Restriction map for
pFN21AB6546
(Circular) (Six-base) MAPSORT of: pFN21AB6546.seq Check: 5974 from: 1 to: 5881
pFN21AB6546 5881 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2431 2431
Size: 5881
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3691 278
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3224 1937
Size: 1287 4594
Acc65I G'GTAC_C
Cuts at: 3202 3202
Size: 5881
AclI AA'CG_TT
Cuts at: 1563 4008 4381 1563
Size: 2445 373 3063
Fragments arranged by size:
3063 2445 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2525 2636 2702 3944 5328 5596 1632
Size: 893 111 66 1242 1384 268 1917
Fragments arranged by size:
1917 1384 1242 893 268 111 66
AflII C'TTAA_G
Cuts at: 820 1017 2159 820
Size: 197 1142 4542
Fragments arranged by size:
4542 1142 197
AflIII A'CryG_T
Cuts at: 1203 4780 5757 1203
Size: 3577 977 1327
Fragments arranged by size:
3577 1327 977
AgeI A'CCGG_T
Cuts at: 4712 4712
Size: 5881
AhdI GACnn_n'nnGTC
Cuts at: 4610 4610
Size: 5881
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5881
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2837 1184
Size: 1653 4228
AlwNI CAG_nnn'CTG
Cuts at: 3624 5196 3624
Size: 1572 4309
ApaLI G'TGCA_C
Cuts at: 3938 5094 3938
Size: 1156 4725
ApoI r'AATT_y
Cuts at: 1493 2452 2833 3028 3190 3416 3568 5677 1493
Size: 959 381 195 162 226 152 2109 1697
Fragments arranged by size:
2109 1697 959 381 226 195 162 152
AseI AT'TA_AT
Cuts at: 160 1032 4435 160
Size: 872 3403 1606
Fragments arranged by size:
3403 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
AvaI C'yCGr_G
Cuts at: 1870 1943 3206 1870
Size: 73 1263 4545
Fragments arranged by size:
4545 1263 73
BamHI G'GATC_C
Cuts at: 2593 3211 3578 2593
Size: 618 367 4896
Fragments arranged by size:
4896 618 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3202 4657 618
Size: 325 217 627 1415 1455 1842
Fragments arranged by size:
1842 1455 1415 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3200 1391
Size: 1809 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2148 2613 2802 3045 928
Size: 971 249 465 189 243 3764
Fragments arranged by size:
3764 971 465 249 243 189
BbvCI CC'TCA_GC
Cuts at: 2417 2417
Size: 5881
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2283 2317 4072 4106 1903
Size: 34 346 34 1755 34 3678
Fragments arranged by size:
3678 1755 346 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3773 4983 3773
Size: 1210 4671
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5881
BfrBI ATG'CAT
Cuts at: 2288 2669 2288
Size: 381 5500
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4492 136
Size: 107 122 71 631 605 2820 1525
Fragments arranged by size:
2820 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2626 5876 2626
Size: 3250 2631
BlpI GC'TnA_GC
Cuts at: 3298 3298
Size: 5881
Bme1580I G_kGCm'C
Cuts at: 1790 2779 3942 5098 1790
Size: 989 1163 1156 2573
Fragments arranged by size:
2573 1163 1156 989
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2485 2683 3612
Size: 416 53 83 399 24 1068 198 929
Cuts at: 3612 4570 442
Size: 958 1753
Fragments arranged by size:
1753 1068 958 929 416 399 198 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 2396 4541 1239
Size: 822 335 2145 2579
Fragments arranged by size:
2579 2145 822 335
Bpu10I CC'TnA_GC
Cuts at: 2417 2417
Size: 5881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2700 4006 4871 5169 2700
Size: 1306 865 298 3412
Fragments arranged by size:
3412 1306 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4544 882
Size: 617 3045 2219
Fragments arranged by size:
3045 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3672 493
Size: 711 2468 2702
Fragments arranged by size:
2702 2468 711
BsaBI GATnn'nnATC
Cuts at: 2598 5875 2598
Size: 3277 2604
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3688 4070 4705 275
Size: 53 83 186 936 2155 382 635 1451
Fragments arranged by size:
2155 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4314 4712 4986 5133 5544 4314
Size: 398 274 147 411 4651
Fragments arranged by size:
4651 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2059 2089 1178
Size: 30 165 30 656 30 4970
Fragments arranged by size:
4970 656 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 1186
Size: 1213 4668
BseYI C'CCAG_C
Cuts at: 5084 5580 5084
Size: 496 5385
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2807 1163
Size: 573 1071 4237
Fragments arranged by size:
4237 1071 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3331 4092 5120 1540
Size: 275 186 1330 761 1028 2301
Fragments arranged by size:
2301 1330 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3942 4027 5098 3942
Size: 85 1071 4725
Fragments arranged by size:
4725 1071 85
BsmI GAATG_Cn'
Cuts at: 3392 3485 3392
Size: 93 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2779 3200 3942 4027 5098 1391
Size: 399 989 421 742 85 1071 2174
Fragments arranged by size:
2174 1071 989 742 421 399 85
BspEI T'CCGG_A
Cuts at: 5544 5544
Size: 5881
BspHI T'CATG_A
Cuts at: 2044 3770 5500 2044
Size: 1726 1730 2425
Fragments arranged by size:
2425 1730 1726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2431 3236 844
Size: 1587 805 3489
Fragments arranged by size:
3489 1587 805
BsrBI CCG'CTC
Cuts at: 1394 1823 3768 1394
Size: 429 1945 3507
Fragments arranged by size:
3507 1945 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4376 4550 65
Size: 1183 3128 174 1396
Fragments arranged by size:
3128 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4525 4712 1700
Size: 110 2715 187 2869
Fragments arranged by size:
2869 2715 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5881
BssSI C'ACGA_G
Cuts at: 3941 4953 3941
Size: 1012 4869
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5881
BstBI TT'CG_AA
Cuts at: 4685 4685
Size: 5881
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5881
BstYI r'GATC_y
Cuts at: 1972 2508 2593 2626 2975 3211 3578 3963 3980
Size: 536 85 33 349 236 367 385 17
Cuts at: 3980 5432 5518 5530 5876 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 536 385 367 349 346 236
86 85 33 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2253 2512 513
Size: 552 939 249 259 3882
Fragments arranged by size:
3882 939 552 259 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5142
Fragments arranged by size:
5142 683 56
BtsI GCAGTG_nn'
Cuts at: 2130 2797 3134 3397 4191 4211 2130
Size: 667 337 263 794 20 3800
Fragments arranged by size:
3800 794 667 337 263 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5881
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2235 2270 529
Size: 35 1671 35 4140
Fragments arranged by size:
4140 1671 35 35
DraI TTT'AAA
Cuts at: 2457 3185 2457
Size: 728 5153
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4888 809
Size: 507 3572 1802
Fragments arranged by size:
3572 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3328 4217 5846
Size: 54 1425 50 275 18 1498 889 1629
Cuts at: 5846 8
Size: 43
Fragments arranged by size:
1629 1498 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3328 1537
Size: 275 1516 4090
Fragments arranged by size:
4090 1516 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2189 2318 2612 2678 2800 3811 1353
Size: 836 129 294 66 122 1011 3423
Fragments arranged by size:
3423 1011 836 294 129 122 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4468 4842 4988 387
Size: 1064 138 2879 374 146 1280
Fragments arranged by size:
2879 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2061 2396 2525 2636 2702 3944 4541
Size: 393 429 335 129 111 66 1242 597
Cuts at: 4541 5328 5596 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1242 787 597 429 393 335 268
129 111 66
EcoNI CCTnn'n_nnAGG
Cuts at: 2913 2913
Size: 5881
EcoRI G'AATT_C
Cuts at: 3190 3568 3190
Size: 378 5503
EcoRV GAT'ATC
Cuts at: 2029 2029
Size: 5881
FspI TGC'GCA
Cuts at: 4387 4387
Size: 5881
HaeII r_GCGC'y
Cuts at: 1678 5028 1678
Size: 3350 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2611 2643 4522 4554 4596
Size: 32 135 32 1039 32 1879 32 42
Cuts at: 4596 4628 1373
Size: 32 2626
Fragments arranged by size:
2626 1879 1039 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3225 3471 669
Size: 988 281 1287 246 3079
Fragments arranged by size:
3079 1287 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5881
HpaI GTT'AAC
Cuts at: 3471 3471
Size: 5881
KpnI G_GTAC'C
Cuts at: 3206 3206
Size: 5881
MfeI C'AATT_G
Cuts at: 3480 3480
Size: 5881
MluI A'CGCG_T
Cuts at: 5757 5757
Size: 5881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4995 5179 1305
Size: 3690 184 2007
Fragments arranged by size:
3690 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4059
Fragments arranged by size:
4059 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3839 4198 4357 518
Size: 648 2673 359 159 2042
Fragments arranged by size:
2673 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2416 3297 3974 5122 5367 1606
Size: 810 881 677 1148 245 2120
Fragments arranged by size:
2120 1148 881 810 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2512 513
Size: 552 939 508 3882
Fragments arranged by size:
3882 939 552 508
NdeI CA'TA_TG
Cuts at: 387 2137 387
Size: 1750 4131
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5881
NotI GC'GGCC_GC
Cuts at: 3328 3328
Size: 5881
NsiI A_TGCA'T
Cuts at: 2290 2671 2290
Size: 381 5500
NspI r_CATG'y
Cuts at: 1132 1229 2669 3000 3239 4784 1132
Size: 97 1440 331 239 1545 2229
Fragments arranged by size:
2229 1545 1440 331 239 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5881
PciI A'CATG_T
Cuts at: 4780 4780
Size: 5881
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2518 1108
Size: 1410 4471
PmeI GTTT'AAAC
Cuts at: 3185 3185
Size: 5881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3950 3982 5463 5495 3950
Size: 32 1481 32 4336
Fragments arranged by size:
4336 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5881
PsiI TTA'TAA
Cuts at: 3174 3451 3174
Size: 277 5604
PstI C_TGCA'G
Cuts at: 830 3233 830
Size: 2403 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5881
PvuII CAG'CTG
Cuts at: 2416 2416
Size: 5881
SalI G'TCGA_C
Cuts at: 1936 3223 1936
Size: 1287 4594
SapI GCTCTTCn'nnn_
Cuts at: 2318 2612 2318
Size: 294 5587
SbfI CC_TGCA'GG
Cuts at: 3233 3233
Size: 5881
ScaI AGT'ACT
Cuts at: 4129 5841 4129
Size: 1712 4169
SfcI C'TryA_G
Cuts at: 826 1045 2573 2781 3229 4364 5045 5236 826
Size: 219 1528 208 448 1135 681 191 1471
Fragments arranged by size:
1528 1471 1135 681 448 219 208 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5881
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5881
SmaI CCC'GGG
Cuts at: 1872 3208 1872
Size: 1336 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 2715 3985 4886 5148 820
Size: 197 926 216 556 1270 901 262 1553
Fragments arranged by size:
1553 1270 926 901 556 262 216 197
SnaBI TAC'GTA
Cuts at: 493 3672 493
Size: 3179 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5881
SphI G_CATG'C
Cuts at: 1132 2669 3239 1132
Size: 1537 570 3774
Fragments arranged by size:
3774 1537 570
SspI AAT'ATT
Cuts at: 5 52 3805 5
Size: 47 3753 2081
Fragments arranged by size:
3753 2081 47
StuI AGG'CCT
Cuts at: 1517 2279 1517
Size: 762 5119
StyI C'CwwG_G
Cuts at: 513 1065 2004 2512 2800 3320 513
Size: 552 939 508 288 520 3074
Fragments arranged by size:
3074 939 552 520 508 288
SwaI ATTT'AAAT
Cuts at: 2457 2457
Size: 5881
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3908 3925 4078 4263 4708 325
Size: 1255 2328 17 153 185 445 1498
Fragments arranged by size:
2328 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2719 4127 5839
Size: 274 80 33 51 1443 742 1408 1712
Cuts at: 5839 96
Size: 138
Fragments arranged by size:
1712 1443 1408 742 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2842 2874 5841 5873 2842
Size: 32 2967 32 2850
Fragments arranged by size:
2967 2850 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5881
XbaI T'CTAG_A
Cuts at: 3217 3217
Size: 5881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5881
XmaI C'CCGG_G
Cuts at: 1870 3206 1870
Size: 1336 4545
XmnI GAAnn'nnTTC
Cuts at: 4010 4010
Size: 5881
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3689 276
Size: 53 83 186 936 2155 2468
Fragments arranged by size:
2468 2155 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI