Restriction map for
pFN21AB6527
(Circular) (Six-base) MAPSORT of: pFN21AB6527.seq Check: 8110 from: 1 to: 5800
pFN21AB6527 5800 bp
With 182 enzymes: SgfI *
October 20, 2009 10:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2939 2939
Size: 5800
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2844 3610 278
Size: 53 83 186 936 1308 766 2468
Fragments arranged by size:
2468 1308 936 766 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3143 1937
Size: 1206 4594
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2636 2972 3044 3056 3863 5247 5515 1632
Size: 1004 336 72 12 807 1384 268 1917
Fragments arranged by size:
1917 1384 1004 807 336 268 72 12
AfeI AGC'GCT
Cuts at: 2126 2126
Size: 5800
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5603
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 2460 1166
Size: 1294 4506
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5800
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2262 2294 2262
Size: 32 5768
AlwNI CAG_nnn'CTG
Cuts at: 3543 5115 3543
Size: 1572 4228
ApaLI G'TGCA_C
Cuts at: 3857 5013 3857
Size: 1156 4644
ApoI r'AATT_y
Cuts at: 1493 3109 3335 3487 5596 1493
Size: 1616 226 152 2109 1697
Fragments arranged by size:
2109 1697 1616 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 2228 2365 3125 1870
Size: 73 285 137 760 4545
Fragments arranged by size:
4545 760 285 137 73
BamHI G'GATC_C
Cuts at: 2306 3130 3497 2306
Size: 824 367 4609
Fragments arranged by size:
4609 824 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2052 2361 2502 2775 3121
Size: 325 217 627 265 309 141 273 346
Cuts at: 3121 4576 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 346 325 309 273 265
217 141
BanII G_rGCy'C
Cuts at: 1391 2072 2238 2628 3119 1391
Size: 681 166 390 491 4072
Fragments arranged by size:
4072 681 491 390 166
BbeI G_GCGC'C
Cuts at: 2056 2056
Size: 5800
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4829
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3991 4025 1903
Size: 34 2054 34 3678
Fragments arranged by size:
3678 2054 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5800
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4411 136
Size: 107 122 71 631 605 337 2402 1525
Fragments arranged by size:
2402 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 2209 2778 3861 5017 1790
Size: 419 569 1083 1156 2573
Fragments arranged by size:
2573 1156 1083 569 419
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2203 3531 4489
Size: 416 53 83 399 24 786 1328 958
Cuts at: 4489 442
Size: 1753
Fragments arranged by size:
1753 1328 958 786 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3073 3073
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3038 4460 1239
Size: 1799 1422 2579
Fragments arranged by size:
2579 1799 1422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3925 4790 5088 3925
Size: 865 298 4637
Fragments arranged by size:
4637 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4463 882
Size: 617 2964 2219
Fragments arranged by size:
2964 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2731 3591 493
Size: 711 1527 860 2702
Fragments arranged by size:
2702 1527 860 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2053 2100 2547 2658
Size: 53 83 186 936 520 47 447 111
Cuts at: 2658 2841 3607 3989 4624 275
Size: 183 766 382 635 1451
Fragments arranged by size:
1451 936 766 635 520 447 382 186
183 111 83 53 47
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5575
Fragments arranged by size:
5575 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5800
BseYI C'CCAG_C
Cuts at: 2017 2713 3069 5003 5499 2017
Size: 696 356 1934 496 2318
Fragments arranged by size:
2318 1934 696 496 356
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2557 1163
Size: 573 821 4406
Fragments arranged by size:
4406 821 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3250 4011 5039 1540
Size: 275 186 1249 761 1028 2301
Fragments arranged by size:
2301 1249 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2193 2628 2739 3861 3946 5017 2193
Size: 435 111 1122 85 1071 2976
Fragments arranged by size:
2976 1122 1071 435 111 85
BsmI GAATG_Cn'
Cuts at: 2431 3311 3404 2431
Size: 880 93 4827
Fragments arranged by size:
4827 880 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 2193 2209 2238 2628 2739 2778
Size: 399 282 121 16 29 390 111 39
Cuts at: 2778 3119 3861 3946 5017 1391
Size: 341 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 390 341 282 121
111 85 39 29 16
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2939 3155 844
Size: 2095 216 3489
Fragments arranged by size:
3489 2095 216
BsrBI CCG'CTC
Cuts at: 1394 1823 3687 1394
Size: 429 1864 3507
Fragments arranged by size:
3507 1864 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2176 4295 4469 65
Size: 1183 928 2119 174 1396
Fragments arranged by size:
2119 1396 1183 928 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 2061 2429 2445 2682 2778 4444
Size: 110 236 15 368 16 237 96 1666
Cuts at: 4444 4631 1700
Size: 187 2869
Fragments arranged by size:
2869 1666 368 237 236 187 110 96
16 15
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5800
BssSI C'ACGA_G
Cuts at: 3860 4872 3860
Size: 1012 4788
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5800
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstXI CCAn_nnnn'nTGG
Cuts at: 2479 2479
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 2306 3130 3497 3882 3899 5351 5437 5449
Size: 334 824 367 385 17 1452 86 12
Cuts at: 5449 5795 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 824 385 367 346 334 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2082 513
Size: 552 939 78 4231
Fragments arranged by size:
4231 939 552 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2889 487
Size: 683 56 1663 3398
Fragments arranged by size:
3398 1663 683 56
BtsI GCAGTG_nn'
Cuts at: 2191 2729 3316 4110 4130 2191
Size: 538 587 794 20 3861
Fragments arranged by size:
3861 794 587 538 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2106 2329
Size: 54 1425 50 275 18 198 78 223
Cuts at: 2329 3247 4136 5765 8
Size: 918 889 1629 43
Fragments arranged by size:
1629 1425 918 889 275 223 198 78
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3247 1537
Size: 275 1435 4090
Fragments arranged by size:
4090 1435 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2243 2837 3730 1353
Size: 890 594 893 3423
Fragments arranged by size:
3423 893 890 594
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2626 2626
Size: 5800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2636 2972 3038 3044 3056 3863 4460
Size: 393 1004 336 66 6 12 807 597
Cuts at: 4460 5247 5515 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1004 807 787 597 393 336 268
66 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2894 2894
Size: 5800
EcoO109I rG'GnC_Cy
Cuts at: 2476 2673 2797 2476
Size: 197 124 5479
Fragments arranged by size:
5479 197 124
EcoRI G'AATT_C
Cuts at: 3109 3487 3109
Size: 378 5422
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 2056 2128 4947 1678
Size: 378 72 2819 2531
Fragments arranged by size:
2819 2531 378 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2867 2899 3077 3109 4441
Size: 32 135 32 1295 32 178 32 1332
Cuts at: 4441 4473 4515 4547 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1332 1295 178 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2164 2707 3144 3390 669
Size: 988 281 226 543 437 246 3079
Fragments arranged by size:
3079 988 543 437 281 246 226
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KasI G'GCGC_C
Cuts at: 2052 2052
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2076 3027 4914 5098 1305
Size: 771 951 1887 184 2007
Fragments arranged by size:
2007 1887 951 771 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 10
Size: 54 1425 343 276 3702
Fragments arranged by size:
3702 1425 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2460 3758 4117 4276 518
Size: 648 1294 1298 359 159 2042
Fragments arranged by size:
2042 1298 1294 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2379 2500 3016 3216 3893 5041 5286 1606
Size: 773 121 516 200 677 1148 245 2120
Fragments arranged by size:
2120 1148 773 677 516 245 200 121
NaeI GCC'GGC
Cuts at: 2431 2447 2684 2431
Size: 16 237 5547
Fragments arranged by size:
5547 237 16
NarI GG'CG_CC
Cuts at: 2053 2053
Size: 5800
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4309
Fragments arranged by size:
4309 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NgoMIV G'CCGG_C
Cuts at: 2429 2445 2682 2429
Size: 16 237 5547
Fragments arranged by size:
5547 237 16
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 3247 3247
Size: 5800
NspI r_CATG'y
Cuts at: 1132 1229 3158 4703 1132
Size: 97 1929 1545 2229
Fragments arranged by size:
2229 1929 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5800
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PmlI CAC'GTG
Cuts at: 2731 2731
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3869 3901 5382 5414 3869
Size: 32 1481 32 4255
Fragments arranged by size:
4255 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PstI C_TGCA'G
Cuts at: 830 2858 3152 830
Size: 2028 294 3478
Fragments arranged by size:
3478 2028 294
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
SacI G_AGCT'C
Cuts at: 2628 2628
Size: 5800
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SapI GCTCTTCn'nnn_
Cuts at: 2243 2837 2243
Size: 594 5206
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 2264 4048 5760 2264
Size: 1784 1712 2304
Fragments arranged by size:
2304 1784 1712
SexAI A'CCwGG_T
Cuts at: 2508 2508
Size: 5800
SfcI C'TryA_G
Cuts at: 826 1045 2854 3148 4283 4964 5155 826
Size: 219 1809 294 1135 681 191 1471
Fragments arranged by size:
1809 1471 1135 681 294 219 191
SfoI GGC'GCC
Cuts at: 2054 2054
Size: 5800
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 2367 3127 1872
Size: 495 760 4545
Fragments arranged by size:
4545 760 495
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3904 4805 5067 820
Size: 197 926 1961 901 262 1553
Fragments arranged by size:
1961 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 2900 1517
Size: 1383 4417
StyI C'CwwG_G
Cuts at: 513 1065 2004 2103 2472 3075 3239 513
Size: 552 939 99 369 603 164 3074
Fragments arranged by size:
3074 939 603 552 369 164 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2009 2591 3827 3844 3997 4182 4627
Size: 1255 429 582 1236 17 153 185 445
Cuts at: 4627 325
Size: 1498
Fragments arranged by size:
1498 1255 1236 582 445 429 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2262 2487 4046
Size: 274 80 33 51 1443 285 225 1559
Cuts at: 4046 5758 96
Size: 1712 138
Fragments arranged by size:
1712 1559 1443 285 274 225 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5760 5792 5760
Size: 32 5768
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5800
XmaI C'CCGG_G
Cuts at: 1870 2365 3125 1870
Size: 495 760 4545
Fragments arranged by size:
4545 760 495
XmnI GAAnn'nnTTC
Cuts at: 2765 3929 2765
Size: 1164 4636
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2842 3608 276
Size: 53 83 186 936 1308 766 2468
Fragments arranged by size:
2468 1308 936 766 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SfiI SrfI SwaI XcmI