Restriction map for
pFN21AB6547
(Circular) (Six-base) MAPSORT of: pFN21AB6547.seq Check: 9532 from: 1 to: 5815
pFN21AB6547 5815 bp
With 182 enzymes: SgfI *
October 20, 2009 10:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3625 278
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3158 1937
Size: 1221 4594
Acc65I G'GTAC_C
Cuts at: 3136 3136
Size: 5815
AclI AA'CG_TT
Cuts at: 1563 3942 4315 1563
Size: 2379 373 3063
Fragments arranged by size:
3063 2379 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2876 3878 5262 5530 1632
Size: 1244 1002 1384 268 1917
Fragments arranged by size:
1917 1384 1244 1002 268
AflII C'TTAA_G
Cuts at: 820 1017 2993 820
Size: 197 1976 3642
Fragments arranged by size:
3642 1976 197
AflIII A'CryG_T
Cuts at: 1203 2440 4714 5691 1203
Size: 1237 2274 977 1327
Fragments arranged by size:
2274 1327 1237 977
AgeI A'CCGG_T
Cuts at: 4646 4646
Size: 5815
AhdI GACnn_n'nnGTC
Cuts at: 4544 4544
Size: 5815
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5815
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5815
AlwNI CAG_nnn'CTG
Cuts at: 3558 5130 3558
Size: 1572 4243
ApaLI G'TGCA_C
Cuts at: 2151 3872 5028 2151
Size: 1721 1156 2938
Fragments arranged by size:
2938 1721 1156
ApoI r'AATT_y
Cuts at: 1493 2143 2240 2309 2323 2658 3124 3350 3502
Size: 650 97 69 14 335 466 226 152
Cuts at: 3502 5611 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 650 466 335 226 152 97
69 14
AseI AT'TA_AT
Cuts at: 160 1032 2666 4369 160
Size: 872 1634 1703 1606
Fragments arranged by size:
1703 1634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
AvaI C'yCGr_G
Cuts at: 1870 1943 2052 3140 1870
Size: 73 109 1088 4545
Fragments arranged by size:
4545 1088 109 73
BamHI G'GATC_C
Cuts at: 3145 3512 3145
Size: 367 5448
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3136 4591 618
Size: 325 217 627 1349 1455 1842
Fragments arranged by size:
1842 1455 1349 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2947 3134 1391
Size: 1556 187 4072
Fragments arranged by size:
4072 1556 187
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2050 2682 928
Size: 971 151 632 4061
Fragments arranged by size:
4061 971 632 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4006 4040 1903
Size: 34 2069 34 3678
Fragments arranged by size:
3678 2069 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3707 4917 3707
Size: 1210 4605
BclI T'GATC_A
Cuts at: 1548 2974 1548
Size: 1426 4389
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4426 136
Size: 107 122 71 631 605 2754 1525
Fragments arranged by size:
2754 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5810 5810
Size: 5815
BlpI GC'TnA_GC
Cuts at: 3232 3232
Size: 5815
Bme1580I G_kGCm'C
Cuts at: 1790 2155 3876 5032 1790
Size: 365 1721 1156 2573
Fragments arranged by size:
2573 1721 1156 365
BmgBI CAC'GTC
Cuts at: 1318 2068 1318
Size: 750 5065
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2037 2199 2728
Size: 416 53 83 399 24 620 162 529
Cuts at: 2728 3546 4504 442
Size: 818 958 1753
Fragments arranged by size:
1753 958 818 620 529 416 399 162
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4475 1239
Size: 3236 2579
Bpu10I CC'TnA_GC
Cuts at: 3003 3003
Size: 5815
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2931 3940 4805 5103 2931
Size: 1009 865 298 3643
Fragments arranged by size:
3643 1009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4478 882
Size: 617 2979 2219
Fragments arranged by size:
2979 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2761 3606 493
Size: 711 1557 845 2702
Fragments arranged by size:
2702 1557 845 711
BsaBI GATnn'nnATC
Cuts at: 5809 5809
Size: 5815
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3622 4004 4639 275
Size: 53 83 186 936 2089 382 635 1451
Fragments arranged by size:
2089 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4248 4646 4920 5067 5478 4248
Size: 398 274 147 411 4585
Fragments arranged by size:
4585 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5590
Fragments arranged by size:
5590 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2412 1186
Size: 1226 4589
BseYI C'CCAG_C
Cuts at: 5018 5514 5018
Size: 496 5319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5242
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3265 4026 5054 1540
Size: 275 186 1264 761 1028 2301
Fragments arranged by size:
2301 1264 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2155 2947 3876 3961 5032 2155
Size: 792 929 85 1071 2938
Fragments arranged by size:
2938 1071 929 792 85
BsmI GAATG_Cn'
Cuts at: 3326 3419 3326
Size: 93 5722
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2155 2947 3134 3876 3961 5032 1391
Size: 399 365 792 187 742 85 1071 2174
Fragments arranged by size:
2174 1071 792 742 399 365 187 85
BspEI T'CCGG_A
Cuts at: 5478 5478
Size: 5815
BspHI T'CATG_A
Cuts at: 3704 5434 3704
Size: 1730 4085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2527 3170 844
Size: 1683 643 3489
Fragments arranged by size:
3489 1683 643
BsrBI CCG'CTC
Cuts at: 1394 1823 3702 1394
Size: 429 1879 3507
Fragments arranged by size:
3507 1879 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4310 4484 65
Size: 1183 3062 174 1396
Fragments arranged by size:
3062 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4459 4646 1700
Size: 110 2649 187 2869
Fragments arranged by size:
2869 2649 187 110
BsrGI T'GTAC_A
Cuts at: 96 2159 96
Size: 2063 3752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5815
BssSI C'ACGA_G
Cuts at: 3875 4887 3875
Size: 1012 4803
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3098 794
Size: 2304 3511
BstBI TT'CG_AA
Cuts at: 4619 4619
Size: 5815
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5815
BstYI r'GATC_y
Cuts at: 1972 3145 3512 3897 3914 5366 5452 5464 5810
Size: 1173 367 385 17 1452 86 12 346
Cuts at: 5810 1972
Size: 1977
Fragments arranged by size:
1977 1452 1173 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2622 2622
Size: 5815
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5076
Fragments arranged by size:
5076 683 56
BtsI GCAGTG_nn'
Cuts at: 3331 4125 4145 3331
Size: 794 20 5001
Fragments arranged by size:
5001 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5815
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5780
DraI TTT'AAA
Cuts at: 2643 3119 2643
Size: 476 5339
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4822 809
Size: 507 3506 1802
Fragments arranged by size:
3506 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2452 3262 4151
Size: 54 1425 50 275 18 622 810 889
Cuts at: 4151 5780 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 810 622 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3262 1537
Size: 275 1450 4090
Fragments arranged by size:
4090 1450 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3745 1353
Size: 2392 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4402 4776 4922 387
Size: 1064 138 2813 374 146 1280
Fragments arranged by size:
2813 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2945 2945
Size: 5815
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2876 3878 4475 5262 5530 1239
Size: 393 1244 1002 597 787 268 1524
Fragments arranged by size:
1524 1244 1002 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3124 3502 3124
Size: 378 5437
EcoRV GAT'ATC
Cuts at: 2122 2122
Size: 5815
FspI TGC'GCA
Cuts at: 4321 4321
Size: 5815
HaeII r_GCGC'y
Cuts at: 1678 4962 1678
Size: 3284 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4456 4488 4530 4562 1373
Size: 32 135 32 2884 32 42 32 2626
Fragments arranged by size:
2884 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3159 3405 669
Size: 988 281 1221 246 3079
Fragments arranged by size:
3079 1221 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5815
HpaI GTT'AAC
Cuts at: 3405 3405
Size: 5815
KpnI G_GTAC'C
Cuts at: 3140 3140
Size: 5815
MfeI C'AATT_G
Cuts at: 2880 3414 2880
Size: 534 5281
MluI A'CGCG_T
Cuts at: 5691 5691
Size: 5815
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2061 2441 4929 5113 1305
Size: 756 380 2488 184 2007
Fragments arranged by size:
2488 2007 756 380 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2454 10
Size: 54 1425 343 622 3371
Fragments arranged by size:
3371 1425 622 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2430 3773 4132 4291 518
Size: 648 1264 1343 359 159 2042
Fragments arranged by size:
2042 1343 1264 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3231 3908 5056 5301 1606
Size: 1625 677 1148 245 2120
Fragments arranged by size:
2120 1625 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5263
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5815
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5815
NotI GC'GGCC_GC
Cuts at: 3262 3262
Size: 5815
NspI r_CATG'y
Cuts at: 1132 1229 2444 3173 4718 1132
Size: 97 1215 729 1545 2229
Fragments arranged by size:
2229 1545 1215 729 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5815
PciI A'CATG_T
Cuts at: 2440 4714 2440
Size: 2274 3541
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2591 1108
Size: 1483 4332
PmeI GTTT'AAAC
Cuts at: 3119 3119
Size: 5815
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3884 3916 5397 5429 3884
Size: 32 1481 32 4270
Fragments arranged by size:
4270 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5815
PsiI TTA'TAA
Cuts at: 3385 3385
Size: 5815
PspXI vC'TCGA_Gb
Cuts at: 2052 2052
Size: 5815
PstI C_TGCA'G
Cuts at: 830 3167 830
Size: 2337 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5815
SacI G_AGCT'C
Cuts at: 2947 2947
Size: 5815
SalI G'TCGA_C
Cuts at: 1936 3157 1936
Size: 1221 4594
SbfI CC_TGCA'GG
Cuts at: 3167 3167
Size: 5815
ScaI AGT'ACT
Cuts at: 4063 5775 4063
Size: 1712 4103
SfcI C'TryA_G
Cuts at: 826 1045 2814 3163 4298 4979 5170 826
Size: 219 1769 349 1135 681 191 1471
Fragments arranged by size:
1769 1471 1135 681 349 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5815
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5815
SmaI CCC'GGG
Cuts at: 1872 3142 1872
Size: 1270 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2052 2946 2993 3919 4820 5082
Size: 197 926 109 894 47 926 901 262
Cuts at: 5082 820
Size: 1553
Fragments arranged by size:
1553 926 926 901 894 262 197 109
47
SnaBI TAC'GTA
Cuts at: 493 3606 493
Size: 3113 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5815
SphI G_CATG'C
Cuts at: 1132 3173 1132
Size: 2041 3774
SspI AAT'ATT
Cuts at: 5 52 2580 2839 3739 5
Size: 47 2528 259 900 2081
Fragments arranged by size:
2528 2081 900 259 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5815
StyI C'CwwG_G
Cuts at: 513 1065 3254 513
Size: 552 2189 3074
Fragments arranged by size:
3074 2189 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3842 3859 4012 4197 4642 325
Size: 1255 2262 17 153 185 445 1498
Fragments arranged by size:
2262 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2159 2830 4061
Size: 274 80 33 51 1443 182 671 1231
Cuts at: 4061 5773 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1231 671 274 182 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2398 2430 2460 2492 5775 5807 2398
Size: 32 30 32 3283 32 2406
Fragments arranged by size:
3283 2406 32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5815
XbaI T'CTAG_A
Cuts at: 3151 3151
Size: 5815
XhoI C'TCGA_G
Cuts at: 1943 2052 1943
Size: 109 5706
XmaI C'CCGG_G
Cuts at: 1870 3140 1870
Size: 1270 4545
XmnI GAAnn'nnTTC
Cuts at: 2145 3944 2145
Size: 1799 4016
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3623 276
Size: 53 83 186 936 2089 2468
Fragments arranged by size:
2468 2089 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI