Restriction map for
pFN21AB6534
(Circular) (Six-base) MAPSORT of: pFN21AB6534.seq Check: 2077 from: 1 to: 5875
pFN21AB6534 5875 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3685 278
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3218 1937
Size: 1281 4594
Acc65I G'GTAC_C
Cuts at: 3196 3196
Size: 5875
AclI AA'CG_TT
Cuts at: 1563 4002 4375 1563
Size: 2439 373 3063
Fragments arranged by size:
3063 2439 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2517 3938 5322 5590 1632
Size: 695 190 1421 1384 268 1917
Fragments arranged by size:
1917 1421 1384 695 268 190
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5678
AflIII A'CryG_T
Cuts at: 1203 2837 2839 2895 4774 5751 1203
Size: 1634 2 56 1879 977 1327
Fragments arranged by size:
1879 1634 1327 977 56 2
AgeI A'CCGG_T
Cuts at: 4706 4706
Size: 5875
AhdI GACnn_n'nnGTC
Cuts at: 4604 4604
Size: 5875
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5875
AlwNI CAG_nnn'CTG
Cuts at: 3032 3618 5190 3032
Size: 586 1572 3717
Fragments arranged by size:
3717 1572 586
ApaLI G'TGCA_C
Cuts at: 3932 5088 3932
Size: 1156 4719
ApoI r'AATT_y
Cuts at: 1493 2008 2276 2378 2570 2600 3184 3410 3562
Size: 515 268 102 192 30 584 226 152
Cuts at: 3562 5671 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 584 515 268 226 192 152
102 30
AseI AT'TA_AT
Cuts at: 160 1032 4429 160
Size: 872 3397 1606
Fragments arranged by size:
3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
AvaI C'yCGr_G
Cuts at: 1870 1943 3200 1870
Size: 73 1257 4545
Fragments arranged by size:
4545 1257 73
BamHI G'GATC_C
Cuts at: 3205 3572 3205
Size: 367 5508
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3196 4651 618
Size: 325 217 627 1409 1455 1842
Fragments arranged by size:
1842 1455 1409 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3194 1391
Size: 1803 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4904
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4066 4100 1903
Size: 34 2129 34 3678
Fragments arranged by size:
3678 2129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3767 4977 3767
Size: 1210 4665
BclI T'GATC_A
Cuts at: 1548 2182 1548
Size: 634 5241
BfrBI ATG'CAT
Cuts at: 2297 2297
Size: 5875
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4486 136
Size: 107 122 71 631 605 2814 1525
Fragments arranged by size:
2814 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2722 5870 2722
Size: 3148 2727
BlpI GC'TnA_GC
Cuts at: 3292 3292
Size: 5875
Bme1580I G_kGCm'C
Cuts at: 1790 3936 5092 1790
Size: 2146 1156 2573
Fragments arranged by size:
2573 2146 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5875
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3606 4564 442
Size: 416 53 83 399 24 2189 958 1753
Fragments arranged by size:
2189 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2406 2696 2750 3003 4535 1239
Size: 1167 290 54 253 1532 2579
Fragments arranged by size:
2579 1532 1167 290 253 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2873 4000 4865 5163 2873
Size: 1127 865 298 3585
Fragments arranged by size:
3585 1127 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2768 2975 4538 882
Size: 617 1269 207 1563 2219
Fragments arranged by size:
2219 1563 1269 617 207
BsaAI yAC'GTr
Cuts at: 493 1204 2840 3666 493
Size: 711 1636 826 2702
Fragments arranged by size:
2702 1636 826 711
BsaBI GATnn'nnATC
Cuts at: 3061 3166 5869 3061
Size: 105 2703 3067
Fragments arranged by size:
3067 2703 105
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3682 4064 4699 275
Size: 53 83 186 936 2149 382 635 1451
Fragments arranged by size:
2149 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4308 4706 4980 5127 5538 4308
Size: 398 274 147 411 4645
Fragments arranged by size:
4645 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2953 2983 1178
Size: 30 165 30 1550 30 4070
Fragments arranged by size:
4070 1550 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3032 1186
Size: 1846 4029
BseYI C'CCAG_C
Cuts at: 5078 5574 5078
Size: 496 5379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2136 1163
Size: 573 400 4902
Fragments arranged by size:
4902 573 400
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3325 4086 5114 1540
Size: 275 186 1324 761 1028 2301
Fragments arranged by size:
2301 1324 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3936 4021 5092 3936
Size: 85 1071 4719
Fragments arranged by size:
4719 1071 85
BsmI GAATG_Cn'
Cuts at: 3386 3479 3386
Size: 93 5782
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3194 3936 4021 5092 1391
Size: 399 1404 742 85 1071 2174
Fragments arranged by size:
2174 1404 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5538 5538
Size: 5875
BspHI T'CATG_A
Cuts at: 3764 5494 3764
Size: 1730 4145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3230 844
Size: 2386 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3762 1394
Size: 429 1939 3507
Fragments arranged by size:
3507 1939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4370 4544 65
Size: 1183 3122 174 1396
Fragments arranged by size:
3122 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4519 4706 1700
Size: 110 2709 187 2869
Fragments arranged by size:
2869 2709 187 110
BsrGI T'GTAC_A
Cuts at: 96 2683 96
Size: 2587 3288
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5875
BssSI C'ACGA_G
Cuts at: 2972 3935 4947 2972
Size: 963 1012 3900
Fragments arranged by size:
3900 1012 963
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3032 794
Size: 2238 3637
BstBI TT'CG_AA
Cuts at: 4679 4679
Size: 5875
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5875
BstXI CCAn_nnnn'nTGG
Cuts at: 2698 2914 2698
Size: 216 5659
BstYI r'GATC_y
Cuts at: 1972 2722 3205 3572 3957 3974 5426 5512 5524
Size: 750 483 367 385 17 1452 86 12
Cuts at: 5524 5870 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 750 483 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2907 2965 513
Size: 552 1842 58 3423
Fragments arranged by size:
3423 1842 552 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5136
Fragments arranged by size:
5136 683 56
BtsI GCAGTG_nn'
Cuts at: 2148 2564 2787 3391 4185 4205 2148
Size: 416 223 604 794 20 3818
Fragments arranged by size:
3818 794 604 416 223 20
ClaI AT'CG_AT
Cuts at: 1554 2757 1554
Size: 1203 4672
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5840
DraI TTT'AAA
Cuts at: 3179 3179
Size: 5875
DraIII CAC_nnn'GTG
Cuts at: 2018 2018
Size: 5875
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4882 809
Size: 507 3566 1802
Fragments arranged by size:
3566 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2606 3322 4211
Size: 54 1425 50 275 18 776 716 889
Cuts at: 4211 5840 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 776 716 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3322 1537
Size: 275 1510 4090
Fragments arranged by size:
4090 1510 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2330 2772 2868 3081 3155 3805 1353
Size: 977 442 96 213 74 650 3423
Fragments arranged by size:
3423 977 650 442 213 96 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4462 4836 4982 387
Size: 1064 138 2873 374 146 1280
Fragments arranged by size:
2873 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 2406 2517 2696 2750 3003 3938
Size: 393 695 79 111 179 54 253 935
Cuts at: 3938 4535 5322 5590 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 935 787 695 597 393 268 253
179 111 79 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2993 2993
Size: 5875
EcoO109I rG'GnC_Cy
Cuts at: 2695 2695
Size: 5875
EcoRI G'AATT_C
Cuts at: 2008 2276 3184 3562 2008
Size: 268 908 378 4321
Fragments arranged by size:
4321 908 378 268
EcoRV GAT'ATC
Cuts at: 2923 2923
Size: 5875
FspI TGC'GCA
Cuts at: 4381 4381
Size: 5875
HaeII r_GCGC'y
Cuts at: 1678 5022 1678
Size: 3344 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2953 2985 4516 4548 4590
Size: 32 135 32 1381 32 1531 32 42
Cuts at: 4590 4622 1373
Size: 32 2626
Fragments arranged by size:
2626 1531 1381 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3219 3465 669
Size: 988 281 1281 246 3079
Fragments arranged by size:
3079 1281 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2264 2771 748
Size: 1516 507 3852
Fragments arranged by size:
3852 1516 507
HpaI GTT'AAC
Cuts at: 3465 3465
Size: 5875
KpnI G_GTAC'C
Cuts at: 3200 3200
Size: 5875
MfeI C'AATT_G
Cuts at: 3474 3474
Size: 5875
MluI A'CGCG_T
Cuts at: 5751 5751
Size: 5875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2077 4989 5173 1305
Size: 772 2912 184 2007
Fragments arranged by size:
2912 2007 772 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2608 10
Size: 54 1425 343 776 3277
Fragments arranged by size:
3277 1425 776 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2636 2735 2912 3833 4192 4351 518
Size: 648 1470 99 177 921 359 159 2042
Fragments arranged by size:
2042 1470 921 648 359 177 159 99
MspA1I CmG'CkG
Cuts at: 1606 2967 3038 3047 3291 3968 5116 5361 1606
Size: 1361 71 9 244 677 1148 245 2120
Fragments arranged by size:
2120 1361 1148 677 245 244 71 9
NcoI C'CATG_G
Cuts at: 513 1065 2907 513
Size: 552 1842 3481
Fragments arranged by size:
3481 1842 552
NdeI CA'TA_TG
Cuts at: 387 2299 387
Size: 1912 3963
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5875
NotI GC'GGCC_GC
Cuts at: 3322 3322
Size: 5875
NsiI A_TGCA'T
Cuts at: 2299 2299
Size: 5875
NspI r_CATG'y
Cuts at: 1132 1229 3233 4778 1132
Size: 97 2004 1545 2229
Fragments arranged by size:
2229 2004 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5875
PciI A'CATG_T
Cuts at: 4774 4774
Size: 5875
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5875
PfoI T'CCnGG_A
Cuts at: 2697 2697
Size: 5875
PmeI GTTT'AAAC
Cuts at: 3179 3179
Size: 5875
PmlI CAC'GTG
Cuts at: 2840 2840
Size: 5875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3944 3976 5457 5489 3944
Size: 32 1481 32 4330
Fragments arranged by size:
4330 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2695 2695
Size: 5875
PshAI GACnn'nnGTC
Cuts at: 1613 2840 1613
Size: 1227 4648
PsiI TTA'TAA
Cuts at: 3445 3445
Size: 5875
PstI C_TGCA'G
Cuts at: 830 3037 3227 830
Size: 2207 190 3478
Fragments arranged by size:
3478 2207 190
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5875
PvuII CAG'CTG
Cuts at: 3038 3038
Size: 5875
SacII CC_GC'GG
Cuts at: 2968 2968
Size: 5875
SalI G'TCGA_C
Cuts at: 1936 3217 1936
Size: 1281 4594
SapI GCTCTTCn'nnn_
Cuts at: 2330 2330
Size: 5875
SbfI CC_TGCA'GG
Cuts at: 3227 3227
Size: 5875
ScaI AGT'ACT
Cuts at: 4123 5835 4123
Size: 1712 4163
SfcI C'TryA_G
Cuts at: 826 1045 3033 3048 3223 4358 5039 5230 826
Size: 219 1988 15 175 1135 681 191 1471
Fragments arranged by size:
1988 1471 1135 681 219 191 175 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5875
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5875
SmaI CCC'GGG
Cuts at: 1872 3202 1872
Size: 1330 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2888 3979 4880 5142 820
Size: 197 926 945 1091 901 262 1553
Fragments arranged by size:
1553 1091 945 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3666 493
Size: 3173 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5875
SphI G_CATG'C
Cuts at: 1132 3233 1132
Size: 2101 3774
SspI AAT'ATT
Cuts at: 5 52 2360 2506 3799 5
Size: 47 2308 146 1293 2081
Fragments arranged by size:
2308 2081 1293 146 47
StuI AGG'CCT
Cuts at: 1517 2999 1517
Size: 1482 4393
StyI C'CwwG_G
Cuts at: 513 1065 2691 2907 3314 513
Size: 552 1626 216 407 3074
Fragments arranged by size:
3074 1626 552 407 216
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2443 3902 3919 4072 4257 4702 325
Size: 1255 863 1459 17 153 185 445 1498
Fragments arranged by size:
1498 1459 1255 863 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2683 4121 5833
Size: 274 80 33 51 1443 706 1438 1712
Cuts at: 5833 96
Size: 138
Fragments arranged by size:
1712 1443 1438 706 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5835 5867 5835
Size: 32 5843
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5875
XbaI T'CTAG_A
Cuts at: 3211 3211
Size: 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2914 2914
Size: 5875
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5875
XmaI C'CCGG_G
Cuts at: 1870 3200 1870
Size: 1330 4545
XmnI GAAnn'nnTTC
Cuts at: 4004 4004
Size: 5875
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3683 276
Size: 53 83 186 936 2149 2468
Fragments arranged by size:
2468 2149 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PspOMI PspXI PsrI RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI