Restriction map for
pFN21AB6543
(Circular) (Six-base) MAPSORT of: pFN21AB6543.seq Check: 934 from: 1 to: 5812
pFN21AB6543 5812 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3622 278
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3155 1937
Size: 1218 4594
Acc65I G'GTAC_C
Cuts at: 3133 3133
Size: 5812
AclI AA'CG_TT
Cuts at: 1563 3939 4312 1563
Size: 2376 373 3063
Fragments arranged by size:
3063 2376 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2349 2903 3875 5259 5527 1632
Size: 717 554 972 1384 268 1917
Fragments arranged by size:
1917 1384 972 717 554 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5615
AflIII A'CryG_T
Cuts at: 1203 2661 4711 5688 1203
Size: 1458 2050 977 1327
Fragments arranged by size:
2050 1458 1327 977
AgeI A'CCGG_T
Cuts at: 4643 4643
Size: 5812
AhdI GACnn_n'nnGTC
Cuts at: 4541 4541
Size: 5812
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5812
AlwNI CAG_nnn'CTG
Cuts at: 2455 3555 5127 2455
Size: 1100 1572 3140
Fragments arranged by size:
3140 1572 1100
ApaI G_GGCC'C
Cuts at: 3028 3082 3028
Size: 54 5758
ApaLI G'TGCA_C
Cuts at: 3869 5025 3869
Size: 1156 4656
ApoI r'AATT_y
Cuts at: 1493 2871 3121 3347 3499 5608 1493
Size: 1378 250 226 152 2109 1697
Fragments arranged by size:
2109 1697 1378 250 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4366 160
Size: 872 3334 1606
Fragments arranged by size:
3334 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
AvaI C'yCGr_G
Cuts at: 1870 1943 3137 1870
Size: 73 1194 4545
Fragments arranged by size:
4545 1194 73
BamHI G'GATC_C
Cuts at: 3142 3509 3142
Size: 367 5445
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3133 4588 618
Size: 325 217 627 1346 1455 1842
Fragments arranged by size:
1842 1455 1346 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2244 2473 3028 3082 3131 1391
Size: 853 229 555 54 49 4072
Fragments arranged by size:
4072 853 555 229 54 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2112 928
Size: 971 213 4628
Fragments arranged by size:
4628 971 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4003 4037 1903
Size: 34 2066 34 3678
Fragments arranged by size:
3678 2066 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3704 4914 3704
Size: 1210 4602
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5812
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4423 136
Size: 107 122 71 631 605 2751 1525
Fragments arranged by size:
2751 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5807 5807
Size: 5812
BlpI GC'TnA_GC
Cuts at: 2706 3229 2706
Size: 523 5289
Bme1580I G_kGCm'C
Cuts at: 1790 3028 3082 3873 5029 1790
Size: 1238 54 791 1156 2573
Fragments arranged by size:
2573 1238 1156 791 54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5812
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2590 2749 3085
Size: 416 53 83 399 24 1173 159 336
Cuts at: 3085 3543 4501 442
Size: 458 958 1753
Fragments arranged by size:
1753 1173 958 458 416 399 336 159
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2384 2457 2953 4472 1239
Size: 1145 73 496 1519 2579
Fragments arranged by size:
2579 1519 1145 496 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3937 4802 5100 3937
Size: 865 298 4649
Fragments arranged by size:
4649 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4475 882
Size: 617 2976 2219
Fragments arranged by size:
2976 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3603 493
Size: 711 2399 2702
Fragments arranged by size:
2702 2399 711
BsaBI GATnn'nnATC
Cuts at: 5806 5806
Size: 5812
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2090 3619 4001 4636
Size: 53 83 186 936 557 1529 382 635
Cuts at: 4636 275
Size: 1451
Fragments arranged by size:
1529 1451 936 635 557 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2571 4245 4643 4917 5064 5475 2571
Size: 1674 398 274 147 411 2908
Fragments arranged by size:
2908 1674 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5587
Fragments arranged by size:
5587 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5812
BseYI C'CCAG_C
Cuts at: 2059 2244 2508 5015 5511 2059
Size: 185 264 2507 496 2360
Fragments arranged by size:
2507 2360 496 264 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2329 2585 1163
Size: 573 593 256 4390
Fragments arranged by size:
4390 593 573 256
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3262 4023 5051 1540
Size: 275 186 1261 761 1028 2301
Fragments arranged by size:
2301 1261 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2559 2707 3873 3958 5029 2559
Size: 148 1166 85 1071 3342
Fragments arranged by size:
3342 1166 1071 148 85
BsmI GAATG_Cn'
Cuts at: 3323 3416 3323
Size: 93 5719
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2473 2559 2707 3028 3082 3131
Size: 399 454 229 86 148 321 54 49
Cuts at: 3131 3873 3958 5029 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 321 229 148
86 85 54 49
BspEI T'CCGG_A
Cuts at: 5475 5475
Size: 5812
BspHI T'CATG_A
Cuts at: 3701 5431 3701
Size: 1730 4082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3167 844
Size: 2323 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3699 1394
Size: 429 1876 3507
Fragments arranged by size:
3507 1876 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4307 4481 65
Size: 1183 3059 174 1396
Fragments arranged by size:
3059 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4456 4643 1700
Size: 110 2646 187 2869
Fragments arranged by size:
2869 2646 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5812
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5812
BssSI C'ACGA_G
Cuts at: 3872 4884 3872
Size: 1012 4800
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2563 794
Size: 1769 4043
BstBI TT'CG_AA
Cuts at: 4616 4616
Size: 5812
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5812
BstXI CCAn_nnnn'nTGG
Cuts at: 2739 2739
Size: 5812
BstYI r'GATC_y
Cuts at: 1972 2367 2896 3142 3509 3894 3911 5363 5449
Size: 395 529 246 367 385 17 1452 86
Cuts at: 5449 5461 5807 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 529 395 385 367 346 246
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2408 2790 2811 2914 513
Size: 552 1343 382 21 103 3411
Fragments arranged by size:
3411 1343 552 382 103 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5073
Fragments arranged by size:
5073 683 56
BtsI GCAGTG_nn'
Cuts at: 3328 4122 4142 3328
Size: 794 20 4998
Fragments arranged by size:
4998 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5812
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5777
DraI TTT'AAA
Cuts at: 3116 3116
Size: 5812
DraIII CAC_nnn'GTG
Cuts at: 2563 2696 2563
Size: 133 5679
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4819 809
Size: 507 3503 1802
Fragments arranged by size:
3503 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3259 4148 5777
Size: 54 1425 50 275 18 1429 889 1629
Cuts at: 5777 8
Size: 43
Fragments arranged by size:
1629 1429 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3259 1537
Size: 275 1447 4090
Fragments arranged by size:
4090 1447 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3742 1353
Size: 2389 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4399 4773 4919 387
Size: 1064 138 2810 374 146 1280
Fragments arranged by size:
2810 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2349 2384 2457 2903 2953 3875 4472
Size: 393 717 35 73 446 50 922 597
Cuts at: 4472 5259 5527 1239
Size: 787 268 1524
Fragments arranged by size:
1524 922 787 717 597 446 393 268
73 50 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2177 3032 2177
Size: 855 4957
EcoO109I rG'GnC_Cy
Cuts at: 2404 3024 3025 3038 3045 3079 2404
Size: 620 1 13 7 34 5137
Fragments arranged by size:
5137 620 34 13 7 1
EcoRI G'AATT_C
Cuts at: 2871 3121 3499 2871
Size: 250 378 5184
Fragments arranged by size:
5184 378 250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 5812
FspI TGC'GCA
Cuts at: 4318 4318
Size: 5812
HaeII r_GCGC'y
Cuts at: 1678 4959 1678
Size: 3281 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2808 2840 2979 3011 4453
Size: 32 135 32 1236 32 139 32 1442
Cuts at: 4453 4485 4527 4559 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1442 1236 139 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3156 3402 669
Size: 988 281 1218 246 3079
Fragments arranged by size:
3079 1218 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5812
HpaI GTT'AAC
Cuts at: 3402 3402
Size: 5812
KpnI G_GTAC'C
Cuts at: 3137 3137
Size: 5812
MfeI C'AATT_G
Cuts at: 3411 3411
Size: 5812
MluI A'CGCG_T
Cuts at: 5688 5688
Size: 5812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4926 5110 1305
Size: 3621 184 2007
Fragments arranged by size:
3621 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3990
Fragments arranged by size:
3990 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 2715 3770 4129 4288 518
Size: 648 1269 280 1055 359 159 2042
Fragments arranged by size:
2042 1269 1055 648 359 280 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2117 2592 3228 3905 5053 5298 1606
Size: 444 67 475 636 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 636 475 444 245 67
NcoI C'CATG_G
Cuts at: 513 1065 2811 513
Size: 552 1746 3514
Fragments arranged by size:
3514 1746 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5812
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5812
NotI GC'GGCC_GC
Cuts at: 3259 3259
Size: 5812
NspI r_CATG'y
Cuts at: 1132 1229 3170 4715 1132
Size: 97 1941 1545 2229
Fragments arranged by size:
2229 1941 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2466 1348
Size: 1118 4694
PciI A'CATG_T
Cuts at: 4711 4711
Size: 5812
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2551 2696 1108
Size: 1443 145 4224
Fragments arranged by size:
4224 1443 145
PfoI T'CCnGG_A
Cuts at: 2400 2400
Size: 5812
PmeI GTTT'AAAC
Cuts at: 3116 3116
Size: 5812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2219 2251 3881 3913 5394 5426 2219
Size: 32 1630 32 1481 32 2605
Fragments arranged by size:
2605 1630 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2404 2404
Size: 5812
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5812
PsiI TTA'TAA
Cuts at: 3382 3382
Size: 5812
PspOMI G'GGCC_C
Cuts at: 3024 3078 3024
Size: 54 5758
PstI C_TGCA'G
Cuts at: 830 2049 2451 2970 3164 830
Size: 1219 402 519 194 3478
Fragments arranged by size:
3478 1219 519 402 194
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5812
PvuII CAG'CTG
Cuts at: 2050 2117 2592 2050
Size: 67 475 5270
Fragments arranged by size:
5270 475 67
SalI G'TCGA_C
Cuts at: 1936 3154 1936
Size: 1218 4594
SbfI CC_TGCA'GG
Cuts at: 3164 3164
Size: 5812
ScaI AGT'ACT
Cuts at: 4060 5772 4060
Size: 1712 4100
SfcI C'TryA_G
Cuts at: 826 1045 2045 2447 2966 2977 3160 4295 4976
Size: 219 1000 402 519 11 183 1135 681
Cuts at: 4976 5167 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1000 681 519 402 219 191
183 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5812
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5812
SmaI CCC'GGG
Cuts at: 1872 3139 1872
Size: 1267 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3916 4817 5079 820
Size: 197 926 1973 901 262 1553
Fragments arranged by size:
1973 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3603 493
Size: 3110 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5812
SphI G_CATG'C
Cuts at: 1132 3170 1132
Size: 2038 3774
SspI AAT'ATT
Cuts at: 5 52 3736 5
Size: 47 3684 2081
Fragments arranged by size:
3684 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5812
StyI C'CwwG_G
Cuts at: 513 1065 2811 3028 3251 513
Size: 552 1746 217 223 3074
Fragments arranged by size:
3074 1746 552 223 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2745 3839 3856 4009 4194 4639 325
Size: 1255 1165 1094 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1165 1094 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 4058 5770
Size: 274 80 33 51 1443 231 1850 1712
Cuts at: 5770 96
Size: 138
Fragments arranged by size:
1850 1712 1443 274 231 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5772 5804 5772
Size: 32 5780
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5812
XbaI T'CTAG_A
Cuts at: 3148 3148
Size: 5812
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5812
XmaI C'CCGG_G
Cuts at: 1870 3137 1870
Size: 1267 4545
XmnI GAAnn'nnTTC
Cuts at: 2768 3941 2768
Size: 1173 4639
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3620 276
Size: 53 83 186 936 2086 2468
Fragments arranged by size:
2468 2086 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI