Restriction map for
pFN21AB6550
(Circular) (Six-base) MAPSORT of: pFN21AB6550.seq Check: 4230 from: 1 to: 5881
pFN21AB6550 5881 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3224 1937
Size: 1287 4594
Acc65I G'GTAC_C
Cuts at: 3202 3202
Size: 5881
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2376 2460 3177 3944 5328 5596 1632
Size: 744 84 717 767 1384 268 1917
Fragments arranged by size:
1917 1384 767 744 717 268 84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5684
AflIII A'CryG_T
Cuts at: 1203 2377 4780 5757 1203
Size: 1174 2403 977 1327
Fragments arranged by size:
2403 1327 1174 977
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AlwNI CAG_nnn'CTG
Cuts at: 3624 5196 3624
Size: 1572 4309
ApaLI G'TGCA_C
Cuts at: 3938 5094 3938
Size: 1156 4725
ApoI r'AATT_y
Cuts at: 1493 2090 2527 3190 3416 3568 5677 1493
Size: 597 437 663 226 152 2109 1697
Fragments arranged by size:
2109 1697 663 597 437 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 2892 3206 1870
Size: 73 115 834 314 4545
Fragments arranged by size:
4545 834 314 115 73
BamHI G'GATC_C
Cuts at: 3211 3578 3211
Size: 367 5514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 3202 4657 618
Size: 325 217 627 264 1151 1455 1842
Fragments arranged by size:
1842 1455 1151 627 325 264 217
BanII G_rGCy'C
Cuts at: 1391 3155 3200 1391
Size: 1764 45 4072
Fragments arranged by size:
4072 1764 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3147 928
Size: 971 1248 3662
Fragments arranged by size:
3662 1248 971
BbvCI CC'TCA_GC
Cuts at: 2777 2777
Size: 5881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2546 2580 4072 4106 1903
Size: 34 609 34 1492 34 3678
Fragments arranged by size:
3678 1492 609 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3773 4983 3773
Size: 1210 4671
BclI T'GATC_A
Cuts at: 1548 2626 1548
Size: 1078 4803
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4492 136
Size: 107 122 71 631 605 337 2483 1525
Fragments arranged by size:
2483 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5876 5876
Size: 5881
BlpI GC'TnA_GC
Cuts at: 3298 3298
Size: 5881
Bme1580I G_kGCm'C
Cuts at: 1790 2054 3942 5098 1790
Size: 264 1888 1156 2573
Fragments arranged by size:
2573 1888 1156 264
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3612 4570 442
Size: 416 53 83 399 24 2195 958 1753
Fragments arranged by size:
2195 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2895 2895
Size: 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2908 4541 1239
Size: 1669 1633 2579
Fragments arranged by size:
2579 1669 1633
Bpu10I CC'TnA_GC
Cuts at: 2777 2777
Size: 5881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2322 4006 4871 5169 2322
Size: 1684 865 298 3034
Fragments arranged by size:
3034 1684 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3021 4544 882
Size: 617 1522 1523 2219
Fragments arranged by size:
2219 1523 1522 617
BsaAI yAC'GTr
Cuts at: 493 1204 2861 3672 493
Size: 711 1657 811 2702
Fragments arranged by size:
2702 1657 811 711
BsaBI GATnn'nnATC
Cuts at: 2295 2887 5875 2295
Size: 592 2988 2301
Fragments arranged by size:
2988 2301 592
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3688 4070 4705 275
Size: 53 83 186 936 2155 382 635 1451
Fragments arranged by size:
2155 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4314 4712 4986 5133 5544 4314
Size: 398 274 147 411 4651
Fragments arranged by size:
4651 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5656
Fragments arranged by size:
5656 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2794 2887 3016 3163 1186
Size: 1608 93 129 147 3904
Fragments arranged by size:
3904 1608 147 129 93
BseYI C'CCAG_C
Cuts at: 5084 5580 5084
Size: 496 5385
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2630 1163
Size: 573 894 4414
Fragments arranged by size:
4414 894 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3331 4092 5120 1540
Size: 275 186 1330 761 1028 2301
Fragments arranged by size:
2301 1330 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3155 3942 4027 5098 3155
Size: 787 85 1071 3938
Fragments arranged by size:
3938 1071 787 85
BsmI GAATG_Cn'
Cuts at: 3392 3485 3392
Size: 93 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 3155 3200 3942 4027 5098 1391
Size: 399 264 1101 45 742 85 1071 2174
Fragments arranged by size:
2174 1101 1071 742 399 264 85 45
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 2140 2383 2602 3770 5500 2140
Size: 243 219 1168 1730 2521
Fragments arranged by size:
2521 1730 1168 243 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3236 844
Size: 2392 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2210 2922 3768 1394
Size: 429 387 712 846 3507
Fragments arranged by size:
3507 846 712 429 387
BsrDI GCAATG_nn'
Cuts at: 65 1248 2283 4376 4550 65
Size: 1183 1035 2093 174 1396
Fragments arranged by size:
2093 1396 1183 1035 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4525 4712 1700
Size: 110 2715 187 2869
Fragments arranged by size:
2869 2715 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5881
BssHII G'CGCG_C
Cuts at: 1925 2026 1925
Size: 101 5780
BssSI C'ACGA_G
Cuts at: 3081 3941 4953 3081
Size: 860 1012 4009
Fragments arranged by size:
4009 1012 860
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5881
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 2167 2813 3005 3211 3578 3963 3980 5432
Size: 195 646 192 206 367 385 17 1452
Cuts at: 5432 5518 5530 5876 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 646 385 367 346 206 195
192 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2262 513
Size: 552 1197 4132
Fragments arranged by size:
4132 1197 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2045 2816 2891 487
Size: 683 56 819 771 75 3477
Fragments arranged by size:
3477 819 771 683 75 56
BtsI GCAGTG_nn'
Cuts at: 3397 4191 4211 3397
Size: 794 20 5067
Fragments arranged by size:
5067 794 20
ClaI AT'CG_AT
Cuts at: 1554 2842 1554
Size: 1288 4593
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5846
DraI TTT'AAA
Cuts at: 3185 3185
Size: 5881
DraIII CAC_nnn'GTG
Cuts at: 3083 3083
Size: 5881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2313 3328 4217
Size: 54 1425 50 275 18 483 1015 889
Cuts at: 4217 5846 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1015 889 483 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3328 1537
Size: 275 1516 4090
Fragments arranged by size:
4090 1516 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2337 2737 2742 3153 3811 1353
Size: 984 400 5 411 658 3423
Fragments arranged by size:
3423 984 658 411 400 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4468 4842 4988 387
Size: 1064 138 2879 374 146 1280
Fragments arranged by size:
2879 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3153 3153
Size: 5881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2376 2460 2908 3177 3944 4541 5328
Size: 393 744 84 448 269 767 597 787
Cuts at: 5328 5596 1239
Size: 268 1524
Fragments arranged by size:
1524 787 767 744 597 448 393 269
268 84
EcoO109I rG'GnC_Cy
Cuts at: 2784 2916 2784
Size: 132 5749
EcoRI G'AATT_C
Cuts at: 2527 3190 3568 2527
Size: 663 378 4840
Fragments arranged by size:
4840 663 378
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 5028 1678
Size: 3350 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3026 3058 4522 4554 4596
Size: 32 135 32 1454 32 1464 32 42
Cuts at: 4596 4628 1373
Size: 32 2626
Fragments arranged by size:
2626 1464 1454 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2081 3225 3471 669
Size: 988 281 143 1144 246 3079
Fragments arranged by size:
3079 1144 988 281 246 143
HindIII A'AGCT_T
Cuts at: 748 2675 748
Size: 1927 3954
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 3206 3206
Size: 5881
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4995 5179 1305
Size: 3690 184 2007
Fragments arranged by size:
3690 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2315 10
Size: 54 1425 343 483 3576
Fragments arranged by size:
3576 1425 483 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2607 3839 4198 4357 518
Size: 648 1441 1232 359 159 2042
Fragments arranged by size:
2042 1441 1232 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3297 3974 5122 5367 1606
Size: 1691 677 1148 245 2120
Fragments arranged by size:
2120 1691 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2262 513
Size: 552 1197 4132
Fragments arranged by size:
4132 1197 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 3239 4784 1132
Size: 97 2010 1545 2229
Fragments arranged by size:
2229 2010 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5881
PciI A'CATG_T
Cuts at: 4780 4780
Size: 5881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 2197 1108
Size: 939 150 4792
Fragments arranged by size:
4792 939 150
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PmlI CAC'GTG
Cuts at: 2861 2861
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 5463 5495 3950
Size: 32 1481 32 4336
Fragments arranged by size:
4336 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2784 2916 2784
Size: 132 5749
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5881
PsiI TTA'TAA
Cuts at: 3451 3451
Size: 5881
PstI C_TGCA'G
Cuts at: 830 2277 3233 830
Size: 1447 956 3478
Fragments arranged by size:
3478 1447 956
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
SacI G_AGCT'C
Cuts at: 3155 3155
Size: 5881
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SapI GCTCTTCn'nnn_
Cuts at: 3153 3153
Size: 5881
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SfcI C'TryA_G
Cuts at: 826 1045 2273 3229 4364 5045 5236 826
Size: 219 1228 956 1135 681 191 1471
Fragments arranged by size:
1471 1228 1135 956 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 2060 3208 1872
Size: 188 1148 4545
Fragments arranged by size:
4545 1148 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2337 3985 4886 5148 820
Size: 197 926 394 1648 901 262 1553
Fragments arranged by size:
1648 1553 926 901 394 262 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5881
StyI C'CwwG_G
Cuts at: 513 1065 2262 2418 3320 513
Size: 552 1197 156 902 3074
Fragments arranged by size:
3074 1197 902 552 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3908 3925 4078 4263 4708 325
Size: 1255 2328 17 153 185 445 1498
Fragments arranged by size:
2328 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4127 5839 96
Size: 274 80 33 51 1443 2150 1712 138
Fragments arranged by size:
2150 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5841 5873 5841
Size: 32 5849
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5881
XbaI T'CTAG_A
Cuts at: 2972 3217 2972
Size: 245 5636
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5881
XmaI C'CCGG_G
Cuts at: 1870 2058 3206 1870
Size: 188 1148 4545
Fragments arranged by size:
4545 1148 188
XmnI GAAnn'nnTTC
Cuts at: 2379 4010 2379
Size: 1631 4250
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI