1493 2471 3196 3422 3574 5683 1493
Size: 978 725 226 152 2109 1697
Fragments arranged by size:
2109 1697 978 725 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 3043 3212 1870
Size: 73 1100 169 4545
Fragments arranged by size:
4545 1100 169 73
AvrII C'CTAG_G
Cuts at: 2188 2188
Size: 5887
BamHI G'GATC_C
Cuts at: 3217 3584 3217
Size: 367 5520
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2108 3208 4663 618
Size: 325 217 627 321 1100 1455 1842
Fragments arranged by size:
1842 1455 1100 627 325 321 217
BanII G_rGCy'C
Cuts at: 1391 2243 2538 3206 1391
Size: 852 295 668 4072
Fragments arranged by size:
4072 852 668 295
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3091 928
Size: 971 1192 3724
Fragments arranged by size:
3724 1192 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4078 4112 1903
Size: 34 2141 34 3678
Fragments arranged by size:
3678 2141 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3779 4989 3779
Size: 1210 4677
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2850 4498 136
Size: 107 122 71 631 605 1178 1648 1525
Fragments arranged by size:
1648 1525 1178 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5882 5882
Size: 5887
BlpI GC'TnA_GC
Cuts at: 3304 3304
Size: 5887
Bme1580I G_kGCm'C
Cuts at: 1790 2841 3129 3948 5104 1790
Size: 1051 288 819 1156 2573
Fragments arranged by size:
2573 1156 1051 819 288
BmgBI CAC'GTC
Cuts at: 1318 3015 1318
Size: 1697 4190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2517 2735 3618
Size: 416 53 83 399 24 1100 218 883
Cuts at: 3618 4576 442
Size: 958 1753
Fragments arranged by size:
1753 1100 958 883 416 399 218 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3039 1056
Size: 1983 3904
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 2933 4547 1239
Size: 1034 660 1614 2579
Fragments arranged by size:
2579 1614 1034 660
Bpu10I CC'TnA_GC
Cuts at: 2235 2235
Size: 5887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4012 4877 5175 4012
Size: 865 298 4724
Fragments arranged by size:
4724 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3118 4550 882
Size: 617 1619 1432 2219
Fragments arranged by size:
2219 1619 1432 617
BsaAI yAC'GTr
Cuts at: 493 1204 3678 493
Size: 711 2474 2702
Fragments arranged by size:
2702 2474 711
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2658 3694 4076 4711
Size: 53 83 186 936 1125 1036 382 635
Cuts at: 4711 275
Size: 1451
Fragments arranged by size:
1451 1125 1036 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3109 4320 4718 4992 5139 5550 3109
Size: 1211 398 274 147 411 3446
Fragments arranged by size:
3446 1211 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2756 2786 3160 3190 1178
Size: 30 165 30 1353 30 374 30 3875
Fragments arranged by size:
3875 1353 374 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2033 2288 2291 2680 2930 1186
Size: 847 255 3 389 250 4143
Fragments arranged by size:
4143 847 389 255 250 3
BseYI C'CCAG_C
Cuts at: 3137 3179 5090 5586 3137
Size: 42 1911 496 3438
Fragments arranged by size:
3438 1911 496 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2408 3140 1163
Size: 573 672 732 3910
Fragments arranged by size:
3910 732 672 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2864 3337 4098 5126 1540
Size: 275 186 863 473 761 1028 2301
Fragments arranged by size:
2301 1028 863 761 473 275 186
BsiHKAI G_wGCw'C
Cuts at: 2049 2841 3129 3948 4033 5104 2049
Size: 792 288 819 85 1071 2832
Fragments arranged by size:
2832 1071 819 792 288 85
BsmI GAATG_Cn'
Cuts at: 3398 3491 3398
Size: 93 5794
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2243 2538 2841 3129 3206 3948
Size: 399 259 194 295 303 288 77 742
Cuts at: 3948 4033 5104 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 303 295 288 259
194 85 77
BspEI T'CCGG_A
Cuts at: 5550 5550
Size: 5887
BspHI T'CATG_A
Cuts at: 3776 5506 3776
Size: 1730 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3162 3242 844
Size: 2318 80 3489
Fragments arranged by size:
3489 2318 80
BsrBI CCG'CTC
Cuts at: 1394 1823 2127 3061 3774 1394
Size: 429 304 934 713 3507
Fragments arranged by size:
3507 934 713 429 304
BsrDI GCAATG_nn'
Cuts at: 65 1248 4382 4556 65
Size: 1183 3134 174 1396
Fragments arranged by size:
3134 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4531 4718 1700
Size: 110 2721 187 2869
Fragments arranged by size:
2869 2721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 2344 1925
Size: 419 5468
BssSI C'ACGA_G
Cuts at: 3947 4959 3947
Size: 1012 4875
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2263 2395 2986 794
Size: 1469 132 591 3695
Fragments arranged by size:
3695 1469 591 132
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5887
BstXI CCAn_nnnn'nTGG
Cuts at: 2494 2878 2494
Size: 384 5503
BstYI r'GATC_y
Cuts at: 1972 2259 2598 3217 3584 3969 3986 5438 5524
Size: 287 339 619 367 385 17 1452 86
Cuts at: 5524 5536 5882 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 619 385 367 346 339 287
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2104 2821 3117 513
Size: 552 939 100 717 296 3283
Fragments arranged by size:
3283 939 717 552 296 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5148
Fragments arranged by size:
5148 683 56
BtsI GCAGTG_nn'
Cuts at: 2047 2629 2839 3403 4197 4217 2047
Size: 582 210 564 794 20 3717
Fragments arranged by size:
3717 794 582 564 210 20
ClaI AT'CG_AT
Cuts at: 1554 2802 1554
Size: 1248 4639
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5852
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4894 809
Size: 507 3578 1802
Fragments arranged by size:
3578 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2865 3009 3334
Size: 54 1425 50 275 18 1035 144 325
Cuts at: 3334 4223 5852 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1035 889 325 275 144 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3334 1537
Size: 275 1522 4090
Fragments arranged by size:
4090 1522 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3817 1353
Size: 2464 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4474 4848 4994 387
Size: 1064 138 2885 374 146 1280
Fragments arranged by size:
2885 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2933 2950 3085 3950 4547 5334
Size: 393 641 660 17 135 865 597 787
Cuts at: 5334 5602 1239
Size: 268 1524
Fragments arranged by size:
1524 865 787 660 641 597 393 268
135 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2360
Size: 5887
EcoO109I rG'GnC_Cy
Cuts at: 2491 2565 2588 3047 2491
Size: 74 23 459 5331
Fragments arranged by size:
5331 459 74 23
EcoRI G'AATT_C
Cuts at: 2471 3196 3574 2471
Size: 725 378 4784
Fragments arranged by size:
4784 725 378
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 2396 2944 5034 1678
Size: 718 548 2090 2531
Fragments arranged by size:
2531 2090 718 548
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2756 2788 4528 4560 4602
Size: 32 135 32 1184 32 1740 32 42
Cuts at: 4602 4634 1373
Size: 32 2626
Fragments arranged by size:
2626 1740 1184 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2200 3231 3477 669
Size: 988 281 262 1031 246 3079
Fragments arranged by size:
3079 1031 988 281 262 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5887
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KpnI G_GTAC'C
Cuts at: 3212 3212
Size: 5887
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5001 5185 1305
Size: 3696 184 2007
Fragments arranged by size:
3696 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3011 10
Size: 54 1425 343 1179 2886
Fragments arranged by size:
2886 1425 1179 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2219 2741 3845 4204 4363 518
Size: 648 1053 522 1104 359 159 2042
Fragments arranged by size:
2042 1104 1053 648 522 359 159
MspA1I CmG'CkG
Cuts at: 1606 2097 2983 3183 3303 3980 5128 5373 1606
Size: 491 886 200 120 677 1148 245 2120
Fragments arranged by size:
2120 1148 886 677 491 245 200 120
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4396
Fragments arranged by size:
4396 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NheI G'CTAG_C
Cuts at: 1052 3035 1052
Size: 1983 3904
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 2653 3245 4790 1132
Size: 97 1424 592 1545 2229
Fragments arranged by size:
2229 1545 1424 592 97
PasI CC'CwG_GG
Cuts at: 1348 2113 2662 1348
Size: 765 549 4573
Fragments arranged by size:
4573 765 549
PciI A'CATG_T
Cuts at: 4786 4786
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3007 1108
Size: 1899 3988
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 5469 5501 3956
Size: 32 1481 32 4342
Fragments arranged by size:
4342 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2565 2588 3047 2565
Size: 23 459 5405
Fragments arranged by size:
5405 459 23
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PstI C_TGCA'G
Cuts at: 830 2042 2156 2555 2991 3239 830
Size: 1212 114 399 436 248 3478
Fragments arranged by size:
3478 1212 436 399 248 114
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
PvuII CAG'CTG
Cuts at: 2983 3183 2983
Size: 200 5687
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SanDI GG'GwC_CC
Cuts at: 2565 3047 2565
Size: 482 5405
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SfcI C'TryA_G
Cuts at: 826 1045 2038 2152 2551 2987 3235 4370 5051
Size: 219 993 114 399 436 248 1135 681
Cuts at: 5051 5242 826
Size: 191 1471
Fragments arranged by size:
1471 1135 993 681 436 399 248 219
191 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3991 4892 5154 820
Size: 197 926 2048 901 262 1553
Fragments arranged by size:
2048 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 3811 5
Size: 47 3759 2081
Fragments arranged by size:
3759 2081 47
StuI AGG'CCT
Cuts at: 1517 2921 1517
Size: 1404 4483
StyI C'CwwG_G
Cuts at: 513 1065 2004 2188 2487 2538 3326 513
Size: 552 939 184 299 51 788 3074
Fragments arranged by size:
3074 939 788 552 299 184 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2185 3914 3931 4084 4269 4714 325
Size: 1255 605 1729 17 153 185 445 1498
Fragments arranged by size:
1729 1498 1255 605 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2727 2796 4133
Size: 274 80 33 51 1443 750 69 1337
Cuts at: 4133 5845 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1337 750 274 138 80 69
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3160 3192 5847 5879 3160
Size: 32 2655 32 3168
Fragments arranged by size:
3168 2655 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5887
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2536 2875 2011
Size: 525 339 5023
Fragments arranged by size:
5023 525 339
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5887
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 4016 4016
Size: 5887
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI
SwaI