Restriction map for
pFN21AB6548
(Circular) (Six-base) MAPSORT of: pFN21AB6548.seq Check: 5191 from: 1 to: 5881
pFN21AB6548 5881 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3224 1937
Size: 1287 4594
Acc65I G'GTAC_C
Cuts at: 2411 3202 2411
Size: 791 5090
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2027 2582 2813 3084 3944 5328 5596 1632
Size: 395 555 231 271 860 1384 268 1917
Fragments arranged by size:
1917 1384 860 555 395 271 268 231
AflII C'TTAA_G
Cuts at: 820 1017 2987 820
Size: 197 1970 3714
Fragments arranged by size:
3714 1970 197
AflIII A'CryG_T
Cuts at: 1203 2116 2772 4780 5757 1203
Size: 913 656 2008 977 1327
Fragments arranged by size:
2008 1327 977 913 656
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5881
AlwNI CAG_nnn'CTG
Cuts at: 2134 2245 2429 2560 2950 3624 5196 2134
Size: 111 184 131 390 674 1572 2819
Fragments arranged by size:
2819 1572 674 390 184 131 111
ApaI G_GGCC'C
Cuts at: 2880 3059 2880
Size: 179 5702
ApaLI G'TGCA_C
Cuts at: 3938 5094 3938
Size: 1156 4725
ApoI r'AATT_y
Cuts at: 1493 3190 3416 3568 5677 1493
Size: 1697 226 152 2109 1697
Fragments arranged by size:
2109 1697 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 2180 2734 3206 1870
Size: 73 237 554 472 4545
Fragments arranged by size:
4545 554 472 237 73
BamHI G'GATC_C
Cuts at: 3211 3578 3211
Size: 367 5514
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2411 3202 4657 618
Size: 325 217 627 624 791 1455 1842
Fragments arranged by size:
1842 1455 791 627 624 325 217
BanII G_rGCy'C
Cuts at: 1391 2439 2727 2880 2970 3059 3200 1391
Size: 1048 288 153 90 89 141 4072
Fragments arranged by size:
4072 1048 288 153 141 90 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4910
BbvCI CC'TCA_GC
Cuts at: 2577 2891 2577
Size: 314 5567
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4072 4106 1903
Size: 34 50 34 2051 34 3678
Fragments arranged by size:
3678 2051 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3773 4983 3773
Size: 1210 4671
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5881
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2378 2565 4492
Size: 107 122 71 631 605 706 187 1927
Cuts at: 4492 136
Size: 1525
Fragments arranged by size:
1927 1525 706 631 605 187 122 107
71
BglII A'GATC_T
Cuts at: 5876 5876
Size: 5881
BlpI GC'TnA_GC
Cuts at: 2964 3012 3298 2964
Size: 48 286 5547
Fragments arranged by size:
5547 286 48
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2880 3059 3942 5098 1790
Size: 289 801 179 883 1156 2573
Fragments arranged by size:
2573 1156 883 801 289 179
BmgBI CAC'GTC
Cuts at: 1318 2773 1318
Size: 1455 4426
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3612 4570 442
Size: 416 53 83 399 24 2195 958 1753
Fragments arranged by size:
2195 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2017 4541 1239
Size: 778 2524 2579
Fragments arranged by size:
2579 2524 778
Bpu10I CC'TnA_GC
Cuts at: 2577 2891 2577
Size: 314 5567
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4006 4871 5169 4006
Size: 865 298 4718
Fragments arranged by size:
4718 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2209 4544 882
Size: 617 710 2335 2219
Fragments arranged by size:
2335 2219 710 617
BsaAI yAC'GTr
Cuts at: 493 1204 2119 3672 493
Size: 711 915 1553 2702
Fragments arranged by size:
2702 1553 915 711
BsaBI GATnn'nnATC
Cuts at: 3122 5875 3122
Size: 2753 3128
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2904 3688 4070 4705
Size: 53 83 186 936 1371 784 382 635
Cuts at: 4705 275
Size: 1451
Fragments arranged by size:
1451 1371 936 784 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4314 4712 4986 5133 5544 4314
Size: 398 274 147 411 4651
Fragments arranged by size:
4651 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2759 2789 1178
Size: 30 165 30 1356 30 4270
Fragments arranged by size:
4270 1356 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2243 2341 2531 2606 2609 2612 2717 1186
Size: 1057 98 190 75 3 3 105 4350
Fragments arranged by size:
4350 1057 190 105 98 75 3 3
BseYI C'CCAG_C
Cuts at: 2079 2885 2982 3074 3170 5084 5580 2079
Size: 806 97 92 96 1914 496 2380
Fragments arranged by size:
2380 1914 806 496 97 96 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5308
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2859 3331 4092 5120 1540
Size: 275 186 858 472 761 1028 2301
Fragments arranged by size:
2301 1028 858 761 472 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 3942 4027 5098 2025
Size: 1917 85 1071 2808
Fragments arranged by size:
2808 1917 1071 85
BsmI GAATG_Cn'
Cuts at: 2053 3392 3485 2053
Size: 1339 93 4449
Fragments arranged by size:
4449 1339 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2079 2439 2727 2880 2970 3059
Size: 399 235 54 360 288 153 90 89
Cuts at: 3059 3200 3942 4027 5098 1391
Size: 141 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 360 288 235 153
141 90 89 85 54
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 3770 5500 3770
Size: 1730 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3236 844
Size: 2392 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3768 1394
Size: 429 1945 3507
Fragments arranged by size:
3507 1945 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4376 4550 65
Size: 1183 3128 174 1396
Fragments arranged by size:
3128 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2854 3016 4525 4712 1700
Size: 110 1044 162 1509 187 2869
Fragments arranged by size:
2869 1509 1044 187 162 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5881
BssSI C'ACGA_G
Cuts at: 3941 4953 3941
Size: 1012 4869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5881
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 2140 1184
Size: 956 4925
BstXI CCAn_nnnn'nTGG
Cuts at: 2833 2833
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 2130 2346 2417 3099 3211 3578 3963 3980
Size: 158 216 71 682 112 367 385 17
Cuts at: 3980 5432 5518 5530 5876 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 682 385 367 346 216 158
112 86 71 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2781 3108 513
Size: 552 1716 327 3286
Fragments arranged by size:
3286 1716 552 327
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5142
Fragments arranged by size:
5142 683 56
BtsI GCAGTG_nn'
Cuts at: 3397 4191 4211 3397
Size: 794 20 5067
Fragments arranged by size:
5067 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5846
DraI TTT'AAA
Cuts at: 3185 3185
Size: 5881
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2166 2658 2856
Size: 54 1425 50 275 18 336 492 198
Cuts at: 2856 3328 4217 5846 8
Size: 472 889 1629 43
Fragments arranged by size:
1629 1425 889 492 472 336 275 198
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2856 3328 1537
Size: 275 1044 472 4090
Fragments arranged by size:
4090 1044 472 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2490 2532 2925 3182 3811 1353
Size: 1137 42 393 257 629 3423
Fragments arranged by size:
3423 1137 629 393 257 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2535 4468 4842 4988 387
Size: 1064 138 946 1933 374 146 1280
Fragments arranged by size:
1933 1280 1064 946 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2017 2027 2582 2813 3084 3944 4541
Size: 393 385 10 555 231 271 860 597
Cuts at: 4541 5328 5596 1239
Size: 787 268 1524
Fragments arranged by size:
1524 860 787 597 555 393 385 271
268 231 10
EcoO109I rG'GnC_Cy
Cuts at: 2293 2758 2876 2293
Size: 465 118 5298
Fragments arranged by size:
5298 465 118
EcoRI G'AATT_C
Cuts at: 3190 3568 3190
Size: 378 5503
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 2606 5028 1678
Size: 928 2422 2531
Fragments arranged by size:
2531 2422 928
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2759 2762 2791 2794 4522
Size: 32 135 32 1187 3 29 3 1728
Cuts at: 4522 4554 4596 4628 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1728 1187 135 42 32 32 32
32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5881
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 2415 3206 2415
Size: 791 5090
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4995 5179 1305
Size: 3690 184 2007
Fragments arranged by size:
3690 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4059
Fragments arranged by size:
4059 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3839 4198 4357 518
Size: 648 2673 359 159 2042
Fragments arranged by size:
2673 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3038 3297 3974 5122 5367 1606
Size: 1432 259 677 1148 245 2120
Fragments arranged by size:
2120 1432 1148 677 259 245
NaeI GCC'GGC
Cuts at: 2856 2856
Size: 5881
NcoI C'CATG_G
Cuts at: 513 1065 2781 3108 513
Size: 552 1716 327 3286
Fragments arranged by size:
3286 1716 552 327
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5881
NgoMIV G'CCGG_C
Cuts at: 2854 2854
Size: 5881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NspI r_CATG'y
Cuts at: 1132 1229 3239 4784 1132
Size: 97 2010 1545 2229
Fragments arranged by size:
2229 2010 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2423 2833 1348
Size: 1075 410 4396
Fragments arranged by size:
4396 1075 410
PciI A'CATG_T
Cuts at: 4780 4780
Size: 5881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2134 2200 3109 1108
Size: 1026 66 909 3880
Fragments arranged by size:
3880 1026 909 66
PfoI T'CCnGG_A
Cuts at: 2319 2460 3138 2319
Size: 141 678 5062
Fragments arranged by size:
5062 678 141
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PmlI CAC'GTG
Cuts at: 2119 2119
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 5463 5495 3950
Size: 32 1481 32 4336
Fragments arranged by size:
4336 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2758 2293
Size: 465 5416
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5881
PsiI TTA'TAA
Cuts at: 3451 3451
Size: 5881
PspOMI G'GGCC_C
Cuts at: 2876 3055 2876
Size: 179 5702
PstI C_TGCA'G
Cuts at: 830 2434 3233 830
Size: 1604 799 3478
Fragments arranged by size:
3478 1604 799
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
PvuII CAG'CTG
Cuts at: 3038 3038
Size: 5881
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SfcI C'TryA_G
Cuts at: 826 1045 2430 3229 4364 5045 5236 826
Size: 219 1385 799 1135 681 191 1471
Fragments arranged by size:
1471 1385 1135 799 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 2736 3208 1872
Size: 864 472 4545
Fragments arranged by size:
4545 864 472
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2987 3985 4886 5148 820
Size: 197 926 1044 998 901 262 1553
Fragments arranged by size:
1553 1044 998 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 3239 1132
Size: 2107 3774
SrfI GCCC'GGGC
Cuts at: 2736 2736
Size: 5881
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5881
StyI C'CwwG_G
Cuts at: 513 1065 2364 2586 2781 2910 3108 3320 513
Size: 552 1299 222 195 129 198 212 3074
Fragments arranged by size:
3074 1299 552 222 212 198 195 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2825 2899 3908 3925 4078 4263 4708
Size: 1255 1245 74 1009 17 153 185 445
Cuts at: 4708 325
Size: 1498
Fragments arranged by size:
1498 1255 1245 1009 445 185 153 74
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 3132 4127
Size: 274 80 33 51 1443 237 918 995
Cuts at: 4127 5839 96
Size: 1712 138
Fragments arranged by size:
1712 1443 995 918 274 237 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5841 5873 5841
Size: 32 5849
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5881
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3001 3115 3001
Size: 114 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5881
XmaI C'CCGG_G
Cuts at: 1870 2734 3206 1870
Size: 864 472 4545
Fragments arranged by size:
4545 864 472
XmnI GAAnn'nnTTC
Cuts at: 4010 4010
Size: 5881
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SwaI