Restriction map for
pFN21AB6549
(Circular) (Six-base) MAPSORT of: pFN21AB6549.seq Check: 4671 from: 1 to: 5815
pFN21AB6549 5815 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2233 2233
Size: 5815
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2280 3625 278
Size: 53 83 186 936 744 1345 2468
Fragments arranged by size:
2468 1345 936 744 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 1937
Size: 1221 4594
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2740 2905 2944 3878 5262 5530 1632
Size: 1108 165 39 934 1384 268 1917
Fragments arranged by size:
1917 1384 1108 934 268 165 39
AflII C'TTAA_G
Cuts at: 820 1017 2665 820
Size: 197 1648 3970
Fragments arranged by size:
3970 1648 197
AflIII A'CryG_T
Cuts at: 1203 2631 4714 5691 1203
Size: 1428 2083 977 1327
Fragments arranged by size:
2083 1428 1327 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 2297 2726 2864 1166
Size: 1131 429 138 4117
Fragments arranged by size:
4117 1131 429 138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5815
AlwNI CAG_nnn'CTG
Cuts at: 2248 2404 3558 5130 2248
Size: 156 1154 1572 2933
Fragments arranged by size:
2933 1572 1154 156
ApaLI G'TGCA_C
Cuts at: 3011 3872 5028 3011
Size: 861 1156 3798
Fragments arranged by size:
3798 1156 861
ApoI r'AATT_y
Cuts at: 1493 2552 2579 2750 3124 3350 3502 5611 1493
Size: 1059 27 171 374 226 152 2109 1697
Fragments arranged by size:
2109 1697 1059 374 226 171 152 27
AseI AT'TA_AT
Cuts at: 160 1032 4369 160
Size: 872 3337 1606
Fragments arranged by size:
3337 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 3140 1870
Size: 73 1197 4545
Fragments arranged by size:
4545 1197 73
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3136 4591 618
Size: 325 217 627 1349 1455 1842
Fragments arranged by size:
1842 1455 1349 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2337 2613 3134 1391
Size: 946 276 521 4072
Fragments arranged by size:
4072 946 521 276
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4006 4040 1903
Size: 34 2069 34 3678
Fragments arranged by size:
3678 2069 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4426 136
Size: 107 122 71 631 605 2754 1525
Fragments arranged by size:
2754 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5810 5810
Size: 5815
BlpI GC'TnA_GC
Cuts at: 3232 3232
Size: 5815
Bme1580I G_kGCm'C
Cuts at: 1790 3015 3876 5032 1790
Size: 1225 861 1156 2573
Fragments arranged by size:
2573 1225 1156 861
BmgBI CAC'GTC
Cuts at: 1318 2299 1318
Size: 981 4834
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3546 4504 442
Size: 416 53 83 399 24 2129 958 1753
Fragments arranged by size:
2129 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5815
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2351 2351
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2346 2574 2642 2933 4475 1239
Size: 1107 228 68 291 1542 2579
Fragments arranged by size:
2579 1542 1107 291 228 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2165 2333 2438 3940 4805 5103 2165
Size: 168 105 1502 865 298 2877
Fragments arranged by size:
2877 1502 865 298 168 105
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2804 4478 882
Size: 617 1305 1674 2219
Fragments arranged by size:
2219 1674 1305 617
BsaAI yAC'GTr
Cuts at: 493 1204 3606 493
Size: 711 2402 2702
Fragments arranged by size:
2702 2402 711
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2277 2573 2646 3622
Size: 53 83 186 936 744 296 73 976
Cuts at: 3622 4004 4639 275
Size: 382 635 1451
Fragments arranged by size:
1451 976 936 744 635 382 296 186
83 73 53
BsaWI w'CCGG_w
Cuts at: 4248 4646 4920 5067 5478 4248
Size: 398 274 147 411 4585
Fragments arranged by size:
4585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5590
Fragments arranged by size:
5590 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 2099 2225 2343 2387 2390 3074 3112
Size: 852 61 126 118 44 3 684 38
Cuts at: 3112 3115 1186
Size: 3 3886
Fragments arranged by size:
3886 852 684 126 118 61 44 38
3 3
BseYI C'CCAG_C
Cuts at: 2089 5018 5514 2089
Size: 2929 496 2390
Fragments arranged by size:
2929 2390 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2234 2255 1163
Size: 573 498 21 4723
Fragments arranged by size:
4723 573 498 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4026 5054 1540
Size: 275 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2138 2307 2337 3015 3876 3961 5032 2138
Size: 169 30 678 861 85 1071 2921
Fragments arranged by size:
2921 1071 861 678 169 85 30
BsmI GAATG_Cn'
Cuts at: 3326 3419 3326
Size: 93 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2307 2337 2613 3015 3134 3876
Size: 399 348 169 30 276 402 119 742
Cuts at: 3876 3961 5032 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 402 399 348 276 169
119 85 30
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 3704 5434 3704
Size: 1730 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2233 3170 844
Size: 1389 937 3489
Fragments arranged by size:
3489 1389 937
BsrBI CCG'CTC
Cuts at: 1394 1823 2221 2994 3702 1394
Size: 429 398 773 708 3507
Fragments arranged by size:
3507 773 708 429 398
BsrDI GCAATG_nn'
Cuts at: 65 1248 4310 4484 65
Size: 1183 3062 174 1396
Fragments arranged by size:
3062 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2190 2863 4459 4646 1700
Size: 110 380 673 1596 187 2869
Fragments arranged by size:
2869 1596 673 380 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5815
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 3875 4887 3875
Size: 1012 4803
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2248 794
Size: 1454 4361
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5815
BstYI r'GATC_y
Cuts at: 1972 2400 3145 3512 3897 3914 5366 5452 5464
Size: 428 745 367 385 17 1452 86 12
Cuts at: 5464 5810 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 745 428 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3017 3035 3017
Size: 18 5797
BtgI C'CryG_G
Cuts at: 513 1065 2883 3092 513
Size: 552 1818 209 3236
Fragments arranged by size:
3236 1818 552 209
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3052 487
Size: 683 56 1826 3250
Fragments arranged by size:
3250 1826 683 56
BtsI GCAGTG_nn'
Cuts at: 2733 3331 4125 4145 2733
Size: 598 794 20 4403
Fragments arranged by size:
4403 794 598 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 3119 3119
Size: 5815
DraIII CAC_nnn'GTG
Cuts at: 2369 2369
Size: 5815
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4822 809
Size: 507 3506 1802
Fragments arranged by size:
3506 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2775 3262
Size: 54 1425 50 275 18 268 677 487
Cuts at: 3262 4151 5780 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 677 487 275 268 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2126 2342 3745 1353
Size: 773 216 1403 3423
Fragments arranged by size:
3423 1403 773 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4402 4776 4922 387
Size: 1064 138 2813 374 146 1280
Fragments arranged by size:
2813 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2335 2335
Size: 5815
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2346 2574 2642 2740 2905 2933 2944
Size: 393 714 228 68 98 165 28 11
Cuts at: 2944 3878 4475 5262 5530 1239
Size: 934 597 787 268 1524
Fragments arranged by size:
1524 934 787 714 597 393 268 228
165 98 68 28 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2020 2240 2020
Size: 220 5595
EcoO109I rG'GnC_Cy
Cuts at: 3031 3031
Size: 5815
EcoRI G'AATT_C
Cuts at: 2750 3124 3502 2750
Size: 374 378 5063
Fragments arranged by size:
5063 378 374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2974 2974
Size: 5815
FspI TGC'GCA
Cuts at: 2891 4321 2891
Size: 1430 4385
HaeII r_GCGC'y
Cuts at: 1678 4962 1678
Size: 3284 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2306 2338 4456 4488 4530
Size: 32 135 32 734 32 2118 32 42
Cuts at: 4530 4562 1373
Size: 32 2626
Fragments arranged by size:
2626 2118 734 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 3405 669
Size: 988 281 1221 246 3079
Fragments arranged by size:
3079 1221 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2795 2951 748
Size: 2047 156 3612
Fragments arranged by size:
3612 2047 156
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 3414 3414
Size: 5815
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4929 5113 1305
Size: 3624 184 2007
Fragments arranged by size:
3624 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2100 2777 10
Size: 54 1425 343 268 677 3048
Fragments arranged by size:
3048 1425 677 343 268 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2256 2297 2726 2864 3773 4132 4291
Size: 648 1090 41 429 138 909 359 159
Cuts at: 4291 518
Size: 2042
Fragments arranged by size:
2042 1090 909 648 429 359 159 138
41
MspA1I CmG'CkG
Cuts at: 1606 3231 3908 5056 5301 1606
Size: 1625 677 1148 245 2120
Fragments arranged by size:
2120 1625 1148 677 245
NaeI GCC'GGC
Cuts at: 2192 2192
Size: 5815
NcoI C'CATG_G
Cuts at: 513 1065 2883 513
Size: 552 1818 3445
Fragments arranged by size:
3445 1818 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NgoMIV G'CCGG_C
Cuts at: 2190 2190
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5815
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 2635 3173 4718 1132
Size: 97 1406 538 1545 2229
Fragments arranged by size:
2229 1545 1406 538 97
PasI CC'CwG_GG
Cuts at: 1348 2614 1348
Size: 1266 4549
PciI A'CATG_T
Cuts at: 2631 4714 2631
Size: 2083 3732
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5815
PfoI T'CCnGG_A
Cuts at: 2403 2403
Size: 5815
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3031 3031
Size: 5815
PshAI GACnn'nnGTC
Cuts at: 1613 2520 1613
Size: 907 4908
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PstI C_TGCA'G
Cuts at: 830 3167 830
Size: 2337 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
SacI G_AGCT'C
Cuts at: 2337 2337
Size: 5815
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SapI GCTCTTCn'nnn_
Cuts at: 2126 2342 2126
Size: 216 5599
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SfcI C'TryA_G
Cuts at: 826 1045 3163 4298 4979 5170 826
Size: 219 2118 1135 681 191 1471
Fragments arranged by size:
2118 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 2863 1700
Size: 1163 4652
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 2312 2453 2665 3919 4820
Size: 197 926 201 168 141 212 1254 901
Cuts at: 4820 5082 820
Size: 262 1553
Fragments arranged by size:
1553 1254 926 901 262 212 201 197
168 141
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 2488 2563 3739 5
Size: 47 2436 75 1176 2081
Fragments arranged by size:
2436 2081 1176 75 47
StuI AGG'CCT
Cuts at: 1517 2015 1517
Size: 498 5317
StyI C'CwwG_G
Cuts at: 513 1065 2778 2883 3254 513
Size: 552 1713 105 371 3074
Fragments arranged by size:
3074 1713 552 371 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3105 3842 3859 4012 4197 4642 325
Size: 1255 1525 737 17 153 185 445 1498
Fragments arranged by size:
1525 1498 1255 737 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4061 5773 96
Size: 274 80 33 51 1443 2084 1712 138
Fragments arranged by size:
2084 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5775 5807 5775
Size: 32 5783
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5815
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5815
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 3944 3944
Size: 5815
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2278 3623 276
Size: 53 83 186 936 744 1345 2468
Fragments arranged by size:
2468 1345 936 744 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI