Restriction map for
pFN21AB6551
(Circular) (Six-base) MAPSORT of: pFN21AB6551.seq Check: 2578 from: 1 to: 5818
pFN21AB6551 5818 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3628 278
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3161 1937
Size: 1224 4594
Acc65I G'GTAC_C
Cuts at: 3139 3139
Size: 5818
AclI AA'CG_TT
Cuts at: 1563 3945 4318 1563
Size: 2382 373 3063
Fragments arranged by size:
3063 2382 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2460 3881 5265 5533 1632
Size: 828 1421 1384 268 1917
Fragments arranged by size:
1917 1421 1384 828 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5621
AflIII A'CryG_T
Cuts at: 1203 4717 5694 1203
Size: 3514 977 1327
Fragments arranged by size:
3514 1327 977
AgeI A'CCGG_T
Cuts at: 4649 4649
Size: 5818
AhdI GACnn_n'nnGTC
Cuts at: 2084 4547 2084
Size: 2463 3355
AleI CACnn'nnGTG
Cuts at: 1166 2634 1166
Size: 1468 4350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5818
AlwNI CAG_nnn'CTG
Cuts at: 3561 5133 3561
Size: 1572 4246
ApaI G_GGCC'C
Cuts at: 2032 3042 2032
Size: 1010 4808
ApaLI G'TGCA_C
Cuts at: 3875 5031 3875
Size: 1156 4662
ApoI r'AATT_y
Cuts at: 1493 2527 3127 3353 3505 5614 1493
Size: 1034 600 226 152 2109 1697
Fragments arranged by size:
2109 1697 1034 600 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4372 160
Size: 872 3340 1606
Fragments arranged by size:
3340 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 2820 2856 2889 2955 3143 1870
Size: 73 115 762 36 33 66 188 4545
Fragments arranged by size:
4545 762 188 115 73 66 36 33
BamHI G'GATC_C
Cuts at: 3148 3515 3148
Size: 367 5451
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2904 3139 4594 618
Size: 325 217 627 1117 235 1455 1842
Fragments arranged by size:
1842 1455 1117 627 325 235 217
BanII G_rGCy'C
Cuts at: 1391 2032 2313 3042 3065 3137 1391
Size: 641 281 729 23 72 4072
Fragments arranged by size:
4072 729 641 281 72 23
BbeI G_GCGC'C
Cuts at: 2908 2908
Size: 5818
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4847
BbvCI CC'TCA_GC
Cuts at: 2511 2511
Size: 5818
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4009 4043 1903
Size: 34 2072 34 3678
Fragments arranged by size:
3678 2072 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3710 4920 3710
Size: 1210 4608
BclI T'GATC_A
Cuts at: 1548 2680 1548
Size: 1132 4686
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4429 136
Size: 107 122 71 631 605 2757 1525
Fragments arranged by size:
2757 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2846 5813 2846
Size: 2967 2851
BlpI GC'TnA_GC
Cuts at: 3235 3235
Size: 5818
Bme1580I G_kGCm'C
Cuts at: 1790 2032 2054 3042 3879 5035 1790
Size: 242 22 988 837 1156 2573
Fragments arranged by size:
2573 1156 988 837 242 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5818
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3549 4507 442
Size: 416 53 83 399 24 2132 958 1753
Fragments arranged by size:
2132 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2968 3070 4478 1239
Size: 1729 102 1408 2579
Fragments arranged by size:
2579 1729 1408 102
Bpu10I CC'TnA_GC
Cuts at: 2511 2511
Size: 5818
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2755 3077 3943 4808 5106 2755
Size: 322 866 865 298 3467
Fragments arranged by size:
3467 866 865 322 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2232 2603 4481 882
Size: 617 733 371 1878 2219
Fragments arranged by size:
2219 1878 733 617 371
BsaAI yAC'GTr
Cuts at: 493 1204 3609 493
Size: 711 2405 2702
Fragments arranged by size:
2702 2405 711
BsaBI GATnn'nnATC
Cuts at: 2898 5812 2898
Size: 2914 2904
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2830 2905 3625 4007
Size: 53 83 186 936 1297 75 720 382
Cuts at: 4007 4642 275
Size: 635 1451
Fragments arranged by size:
1451 1297 936 720 635 382 186 83
75 53
BsaWI w'CCGG_w
Cuts at: 4251 4649 4923 5070 5481 4251
Size: 398 274 147 411 4588
Fragments arranged by size:
4588 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2966 2996 1178
Size: 30 165 30 1563 30 4000
Fragments arranged by size:
4000 1563 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2752 2755 2762 2777 3020 1186
Size: 1566 3 7 15 243 3984
Fragments arranged by size:
3984 1566 243 15 7 3
BseYI C'CCAG_C
Cuts at: 2153 5021 5517 2153
Size: 2868 496 2454
Fragments arranged by size:
2868 2454 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5245
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3268 4029 5057 1540
Size: 275 186 1267 761 1028 2301
Fragments arranged by size:
2301 1267 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 3065 3879 3964 5035 2313
Size: 752 814 85 1071 3096
Fragments arranged by size:
3096 1071 814 752 85
BsmI GAATG_Cn'
Cuts at: 3329 3422 3329
Size: 93 5725
BsmBI CGTCTCn'nnnn_
Cuts at: 2061 2061
Size: 5818
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2054 2313 3042 3065 3137 3879
Size: 399 242 22 259 729 23 72 742
Cuts at: 3879 3964 5035 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 729 399 259 242 85
72 23 22
BspEI T'CCGG_A
Cuts at: 5481 5481
Size: 5818
BspHI T'CATG_A
Cuts at: 3707 5437 3707
Size: 1730 4088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3173 844
Size: 2329 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2992 3021 3705 1394
Size: 429 1169 29 684 3507
Fragments arranged by size:
3507 1169 684 429 29
BsrDI GCAATG_nn'
Cuts at: 65 1248 2268 4313 4487 65
Size: 1183 1020 2045 174 1396
Fragments arranged by size:
2045 1396 1183 1020 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3107 4462 4649 1700
Size: 110 1297 1355 187 2869
Fragments arranged by size:
2869 1355 1297 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5818
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5818
BssSI C'ACGA_G
Cuts at: 3878 4890 3878
Size: 1012 4806
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2563 794
Size: 1769 4049
BstBI TT'CG_AA
Cuts at: 4622 4622
Size: 5818
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5818
BstXI CCAn_nnnn'nTGG
Cuts at: 2636 2636
Size: 5818
BstYI r'GATC_y
Cuts at: 1972 2664 2846 3148 3515 3900 3917 5369 5455
Size: 692 182 302 367 385 17 1452 86
Cuts at: 5455 5467 5813 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 692 385 367 346 302 182
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5266
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5079
Fragments arranged by size:
5079 683 56
BtsI GCAGTG_nn'
Cuts at: 3334 4128 4148 3334
Size: 794 20 5004
Fragments arranged by size:
5004 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5818
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5783
DraI TTT'AAA
Cuts at: 3122 3122
Size: 5818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4825 809
Size: 507 3509 1802
Fragments arranged by size:
3509 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3265 4154 5783
Size: 54 1425 50 275 18 1435 889 1629
Cuts at: 5783 8
Size: 43
Fragments arranged by size:
1629 1435 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3265 1537
Size: 275 1453 4090
Fragments arranged by size:
4090 1453 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2711 3090 3748 1353
Size: 1358 379 658 3423
Fragments arranged by size:
3423 1358 658 379
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 1999 3040 4405 4779 4925 387
Size: 1064 138 410 1041 1365 374 146 1280
Fragments arranged by size:
1365 1280 1064 1041 410 374 146 138
EcoICRI GAG'CTC
Cuts at: 2311 3063 2311
Size: 752 5066
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2460 2968 3070 3881 4478 5265 5533
Size: 393 828 508 102 811 597 787 268
Cuts at: 5533 1239
Size: 1524
Fragments arranged by size:
1524 828 811 787 597 508 393 268
102
EcoO109I rG'GnC_Cy
Cuts at: 2029 3038 3039 2029
Size: 1009 1 4808
Fragments arranged by size:
4808 1009 1
EcoRI G'AATT_C
Cuts at: 3127 3505 3127
Size: 378 5440
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 5818
FspI TGC'GCA
Cuts at: 4324 4324
Size: 5818
HaeII r_GCGC'y
Cuts at: 1678 2908 4965 1678
Size: 1230 2057 2531
Fragments arranged by size:
2531 2057 1230
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2070 2102 2981 3013 4459
Size: 32 135 32 498 32 879 32 1446
Cuts at: 4459 4491 4533 4565 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1446 879 498 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3162 3408 669
Size: 988 281 1224 246 3079
Fragments arranged by size:
3079 1224 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2675 748
Size: 1927 3891
HpaI GTT'AAC
Cuts at: 3408 3408
Size: 5818
KasI G'GCGC_C
Cuts at: 2904 2904
Size: 5818
KpnI G_GTAC'C
Cuts at: 3143 3143
Size: 5818
MfeI C'AATT_G
Cuts at: 3417 3417
Size: 5818
MluI A'CGCG_T
Cuts at: 5694 5694
Size: 5818
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2813 3090 4932 5116 1305
Size: 1508 277 1842 184 2007
Fragments arranged by size:
2007 1842 1508 277 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3996
Fragments arranged by size:
3996 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2607 2634 3776 4135 4294 518
Size: 648 1441 27 1142 359 159 2042
Fragments arranged by size:
2042 1441 1142 648 359 159 27
MspA1I CmG'CkG
Cuts at: 1606 3234 3911 5059 5304 1606
Size: 1628 677 1148 245 2120
Fragments arranged by size:
2120 1628 1148 677 245
NaeI GCC'GGC
Cuts at: 3109 3109
Size: 5818
NarI GG'CG_CC
Cuts at: 2905 2905
Size: 5818
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5266
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5818
NgoMIV G'CCGG_C
Cuts at: 3107 3107
Size: 5818
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5818
NotI GC'GGCC_GC
Cuts at: 3265 3265
Size: 5818
NspI r_CATG'y
Cuts at: 1132 1229 3176 4721 1132
Size: 97 1947 1545 2229
Fragments arranged by size:
2229 1947 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5818
PciI A'CATG_T
Cuts at: 4717 4717
Size: 5818
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 2197 1108
Size: 939 150 4729
Fragments arranged by size:
4729 939 150
PfoI T'CCnGG_A
Cuts at: 2921 2921
Size: 5818
PmeI GTTT'AAAC
Cuts at: 3122 3122
Size: 5818
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3887 3919 5400 5432 3887
Size: 32 1481 32 4273
Fragments arranged by size:
4273 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5818
PsiI TTA'TAA
Cuts at: 3388 3388
Size: 5818
PspOMI G'GGCC_C
Cuts at: 2028 3038 2028
Size: 1010 4808
PstI C_TGCA'G
Cuts at: 830 3170 830
Size: 2340 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5818
SacI G_AGCT'C
Cuts at: 2313 3065 2313
Size: 752 5066
SalI G'TCGA_C
Cuts at: 1936 3160 1936
Size: 1224 4594
SbfI CC_TGCA'GG
Cuts at: 3170 3170
Size: 5818
ScaI AGT'ACT
Cuts at: 4066 5778 4066
Size: 1712 4106
SfcI C'TryA_G
Cuts at: 826 1045 3166 4301 4982 5173 826
Size: 219 2121 1135 681 191 1471
Fragments arranged by size:
2121 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2906 2906
Size: 5818
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5818
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5818
SmaI CCC'GGG
Cuts at: 1872 2822 2957 3145 1872
Size: 950 135 188 4545
Fragments arranged by size:
4545 950 188 135
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2734 3056 3922 4823 5085 820
Size: 197 926 791 322 866 901 262 1553
Fragments arranged by size:
1553 926 901 866 791 322 262 197
SnaBI TAC'GTA
Cuts at: 493 3609 493
Size: 3116 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5818
SphI G_CATG'C
Cuts at: 1132 3176 1132
Size: 2044 3774
SspI AAT'ATT
Cuts at: 5 52 3742 5
Size: 47 3690 2081
Fragments arranged by size:
3690 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5818
StyI C'CwwG_G
Cuts at: 513 1065 2593 3257 513
Size: 552 1528 664 3074
Fragments arranged by size:
3074 1528 664 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2976 3845 3862 4015 4200 4645 325
Size: 1255 1396 869 17 153 185 445 1498
Fragments arranged by size:
1498 1396 1255 869 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2380 4064 5776
Size: 274 80 33 51 1443 403 1684 1712
Cuts at: 5776 96
Size: 138
Fragments arranged by size:
1712 1684 1443 403 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2261 2293 5778 5810 2261
Size: 32 3485 32 2269
Fragments arranged by size:
3485 2269 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2105 2943 1316
Size: 789 838 4191
Fragments arranged by size:
4191 838 789
XbaI T'CTAG_A
Cuts at: 3154 3154
Size: 5818
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 3073 2323
Size: 750 5068
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5818
XmaI C'CCGG_G
Cuts at: 1870 2820 2955 3143 1870
Size: 950 135 188 4545
Fragments arranged by size:
4545 950 188 135
XmnI GAAnn'nnTTC
Cuts at: 2487 3947 2487
Size: 1460 4358
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3626 276
Size: 53 83 186 936 2092 2468
Fragments arranged by size:
2468 2092 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI