Restriction map for
pFN21AB6539
(Circular) (Six-base) MAPSORT of: pFN21AB6539.seq Check: 3626 from: 1 to: 5809
pFN21AB6539 5809 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3619 278
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3152 1937
Size: 1215 4594
Acc65I G'GTAC_C
Cuts at: 3130 3130
Size: 5809
AclI AA'CG_TT
Cuts at: 1563 3936 4309 1563
Size: 2373 373 3063
Fragments arranged by size:
3063 2373 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3872 5256 5524 1632
Size: 2240 1384 268 1917
Fragments arranged by size:
2240 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5612
AflIII A'CryG_T
Cuts at: 1203 4708 5685 1203
Size: 3505 977 1327
Fragments arranged by size:
3505 1327 977
AgeI A'CCGG_T
Cuts at: 4640 4640
Size: 5809
AhdI GACnn_n'nnGTC
Cuts at: 4538 4538
Size: 5809
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5809
AlwNI CAG_nnn'CTG
Cuts at: 2781 3552 5124 2781
Size: 771 1572 3466
Fragments arranged by size:
3466 1572 771
ApaI G_GGCC'C
Cuts at: 2479 2620 2806 2479
Size: 141 186 5482
Fragments arranged by size:
5482 186 141
ApaLI G'TGCA_C
Cuts at: 3866 5022 3866
Size: 1156 4653
ApoI r'AATT_y
Cuts at: 1493 3118 3344 3496 5605 1493
Size: 1625 226 152 2109 1697
Fragments arranged by size:
2109 1697 1625 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4363 160
Size: 872 3331 1606
Fragments arranged by size:
3331 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
AvaI C'yCGr_G
Cuts at: 1870 1943 3134 1870
Size: 73 1191 4545
Fragments arranged by size:
4545 1191 73
BamHI G'GATC_C
Cuts at: 3139 3506 3139
Size: 367 5442
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2216 2414 3130 4585 618
Size: 325 217 627 429 198 716 1455 1842
Fragments arranged by size:
1842 1455 716 627 429 325 217 198
BanII G_rGCy'C
Cuts at: 1391 2341 2479 2516 2620 2806 3068 3128 1391
Size: 950 138 37 104 186 262 60 4072
Fragments arranged by size:
4072 950 262 186 138 104 60 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4000 4034 1903
Size: 34 2063 34 3678
Fragments arranged by size:
3678 2063 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2161 3701 4911 2161
Size: 1540 1210 3059
Fragments arranged by size:
3059 1540 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5809
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4420 136
Size: 107 122 71 631 605 2748 1525
Fragments arranged by size:
2748 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5804 5804
Size: 5809
BlpI GC'TnA_GC
Cuts at: 3226 3226
Size: 5809
Bme1580I G_kGCm'C
Cuts at: 1790 2479 2620 2806 3870 5026 1790
Size: 689 141 186 1064 1156 2573
Fragments arranged by size:
2573 1156 1064 689 186 141
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5809
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2791 3540 4498
Size: 416 53 83 399 24 1374 749 958
Cuts at: 4498 442
Size: 1753
Fragments arranged by size:
1753 1374 958 749 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2419 4469 1239
Size: 1180 2050 2579
Fragments arranged by size:
2579 2050 1180
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2872 3934 4799 5097 2872
Size: 1062 865 298 3584
Fragments arranged by size:
3584 1062 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4472 882
Size: 617 2973 2219
Fragments arranged by size:
2973 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2898 3600 493
Size: 711 1694 702 2702
Fragments arranged by size:
2702 1694 711 702
BsaBI GATnn'nnATC
Cuts at: 2288 5803 2288
Size: 3515 2294
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2196 3616 3998 4633
Size: 53 83 186 936 663 1420 382 635
Cuts at: 4633 275
Size: 1451
Fragments arranged by size:
1451 1420 936 663 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2382 4242 4640 4914 5061 5472 2382
Size: 1860 398 274 147 411 2719
Fragments arranged by size:
2719 1860 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5584
Fragments arranged by size:
5584 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2443 2744 2747 2768 1186
Size: 1257 301 3 21 4227
Fragments arranged by size:
4227 1257 301 21 3
BseYI C'CCAG_C
Cuts at: 2472 2613 5012 5508 2472
Size: 141 2399 496 2773
Fragments arranged by size:
2773 2399 496 141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5236
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3259 4020 5048 1540
Size: 275 186 1258 761 1028 2301
Fragments arranged by size:
2301 1258 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2375 2516 3870 3955 5026 2375
Size: 141 1354 85 1071 3158
Fragments arranged by size:
3158 1354 1071 141 85
BsmI GAATG_Cn'
Cuts at: 3320 3413 3320
Size: 93 5716
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2341 2375 2479 2516 2620 2806 3068
Size: 399 551 34 104 37 104 186 262
Cuts at: 3068 3128 3870 3955 5026 1391
Size: 60 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 551 399 262 186 104
104 85 60 37 34
BspEI T'CCGG_A
Cuts at: 5472 5472
Size: 5809
BspHI T'CATG_A
Cuts at: 2308 3698 5428 2308
Size: 1390 1730 2689
Fragments arranged by size:
2689 1730 1390
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2240 3097 3164 844
Size: 1396 857 67 3489
Fragments arranged by size:
3489 1396 857 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3696 1394
Size: 429 1873 3507
Fragments arranged by size:
3507 1873 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2278 2608 4304 4478 65
Size: 1183 1030 330 1696 174 1396
Fragments arranged by size:
1696 1396 1183 1030 330 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4453 4640 1700
Size: 110 2643 187 2869
Fragments arranged by size:
2869 2643 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5809
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5809
BssSI C'ACGA_G
Cuts at: 2432 3869 4881 2432
Size: 1437 1012 3360
Fragments arranged by size:
3360 1437 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5809
BstBI TT'CG_AA
Cuts at: 4613 4613
Size: 5809
BstEII G'GTnAC_C
Cuts at: 1184 2998 1184
Size: 1814 3995
BstXI CCAn_nnnn'nTGG
Cuts at: 2971 2971
Size: 5809
BstYI r'GATC_y
Cuts at: 1972 2283 3139 3506 3891 3908 5360 5446 5458
Size: 311 856 367 385 17 1452 86 12
Cuts at: 5458 5804 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 856 385 367 346 311 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2300 2300
Size: 5809
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5257
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2818 487
Size: 683 56 1592 3478
Fragments arranged by size:
3478 1592 683 56
BtsI GCAGTG_nn'
Cuts at: 3035 3325 4119 4139 3035
Size: 290 794 20 4705
Fragments arranged by size:
4705 794 290 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5809
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5774
DraI TTT'AAA
Cuts at: 3113 3113
Size: 5809
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2249 2387 4816 809
Size: 507 933 138 2429 1802
Fragments arranged by size:
2429 1802 933 507 138
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2740 3256 4145
Size: 54 1425 50 275 18 910 516 889
Cuts at: 4145 5774 8
Size: 1629 43
Fragments arranged by size:
1629 1425 910 889 516 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3256 1537
Size: 275 1444 4090
Fragments arranged by size:
4090 1444 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 2420 3739 1353
Size: 714 353 1319 3423
Fragments arranged by size:
3423 1319 714 353
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4396 4770 4916 387
Size: 1064 138 2807 374 146 1280
Fragments arranged by size:
2807 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2514 2514
Size: 5809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2419 3872 4469 5256 5524 1239
Size: 393 787 1453 597 787 268 1524
Fragments arranged by size:
1524 1453 787 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3008 3008
Size: 5809
EcoO109I rG'GnC_Cy
Cuts at: 2315 2476 2617 2803 2315
Size: 161 141 186 5321
Fragments arranged by size:
5321 186 161 141
EcoRI G'AATT_C
Cuts at: 3118 3496 3118
Size: 378 5431
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2114 2114
Size: 5809
FspI TGC'GCA
Cuts at: 2270 4315 2270
Size: 2045 3764
HaeII r_GCGC'y
Cuts at: 1678 4956 1678
Size: 3278 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2268 2300 2639 2671 2870
Size: 32 135 32 696 32 339 32 199
Cuts at: 2870 2902 4450 4482 4524 4556 1373
Size: 32 1548 32 42 32 2626
Fragments arranged by size:
2626 1548 696 339 199 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3052 3153 3399 669
Size: 988 281 1114 101 246 3079
Fragments arranged by size:
3079 1114 988 281 246 101
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5809
HpaI GTT'AAC
Cuts at: 3399 3399
Size: 5809
KpnI G_GTAC'C
Cuts at: 3134 3134
Size: 5809
MfeI C'AATT_G
Cuts at: 3408 3408
Size: 5809
MluI A'CGCG_T
Cuts at: 5685 5685
Size: 5809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4923 5107 1305
Size: 3618 184 2007
Fragments arranged by size:
3618 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3987
Fragments arranged by size:
3987 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2436 3767 4126 4285 518
Size: 648 1270 1331 359 159 2042
Fragments arranged by size:
2042 1331 1270 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2613 2841 3225 3902 5050 5295 1606
Size: 1007 228 384 677 1148 245 2120
Fragments arranged by size:
2120 1148 1007 677 384 245 228
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5257
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5809
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5809
NotI GC'GGCC_GC
Cuts at: 3256 3256
Size: 5809
NspI r_CATG'y
Cuts at: 1132 1229 2441 2945 3167 4712 1132
Size: 97 1212 504 222 1545 2229
Fragments arranged by size:
2229 1545 1212 504 222 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5809
PciI A'CATG_T
Cuts at: 4708 4708
Size: 5809
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5809
PmeI GTTT'AAAC
Cuts at: 3113 3113
Size: 5809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3878 3910 5391 5423 3878
Size: 32 1481 32 4264
Fragments arranged by size:
4264 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2315 2315
Size: 5809
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5809
PsiI TTA'TAA
Cuts at: 3379 3379
Size: 5809
PspOMI G'GGCC_C
Cuts at: 2475 2616 2802 2475
Size: 141 186 5482
Fragments arranged by size:
5482 186 141
PstI C_TGCA'G
Cuts at: 830 3161 830
Size: 2331 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5809
PvuII CAG'CTG
Cuts at: 2613 2841 2613
Size: 228 5581
SacI G_AGCT'C
Cuts at: 2516 2516
Size: 5809
SalI G'TCGA_C
Cuts at: 1936 3151 1936
Size: 1215 4594
SapI GCTCTTCn'nnn_
Cuts at: 2420 2420
Size: 5809
SbfI CC_TGCA'GG
Cuts at: 3161 3161
Size: 5809
ScaI AGT'ACT
Cuts at: 4057 5769 4057
Size: 1712 4097
SfcI C'TryA_G
Cuts at: 826 1045 2107 3157 4292 4973 5164 826
Size: 219 1062 1050 1135 681 191 1471
Fragments arranged by size:
1471 1135 1062 1050 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5809
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5809
SmaI CCC'GGG
Cuts at: 1872 3136 1872
Size: 1264 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2887 3913 4814 5076 820
Size: 197 926 944 1026 901 262 1553
Fragments arranged by size:
1553 1026 944 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3600 493
Size: 3107 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5809
SphI G_CATG'C
Cuts at: 1132 2441 3167 1132
Size: 1309 726 3774
Fragments arranged by size:
3774 1309 726
SspI AAT'ATT
Cuts at: 5 52 3733 5
Size: 47 3681 2081
Fragments arranged by size:
3681 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5809
StyI C'CwwG_G
Cuts at: 513 1065 3248 513
Size: 552 2183 3074
Fragments arranged by size:
3074 2183 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2667 3022 3836 3853 4006 4191 4636
Size: 1255 1087 355 814 17 153 185 445
Cuts at: 4636 325
Size: 1498
Fragments arranged by size:
1498 1255 1087 814 445 355 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4055 5767 96
Size: 274 80 33 51 1443 2078 1712 138
Fragments arranged by size:
2078 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2729 2761 5769 5801 2729
Size: 32 3008 32 2737
Fragments arranged by size:
3008 2737 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5809
XbaI T'CTAG_A
Cuts at: 3145 3145
Size: 5809
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5809
XmaI C'CCGG_G
Cuts at: 1870 3134 1870
Size: 1264 4545
XmnI GAAnn'nnTTC
Cuts at: 3938 3938
Size: 5809
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3617 276
Size: 53 83 186 936 2083 2468
Fragments arranged by size:
2468 2083 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI