Restriction map for
pFN21AB6538
(Circular) (Six-base) MAPSORT of: pFN21AB6538.seq Check: 989 from: 1 to: 5878
pFN21AB6538 5878 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3221 1937
Size: 1284 4594
Acc65I G'GTAC_C
Cuts at: 3199 3199
Size: 5878
AclI AA'CG_TT
Cuts at: 1563 4005 4378 1563
Size: 2442 373 3063
Fragments arranged by size:
3063 2442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2517 3941 5325 5593 1632
Size: 885 1424 1384 268 1917
Fragments arranged by size:
1917 1424 1384 885 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 4777 5754 1203
Size: 3574 977 1327
Fragments arranged by size:
3574 1327 977
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 2392 4607 2392
Size: 2215 3663
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5878
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5878
AlwNI CAG_nnn'CTG
Cuts at: 2212 2623 2827 3062 3621 5193 2212
Size: 411 204 235 559 1572 2897
Fragments arranged by size:
2897 1572 559 411 235 204
ApaI G_GGCC'C
Cuts at: 3140 3140
Size: 5878
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 2666 3187 3413 3565 5674 1493
Size: 1173 521 226 152 2109 1697
Fragments arranged by size:
2109 1697 1173 521 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4432 160
Size: 872 3400 1606
Fragments arranged by size:
3400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 3203 1870
Size: 73 1260 4545
Fragments arranged by size:
4545 1260 73
BamHI G'GATC_C
Cuts at: 3208 3575 3208
Size: 367 5511
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3199 4654 618
Size: 325 217 627 1412 1455 1842
Fragments arranged by size:
1842 1455 1412 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2085 2112 2679 2834 2960 3014 3051 3140
Size: 694 27 567 155 126 54 37 89
Cuts at: 3140 3197 1391
Size: 57 4072
Fragments arranged by size:
4072 694 567 155 126 89 57 54
37 27
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2245 2285 2369 2401 3165 928
Size: 971 346 40 84 32 764 3641
Fragments arranged by size:
3641 971 764 346 84 40 32
BbvCI CC'TCA_GC
Cuts at: 2859 2859
Size: 5878
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4069 4103 1903
Size: 34 2132 34 3678
Fragments arranged by size:
3678 2132 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3770 4980 3770
Size: 1210 4668
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4489 136
Size: 107 122 71 631 605 2817 1525
Fragments arranged by size:
2817 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5873 5873
Size: 5878
BlpI GC'TnA_GC
Cuts at: 3045 3295 3045
Size: 250 5628
Bme1580I G_kGCm'C
Cuts at: 1790 2429 3140 3939 5095 1790
Size: 639 711 799 1156 2573
Fragments arranged by size:
2573 1156 799 711 639
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5878
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3045 3609 4567
Size: 416 53 83 399 24 1628 564 958
Cuts at: 4567 442
Size: 1753
Fragments arranged by size:
1753 1628 958 564 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2294 2715 2849 4538 1239
Size: 1055 421 134 1689 2579
Fragments arranged by size:
2579 1689 1055 421 134
Bpu10I CC'TnA_GC
Cuts at: 2681 2859 2681
Size: 178 5700
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2645 4003 4868 5166 2645
Size: 1358 865 298 3357
Fragments arranged by size:
3357 1358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4541 882
Size: 617 3042 2219
Fragments arranged by size:
3042 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3669 493
Size: 711 2465 2702
Fragments arranged by size:
2702 2465 711
BsaBI GATnn'nnATC
Cuts at: 5872 5872
Size: 5878
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3685 4067 4702 275
Size: 53 83 186 936 2152 382 635 1451
Fragments arranged by size:
2152 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2118 4311 4709 4983 5130 5541 2118
Size: 2193 398 274 147 411 2455
Fragments arranged by size:
2455 2193 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 2801 2816 2831
Size: 30 165 30 693 30 675 15 15
Cuts at: 2831 2846 1178
Size: 15 4210
Fragments arranged by size:
4210 693 675 165 30 30 30 15
15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2122 2567 2570 2621 3044 1186
Size: 936 445 3 51 423 4020
Fragments arranged by size:
4020 936 445 423 51 3
BseYI C'CCAG_C
Cuts at: 2416 2446 2506 2997 3140 5081 5577 2416
Size: 30 60 491 143 1941 496 2717
Fragments arranged by size:
2717 1941 496 491 143 60 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2472 1163
Size: 573 736 4569
Fragments arranged by size:
4569 736 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3328 4089 5117 1540
Size: 275 186 1327 761 1028 2301
Fragments arranged by size:
2301 1327 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 2085 3939 4024 5095 2043
Size: 42 1854 85 1071 2826
Fragments arranged by size:
2826 1854 1071 85 42
BsmI GAATG_Cn'
Cuts at: 2715 3389 3482 2715
Size: 674 93 5111
Fragments arranged by size:
5111 674 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2085 2112 2429 2679 2834 2960
Size: 399 253 42 27 317 250 155 126
Cuts at: 2960 3014 3051 3140 3197 3939 4024 5095 1391
Size: 54 37 89 57 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 317 253 250 155
126 89 85 57 54 42 37 27
BspEI T'CCGG_A
Cuts at: 2118 5541 2118
Size: 3423 2455
BspHI T'CATG_A
Cuts at: 3767 5497 3767
Size: 1730 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 3233 844
Size: 1376 1013 3489
Fragments arranged by size:
3489 1376 1013
BsrBI CCG'CTC
Cuts at: 1394 1823 2139 2170 2259 3090 3765 1394
Size: 429 316 31 89 831 675 3507
Fragments arranged by size:
3507 831 675 429 316 89 31
BsrDI GCAATG_nn'
Cuts at: 65 1248 2341 2956 4373 4547 65
Size: 1183 1093 615 1417 174 1396
Fragments arranged by size:
1417 1396 1183 1093 615 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4522 4709 1700
Size: 110 2712 187 2869
Fragments arranged by size:
2869 2712 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5878
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 2422 2549 3938 4950 2422
Size: 127 1389 1012 3350
Fragments arranged by size:
3350 1389 1012 127
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5878
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstXI CCAn_nnnn'nTGG
Cuts at: 2581 2581
Size: 5878
BstYI r'GATC_y
Cuts at: 1972 2226 3208 3575 3960 3977 5429 5515 5527
Size: 254 982 367 385 17 1452 86 12
Cuts at: 5527 5873 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 982 385 367 346 254 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2518 2580 2931 513
Size: 552 1453 62 351 3460
Fragments arranged by size:
3460 1453 552 351 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2282 487
Size: 683 56 1056 4083
Fragments arranged by size:
4083 1056 683 56
BtsI GCAGTG_nn'
Cuts at: 2568 3394 4188 4208 2568
Size: 826 794 20 4238
Fragments arranged by size:
4238 826 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5843
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4885 809
Size: 507 3569 1802
Fragments arranged by size:
3569 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2311 2515 2521
Size: 54 1425 50 275 18 481 204 6
Cuts at: 2521 2643 2776 2790 3325 4214 5843 8
Size: 122 133 14 535 889 1629 43
Fragments arranged by size:
1629 1425 889 535 481 275 204 133
122 54 50 43 18 14 6
EagI C'GGCC_G
Cuts at: 1537 1812 3325 1537
Size: 275 1513 4090
Fragments arranged by size:
4090 1513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2177 3808 1353
Size: 824 1631 3423
Fragments arranged by size:
3423 1631 824
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3170 4465 4839 4985 387
Size: 1064 138 1581 1295 374 146 1280
Fragments arranged by size:
1581 1295 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2083 2083
Size: 5878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2517 2715 2849 3941 4538 5325
Size: 393 662 223 198 134 1092 597 787
Cuts at: 5325 5593 1239
Size: 268 1524
Fragments arranged by size:
1524 1092 787 662 597 393 268 223
198 134
EcoNI CCTnn'n_nnAGG
Cuts at: 2603 2603
Size: 5878
EcoO109I rG'GnC_Cy
Cuts at: 2209 2824 3136 3137 2209
Size: 615 312 1 4950
Fragments arranged by size:
4950 615 312 1
EcoRI G'AATT_C
Cuts at: 2666 3187 3565 2666
Size: 521 378 4979
Fragments arranged by size:
4979 521 378
FspI TGC'GCA
Cuts at: 2540 4384 2540
Size: 1844 4034
HaeII r_GCGC'y
Cuts at: 1678 5025 1678
Size: 3347 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 2378 2405 2410
Size: 32 135 32 524 32 250 27 5
Cuts at: 2410 2437 2795 2801 2816 2827 2833 2848 2900
Size: 27 358 6 15 11 6 15 52
Cuts at: 2900 2932 3001 3033 4519 4551 4593 4625 1373
Size: 32 69 32 1486 32 42 32 2626
Fragments arranged by size:
2626 1486 524 358 250 135 69 52
42 32 32 32 32 32 32 32
27 27 15 15 11 6 6 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3222 3468 669
Size: 988 281 1284 246 3079
Fragments arranged by size:
3079 1284 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5878
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KpnI G_GTAC'C
Cuts at: 3203 3203
Size: 5878
MfeI C'AATT_G
Cuts at: 3477 3477
Size: 5878
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4992 5176 1305
Size: 3687 184 2007
Fragments arranged by size:
3687 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2645 2792 10
Size: 54 1425 343 813 147 3096
Fragments arranged by size:
3096 1425 813 343 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2724 3836 4195 4354 518
Size: 648 1558 1112 359 159 2042
Fragments arranged by size:
2042 1558 1112 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2338 2623 3294 3971 5119 5364 1606
Size: 732 285 671 677 1148 245 2120
Fragments arranged by size:
2120 1148 732 677 671 285 245
NcoI C'CATG_G
Cuts at: 513 1065 2580 2931 513
Size: 552 1515 351 3460
Fragments arranged by size:
3460 1515 552 351
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5878
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 2437 3236 4781 1132
Size: 97 1208 799 1545 2229
Fragments arranged by size:
2229 1545 1208 799 97
PasI CC'CwG_GG
Cuts at: 1348 3133 1348
Size: 1785 4093
PciI A'CATG_T
Cuts at: 4777 4777
Size: 5878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2827 1108
Size: 1719 4159
PfoI T'CCnGG_A
Cuts at: 2382 2382
Size: 5878
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 5460 5492 3947
Size: 32 1481 32 4333
Fragments arranged by size:
4333 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2209 2824 2209
Size: 615 5263
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5878
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PspOMI G'GGCC_C
Cuts at: 3136 3136
Size: 5878
PstI C_TGCA'G
Cuts at: 830 2403 2628 3230 830
Size: 1573 225 602 3478
Fragments arranged by size:
3478 1573 602 225
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
PvuII CAG'CTG
Cuts at: 2338 2623 2338
Size: 285 5593
SacI G_AGCT'C
Cuts at: 2085 2085
Size: 5878
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SapI GCTCTTCn'nnn_
Cuts at: 2177 2177
Size: 5878
SbfI CC_TGCA'GG
Cuts at: 2403 3230 2403
Size: 827 5051
ScaI AGT'ACT
Cuts at: 4126 5838 4126
Size: 1712 4166
SfcI C'TryA_G
Cuts at: 826 1045 2399 2624 2943 3226 4361 5042 5233
Size: 219 1354 225 319 283 1135 681 191
Cuts at: 5233 826
Size: 1471
Fragments arranged by size:
1471 1354 1135 681 319 283 225 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5878
SmaI CCC'GGG
Cuts at: 1872 3205 1872
Size: 1333 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2660 3982 4883 5145 820
Size: 197 926 717 1322 901 262 1553
Fragments arranged by size:
1553 1322 926 901 717 262 197
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 3236 1132
Size: 2104 3774
SspI AAT'ATT
Cuts at: 5 52 3802 5
Size: 47 3750 2081
Fragments arranged by size:
3750 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5878
StyI C'CwwG_G
Cuts at: 513 1065 2097 2580 2599 2931 3317 513
Size: 552 1032 483 19 332 386 3074
Fragments arranged by size:
3074 1032 552 483 386 332 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3091 3905 3922 4075 4260 4705 325
Size: 1255 1511 814 17 153 185 445 1498
Fragments arranged by size:
1511 1498 1255 814 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3053 4124 5836
Size: 274 80 33 51 1443 1076 1071 1712
Cuts at: 5836 96
Size: 138
Fragments arranged by size:
1712 1443 1076 1071 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2048 2080 2360 2392 5838 5870 2048
Size: 32 280 32 3446 32 2056
Fragments arranged by size:
3446 2056 280 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5878
XbaI T'CTAG_A
Cuts at: 3214 3214
Size: 5878
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3148 2011
Size: 1137 4741
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 3203 1870
Size: 1333 4545
XmnI GAAnn'nnTTC
Cuts at: 2240 4007 2240
Size: 1767 4111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI